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Candidate genes affecting Drosophila life span identified by integrating microarray gene expression analysis and QTL mapping.

Abstract：

:The current increase in life expectancy observed in industrialized societies underscores the need to achieve a better understanding of the aging process that could help the development of effective strategies to achieve healthy aging. This will require not only identifying genes involved in the aging process, but also understanding how their effects are modulated by environmental factors, such as dietary intake and life style. Although the human genome has been sequenced, it may be impractical to study humans or other long-lived organisms to gain a mechanistic understanding about the aging process. Thus, short-lived animal models are essential to identifying the mechanisms and genes that affect the rate and quality of aging as a first step towards identifying genetic variants in humans. In this study, we investigated gene expression changes between two strains of Drosophila (Oregon and 2b) for which quantitative trait loci (QTLs) affecting life span were identified previously. We collected males and females from both strains at young and old ages, and assessed whole genome variation in transcript abundance using Affymetrix GeneChips. We observed 8217 probe sets with detectable transcripts. A total of 2371 probe sets, representing 2220 genes, exhibited significant changes in transcript abundance with age; and 839 probe sets were differentially expressed between Oregon and 2b. We focused on the 359 probe sets (representing 354 genes) that exhibited significant changes in gene expression both with age and between strains. We used these genes to integrate the analysis of microarray gene expression data, bioinformatics, and the results of genetic mapping studies reported previously, to identify 49 candidate genes and four pathways that could potentially be responsible for regulating life span and involved in the process of aging in Drosophila and humans.

journal_name

Mech Ageing Dev

journal_title

Mechanisms of ageing and development

authors

Lai CQ,Parnell LD,Lyman RF,Ordovas JM,Mackay TF

doi

10.1016/j.mad.2006.12.003

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

237-49

issue

eissn

0047-6374

issn

1872-6216

pii

S0047-6374(06)00280-6

journal_volume

128

pub_type

杂志文章

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Candidate genes affecting Drosophila life span identified by integrating microarray gene expression analysis and QTL mapping.