Abstract:
:Around the onset of labor, uterine sensitivity to oxytocin (OT) increases tremendously. Although this is considered to reflect OT receptor (OTR) augmentation in myometrium, neither spatial expression of OTR nor the level of the receptor message during the course of pregnancy have been investigated at the molecular level. We examined the localization and expression of the OTR in human myometrium by means of in situ hybridization, immunohistochemistry, and Northern and Western blotting. In the term pregnant myometrium, OTR expressing smooth muscle cells are observed diffusely and heterogeneously. Some of the smooth muscle cells were expressed high levels of the receptor at the messenger RNA and protein level, and they were surrounded with cells weakly positive for the OTR or negative. The level of OTR transcripts increased according to the course of pregnancy. The receptor messenger RNA level reached over 300-fold at parturition compared with the nonpregnant myometrium. In the myometrium at 32 weeks of gestation and not in labor, a relatively large amount (about 100-fold) of the receptor message was expressed. In the nonpregnant myometrium, significant amount of the receptor protein was revealed by Western blotting. We also found that the receptor protein was augmented at term and after the onset of labor. These findings indicated that the expression of OTR changes dynamically at the transcription and protein level during pregnancy and that its expression is heterogeneous in the term myometrium.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Kimura T,Takemura M,Nomura S,Nobunaga T,Kubota Y,Inoue T,Hashimoto K,Kumazawa I,Ito Y,Ohashi K,Koyama M,Azuma C,Kitamura Y,Saji Fdoi
10.1210/endo.137.2.8593830subject
Has Abstractpub_date
1996-02-01 00:00:00pages
780-5issue
2eissn
0013-7227issn
1945-7170journal_volume
137pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::For insight into transcriptional mechanisms mediating physiological responses to GH, data mining was performed on a profile of GH-regulated genes induced or inhibited at different times in highly responsive 3T3-F442A adipocytes. Gene set enrichment analysis indicated that GH-regulated genes are enriched in pathways in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0212
更新日期:2009-08-01 00:00:00
abstract::Hypothalamic proopiomelanocortin (POMC)-derived MSH peptides and the melanocortin receptor antagonist, agouti-related protein (AgRP), interact to regulate energy balance. Both POMC and AgRP neurons express estrogen receptors, but little is known about estrogen regulation of the melanocortin system in the primate. We h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0853
更新日期:2010-03-01 00:00:00
abstract::GH is considered to play a role in the pathogenesis of diabetic retinopathy, causing neovascularization in the retina. The present study was conducted to assess the possibility that GH may play a role in ocular development by determining whether GH is expressed in the eye of the chicken during development. In the 17-d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8552
更新日期:2001-12-01 00:00:00
abstract::Dexamethasone-induced changes in insulin and epidermal growth factor (EGF) receptor number, autophosphorylation, and kinase activity were studied in intact rat hepatocytes. Hepatocytes were freshly isolated from Sprague-Dawley rats treated with dexamethasone (1 mg/kg) for 4 days and from untreated littermates. Dexamet...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-5-2214
更新日期:1988-11-01 00:00:00
abstract::An assay was developed to detect tritium-labeled ovine FSH beta-subunit [( 3H]oFSH beta) secreted from primary ovine pituitary cultures. This procedure used affinity-enriched antibodies raised against reduced and carbamylmethylated oFSH beta (RCM-oFSH beta) in a two-cycle immunoextraction procedure. A discrete species...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-154
更新日期:1984-07-01 00:00:00
abstract::Homeostatic control of endocrine systems proceeds via feedforward (agonistic, stimulatory) and feedback (antagonistic, inhibitory) interactions mediated via implicit dose-response functions. However, neither the feedback/feedforward pathways nor the dose-response interfaces are directly observed in vivo. Thus, the goa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1461
更新日期:2011-12-01 00:00:00
abstract::Glucagon-like peptide 1 (7-36)amide (GLP-1) is an insulinotropic intestinal peptide hormone with a potential role as antidiabetogenic therapeutic agent. It mediates a potentiation of glucose-induced insulin secretion, by activation of adenylate cyclase and subsequent elevation of cytosolic free calcium, [Ca2+]cyt. We ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6947
更新日期:1999-09-01 00:00:00
abstract::1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] inhibits proliferation of normal and malignant prostate epithelial cells at least in part through inhibition of G1 to S phase cell cycle progression. The mechanisms of the antiproliferative effects of 1,25-(OH)2D3 have yet to be fully elucidated but are known to require the vita...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0434
更新日期:2010-10-01 00:00:00
abstract::Food deprivation in laboratory rats induces profound changes in the neuroendocrine system. We have investigated the hypothalamic and pituitary responses of the hypothalamo-pituitary thyroid axis to 48-h food deprivation in Sprague-Dawley rats. Peripheral T3 and hypophysial portal TRH were measured by RIA, and TSH beta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-5-2714
更新日期:1991-11-01 00:00:00
abstract::The ghrelin receptor (GhrR) is known for its strong orexigenic effects in pharmacological doses and has long been considered as a promising target for the treatment of obesity. Several antagonists have been developed to decrease the orexigenic signaling, but none of these have been approved for the treatment of obesit...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqaa020
更新日期:2020-04-01 00:00:00
abstract::Hypothyroidism is the most frequent and earliest endocrine complication in cystinosis, a multisystemic lysosomal storage disease caused by defective transmembrane cystine transporter, cystinosin (CTNS gene). We recently demonstrated in Ctns(-/-) mice that altered thyroglobulin biosynthesis associated with endoplasmic ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1762
更新日期:2016-04-01 00:00:00
abstract::We have previously shown that dopamine-evoked inhibition of corticosteroid production from adrenocortical cells is mediated through a decrease in prostaglandin biosynthesis. Since the catecholamine did not alter the stimulatory effect of arachidonic acid, it was proposed that dopamine may inhibit the formation of arac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2625
更新日期:1991-05-01 00:00:00
abstract::Addition of somatostatin-14 (SRIF) inhibits corticotropin releasing factor (CRF) and forskolin-stimulated cyclic AMP formation and ACTH release from tumor cells of the mouse anterior pituitary (AtT-20/D16-16). After long-term pretreatment of these cells with SRIF, the ability of SRIF to inhibit CRF and forskolin-stimu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-2-811
更新日期:1983-08-01 00:00:00
abstract::Progesterone has the capacity to suppress hypothalamic dopaminergic neuronal activity on proestrous afternoon and prolong or amplify the preovulatory prolactin surge in rats. In the present study, we examined enzyme activity and phosphorylation state of tyrosine hydroxylase (TH) in the stalk-median eminence of cycling...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1345
更新日期:2008-04-01 00:00:00
abstract::Reduced and S-carbamidomethylated human GH (RCAM-hGH) was digested with human plasmin, yielding a mixture of products. These were partially separated by chromatography on DEAE-cellulose, yielding three major fractions: Da and Db, which were equipotent with native hGH in the weight gain test; and Dc, which was about ha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-5-1366
更新日期:1978-05-01 00:00:00
abstract::Brain natriuretic peptide (BNP) and atrial natriuretic peptide (ANP) are present in brain regions regulating LH secretion. Similarities in the molecular structure of these peptides suggest similar physiological function within the brain. This study examined the effects of centrally administered BNP and ANP on LH secre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-801
更新日期:1991-08-01 00:00:00
abstract::Under acute stress conditions in the rat, there is rapid and transient increase in circulating prolactin (PRL). This leads to an elevated expression of the long form of PRLR (PRLR-L) first in the hypothalamus and the choroid plexus. This increase in PRL is involved in the inhibition of stress-induced hypocalcemia and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1528
更新日期:2005-08-01 00:00:00
abstract::The GH/IGF-I axis has both pre- and postpubertal metabolic effects. However, the differential effects of GH and/or IGF-I on animal physiology or the plasma proteome are still being unraveled. In this report, we analyzed several physiological effects along with the plasma proteome after treatment of mice with recombina...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1217
更新日期:2011-10-01 00:00:00
abstract::Previous studies indicate that magnesium, like calcium, stimulates the release of calcitonin (CT) from the thyroid gland. On the other hand, C-cell hyperplasia has been noted in magnesium-deficient dogs and rats. To explore further possible interrelationships between magnesium and CT, 21-day-old Sprague-Dawley male ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2232
更新日期:1985-06-01 00:00:00
abstract::Newborn male rats were treated SC with dexamethasone phosphate (100 microgram) or dexamethasone acetate (100 microgram) on postnatal day 1, dexamethasone phosphate (5.0 microgram) opn postnatal days 1, 2, 3, or the corresponding drug vehicle. All animals were sacrificed 65-70 days later at previously determined daily ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-1055
更新日期:1981-03-01 00:00:00
abstract::Deciduomal reactions in response to artificial stimuli were induced on the tube-ligated uterine horns of unilateral pregnant hamsters. Serum progesterone levels were determined, and the changes of estrogen and progesterone receptors (ER and PR, respectively) were simultaneously studied in the deciduoma. After stimulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-302
更新日期:1989-07-01 00:00:00
abstract::The endocrine-disrupting chemical bisphenol A (BPA) increases adipose tissue mass in vivo and promotes adipogenesis in vitro; however, mechanisms explaining BPA's obesogenic effect remain unknown. We investigated the effects of gestational BPA and its analog, bisphenol S (BPS), exposure on the adipogenic differentiati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00615
更新日期:2017-11-01 00:00:00
abstract::The ability of cells to directly respond to glucocorticoids and aldosterone is a function of GR and MR expression, and coexpression of 11beta-hydroxysteroid dehydrogenases (11betaHSDs), which convert glucocorticoids and their 11-ketometabolites into either receptor inactive or active derivatives. The aim of the presen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.1.8583
更新日期:2002-01-01 00:00:00
abstract::To study the physiological regulation of the iodothyronine 5'-deiodinases (I-5'D), we have examined the effects of some thiol blockers and of thyroid status on I-5'D activities both in vitro and in vivo. At low (less than 5 mM) concentrations of dithiothreitol, propylthiouracil (PTU) inhibited I-5'D in the brain, pitu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2597
更新日期:1990-05-01 00:00:00
abstract::Intraluteal infusion of the prostaglandin (PG) synthesis inhibitor, sodium meclofenamate (Mec) causes premature luteolysis in rhesus monkeys. To evaluate further the actions of PG synthesis inhibitors in primate luteal function, we examined the in vitro effects of Mec and another inhibitor, flurbiprofen (Flur), on PG,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1380
更新日期:1990-03-01 00:00:00
abstract::This study examined the requirement for 17 beta-estradiol (E) and relaxin (R) during the preparturient period (approximately the last 30 h of gestation) for normal parturition and pup survival in the rat. On the evening of day 21 of pregnancy, primiparous Sprague-Dawley rats were bilaterally ovariectomized or sham ope...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1958
更新日期:1988-05-01 00:00:00
abstract::We studied the effect of peroxisome proliferator-activated receptor-alpha (PPAR-alpha) activation on serum concentrations and tissue expression of resistin, adiponectin, and adiponectin receptor-1 and -2 (AdipoR1 and AdipoR2) mRNA in normal mice and mice with insulin resistance induced by lipogenic, simple-carbohydrat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1624
更新日期:2006-09-01 00:00:00
abstract::We have previously demonstrated that the gonadotropin-mediated inhibition of apoptosis in ovarian granulosa cells is linked to changes in the expression of several cell death-related genes, including members of the bcl-2 gene family (bcl-2, bax, and bcl-x). Recently, the product of the p53 tumor suppressor gene, a pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.4.7895650
更新日期:1995-04-01 00:00:00
abstract::We investigated the effects of the peripheral administration of 17 beta-estradiol (E2), estrone (E1), and the catecholestrogens, 2-hydroxyestradiol (2-OHE2) and 2-hydroxyestrone, (2-OHE1), on anterior pituitary gland PRL release in the prepuberal rat. Steroids in oil were injected sc into 25-day-old female and 35- to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-2-325
更新日期:1982-02-01 00:00:00
abstract::After 5 days of incubation with 10 nM tritiated dexamethasone, the amount of glucocorticoid displaceably-bound by the ACTH-secreting, glucocorticoid-responsive AtT-20 mouse pituitary tumor cell decreases by 70-80%. This decrease takes place in both the cytosolic and nuclear fractions. Although one might predict that t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-2-699
更新日期:1982-08-01 00:00:00