Abstract：
:It is our hypothesis that interstitial fluid flow plays a role in the bone remodeling response to mechanical loading. The fluid flow-induced expression of three proteins (collagen, osteopontin, and alkaline phosphatase) involved in bone remodeling was investigated. Rat calvarial osteoblasts subjected to pulsatile fluid flow at an average shear stress of 5 dyne/cm2 showed decreased alkaline phosphatase (AP) mRNA expression after only 1 hour of flow. After 3 hours of flow, AP mRNA levels had decreased to 30% of stationary control levels and remained at this level for an additional 5 hours of flow. Steady flow (4 dyne/cm2 fluid shear stress), in contrast, resulted in a delayed and less dramatic decrease in AP mRNA expression to 63% of control levels after 8 hours of flow. The reduced AP mRNA expression under pulsatile flow conditions was followed by reduced AP enzyme activity after 24 hours. No changes in collagen or osteopontin mRNA expression were detected over 8 hours of pulsatile flow. This is the first time fluid flow has been shown to affect gene expression in osteoblasts.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Hillsley MV,Frangos JAdoi
10.1007/s002239900185subject
Has Abstractpub_date
1997-01-01 00:00:00pages
48-53issue
1eissn
0171-967Xissn
1432-0827journal_volume
60pub_type
杂志文章abstract：:There is increasing evidence that 1,25 dihydroxyvitamin D3 acts directly to differentiate and activate cells of mononuclear phagocyte lineage (MNP). However, it seems possible that in bone marrow this hormone may modulate the differentiation of MNP via the factor elaborated by the stromal cells or the macrophages. We ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556592
更新日期：1986-01-01 00:00:00
abstract：:Osteoporosis is a common age-related disease with a strong genetic influence. COLIA1 is one of the most extensively studied candidate genes and has consistently been associated with BMD and fracture. We examined the effects of the polymorphisms -1997G>T, -1663indelT, and +1245G>T and their haplotypes on vertebral frac...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9199-1
更新日期：2009-02-01 00:00:00
abstract：:Measurements of broadband ultrasonic attenuation (BUA) represent an established means of skeletal status assessment in osteoporosis. Today, the skeletal site that is the most widely used clinically is the calcaneus (heel bone). However, we hypothesized that fracture risk predictions could be improved if ultrasound mea...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-004-0216-8
更新日期：2004-11-01 00:00:00
abstract：:Further insight into human tooth enamel, dense fraction (TE), has been obtained by following the change and loss of CO3(2-), OH-, structurally incorporated H2O, Cl-, and, indirectly, HPO4(2-) after TE had been heated in N2 or vacuum in the range 25-1000 degrees C. Quantitative infrared spectroscopic, lattice parameter...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02407181
更新日期：1980-01-01 00:00:00
abstract：:Allogenous demineralized bone matrix (DBM) represents a potential scaffold for bone tissue engineering due to its close relation in structure and function with autologous bone, but its supply is often restricted by donor availability. Thus, an expanded source of human bone is needed. The aim of this study was to evalu...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9159-9
更新日期：2008-09-01 00:00:00
abstract：:Aluminum ions (Al) and chromium (III) ions (Cr), as they exist in aqueous solution at neutral pH, adsorb onto calcium hydroxyapatite crystals (HAP) and severely inhibit their dissolution process, when present in concentrations less than 1 microM. Iron (III) ions (Fe), at concentrations up to 10 microM, have no effect ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555127
更新日期：1987-07-01 00:00:00
abstract：:Radioiodination of synthetic human 37-49 osteocalcin requires optimal labeling conditions in order to obtain a maximum of mono- and di-iodinated tracer with little contamination by tri- and tetra-iodinated products or "radio-damage." The antibody raised against osteocalcin(h37-49) had the highest affinity for the C-te...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555196
更新日期：1986-11-01 00:00:00
abstract：:Because of the variability of collagen crosslinks, their use as markers for bone resorption is often criticized. We hypothesized that the variability could be reduced by collecting urine for 24 hours (or longer) instead of using single voids, and by not normalizing to creatinine. Urine samples were collected from 22 h...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-003-0149-7
更新日期：2004-04-01 00:00:00
abstract：:Insulin is a potent stimulator of collagen synthesis and other osteoblastic cell functions. In various insulin-sensitive tissues, stimulation of glucose transport and glycolytic metabolism are hallmarks of insulin action and may play a role in insulin regulation of cellular function. However, the effects of insulin on...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556657
更新日期：1989-07-01 00:00:00
abstract：:The hypophosphatemic male mouse (Hyp/y), the proposed model for human vitamin D-resistant rickets (VDRR), is characterized by chronic hypophosphatemia, dwarfism, and rachitic and osteomalacic bone lesions. We have reported that treatment of Hyp/y mice with phosphate salts (Pi) heals rickets but does not correct the de...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411227
更新日期：1982-03-01 00:00:00
abstract：:The metacarpal bone mineral density (BMD) and metacarpal index (MCI) of the second metacarpal bone were measured by computed X-ray densitometry (CXD) (Teijin Ltd., Tokyo), which we have established with the development of microdensitometry of radiographs. In this study, we evaluated the basic attributes of this CXD me...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00299308
更新日期：1994-11-01 00:00:00
abstract：:Ultrasonic methods measure properties intrinsic to bone's structure that are not necessarily dependent on bone mass. Therefore, ultrasound may prove to be a useful tool for diagnosing bone fragility and osteoporosis. The goal of this study was to determine the relationship between apparent velocity of ultrasound (AVU)...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02565132
更新日期：1991-08-01 00:00:00
abstract：:The demonstration of histochemical characteristics in calcifying cartilage is fraught with methodological difficulties including the distinction of mineralized from unmineralized cartilage and the demonstration of cell detail in relatively hard tissue. This study uses the decalcified bone matrix-induced enchondral (en...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555141
更新日期：1988-10-01 00:00:00
abstract：:Dual photon absorptiometry (DPA) is currently the most widely used method for noninvasive bone mineral density (BMD) measurement of the axial skeleton. Dual energy X-ray absorptiometry (DEXA) is a recently developed technique that uses an X-ray tube as a photon source; it has demonstrated several significant advantage...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556250
更新日期：1991-11-01 00:00:00
abstract：:The type IA osteogenesis imperfecta (OI) phenotype is characterized by multiple fractures, blue sclerae, and minimal skeletal deformity without dentinogenesis imperfecta. The object of this study was to determine the effect of treatment with intravenous pamidronate (30 mg) every 3 months on bone density and bone histo...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章
doi：10.1007/s00223-001-1055-5
更新日期：2003-02-01 00:00:00
abstract：:Correlated studies were performed with light and electron microscopy, and backscattered electron image in conjunction with X-ray microanalysis, of lanthanum-incubated epiphyseal cartilage of the young rat. The hall-mark of this procedure is the appearance of LaP electron-dense deposits (not present in control sections...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02508648
更新日期：1996-04-01 00:00:00
abstract：:There is increasing interest in identifying new pathways and candidate genes that confer susceptibility to osteoporosis. There is evidence that adipogenesis and osteogenesis may be related, including a common bone marrow progenitor cell for both adipocytes and osteoblasts. Perilipin 1 (PLIN1) and Perilipin 4 (PLIN4) a...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9552-7
更新日期：2012-02-01 00:00:00
abstract：:The crystallinity of bone mineral at different stages of maturation has been measured by quantitative X-ray diffraction methods. Crystallinity measurements were made on tibial mid-diaphyses from 17-day embryonic chicks, newly-formed periosteal bone from embryonic chicks, and density-fractionated bone from post-hatch c...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405032
更新日期：1983-01-01 00:00:00
abstract：:The main congenital conditions of hypophosphatemia expressed in adulthood include several forms of hereditary hypophosphatemic rickets and a congenital disorder of vitamin D metabolism characterized by osteomalacia and hypophosphatemia in adult patients. Hypophosphatemia in adults is defined as serum phosphate concent...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-020-00695-2
更新日期：2021-01-01 00:00:00
abstract：:This study sought to develop a noninvasive, reliable, clinically relevant, and easy-to-implement mouse model that can be used for investigation of the pathophysiology of PTOA and for preclinical testing of new therapies of PTOA. Accordingly, we have established a closed intraarticular tibial plateau compression loadin...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00614-0
更新日期：2020-02-01 00:00:00
abstract：:At 4 and 24 hours after injection of (35S)sulfate, there were 22-67% fewer silver grains on conventionally fixed sections than on sections fixed by cryotechniques. Differences were smaller when (3H) glucosamine was chosen as precursor. The number of silver grains increased between 4 and 24 hours in predentine and in a...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02557834
更新日期：1985-09-01 00:00:00
abstract：:The purpose of this study was to estimate clinical validity of a new available immunoradiometric assay for circulating intact human BGP (N-tact Osteo SP) by measuring this protein in a large number of normal subjects and patients with the most common metabolic bone diseases. One hundred normal subjects were studied in...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/pl00005815
更新日期：1999-05-01 00:00:00
abstract：:Adiponectin, a protein classically known to be secreted by adipocytes, is also secreted by bone-forming cells. Results of previous studies have been contradictory as to whether serum adiponectin and bone mineral density (BMD) are associated. The aim of this study was to investigate a possible association between serum...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9376-x
更新日期：2010-07-01 00:00:00
abstract：:The aims of this study were to compare the response to therapy in disease activity in pagetic patients with and without skull involvement and the usefulness of bone markers in the evaluation of these patients. Forty patients with Paget's disease treated with tiludronate and 26 healthy controls were included. Serum tot...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-0247-9
更新日期：2006-07-01 00:00:00
abstract：:Data on the association between dietary vitamin K intake and fracture risk are limited among Chinese. This study examined such an association in community-dwelling elderly in Hong Kong. We present data from 2,944 subjects (1,605 men, 1,339 women) who participated in a prospective cohort study. Baseline dietary intakes...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-012-9586-5
更新日期：2012-05-01 00:00:00
abstract：:This cross-sectional, observational study was designed to identify clinical risk factors of osteoporosis and fractures in Korean women to validate the probability of osteoporosis and subsequent fractures. A total of 1541 Korean women were recruited nationally. Fracture history of any site, risk factors of osteoporosis...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9182-x
更新日期：2008-12-01 00:00:00
abstract：:Tumor tissue from a patient with squamous cell carcinoma of the lung and hypercalcemia has been serially implanted into athymic mice. Tumor-bearing mice develop cachexia, hypercalcemia without bone metastases, hypophosphatemia, increased urinary cyclic adenosine monophosphate (cAMP) to creatinine ratio, and undetectab...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405367
更新日期：1984-09-01 00:00:00
abstract：:Condylar cartilages were cultured in the form of organ cultures on top of collagen sponges in medium containing 2% fetal calf serum and were treated with 3.5-350 nM insulin for 6 days. Doses of 175 nM of insulin caused a marked increase (+96%) in DNA synthesis and in proteoglycan production (+74%), features that manif...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00296654
更新日期：1993-04-01 00:00:00
abstract：:The aim of our study was to compare the results provided by the measurement of vertebral and femoral bone mineral density (BMD) for assessing the individual risk of osteoporosis as defined by either low BMD and/or rapid bone loss. Vertebral and femoral BMD were measured twice at a mean interval of 21 months in 85 norm...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00310196
更新日期：1993-05-01 00:00:00
abstract：:Calcium deficiency is a major etiological determinant of rickets in Nigerian children and is accompanied by undermineralization of the developing bone matrix which is composed largely of type I collagen. We have assessed types I and III collagen metabolism by measuring the circulating concentrations of teh N- and C-te...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900301
更新日期：1997-08-01 00:00:00
