Abstract：
:A useful approach for constructing dose-response relationships and for studying the underlying mechanisms by which a xenobiotic agent enhances airway reactivity is to measure the response of an isolated airway following ex vivo exposure to a pollutant. We have in this way determined the dose-response relationship between ex vivo exposure to pollutants such as nitrogen dioxide (NO2), the aldehyde acrolein, and ozone (O3) and the reactivity to agonists in human isolated bronchial smooth muscle. We have also investigated the underlying alteration in the cellular mechanisms of airway smooth-muscle contraction induced by such exposure and found that it is related to alteration in calcium signaling at the site of the airway smooth-muscle cell. Finally, although there is epidemiological evidence that an increase in allergic diseases such as asthma may be linked to air pollution, there are few experimental data to address this issue. The final aim of this study was therefore to investigate the interaction between passive sensitization and exposure to pollutants in human isolated airways. We have examined (i) the effect of a pre-exposure to pollutants on the contraction of sensitized bronchi in response to a specific antigen and (ii) the effect of passive sensitization on the contraction in response to nonspecific agonists in bronchi pre-exposed to pollutants. The results indicate a combined effect of immunological sensitization and exposure to pollutants; that is, passive sensitization and exposure to pollutants act in a synergistic manner on human bronchial smooth-muscle reactivity in response to both specific antigens and nonspecific agonists.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Roux E,Ouedraogo N,Hyvelin JM,Savineau JP,Marthan Rdoi
10.1023/a:1019514830173subject
Has Abstractpub_date
2002-01-01 00:00:00pages
289-99issue
5eissn
0742-2091issn
1573-6822journal_volume
18pub_type
杂志文章abstract：:Recent findings suggest a functional interaction of the drug resistance enzyme glutathione S-transferase P (GSTP) with the transcription factor Nrf2, a master regulator of the adaptive stress response to cellular electrophiles. The effect of this interaction on the metabolism and redox of cellular thiols was investiga...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09517-5
更新日期：2020-08-01 00:00:00
abstract：:Folic acid (FA) is a vitamin essential for normal cellular functions, growth, and development. Because humans cannot synthesize this micronutrient, it must be obtained from dietary sources through intestinal absorption. The intestinal tract is a major target for oxidative stress. Our aim was to investigate the effect ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-012-9228-8
更新日期：2012-12-01 00:00:00
abstract：:Cellular accumulation of mono(2-ethylhexyl)phthalate (MEHP) has been recently demonstrated to disturb fat cell energy metabolism; however, the underlying mechanism remained unclear. The study aimed to determine how MEHP influenced fat cell transcriptome and how the changes might contribute to bioenergetics. Because of...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-016-9380-7
更新日期：2017-12-01 00:00:00
abstract：:Cd2+ provokes inositol trisphosphate production and releases stored Ca2+, apparently by binding to a zinc site in the external domain of an orphan receptor. One microM Cd2+ evokes an immediate spike in cytosolic free Ca2+, which is similar to that evoked by bradykinin. Platelet-derived growth factor (PDGF) also increa...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00757576
更新日期：1993-04-01 00:00:00
abstract：:Glioblastoma (GBM) is the most aggressive type of glioma. Temozolomide (TMZ) is currently the drug of choice used for post-operative chemotherapy of GBM. However, the presence of intrinsic and acquired resistance hinders the success of chemotherapy. To understand the TMZ resistant mechanisms in glioma, we investigated...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-019-09502-7
更新日期：2020-06-01 00:00:00
abstract：:During the last decade, the unbound glutathione content of cultured adherent cells has become a very important biological marker for many pharmacological and toxicological in vitro studies with regard to the protective role of the tripeptide in its reduced form (GSH). However, the literature does not provide extensive...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00767495
更新日期：1995-04-01 00:00:00
abstract：:A tissue system in which fibroblasts and myocytes from chick embryonic hearts were separately maintained was used to study the toxicity of ethanol. To reproduce the teratogenic effects of acute, high concentrations of ethanol typical of "binge" drinking, an open tissue culture system was employed. With open cultures, ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00119291
更新日期：1992-01-01 00:00:00
abstract：:Aging, injuries, and diseases can be considered as the result of malfunctioning or damaged cells. Regenerative medicine aims to restore tissue homeostasis by repairing or replacing cells, tissues, or damaged organs, by linking and combining different disciplines including engineering, technology, biology, and medicine...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-017-9384-y
更新日期：2017-08-01 00:00:00
abstract：:The mechanism by which lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA) induces production of proinflammatory cytokines in murine macrophages, and the role of phosphatidylinositol 3-kinase (PI3-kinase) have not been well investigated. Activation of nuclear factor kappaB (NF-kappaB) is initiated by the...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1015384201785
更新日期：2002-01-01 00:00:00
abstract：:Because of a possible role of astrocytes in trialkyltin-induced neurotoxicity in vivo various studies have been performed using cultures of astrocytes or glioma cells in vitro. With respect to cytotoxic potencies of trialkyltins these studies gave rather divergent results. Therefore the aim of the present study was to...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-004-3859-3
更新日期：2004-09-01 00:00:00
abstract：:Clinical tests of gene sequence, structure, and function are to predict, diagnose, monitor, and prognose human disease-specific phenomes, characters severities, durations, stages, and responses to therapy. The concept and content of gene tests for clinical application mainly include chromosome/chromatins, DNA, and RNA...
journal_title：Cell biology and toxicology
pub_type： 社论
doi：10.1007/s10565-019-09464-w
更新日期：2019-04-01 00:00:00
abstract：:In pharmaceutical research, in vitro toxicity tests, for assessing the potential toxicity of new chemical entities are necessary in the early stages of the developmental process, when no information is available about the metabolism or even the target organ toxicity of the compounds to be tested. In vitro specific org...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1023/a:1010958121396
更新日期：2001-01-01 00:00:00
abstract：:Cholestatic liver injury, a group of diseases characterized with dysregulated bile acid (BA) homeostasis, was partly resulted from BA circulation disorders, which is commonly associated with the damage of hepatocyte barrier function. However, the underlying hepatocyte barrier-protective molecular mechanisms of cholest...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09567-9
更新日期：2021-01-05 00:00:00
abstract：:Ziram, a zinc dithiocarbamate is widely used worldwide as a fungicide in agriculture. In order to investigate ziram-induced changes in macrophage functions and polarization, human monocytes-derived macrophages in culture were treated with ziram at 0.01-10 μmol.L-1 for 4-24 h. To characterize zinc involvement in these ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09540-6
更新日期：2020-07-25 00:00:00
abstract：:Initiated/selected (ISH) and normal (NH) rat hepatocytes were used to study cytoskeleton modifications induced by three liver acting chemicals: 2-AAF, a liver complete carcinogen; PB, a liver tumor promoter; and 4-AAF, a non-carcinogen analogue of 2-AAF. Cytoskeleton alterations were visualized by disappearance of F-a...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00755140
更新日期：1993-01-01 00:00:00
abstract：:Based on the reduced expression of ethanol-oxidizing enzymes in human hepatocellular carcinoma (HepG2) cells, we analyzed the role of nonoxidative metabolites in ethanol-induced apoptosis in HepG2 cells. For this purpose, an analysis of volatile metabolites of ethanol using ion-mobility spectrometry and gas chromatogr...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9009-y
更新日期：2007-11-01 00:00:00
abstract：:Human tumor cells have properties in vitro or in surrogate hosts that are distinct from those of normal cells, such as immortality, anchorage independence, and tumor formation in nude mice. However, different cells from individual tumors may exhibit some, but not all of these features. In previous years, human tumor c...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007419810705
更新日期：1997-10-01 00:00:00
abstract：:We compared the effects of four quaternary benzo[c]phenanthridine alkaloids--chelerythrine, chelilutine, sanguinarine, and sanguilutine--and two quaternary protoberberine alkaloids-berberine and coptisine--on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomy...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1010907231602
更新日期：2001-01-01 00:00:00
abstract：:In a number of adverse drug reactions leading to hepatotoxicity drug metabolism is thought to be involved by generation of reactive metabolites from nontoxic drugs. In this study, an in vitro assay was developed for measurement of the impact of metabolic activation of compound on the cytotoxicity toward a human hepati...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9018-x
更新日期：2008-01-01 00:00:00
abstract：:Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms (HAB), belongs to the Diarrheic Shellfish Poisoning toxins that cause gastrointestinal symptoms in humans after consumption. In the present work, Ruditapes decussatus haemocytes were selected to evaluate the effect of OA on cell viability, enzy...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9246-1
更新日期：2013-06-01 00:00:00
abstract：:A method for the primary culture of rat liver cells on collagen-coated dextran microcarriers is described. Ethoxycoumarin deethylase (EOD) activity 24 hr after inoculation was comparable for liver cells cultured on microcarriers and on collagen-coated dishes. Cells were cultured on microcarriers for up to 48 hr and re...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00124073
更新日期：1991-10-01 00:00:00
abstract：:An in vitro human nasal model was developed as a tool to study the local tolerability of nasal powder forms using excised nasal mucosa in a diffusion chamber. The suitability of this model was tested using Sandostatin (SMS) an octapeptide analog of somatostatin, as a reference drug enhanced by Avicel (microcrystalline...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00757627
更新日期：1995-10-01 00:00:00
abstract：:We investigated mitogen-activated protein kinase (MAPK) pathways as well as reactive oxygen species (ROS) in olaquindox-induced apoptosis. Exposure of HepG2 cells to olaquindox resulted in the phosphorylation of p38 MAPK and c-Jun N-terminal kinases (JNK). To confirm the role of p38 MAPK and JNK, HepG2 cells were pret...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9249-y
更新日期：2013-08-01 00:00:00
abstract：:Ten compounds representative of diverse classes of chemicals were evaluated for their cytotoxicity and transforming ability to human skin fibroblasts in vitro. Only five of the ten compounds were highly cytotoxic in the 0-100 micrograms/ml range and their order of cytotoxicity was: 2,5-bis(1-aziridinyl)-3,6-bis(carboe...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00249592
更新日期：1990-04-01 00:00:00
abstract：:The piperidine nitroxides Tempamine and Tempace have been studied for their effect on doxorubicin (DOX) and hydrogen peroxide (H(2)O(2)) cytotoxicity in immortalized B14 cells, a model for neoplastic phenotype. The significance for nitroxide performance of the substituent in position 4 of the piperidine ring was evalu...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9020-3
更新日期：2008-01-01 00:00:00
abstract：:During the last few years the increased presence of proinflammatory cytokines and oxidation products in Alzheimer's disease (AD) has been largely described. Oxysterols, formed by hydroxylation of cholesterol, occur naturally in the brain and are increased in the serum of AD patients. Of these oxysterols, 25-hydroxycho...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1011996515328
更新日期：2001-01-01 00:00:00
abstract：:Recepteur d'origine nantais (RON) has been implicated in cell proliferation, metastasis, and chemoresistance of various human malignancies. The short-form RON (sf-RON) encoded by RON transcripts was overexpressed in gastric cancer tissues, but its regulatory functions remain illustrated. Here, we found that sf-RON pro...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09525-5
更新日期：2021-02-01 00:00:00
abstract：:Generating large amounts of recombinant protein in transgenic animals is often challenging and has a number of drawbacks compared to cell culture systems. The baculovirus expression vector system (BEVS) uses virus-infected insect cells to produce recombinant proteins to high levels, and these are usually processed in ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-009-9133-y
更新日期：2010-02-01 00:00:00
abstract：:Cyclosporin A induces in vivo a severe nephrotoxicity characterized by a large decrease in renal hemodynamics. The aim of this study is to establish the ability of the known NO donor 3-morpholinosydnonimine (SIN-1) to prevent the cyclosporin A-induced contraction by using rat isolated glomeruli and cultured glomerular...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00438167
更新日期：1996-12-01 00:00:00
abstract：:Drug-induced liver injury (DILI) is a leading cause of discontinuation of new drug approval or withdrawal of marketed medicine based on safety due to organ vulnerability. The aim of this research is to investigate the potential abilities of four different in vitro cell models (L-02, HepG2, HepaRG, and hiHeps cell line...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-016-9316-2
更新日期：2016-02-01 00:00:00
