Abstract：
:The PII protein has been considered pivotal to the dual cascade regulating ammonia assimilation through glutamine synthetase activity. Here we show that PII, encoded by the glnB gene, is not always essential; for instance upon ammonia deprivation of a glnB deletion strain, glutamine synthetase can be deadenylylated as effectively as in the wild-type strain. We describe a new operon, glnK amtB, which encodes a homologue of PII and a putative ammonia transporter. We cloned and overexpressed glnK and found that the expressed protein had almost the same molecular weight as PII, reacted with polyclonal PII antibody, and was 67% identical in terms of amino acid sequence with Escherichia coli PII. Like PII, purified GlnK can activate the adenylylation of glutamine synthetase in vitro, and, in vivo, the GlnK protein is uridylylated in a glnD-dependent fashion. Unlike PII, however, the expression of glnK depends on the presence of UTase, nitrogen regulator I (NRI), and absence of ammonia. Because of a NRI and a sigma N (sigma 54) RNA polymerase-binding consensus sequence upstream from the glnK gene, this suggests that glnK is regulated through the NRI/NRII two-component regulatory system. Indeed, in cells grown in the presence of ammonia, glutamine synthetase deadenylylation upon ammonia depletion depended on PII. Possible regulatory implications of this conditional redundancy of PII are discussed.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
van Heeswijk WC,Hoving S,Molenaar D,Stegeman B,Kahn D,Westerhoff HVdoi
10.1046/j.1365-2958.1996.6281349.xsubject
Has Abstractpub_date
1996-07-01 00:00:00pages
133-46issue
1eissn
0950-382Xissn
1365-2958journal_volume
21pub_type
杂志文章abstract：:NADPH oxidases (Nox) produce reactive oxygen species (ROS) in multicellular eukaryotic organisms. They trigger defense reactions ('oxidative burst') - in phagocytes and plant cells -, and are involved in a broad range of differentiation processes. Fungal Nox-complexes play a central role in vegetative, sexual and path...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13391
更新日期：2016-07-01 00:00:00
abstract：:Computer analysis of the crystallographic structure of the A subunit of Escherichia coli heat-labile toxin (LT) was used to predict residues involved in NAD binding, catalysis and toxicity. Following site-directed mutagenesis, the mutants obtained could be divided into three groups. The first group contained fully ass...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb01266.x
更新日期：1994-10-01 00:00:00
abstract：:Helicobacter pylori is a human gastric pathogen that colonizes ∼ 50% of the world's population. It can cause gastritis, gastric or duodenal ulcers and also gastric cancer. The numerous side effects of available treatments and the emergence of antibiotic resistant strains are severe concerns that justify further resear...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12923
更新日期：2015-04-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01620.x
更新日期：1993-05-01 00:00:00
abstract：:Pseudomonas aeruginosa, an opportunistic human pathogen, is a major causative agent of mortality and morbidity in immunocompromised individuals and those with cystic fibrosis (CF). In CF patients, the secretion of abnormally high amounts of mucus into the airways contributes to their susceptibility to infection by P. ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1996.01533.x
更新日期：1996-12-01 00:00:00
abstract：:Regulation of polar development and cell division in Caulobacter crescentus relies on the dynamic localization of several proteins to cell poles at specific stages of the cell cycle. The polar organelle development protein, PodJ, is required for the synthesis of the adhesive holdfast and pili. Here we show the cell cy...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03349.x
更新日期：2003-02-01 00:00:00
abstract：:Conjugative transfer of toxin and antibiotic resistance plasmids in Clostridium perfringens is mediated by the tcp conjugation locus. Surprisingly, neither a relaxase gene nor an origin of transfer (oriT) has been identified on these plasmids, which are typified by the 47 kb tetracycline resistance plasmid pCW3. The t...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13270
更新日期：2016-03-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04746.x
更新日期：2005-08-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.14111
更新日期：2018-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01611.x
更新日期：1993-05-01 00:00:00
abstract：:Until recently, rare-earth elements (REEs) had been thought to be biologically inactive. This view changed with the discovery of the methanol dehydrogenase XoxF that strictly relies on REEs for its activity. Some methylotrophs only contain xoxF, while others, including the model phyllosphere colonizer Methylobacterium...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.14208
更新日期：2019-05-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12863
更新日期：2015-01-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1998.00974.x
更新日期：1998-08-01 00:00:00
abstract：:Bacterial cytokinesis is orchestrated by an assembly of essential cell division proteins that form a supramolecular structure known as the divisome. DivIB and its orthologue FtsQ are essential members of the divisome in Gram-positive and Gram-negative bacteria respectively. DivIB is a bitopic membrane protein composed...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06114.x
更新日期：2008-03-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.05739.x
更新日期：2007-06-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.1993.tb01702.x
更新日期：1993-08-01 00:00:00
abstract：:The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1989.tb00228.x
更新日期：1989-06-01 00:00:00
abstract：:Spatio-temporal oscillations of the Min proteins are essential for selecting the cell division site in Escherichia coli. These oscillations are a key example of a biological phenomenon that can only be understood on a systems level rather than on the level of its individual components. Here, we review the key concepts...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.2007.05607.x
更新日期：2007-03-01 00:00:00
abstract：:Mycobacterium smegmatis mc2 155 has three genes (MSMEG_6383, furA1; MSMEG_3460, furA2; MSMEG_6253, furA3) encoding FurA (ferric-uptake regulator A) paralogs. Three FurA paralogs in M. smegmatis are functionally redundant and negatively regulate expression of a subset of genes involved in peroxide detoxification such a...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13956
更新日期：2018-06-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2011-08-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb02177.x
更新日期：1994-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1988.tb00088.x
更新日期：1988-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2958.1998.00978.x
更新日期：1998-08-01 00:00:00
abstract：:Salmonella enterica subspecies I serotypes are responsible for the vast majority of salmonellosis in mammals and birds, yet only a few factors specific to this group that allow them to persist in this niche have been identified. We show that STM0557, a S. enterica subspecies I-specific gene encoding an inner membrane ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06461.x
更新日期：2008-12-01 00:00:00
abstract：:While examining the molecular basis for the lack of actin-based motility for the non-pathogenic spotted fever group (SFG) R. peacockii, we identified a novel insertion sequence (IS) element, ISRpe1, disrupting the coding sequence of rickA, demonstrated to induce actin-tail polymerization for the SFG rickettsiae. This ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04806.x
更新日期：2005-10-01 00:00:00
abstract：:mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge. Sequences more or less similar to mscL are found in other bacteria, but the degree of conserved function has been unclear. We subcloned and expressed these putative homologues in E. coli and exami...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1998.00821.x
更新日期：1998-05-01 00:00:00
abstract：:Cellulosomes are key for lignocellulosic biomass degradation in cellulolytic Clostridia. Better understanding of the mechanism of cellulosome regulation would allow us to improve lignocellulose hydrolysis. It is hypothesized that cellulosomal protease inhibitors would regulate cellulosome architecture and then lignoce...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12488
更新日期：2014-02-01 00:00:00
abstract：:In matings between Lactococcus lactis strains, the conjugative transposons Tn916 and Tn919 are found in the chromosome of the transconjugants in the same place as in the chromosome of the donor, indicating that no transposition has occurred. In agreement with this, the frequency of L. lactis transconjugants from intra...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1991.tb01858.x
更新日期：1991-12-01 00:00:00
abstract：:Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.05984.x
更新日期：2007-12-01 00:00:00
