Abstract:
:In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of the 3'-7kb region, the CK/CH/LHLJ/04V virus population contained subpopulations with a mixture of genetic mutants. The titers of the virus increased gradually during serial passage, but the replication capacity decreased in chickens. The virus was partially attenuated at passage 40 (P40) and P70, and was fully attenuated at P110. It lost immunogenicity and kidney tropism at P110 and P70, respectively. Amino acid substitutions were found in the 3'-7kb region, primarily in the spike (S) protein. Substitutions in the S1 subunit occurred between P3 and P40 and all subpopulations in a virus passage showed the same substitutions. Other substitutions that occurred between P70 and P110, however, were found only in some subpopulations of the virus passages. A 109-bp deletion in the 3'-UTR was observed in most subpopulations of P70 and P110, and might be related to virus replication, transcription and pathogenicity. The changes described in the 3'-7kb region of the virus are possibly responsible for virus attenuation, immunogenicity decrease and tissue tropism changes; however, we cannot exclude the possibility that other parts of the genome may also be involved in those changes.
journal_name
Vaccinejournal_title
Vaccineauthors
Liu S,Zhang X,Gong L,Yan B,Li C,Han Z,Shao Y,Li H,Kong Xdoi
10.1016/j.vaccine.2009.05.072subject
Has Abstractpub_date
2009-07-23 00:00:00pages
4630-40issue
34eissn
0264-410Xissn
1873-2518pii
S0264-410X(09)00807-Xjournal_volume
27pub_type
杂志文章相关文献
VACCINE文献大全abstract:BACKGROUND AND PURPOSE:Varicella disease is a risk factor for pediatric Arterial Ischemic Stroke (AIS). Isolated case reports have emerged suggesting that varicella vaccination may also pose a risk for AIS. METHODS:This retrospective population-based cohort study assessed the risk of AIS in children who received a var...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.04.012
更新日期:2018-05-11 00:00:00
abstract::A growing number of people use the Internet to obtain health information, including information about vaccines. Websites that allow and promote interaction among users are an increasingly popular source of health information. Users of such so-called Web 2.0 applications (e.g. social media), while still in the minority...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.02.025
更新日期:2012-05-28 00:00:00
abstract::Human respiratory syncytial virus (RSV) is a leading cause of severe respiratory disease and hospitalizations in infants and young children. It also causes significant morbidity and mortality in elderly and immune compromised individuals. No licensed vaccine currently exists. Parainfluenza virus 5 (PIV5) is a paramyxo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.03.049
更新日期:2014-05-23 00:00:00
abstract::Foot and Mouth Disease (FMD) is an acute disease caused by Foot and Mouth Disease Virus (FMDV) which causes important economy losses, this is why it is necessary to obtain a vaccine that stimulates a rapid and long-lasting protective immune response. Cliptox is a mineral microparticle that in earlier studies has shown...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.06.098
更新日期:2010-08-31 00:00:00
abstract::We present the burden of human papillomavirus (HPV)-related cancers (cancers of the cervix, anogenital areas, and oral cavity and pharynx) in terms of incidence and mortality, for the countries of the Asia Pacific region. The region contains more than half of the world population, and manifests a wide geographic diver...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.05.010
更新日期:2008-08-19 00:00:00
abstract::Financial sustainability plans (FSPs) were developed by over 50 of the world's poorest countries receiving funding support from the Global Alliance for Vaccines and Immunization (GAVI) to introduce new and underused vaccines, injection safety and immunization service support between 2000 and 2006. These plans were ana...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2008.10.014
更新日期:2008-12-02 00:00:00
abstract::Polymeric nanoparticles (NPs) of poly (lactic-co-glycolic) acid (PLGA) possess adjuvant properties. To date, there are few studies exploring their application as antigen carriers for vaccination of fish. This study presents a preclinical assessment of the early innate and adaptive immune responses in Atlantic salmon f...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.08.087
更新日期:2011-10-26 00:00:00
abstract::The protective capacity of many currently used vaccines is based on induction of neutralizing antibodies. Many pathogens, however, have adapted themselves in different ways to escape antibody-based immune protection. In particular, for those infections against which conventional neutralizing antibody-based vaccination...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2010.08.101
更新日期:2010-11-16 00:00:00
abstract::Peripheral blood mononuclear cells were taken from subjects before and after oral immunization with cholera toxin B-subunit. Cells obtained from naive volunteers before immunization did not proliferate in vitro to B-subunit. Oral immunization induced a proliferative response in all volunteers with a peak stimulation i...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/0264-410x(93)90005-i
更新日期:1993-01-01 00:00:00
abstract::We have evaluated in C57/Bl6 and HLA-A2.1 transgenic mice the immunogenicity of three MVA vectors expressing either native HCV E1E2 polyprotein, truncated and secreted E1 (E'1(311)) and E2 (E'2(661)) proteins, or a chimeric E1E2 heterodimer presented at the plasma membrane. Immunization induced mainly a Th1 response i...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.04.005
更新日期:2004-09-28 00:00:00
abstract::Since smallpox eradication by the WHO during the 1980s, potency of new vaccines is compared to vaccines that were used during the eradication campaign. In this work we characterize the tail scarification technique in mice as a model for scarification in humans. Similar to humans, mice develop "clinical take" which is ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.09.023
更新日期:2007-11-07 00:00:00
abstract::The neuraminidase (NA) protein of influenza A viruses (IAV) has important functional roles in the viral replication cycle. Antibodies specific to NA can reduce viral replication and limit disease severity, but are not routinely measured. We analyzed NA inhibiting (NI) antibody titers in serum and respiratory specimens...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.06.001
更新日期:2016-07-19 00:00:00
abstract::The immunogenicity of 23-valent pneumococcal polysaccharide vaccine was assessed in 57 HIV-1 infected former intravenous drug users and in 20 HIV-1 negative controls. The effect of vaccination on HIV-1 infection was studied in a subgroup of 38 patients, 60% of whom under highly active antiretroviral therapy (HAART). A...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00357-2
更新日期:2002-11-01 00:00:00
abstract::Oral poliovirus vaccine (OPV) is tested for safety by evaluation of neurovirulence in rhesus and cynomolgus macaques. After intraspinal or intrathalamic injection of varying doses of vaccine, monkeys are followed for 17-21 days, killed, and a histopathological evaluation is made of the severity of poliomyelitis lesion...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(92)90396-2
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Surveillance for acute flaccid paralysis with laboratory confirmation has been a key strategy in the global polio eradication initiative, and the laboratory platform established for polio testing has been expanded in many countries to include surveillance for cases of febrile rash illness to identify measles...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.01.004
更新日期:2015-02-25 00:00:00
abstract:OBJECTIVES:Health care providers (HCP) are at high risk of acquiring and transmitting pertussis to susceptible family members, co-workers, and patients. Public health authorities recommend administering a single dose of Tdap (tetanus toxoid, reduced diphtheria toxoid, and acellular pertussis) vaccine to all adults, inc...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.11.071
更新日期:2018-01-04 00:00:00
abstract::A human hookworm vaccine is under development and in clinical trials in Africa and the Americas. The vaccine contains the Na-APR-1 and Na-GST-1 antigens. It elicits neutralizing antibodies that interfere with establishment of the adult hookworm in the gut and the ability of the parasite to feed on blood. The vaccine t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.03.078
更新日期:2016-06-03 00:00:00
abstract::The comparison between fetal and maternal levels of immunoglobulins indicate that the human placenta during pregnancy develops a specific transport mechanism for IgG in the maternal to fetal direction. There are differences for the four subclasses, with preferential transfer of IgG(1) while the slowest transfer is see...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/s0264-410x(03)00333-5
更新日期:2003-07-28 00:00:00
abstract::We have shown previously that expression library immunization is viable alternative approach to induce protective immunity against Neisseria meningitidis serogroup B. In this study we report that few rounds of library screening allow identification of protective pools of defined antigens. A previously reported protect...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.04.084
更新日期:2007-07-09 00:00:00
abstract:BACKGROUND:Adjuvants like AS01B increase the immunogenicity of vaccines and generally cause increased transient reactogenicity compared with Alum. A phase II randomized trial was conducted to characterize the response to AS01B and Alum adjuvanted vaccines. A post-hoc analysis was performed to examine the associations b...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2019.02.015
更新日期:2019-03-28 00:00:00
abstract:OBJECTIVES:Persistent hepatitis B virus (HBV) infection is a major cause of morbidity and mortality in sub-Saharan Africa. The HIV epidemic has the potential to affect its biology. Immunisation protocols established in the pre-HIV era are based upon data showing predominantly horizontal infant transmission. This study ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.08.028
更新日期:2013-11-12 00:00:00
abstract::The ability of ALVAC- or fowlpox-based simian immunodeficiency virus (SIV) vaccines to boost SIV-specific CD4+ and CD8+ T-cell responses was tested in 10 vaccinia-experienced macaques infected with SIVmac251. The CD8+ T-cell response to the dominant Gag(181-189) CM9 was quantitated in seven Mamu-A*01-positive macaques...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.08.028
更新日期:2004-01-26 00:00:00
abstract::Respiratory syncytial virus (RSV) is a leading viral cause of respiratory morbidity and mortality in infants and young children worldwide. Low and middle income countries (LMICs) account for approximately 99% of the global mortality estimates in this population, with up to 200,000 RSV deaths per year. The vaccine prod...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2017.02.068
更新日期:2019-11-28 00:00:00
abstract::During the endgame of global polio eradication, the universal introduction of inactivated poliovirus vaccines is urgently required to reduce the risk of vaccine-associated paralytic poliomyelitis and polio outbreaks due to wild and vaccine-derived polioviruses. In particular, the development of inactivated poliovirus ...
journal_title:Vaccine
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.vaccine.2014.11.015
更新日期:2016-04-07 00:00:00
abstract::Helper T cel lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. A line stimulated in vitro with Mem 71-Bel virus was able to proliferate...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(85)90118-5
更新日期:1985-09-01 00:00:00
abstract:BACKGROUND:Immunization with pneumococcal vaccines is an important prophylactic strategy for children with asplenia or splenic dysfunction, who are at high risk of bacterial infections (including S. pneumoniae). This study aimed to assess immunogenicity and safety of pneumococcal non-typeable Haemophilus influenzae pro...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.vaccine.2017.08.039
更新日期:2017-09-25 00:00:00
abstract::To evaluate the immunogenicity of 23-valent pneumococcal polysaccharide vaccine in 52 nursing homes residents aged > or = 60 years, IgG antibodies to serotypes 1, 5, 6B, and 8 were measured by ELISA and compared before, and 1 and 12 months following vaccination. A significant immunological response for all serotypes w...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.07.024
更新日期:2004-12-21 00:00:00
abstract::Prevalence of non-typeable Haemophilus influenzae (NTHi) in the etiology of invasive infections in immunocompromised individuals is increasing. Serum IgG antibody levels to H. influenzae protein D (PD) were significantly lower in adults suffering from chronic conditions causing secondary immunodeficiency (COPD, cancer...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.12.113
更新日期:2012-02-08 00:00:00
abstract::Chagas' disease is a major tropical disease for which a cure for chronic phase does not exist yet. Trypanosoma cruzi trans-sialidase (TS) seems to be involved in relevant processes such as infectivity, host survival and, very importantly, disease pathogenesis. In this study, we show that mice vaccinated with an engine...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.02.060
更新日期:2008-05-02 00:00:00
abstract::The Decade of Vaccines (DoV) initiative, launched in 2010, has as its mission "to extend, by 2020 and beyond, the full benefits of immunization to all people, regardless of where they are born, who they are, or where they live". Through their life-saving vaccines, the research-based vaccine companies represented by th...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2012.12.032
更新日期:2013-04-18 00:00:00