Abstract：
:The effects of 0.1 to 500 mM ethanol on NMDA-activated currents were studied in primary cultures of mouse cortical and hippocampal neurons. In whole-cell recordings the IC50S for inhibition of NMDA-activated currents by ethanol were 129 mM +/- 20 mM in hippocampal neurons and 126 +/- 18 mM in cortical neurons. In single-channel recordings from excised outside-out patches of cortical neurons, ethanol inhibited total charge per minute with an IC50 of 174 +/- 23 mM, which was not significantly different from the IC50S for inhibition of whole-cell current. The reduction in mean open channel lifetime by ethanol was fit by the logistic equation with an apparent IC50 of 340 +/- 28 mM. Analysis of single-channel data indicated that ethanol inhibition of NMDA currents did not involve substantial changes in fast closed state kinetics, changes in open channel conductance, or block of the open channel. At the whole-cell IC50 of ethanol, mean open channel lifetime would decrease by 28% and frequency of opening would decline by 31% to account for the reduction in current. Single-channel data were consistent with ethanol being an allosteric modulator of gating which reduces agonist efficacy.
journal_name
Brain Resjournal_title
Brain researchauthors
Wright JM,Peoples RW,Weight FFdoi
10.1016/s0006-8993(96)00780-9subject
Has Abstractpub_date
1996-11-04 00:00:00pages
249-56issue
2eissn
0006-8993issn
1872-6240pii
S0006-8993(96)00780-9journal_volume
738pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The duration of the sensitive period of the kitten visual cortex to the effects of monocular deprivation was explored by studies of the behavioral and physiological recovery from extended periods of monocular occlusion imposed from birth, and by examination of the physiological effects of a 3 month period of monocular...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)91303-7
更新日期：1980-06-09 00:00:00
abstract：:Compelling evidence from experimental animal disease models and early-phase clinical trials identifies the transplantation of neural progenitor/stem cells (NSCs) as a viable path towards the development of clinically applicable exogenous stem cell therapies. Building from current advances in the field of NSC biology a...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2019.146615
更新日期：2020-02-15 00:00:00
abstract：:The ontogeny of muscarinic cholinergic receptors was studied in primary cultures of dissociated rat hippocampal cells. The specific [3H]quinuclidinyl benzilate [( 3H]QNB) to intact cells was detected after two days in culture. Scatchard analysis revealed only a unique binding site at all of the days tested, with a ver...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90019-8
更新日期：1987-09-01 00:00:00
abstract：:Several neurodevelopmental disorders, including schizophrenia, autism, ADD/ADHD and dyslexia are believed to originate during gestation and involve white matter abnormalities. Modulation of glutamate environments and glutamate receptors has also been implicated in alteration of oligodendrocytes, the myelin forming cel...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.06.126
更新日期：2008-10-09 00:00:00
abstract：:Entopeduncular nucleus (EPN) cells which project to thalamic and non-thalamic sites were identified by antidromic discharge. Each population of cells, projecting to the ventral anterior nucleus of the thalamus (VA), centrum medianum (CM), the lateral habenula (LHB), and the pedunculopontine nucleus (PP), was distribut...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90993-9
更新日期：1978-11-17 00:00:00
abstract：:The effect of an extended program of perforant path or amygdala kindling on paired-pulse suppression in the dentate gyrus was studied in male hooded rats. Repeated kindling stimulations were delivered twice or three times daily until either 300 stimuli had been delivered or generalized convulsions had been observed to...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)01277-o
更新日期：1995-01-23 00:00:00
abstract：:Adult human bone marrow stromal cells (hMSCs) grown in suspension culture gave rise to spheres of neural progenitor (NP) cells, capable of expressing both dopaminergic (DA) and GABAergic (GABA) traits. After transplantation into the Parkinsonian rat, human NPs and neurons were present at 2 weeks. Although no DA neuron...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.05.109
更新日期：2006-08-23 00:00:00
abstract：:The localization of vasoactive intestinal peptide (VIP)-like immunoreactivity was investigated in the brain, olfactory system and retina of the zebrafish, Danio rerio, during development and in juvenile specimens, by using the indirect immunofluorescence and the peroxidase-antiperoxidase methods. In 24 h post fertiliz...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03065-1
更新日期：2001-01-12 00:00:00
abstract：:The effects of two kinds of antibiotics, minocycline (MNC) and ampicillin (AB-PC), on human sleep were investigated on 19 healthy male students to test for a relationship between human sleep and protein synthesis. These drugs and placebos were capsulated identically in appearance and were given to the subjects using t...
journal_title：Brain research
pub_type： 临床试验,杂志文章
doi：10.1016/0006-8993(83)90101-4
更新日期：1983-12-12 00:00:00
abstract：:The regenerating optic nerve of goldfish first reestablishes a rough retinotopic map on the tectum, then goes through an activity dependent refinement that appears to involve the elimination of inappropriate branches from early regenerated arbors. Retinotopically appropriate branches and synapses may be stabilized bec...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90886-4
更新日期：1988-04-19 00:00:00
abstract：:Following cholinergic denervation of the hippocampus by medial septal lesions, and unusual neuronal reorganization occurs, in which peripheral sympathetic fibers, originating from the superior cervical ganglia, grow into the hippocampus. Previously, we have found that both hippocampal sympathetic ingrowth (HSI) and ch...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00070-7
更新日期：1995-04-10 00:00:00
abstract：:We have attempted to identify some novel genes which were found to be more highly expressed in the embryonic brain than in the adult brain. Consequently, one of these clones was identified as alpha-globin cDNA. Actually, alpha-globin mRNA was detected in the neurons. In addition, beta-globin mRNA was detected in the n...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91455-9
更新日期：1994-01-28 00:00:00
abstract：:The effects of kindling induced in developing rat pups were re-examined when the rats reached maturity. Eighteen-day-old rats, implanted with bipolar electrodes, were separated into the following 4 groups: the fully kindled group consisted of pups that developed generalized convulsions; the partially kindled group of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(82)90097-9
更新日期：1982-05-01 00:00:00
abstract：:We have conducted studies in intact adult male rats, designed to examine the effect of a 3- or 7-day exposure to alcohol (EtOH) on the pituitary's response to corticotropin-releasing hormone (CRF) or stress, and on CRF expression in the hypothalamus. In a first series of experiments, rats exposed to EtOH vapors for 7 ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91685-a
更新日期：1990-06-18 00:00:00
abstract：:The neurochemical heterogeneity of the rat striatum was assessed in vivo by measuring subregional changes in extracellular dopamine and DOPAC by in vivo electrochemistry and microdialysis in response to amphetamine and the D2 antagonist, (-)-sulpiride. Both in vivo electrochemical and microdialysis experiments indicat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91256-g
更新日期：1990-01-08 00:00:00
abstract：:(1) Input conductances of giant interneurons in the isolated spinal cord of lampreys were measured with two separate intracellular electrodes. Bath applications of 0.4--3 mM beta-alanine and taurine produced large, reversible conductance increases which were Cl-dependent. (2) Strychnine at 1--2.5 microM might be a com...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90628-0
更新日期：1979-09-14 00:00:00
abstract：OBJECTIVE:Recent research has shown dissociation between changes in brain and muscle signals during voluntary muscle fatigue, which may suggest weakening of functional corticomuscular coupling. However, this weakening of brain-muscle coupling has never been directly evaluated. The purpose of this study was to address t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.10.074
更新日期：2009-01-23 00:00:00
abstract：:Local formation of oestrogens from androgens by aromatase cytochrome P-450 within brain cells is crucial for the sexual differentiation of the mammalian CNS. Aromatase activity has been detected in several brain regions of the developing rodent brain. In the present study, we used a mouse-specific, peptide-generated, ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90651-3
更新日期：1994-02-28 00:00:00
abstract：:The parafascicular thalamic nucleus (nPf) is a critical relay in the ascending system that mediates motor control in the central nervous system (CNS). Yet, little is known about whether or not the nPf is involved in the development of morphine dependence and withdrawal. In the present study, kainic acid was used to ch...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.02.084
更新日期：2009-05-19 00:00:00
abstract：:While exercise is commonly recommended for PD patients to improve motor function, little is known about the disease-altering potential of exercise. Although others have demonstrated neuroprotective or neurorestorative effects of exercise in animal models of PD, the majority of these studies utilize young animals. In o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.06.032
更新日期：2016-09-01 00:00:00
abstract：:Pregabalin is useful for treating neuropathic pain, but known to increase body weight as a side effect. To investigate the mechanism of this increase in body weight, we focused on dopamine in the lateral hypothalamus (LH) and examined the effects of pregabalin on dopamine levels in the LH and food intake. The dopamine...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.09.026
更新日期：2018-12-15 00:00:00
abstract：:Astrocytic gap junctions have been implicated in a variety of signaling pathways essential to normal brain function. However, no information exists on the prevalence of gap junction channels and their function in the aging brain. Here we have compared the expression of the two most abundant astrocytic gap junction pro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02258-2
更新日期：2001-05-18 00:00:00
abstract：:Initial studies found glutamate injury to murine spinal cultures (14-17 days in vitro) to reflect contributions of both NMDA and AMPA/kainate receptors. Subsequent experiments found the spinal cultures to be more sensitive than cortical cultures to injury from prolonged low level kainate exposures, and, unlike cortica...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00532-u
更新日期：1995-08-21 00:00:00
abstract：:The development of symptomatic epilepsy is a model of long-term plasticity changes in the central nervous system. The rat pilocarpine model of epilepsy was utilized to study persistent alterations in calcium/calmodulin-dependent kinase II (CaM kinase II) activity associated with epileptogenesis. CaM kinase II-dependen...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02623-8
更新日期：2000-09-01 00:00:00
abstract：:The effect of high acetaldehyde (ACe) on acetylcholine (ACh) release was studied in vivo in the medial frontal cortex (mfc) of freely moving rats using brain microdialysis coupled with high performance liquid chromatography and an electrochemical detector. Ethanol (EtOH) and ACe concentrations were quantified simultan...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.01.049
更新日期：2005-03-28 00:00:00
abstract：:Visual search is characterized as efficient (RT independent of distractor number) or inefficient (RT increasing with distractor number). Our goal was to determine if any brain regions are differentially activated by the attentional demands of these two search modes. We used fMRI to examine activation patterns during s...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.07.004
更新日期：2012-09-26 00:00:00
abstract：:Monkeys can gradually increase or decrease the amplitude of the segmentally mediated spinal stretch reflex (SSR) without change in initial muscle length or background EMG activity. Both increase (under the SSR increases mode) and decrease (under the SSR decreases mode) occur slowly, progressing steadily over weeks. Th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90259-7
更新日期：1983-11-14 00:00:00
abstract：:This study determined whether macrophage inflammatory protein-1beta (MIP-1beta) plays a role in the hyperthermia caused by prostaglandin E2 (PGE2) given intracerebroventricularly (i.c.v.) in the rat. In these experiments, anti-murine MIP-1beta antibody (anti-MIP-1beta) was micro-injected in the anterior hypothalamic, ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01362-5
更新日期：1997-02-14 00:00:00
abstract：:The modulation of tetrodotoxin-sensitive (TTX-S) and slow tetrodotoxin-resistant (TTX-R) sodium currents in rat dorsal root ganglion neurons by ATP was studied using the whole-cell patch-clamp method. The effects of ATP on two types of sodium currents were either stimulatory or inhibitory depending on the kinetic para...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)04218-x
更新日期：2003-04-04 00:00:00
abstract：BACKGROUND:It is unclear whether spinal cord stimulation (SCS) at higher frequencies induces further increases in vasodilation and enhances clinical efficacy. OBJECTIVES:This study investigated effects of SCS at both a normal frequency (as used clinically) and two higher frequencies on peripheral vasodilation. METHOD...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.11.072
更新日期：2010-02-08 00:00:00
