Abstract：
:The immunogenicity and protective efficacy of a nucleic acid vaccine encoding the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) gene under the control of the CMV immediate early gene promoter was examined. Mice immunized three times by intramuscular injection with the vaccine developed an HSV specific IgG but not IgA antibody response detectable in both serum and vaginal secretions. In addition, antigen-specific cellular immune responses were detected in splenic lymphocytes isolated from DNA immunized animals. Immunization reduced virus replication in the genital tract following a lethal intravaginal HSV-2 challenge. Furthermore, symptomatic genital HSV disease was reduced in immunized mice and the animals were completely protected from death. We conclude that a nucleic acid vaccine expressing HSV-1 gD induced both humoral and cell mediated immune responses in mice which proved highly protective against disease following virus challenge.
journal_name
Vaccinejournal_title
Vaccineauthors
Bourne N,Milligan GN,Schleiss MR,Bernstein DI,Stanberry LRdoi
10.1016/s0264-410x(96)00027-8subject
Has Abstractpub_date
1996-09-01 00:00:00pages
1230-4issue
13eissn
0264-410Xissn
1873-2518pii
S0264410X96000278journal_volume
14pub_type
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