Effect of AndoSan™ on expression of adhesion molecules and production of reactive oxygen species in human monocytes and granulocytes in vivo.

Abstract:

BACKGROUND:Oral intake (60 ml daily) over 12 days in eight healthy volunteers of an immunostimulatory extract based on the medicinal mushroom Agaricus blazei Murill (AbM (AndoSan™)), reduced the monocyte and granulocyte release of mainly proinflammatory cytokines in vivo, suggesting an anti-inflammatory effect. In this foremost in vivo study, the aim was to examine the effect of such AndoSan™ consumption on the expression of adhesion molecules CD11b, CD11c and CD62L and production of reactive oxygen species (ROS) in leukocytes. METHODOLOGY/PRINCIPAL FINDINGS:As shown by flow cytometry, there was a significant increase of CD62L expression on monocytes and granulocytes from before (day 0) compared with 12 days after daily AndoSan™ consumption. However, only minor alterations and no clear trend in the expression of CD11b and CD11c were detected. Intracellular ROS (mainly superoxide ion) were significantly reduced in these cells from days 0 to 12. CONCLUSIONS/SIGNIFICANCE:These results support that oral intake of AndoSan™ exhibits an anti-inflammatory effect in humans in vivo.

journal_name

Scand J Gastroenterol

authors

Johnson E,Førland DT,Hetland G,Sætre L,Olstad OK,Lyberg T

doi

10.3109/00365521.2012.660544

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

984-92

issue

8-9

eissn

0036-5521

issn

1502-7708

journal_volume

47

pub_type

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