Abstract：
:T cells in vivo have been shown to accumulate DNA damage with age. To investigate the effects of DNA damage on T cell biology we have utilised an in vitro human CD4+ T cell clone model. Levels and types of DNA damage were determined in 11 independent T cell clones as a function of their in vitro lifespan. Increased levels of reactive oxygen species (ROS) induced DNA damage with increasing age were found in all clones analysed using a modified alkaline comet assay. T cell clones underwent apoptosis at the end of their lifespans. There were no consistent changes in the mRNA levels for the cyclin-dependent kinase inhibitors (CKI) p16, p21, and p27 during the clones' lifespans. It appears that the increased levels of ROS induced DNA damage in the T cells is not the major trigger of apoptosis, via the p53/p21 pathway. In addition, at the end of their lifespans, the T cell clones did not display the CKI phenotype reported for senescent cells (an increase in p16 and p21 levels). Thus, while the T cell clones appear sensitive to ROS-induced DNA damage, the molecular mechanisms through which this influences T cell dysfunction with age remains to be elucidated.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Hyland P,Barnett C,Pawelec G,Barnett Ydoi
10.1016/s0047-6374(01)00254-8subject
Has Abstractpub_date
2001-08-01 00:00:00pages
1151-67issue
11eissn
0047-6374issn
1872-6216pii
S0047-6374(01)00254-8journal_volume
122pub_type
杂志文章abstract：:Interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) concentrations were measured in livers of young-adult and old rats administered carbon tetrachloride or vehicle. IL-1beta levels were higher and IL-6 levels were lower in old rats than in young-adult rats. Carbon tetrachlor...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(99)00012-3
更新日期：1999-05-03 00:00:00
abstract：:Because muscle satellite cells have been implicated in the process of muscle growth and mass regulation, as well as regeneration, alterations in the capacity of satellite cells to differentiate and accumulate muscle specific proteins during aging could play a role in the process of senile muscle atrophy. Skeletal musc...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(82)90033-1
更新日期：1982-01-01 00:00:00
abstract：:The effects of age on estrogen stimulated nuclear RNA polymerase II activity were studied in uteri of ovariectomized mature (6-8 months) and senescent (24 months) rats. Basal levels of RNA polymerase II activity were the same in both age groups. Following 17 beta-estradiol (E2) administration, RNA polymerase II activi...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(84)90136-2
更新日期：1984-04-01 00:00:00
abstract：:A few reports suggest that pigmented granules found in molluscan neurons accumulate with age as do lipofuscin granules in vertebrate cells; however, no reports on molluscan neurons include detailed descriptions of granule accumulation or histochemical tests to identify the pigment as lipofuscin-like. In this study lig...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(78)90052-0
更新日期：1978-01-01 00:00:00
abstract：:Hepatocyte morphology of the three zones of the liver lobule was evaluated by stereological analysis in 3-month-old and 35-month-old virgin female WAG/Rij rats. In the old rats, the volume of the nucleus and cytoplasm, and volume densities of smooth endoplasmic reticulum and lysosomes were increased in all areas of th...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(80)90054-8
更新日期：1980-06-01 00:00:00
abstract：:The age changes of neural control over the function of the thyroid, adrenal cortex and testicles were examined in adult (6 months) and old (28 months) male rats. In old age, there was a weakening of adrenergic control of thyroidogenesis, alpha-adrenergic and M-cholinergic control of glucocorticoid function of the adre...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(90)90165-c
更新日期：1990-01-01 00:00:00
abstract：:Age-specific mortality from carcinoma of the prostate was analyzed by drawing a line between the value at age 52 years and that at age 87. Slope of the line was relatively constant for the pooled years 1962-1966, 1967-1971 and 1972-1976 (maximum variation from the mean of 4.5%). However, the slope was 17.7% greater fo...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(95)01608-3
更新日期：1995-08-31 00:00:00
abstract：:A progressive decrease of dopamine and related enzyme activities and a decline in the dopaminergic receptor functions in the striatum and limbic system controlling behavior have been observed in senescent brain. These changes provide a neurochemical basis for some behavioral and mental disturbances of the aged and sug...
journal_title：Mechanisms of ageing and development
pub_type： 临床试验,杂志文章
doi：10.1016/0047-6374(80)90125-6
更新日期：1980-09-01 00:00:00
abstract：:The process of epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) after cataract surgery contributes to tissue fibrosis, wound healing and lens regeneration via a mechanism not yet fully understood. Here, we show that tropomyosin 2 (Tpm2) plays a critical role in wound healing and lens aging. Post...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2018.03.001
更新日期：2018-04-01 00:00:00
abstract：:Susceptibility for Friend leukemia virus (FLV)-induced leukemogenesis was examined in the C3H/He (C3H)-->C57BL/6 (B6) radiation bone marrow chimeras of various age groups, and the effect of aging of host mice on the susceptibility was determined. The bone marrow chimera system provided the various age of FLV-resistant...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(00)00245-1
更新日期：2001-02-01 00:00:00
abstract：:The current increase in life expectancy observed in industrialized societies underscores the need to achieve a better understanding of the aging process that could help the development of effective strategies to achieve healthy aging. This will require not only identifying genes involved in the aging process, but also...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2006.12.003
更新日期：2007-03-01 00:00:00
abstract：OBJECTIVES:To investigate whether systemic inflammation in acutely admitted older medical patients (age >65 years) is associated with physical performance and organ dysfunction. Organ dysfunction´s association with physical performance, and whether these associations are mediated by systemic inflammation, was also inve...
journal_title：Mechanisms of ageing and development
pub_type： 临床试验,杂志文章
doi：10.1016/j.mad.2017.04.005
更新日期：2017-06-01 00:00:00
abstract：:The serotonin content of platelets, serum and plasma from rats of various ages was examined. In male rats, platelet serotonin content, which was about 0.65 nmol/10(8) platelets at young age (6-7 months), increased slightly at middle age (12-14 months) but decreased markedly at old age (25-26 months). Significant diffe...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(89)90008-0
更新日期：1989-01-01 00:00:00
abstract：:Survival characteristics of seven different populations of the mollusc Lymnaea stagnalis were studied under constant culture conditions. On the basis of these characteristics four populations were considered as healthy and three as infected. In the healthy populations senescence started at an age of about 200 days, 50...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(88)90052-8
更新日期：1988-03-01 00:00:00
abstract：:The present work explores the application of information theoretical measures, such as entropy and normalized mutual information, for research of biomarkers of aging. The use of information theory affords unique methodological advantages for the study of aging processes, as it allows evaluating non-linear relations be...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2017.01.003
更新日期：2017-04-01 00:00:00
abstract：:To examine the effect of an aging environment on sister chromatid exchange (SCE) induction, Ehrlich ascites tumor (EAT) cells were introduced into young and old C57BL/6J mice. Background SCE levels were not significantly different in either EAT cell or normal bone marrow cell populations between young and old animals....
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(81)90051-8
更新日期：1981-12-01 00:00:00
abstract：:Mice with an overall deletion of the sensory neuropeptide α-calcitonin gene-related peptide (α-CGRP) develop an age-dependent osteopenic bone phenotype. Underlying molecular mechanisms of how αCGRP affects bone cell metabolism are not well understood. This study aims to characterize differences in metabolic parameters...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2020.111265
更新日期：2020-07-01 00:00:00
abstract：:This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of Marie-Ange Teste, Isabelle Léger-Silvestre, Jean M François and Jean-Luc Parrou. Marjorie Petitjean could not b...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,收录出版
doi：10.1016/j.mad.2016.07.011
更新日期：2017-01-01 00:00:00
abstract：:DNA-repair capacity, by incorporation in vitro of [3H]thymidine into DNA of isolated neuronal cells and splenic lymphocytes of rat was studied as a function of age. The incubations were carried out both in the presence and absence of hydroxyurea (HU), a known inhibitor of replicative DNA synthesis. The results indicat...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(91)90053-3
更新日期：1991-03-01 00:00:00
abstract：:Normal diploid Syrian hamster dermal mesenchymal cell strains, regardless of the age of the tissue of origin, exhibit in vitro cellular senescence. The frequency of spontaneous escape from senescence and conversion to a permanent cell line is less than 5% among replicate flasks. The overall pattern of senescence of ce...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(86)90032-1
更新日期：1986-04-01 00:00:00
abstract：:Motor neurons and ventral root motor fibres innervating extensor digitorum longus (EDL) muscle in rats of 0.5-3.5 months of age were studied by HRP-retrograde labelling and in vivo muscle tension recording, respectively. EDL nucleus size increased with age but motor neurons number and size did not change. Twitch and t...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(96)01839-8
更新日期：1996-12-20 00:00:00
abstract：:In this paper, we approach the problem of attempting to understand senescence by understanding the complexity and hierarchical structure of mammalian organisms. To do this, we make use of some concepts from abstract relational biology; in particular, graph theory. We subsequently utilize these concepts to develop the ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(84)90056-3
更新日期：1984-10-31 00:00:00
abstract：:The conserved SIR2 protein regulates life span in both yeast and worms: in both organisms overexpression of SIR2 can extend life span and in Caenorhabditis elegans this life span extension is dependent on the forkhead transcription factor, DAF-16. Here, we have done extensive genetic analysis with sir-2.1(ok434), a nu...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2005.09.005
更新日期：2006-01-01 00:00:00
abstract：:The risk for developing colorectal cancer increases exponentially with age. We demonstrate that spontaneous loss of folate in the colon results in DNA damage accumulation and aberrant DNA damage responses that may contribute to the increased genomic instability and cancer risk in colon. We find greater than 2-fold cha...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2012.01.003
更新日期：2012-02-01 00:00:00
abstract：:Replicative senescence in human diploid fibroblasts is characterised by an exhaustion of proliferative potential and permanent cell cycle arrest. During senescence, telomere shortening-generated DNA damage activates p53 pathway that upregulates cell cycle inhibitors, such as p21. Human p400 ATPase is a chromatin remod...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2012.11.001
更新日期：2012-11-01 00:00:00
abstract：:At least five different transgenic approaches have been applied to the study of Drosophila aging. There are two single component systems: transgenes with native (normal) promoters and transgenes with heterologous promoters; as well as three binary systems: 'GAL4/UAS', 'FLP-out' and 'tet-on'. These approaches vary in a...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/s0047-6374(00)00152-4
更新日期：2000-09-01 00:00:00
abstract：:The health status of the oldest old, the fastest increasing population segment worldwide, progressively becomes more heterogeneous, and this peculiarity represents a major obstacle to their classification. We compared the effectiveness of four previously proposed criteria (Franceschi et al., 2000; Evert et al., 2003; ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2013.11.001
更新日期：2013-11-01 00:00:00
abstract：:There are multiple lines of evidence showing that oxidative stress and aberrant mitogenic signaling play an important role in the pathogenesis of Alzheimer disease. However, the chronological relationship between these and other events associated with disease pathogenesis is not known. Given the important role that mi...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(01)00342-6
更新日期：2001-12-01 00:00:00
abstract：:Great importance is attached to structural and functional deterioration of mitochondria as a reason for ageing of an organism; the attention of many scientists has been concentrated on such questions as age changes in the system of oxidative phosphorylation, damage of mitochondrial DNA by free radicals generated in th...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(93)90153-i
更新日期：1993-05-01 00:00:00
abstract：:Anucleate minicells produced by a mutated strain of Escherichia coli remain metabolically active for up to 48 h at 37 degrees C. Minicells of increasing age have been infected with the coliphage T7. Infection results in the onset of transcription and translation producing T7 encoded polypeptides. Quantitative and qual...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(84)90045-9
更新日期：1984-10-15 00:00:00
