Abstract:
:The process of epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) after cataract surgery contributes to tissue fibrosis, wound healing and lens regeneration via a mechanism not yet fully understood. Here, we show that tropomyosin 2 (Tpm2) plays a critical role in wound healing and lens aging. Posterior capsular opacification (PCO) after lens extraction surgery was accompanied by elevated expression of Tpm2. Tpm2 heterozygous knockout mice, generated via the clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) system showed promoted progression of cataract with age. Further, injury-induced EMT of the mouse lens epithelium, as evaluated histologically and by the expression patterns of Tpm1 and Tpm2, was attenuated in the absence of Tpm2. In conclusion, Tpm2 may be important in maintaining lens physiology and morphology. However, Tpm2 is involved in the progression of EMT during the wound healing process of mouse LECs, suggesting that inhibition of Tpm2 may suppress PCO.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Shibata T,Shibata S,Ishigaki Y,Kiyokawa E,Ikawa M,Singh DP,Sasaki H,Kubo Edoi
10.1016/j.mad.2018.03.001subject
Has Abstractpub_date
2018-04-01 00:00:00pages
24-30eissn
0047-6374issn
1872-6216pii
S0047-6374(17)30287-7journal_volume
171pub_type
杂志文章abstract::The healing of normal incisional wounds and ischemic flap wounds was investigated in young (10 weeks) and old (102-104 weeks) rats, together with the effect of treatment with hyperbaric oxygen on day 0-3 of healing. After 10 days of healing all biomechanical strength parameters of normal wounds were decreased by 30-40...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(96)01782-4
更新日期:1996-10-25 00:00:00
abstract::Ageing is characterized by a decline in all systemic functions. A greater susceptibility to apoptosis and senescence may contribute to proliferative and functional impairment of endothelial progenitor cells. They play an important role in neo-angiogenesis and endothelial repair. Vascular ageing is associated with chan...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2016.02.007
更新日期:2016-10-01 00:00:00
abstract::The present study was designed to assess whether treatment with the monoamine oxidase-B (MAO-B) inhibitor L-deprenyl, which has been documented to increase both mean and maximum survival in aged rats as well as sexual performance and cognitive function, has any effect on the age-related microanatomical changes occurri...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)90060-4
更新日期:1994-02-01 00:00:00
abstract::In this article we review the abnormalities in immune function which have been described in relation to ageing. It is suggested that thymic involution may not be the only underlying cause. Many similar changes can be induced by the endotoxins from the cell walls of Gram-negative bacteria. The colon is a large reposito...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/0047-6374(84)90091-5
更新日期:1984-08-01 00:00:00
abstract::We studied the pattern of lectins binding by liver lysosomal proteins from rats between 18 days of gestation and 72 weeks of age. An analysis of the carbohydrate structure was carried out after an electrophoresis and blotting, followed by a very sensitive detection system with highly specific digoxigenin-labelled lect...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(98)00006-2
更新日期:1998-05-01 00:00:00
abstract::This study aimed to assess platelets as a possible model for screening the accumulation of mitochondrial DNA mutations, particularly during normal ageing. For this purpose we isolated platelets from young and old donors selected by lack of systemic and haematological diseases. We studied the accumulation of a particul...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(97)00181-4
更新日期:1998-04-01 00:00:00
abstract::The oral health of older people is changing with reducing numbers of people relying on complete dentures for function, and retaining some natural teeth. Despite this there are substantial numbers of older people whose ability to chew foods is compromised by their oral health status, either because they have few or no ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2004.07.011
更新日期:2004-12-01 00:00:00
abstract::A comparison was made of the resting membrane potential (MP) as well as the MP responses to K+, ACTH and gamma-MSH of superfused adrenocortical, zona fasciculata cells from young (Y) and old (O) male rats. The resting MP of these cells did not vary with age. However, the MP responses to altered extracellular [K+] vari...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(88)90036-x
更新日期:1988-06-01 00:00:00
abstract::Cellular senescence is a phenomenon of irreversible growth arrest in mammalian somatic cells in culture. Various stresses induce cellular senescence and indeed, we have found that excess thymidine effectively induces cellular senescence in human cells. Further, many reports indicate the implication of chromatin protei...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2019.01.001
更新日期:2019-03-01 00:00:00
abstract::Neurons that degenerate in the brains of persons with Alzheimer's disease accumulate mitochondrial amyloid precursor protein (APP), which is thought to negatively affect mitochondrial function and cellular homeostasis. Because proteins that enter mitochondria require assistance from chaperone proteins, we hypothesized...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2009.07.002
更新日期:2009-09-01 00:00:00
abstract::Skeletal muscle ageing is characterized by faulty degenerative/regenerative processes that promote the decline of its mass, strength, and endurance. In this study, we used a transcriptional profiling method to better understand the molecular pathways and factors that contribute to these processes. To more appropriatel...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2009.11.001
更新日期:2010-01-01 00:00:00
abstract::Mycobacteria are an important group of pathogenic bacteria. We generated a series of DNA repair deficient strains of Mycobacterium smegmatis, a model organism, to understand the importance of various DNA repair proteins (UvrB, Ung, UdgB, MutY and Fpg) in survival of the pathogenic strains. Here, we compared tolerance ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2013.08.006
更新日期:2013-10-01 00:00:00
abstract::Age-related changes in blood-brain barrier permeability to macromolecules were investigated during electrically induced seizures. Evans-blue was used as the barrier tracer. There was no change in the permeability of the blood brain barrier associated with aging in the rats. However, the extravasation of Evans-blue alb...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(90)90097-y
更新日期:1990-02-01 00:00:00
abstract::Putrescine, spermidine, spermine and two unknowns designated as A and B were detected in first seedling leaves of barley (Hordeum vulgare L. var. Wolfe). The levels of these polyamines in first seedling leaves from 4-day-old barley plants grown in darkness or in light were comparable and did not change significantly a...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(87)90031-5
更新日期:1987-09-14 00:00:00
abstract::Age-related and ambient temperature-related changes in motor activity, body temperature, body weight (b.w.), and food consumption were studied in the long-lived Peromyscus leucopus mouse at environmental temperatures of 29 and 21 degrees C. Major changes in physiological performance were observed between the young (6 ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(96)01834-9
更新日期:1997-04-01 00:00:00
abstract::A few reports suggest that pigmented granules found in molluscan neurons accumulate with age as do lipofuscin granules in vertebrate cells; however, no reports on molluscan neurons include detailed descriptions of granule accumulation or histochemical tests to identify the pigment as lipofuscin-like. In this study lig...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(78)90052-0
更新日期:1978-01-01 00:00:00
abstract::Ageing is the most complex phenotype currently known, since it becomes manifest in all organs and tissues, affects an organism's entire physiology, impacts function at all levels and increases susceptibility to all major chronic diseases. Insight into the molecular and cellular targets of the ageing process would offe...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/s0047-6374(02)00163-x
更新日期:2003-01-01 00:00:00
abstract::Exposure of Drosophila melanogaster adult males to an atmosphere of O2-N2 (1:1) at 760 Torr resulted in a marked reduction of life span. Among the fine structural changes occurring in the oxygen-poisoned flies, the following were similar to age-related changes: ceroid-lipofuscin accumulation in the visceral organs, sp...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(75)90006-8
更新日期:1975-01-01 00:00:00
abstract::Endothelin (ET) is a powerful vasopressor agent that is activated in a number of pathophysiologic states where renal perfusion is reduced. Since renal vasoconstriction occurs as part of renal aging, we investigated the possibility that ET may be activated in the old kidney. These experiments involved acutely blocking ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(97)00171-1
更新日期:1998-03-16 00:00:00
abstract::The responses of the left and right ventricles (LV and RV) to physical conditioning in cold (25 degrees C) and thermoneutral temperatures (35 degrees C), with special reference to lactate dehydrogenase (LDH) and its isoenzyme profile, were studied in the 2-month (young)- and 12-month (middle-aged)-old rats. Moderate h...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(99)00004-4
更新日期:1999-04-01 00:00:00
abstract::Older adults who are at risk of developing influenza illness, have a low level of influenza virus-stimulated cytotoxic T lymphocyte (CTL) activity as measured by an assay of granzyme B (GrB). The purpose of this study was to determine whether aging affected memory CTL populations identified by GrB expression in influe...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2007.05.001
更新日期:2007-05-01 00:00:00
abstract::We have studied effects of long-term, low-dose growth hormone therapy on the immune function and life expectancy of Balb/c mice. Sixty male Balb/c mice were aged up to the time when they started showing signs of senescence and causal death (deaths started when they became 17 months old). The aged mice were divided int...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(91)90026-v
更新日期:1991-01-01 00:00:00
abstract::In the rat, kininogens are produced from two distinctly regulated gene families, T- and K-kininogens. We have previously established that T-kininogen (T-KG, thiostatin) gene expression is increased in the liver of old rats, as compared to their younger counterparts. In this report, we have used Northern blot analysis ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(95)01639-2
更新日期:1995-10-13 00:00:00
abstract::The present study was designed to determine whether the memory sparing effects of dietary restriction during aging could be through an effect on ionotropic glutamate receptors. Quantitative autoradiography was performed on 3, 10, and 26 month old mice to examine the density changes of NMDA, AMPA and kainate binding si...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(97)01884-8
更新日期:1997-05-01 00:00:00
abstract::At various time intervals (10, 15, 20, 25, 30 min) after injection of 3H-fucose into the medial septal nucleus of young adult (3 months old) and senescent (25 months old) Fischer-344 rats, the specific activities of trichloroacetic acid-phosphotungstic acid (TCA-PTA) soluble and insoluble fractions were determined in ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(77)90038-0
更新日期:1977-09-01 00:00:00
abstract::Motor neurons and ventral root motor fibres innervating extensor digitorum longus (EDL) muscle in rats of 0.5-3.5 months of age were studied by HRP-retrograde labelling and in vivo muscle tension recording, respectively. EDL nucleus size increased with age but motor neurons number and size did not change. Twitch and t...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(96)01839-8
更新日期:1996-12-20 00:00:00
abstract::This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of Marie-Ange Teste, Isabelle Léger-Silvestre, Jean M François and Jean-Luc Parrou. Marjorie Petitjean could not b...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,收录出版
doi:10.1016/j.mad.2016.07.011
更新日期:2017-01-01 00:00:00
abstract::The strychnine-insensitive binding of [3H]glycine was characterized in purified cerebral cortical membranes from mice aged from 7 days to 22 months. The binding was saturable, exhibiting only one component during the whole life-span studied. The binding constant KD did not change during development and ageing, whereas...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(93)90131-a
更新日期:1993-11-01 00:00:00
abstract::At least five different transgenic approaches have been applied to the study of Drosophila aging. There are two single component systems: transgenes with native (normal) promoters and transgenes with heterologous promoters; as well as three binary systems: 'GAL4/UAS', 'FLP-out' and 'tet-on'. These approaches vary in a...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/s0047-6374(00)00152-4
更新日期:2000-09-01 00:00:00
abstract::Researchers and clinicians interested in human diseases of DNA repair deficiency and premature aging gathered at the National Conference Center in Lansdowne, Virginia on 5-8 September 2006 to attend a workshop co-organized by Vilhelm Bohr (National Institute of Aging) and Kenneth Kraemer (National Cancer Institute). A...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2006.11.028
更新日期:2007-02-01 00:00:00