Abstract:
:Glycosaminoglycan (GAG) distribution has been analyzed in the adhesion sites, left substratum-bound after EGTA-mediated detachment, of various human skin fibroblast populations grown in vitro in the presence (asc+) or absence (asc-) of ascorbate. Examination of these skin fibroblasts during the EGTA treatment by scanning electron microscopy reveals that (a) asc+ cells detach much more rapidly than asc- cells, but (b) asc- or asc+ cells leave the same two types of structures in longterm culture-generated substratum-attached material (L-SAM)--long linear retraction fibers and "footpad-like" structures. Most of the [3H]glucosamine-radiolabeled polysaccharides in L-SAM were shown to be GAGs. Fibroblasts from a full-thickness skin sample from a very young patient (AG4449) have similar distributions of the GAGs in both the EGTA-suspended cell and L-SAM fractions; however, asc+ cell and L-SAM fractions contain relatively more heparan sulfate than the asc- fractions. In contrast, full-thickness skin fibroblasts from an elderly patient (AG2261) generate GAG distributions in their L-SAMs (with greatly elevated levels of hyaluronate and chondroitin sulfate) that are very different from those of the cell fractions and from those of AG4449; furthermore, these distributions in AG2261 fractions do not change when shifted from asc- to asc+ medium. These studies led to analyses of the two major fibroblast subsets--papillary (PAP) or reticular (RET)--that can be isolated from the dermis of a newborn infant (patient 5). The GAG distributions in the RET fractions were different from those in PAP fractions; of special note was the greater length of heparan sulfate chains from all RET fractions examined when compared to PAP fractions. There was a remarkable similarity in the GAG distributions of asc+ RET fractions when compared to the full-thickness AG2261 cell fractions. In summary, these studies demonstrate that asc- or asc+ "young" cells generate different GAG distributions in their substratum adhesion sites, whereas "old" cells from a full-thickness skin sample do not alter their distribution when shifted from asc- to asc+ (this distribution is different from that of "young" cells). Furthermore, analyses of GAGs in papillary and reticular cell fractions reveal significant differences between the two, with considerable similarity of asc+ reticular fractions to the full-thickness AG2261 fibroblasts, which is consistent with the enrichment of reticular fibroblasts in the skin of aging individuals.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Beyth RJ,Culp LAdoi
10.1016/0047-6374(85)90015-6subject
Has Abstractpub_date
1985-02-01 00:00:00pages
151-69issue
2eissn
0047-6374issn
1872-6216pii
0047-6374(85)90015-6journal_volume
29pub_type
杂志文章abstract::Lipid peroxidation, one of the most common expressions of oxidative stress, may be altered during normal physiological states including development. We have examined the changes in lipid peroxidation in rat brain, a tissue highly susceptible to oxidative damage, during this physiological state. In vivo lipid peroxidat...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)90052-3
更新日期:1994-03-01 00:00:00
abstract::A nuclease that releases noncomplementary nucleotides from the 3'-end of DNA was isolated and highly purified from rat liver extract. The d(T9-C) priming activities for DNA synthesis in vitro by DNA polymerases alpha and beta were recovered by the addition of this enzyme, which itself does not contain a DNA polymerase...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(97)00114-0
更新日期:1998-01-12 00:00:00
abstract::Aging causes changes in body composition and energy balance. Estrogen plays an important role in body's metabolism. The aim of this study was to determine whether estrogen has beneficial effects on leptin responsiveness in aged mice. Young 4 months and aged 19-21 female mice fed High Fat Diet (HFD) or Standard Diet (S...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2019.111198
更新日期:2020-03-01 00:00:00
abstract::The potential gain in life expectancy which could result from the complete elimination of mortality from cancer in the U.S. would not exceed 3 years if one were to consider cancer independently of other causes of death. In this paper, we review evidence of trade-offs between cancer and aging as well as between cancer ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2008.03.006
更新日期:2009-01-01 00:00:00
abstract::Fluorescent microspheres were used to measure antibody-induced capping of leukocyte membrane proteins that are immunologically related to band 3, the anion exchanger of the erythrocyte. The degree of capping was found to increase with donor age. Surface labeling and capping characteristics of cells from healthy, age-m...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)01553-x
更新日期:1995-04-21 00:00:00
abstract::In this paper, we approach the problem of attempting to understand senescence by understanding the complexity and hierarchical structure of mammalian organisms. To do this, we make use of some concepts from abstract relational biology; in particular, graph theory. We subsequently utilize these concepts to develop the ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(84)90056-3
更新日期:1984-10-31 00:00:00
abstract::A quantitative ultrastructural study of the posterior pituitary was undertaken. The posterior pituitary is the storage of the hormones vasopressin and oxytocin. Female C57BL/Icrfat mice which were under conditions of normal hydration were sampled at 8 and 32 months of age. There was a slight age-related decline in the...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(81)90069-5
更新日期:1981-02-01 00:00:00
abstract::Many molecular chaperones are also known as heat shock proteins because they are synthesised in increased amounts after brief exposure of cells to elevated temperatures. They have many cellular functions and are involved in the folding of nascent proteins, the re-folding of denatured proteins, the prevention of protei...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2004.09.031
更新日期:2005-01-01 00:00:00
abstract::The strychnine-insensitive binding of [3H]glycine was characterized in purified cerebral cortical membranes from mice aged from 7 days to 22 months. The binding was saturable, exhibiting only one component during the whole life-span studied. The binding constant KD did not change during development and ageing, whereas...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(93)90131-a
更新日期:1993-11-01 00:00:00
abstract::Active oxygen species have been considered to be responsible for the aging process and for the induction and initiation of neoplastic processes. The effect of hydrogen peroxide, an active oxygen species, was investigated in the chromosomes of three young women (20-21 years of age) and of three elderly women (73-79 yea...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(90)90002-w
更新日期:1990-11-01 00:00:00
abstract::Because muscle satellite cells have been implicated in the process of muscle growth and mass regulation, as well as regeneration, alterations in the capacity of satellite cells to differentiate and accumulate muscle specific proteins during aging could play a role in the process of senile muscle atrophy. Skeletal musc...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(82)90033-1
更新日期:1982-01-01 00:00:00
abstract::In yeast, increasing the copy number of the nicotinamide adenine dinucleotide (NAD)-dependent deacetylase Sir2 extends lifespan, which can be inhibited by nicotinamide (Nam), the end-product of Sir2-mediated NAD-breakdown. Furthermore, the yeast pyrazinamidase/nicotinamidase PNC-1 can extend yeast lifespan by converti...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2007.01.004
更新日期:2007-04-01 00:00:00
abstract::Ageing is a complex multifactorial process that results in many changes in physiological changes processes that ultimately increase susceptibility to a wide range of diseases. As such an ageing population is resulting in a pressing need for more and improved treatments across an assortment of diseases. Such treatments...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2016.07.005
更新日期:2016-12-01 00:00:00
abstract::Chromosome analyses and Ig level determinations were carried out on seventeen aged men and women, 83--100 years of age. In men, significant correlations were found between hypodiploidy and IgA (gamma = 0.95) and between monosomy C and IgG (gamma = 0.79) while in women, monosomy G and IgG were significantly correlated ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(78)90039-8
更新日期:1978-12-01 00:00:00
abstract::Specific T-cell cytotoxic responses to allogeneic and hapten-modified syngeneic cells decrease with age. In order to determine the causes of these reduced T-cell cytotoxic responses, spleen cells from individual young and senescent C57BL/6J female mice were mixed in various proportions in culture with either X-irradia...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(84)90135-0
更新日期:1984-04-01 00:00:00
abstract::The presence of damaged and microbial DNA can pose a threat to the survival of organisms. Cells express various sensors that recognize specific aspects of such potentially dangerous DNA. Recognition of damaged or microbial DNA by sensors induces cellular processes that are important for DNA repair and inflammation. He...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2016.09.001
更新日期:2017-07-01 00:00:00
abstract::Components of the calmodulin system (i.e., calmodulin levels and activities of the following calmodulin-dependent enzymes: Ca2+ + Mg2+-ATPase, adenylate and guanylate cyclases, cyclic AMP and cyclic GMP phosphodiesterases, and Ca2+-dependent protein kinase were studied in the following brain regions from immature (25-...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(86)90018-7
更新日期:1986-10-01 00:00:00
abstract::Elevated reactive oxygen species (ROS) levels have been observed in mammals during aging, implying an important role of ROS in the aging process. Most bird species are known to live longer and to contain lower ROS levels than mammals of the same body weight. The influence of ROS on the aging process of birds has been ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2009.11.005
更新日期:2010-01-01 00:00:00
abstract::Semantic distinctions between "normal" aging, "pathological" aging (or age-related disease) and "premature" aging (otherwise known as segmental progeria) potentially confound important insights into the nature of each of the complex processes. Here we review a recent, unexpected discovery: the presence of longevity-as...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2006.11.011
更新日期:2007-01-01 00:00:00
abstract::To test the validity of estimating age-related changes in rat liver nuclear RNA synthesis by following the incorporation of radioactive nucleotides into an acid-insoluble product, age-related changes in endogenous nucleotide concentration were investigated. Both the total nucleotide and the nucleoside monophosphate (N...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(80)90087-1
更新日期:1980-02-01 00:00:00
abstract::In this review we discuss the yeast as a paradigm for the study of aging. The budding yeast Saccharomyces cerevisiae, which can proliferate in both haploid and diploid states, has been used extensively in aging research. The budding yeast divides asymmetrically to form a 'mother' cell and a bud. Two major approaches, ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/s0047-6374(00)00182-2
更新日期:2000-12-01 00:00:00
abstract::The survival rate was measured of differently aged rabbit erythrocytes that had been taken from young and old animals, tagged with 51Cr and injected into young and old animals. The survival rate of the donor erythrocytes depends mostly on the age of the recipient animals. The life span of the injected cells found in o...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(81)90063-4
更新日期:1981-11-01 00:00:00
abstract::The present study investigated whether age-related changes in the production of Th1 and Th2 cytokines by human T cells might be linked to altered frequencies of naive (CD45RA+) and memory (CD45RO+) T cell subsets. T cells from healthy elderly humans (n = 32) stimulated with anti-CD3epsilon monoclonal antibody OKT3 plu...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(99)00030-5
更新日期:1999-08-30 00:00:00
abstract::The effect of age on potassium (K+)-induced norepinephrine release in the heart was studied in 6- and 24-month-old male Fischer-344 rats. Isolated hearts were perfused with Krebs-Ringer solution according to Langendorff method. K+ was employed as 2 ml bolus at the concentrations, of 70, 90, 100 and 120 microM. Norepin...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(90)90147-8
更新日期:1990-03-01 00:00:00
abstract::The peripheral-type benzodiazepine receptor (PBR) is a protein predominantly located in the mitochondrial outer membrane that plays an important role in the regulation of cell survival and proliferation. Previous studies have shown an enhanced expression of PBR in the regenerating sciatic nerve, suggesting that this p...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2005.06.001
更新日期:2005-11-01 00:00:00
abstract::An established senescence-accelerated model mouse strain, SAMP8, shows the deterioration of learning and memory compared with a normal control strain, SAMR1. D-Serine binds to strychnine-insensitive glycine binding sites of the N-methyl-D-aspartate (NMDA) receptor complex, and enhances glutamate binding to the recepto...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(98)00059-1
更新日期:1998-08-14 00:00:00
abstract::Lipofuscin-specific autofluorescence of living, cultured rat neonatal myocardial myocytes was studied with respect to intensity and spectral characteristics. The autofluorescence emission spectrum changed over time indicating continuously ongoing intramolecular reorganisation of the pigment. Moreover, as compared with...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)90040-x
更新日期:1994-01-01 00:00:00
abstract::Following a 3 hour in vivo labelling of cytoplasmic RNA in rat liver with orotic acid-6-14C under conditions where ribosomal RNA synthesis was suppressed, the proportion of labelled messenger-like RNA released to the cytoplasm which contained polyadenylate (poly(A)) tracts was about 3.0 times higher in the livers of j...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(77)90026-4
更新日期:1977-07-01 00:00:00
abstract::In this investigation the effect of 4 months of 40% restriction of calories on defined markers of oxidative, glycoxidative or lipoxidative damage to heart mitochondrial proteins was studied. The protein markers assessed were N(epsilon)-(carboxyethyl)lysine (CEL), N(epsilon)-(carboxymethyl)lysine (CML), N(epsilon)-(mal...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(02)00076-3
更新日期:2002-09-01 00:00:00
abstract::The activity of RU 41740, a glycoprotein extract from Klebsiella pneumoniae has been investigated on some polymorphonuclear (PMN) functions. Chemotaxis, random migration and oxidative metabolism (assessed by chemiluminescence, O2 consumption and O2- generation) were studied in parallel. PMN were collected from adult a...
journal_title:Mechanisms of ageing and development
pub_type: 临床试验,杂志文章
doi:10.1016/0047-6374(88)90023-1
更新日期:1988-09-01 00:00:00