Abstract：
:Folic acid (FA) is a vitamin essential for normal cellular functions, growth, and development. Because humans cannot synthesize this micronutrient, it must be obtained from dietary sources through intestinal absorption. The intestinal tract is a major target for oxidative stress. Our aim was to investigate the effect of oxidative stress upon the uptake of FA by Caco-2 cells. Oxidative stress was induced by exposure of the cells to tert-butyl hydroperoxide (TBH) for 1 h. TBH (3,000 μM) induced an increase in biomarkers of oxidative stress, while maintaining cell viability and proliferation. In relation to the apical uptake of (3)H-FA, TBH (3,000 μM) reduced the cellular accumulation of (3)H-FA (10 nM), although the characteristics (kinetics, pH dependence, and inhibitory profile) of (3)H-FA uptake were not changed. This effect was associated with a decrease in the mRNA steady-state levels of proton-coupled folate transporter and folate receptor alpha and of the efflux transporter multidrug resistance protein 2. Moreover, TBH (3,000 μM) did not affect the noncarrier-mediated apical uptake of (3)H-FA. Finally, the effect of TBH upon (3)H-FA apical uptake was not dependent on protein kinase A, protein kinase C, mitogen-activated protein kinases, phosphoinositide 3-kinase, nuclear factor kappa B, and protein tyrosine kinases, but was completely prevented by dietary polyphenols (resveratrol, quercetin, and EGCG). These results suggest that oxidative stress at the intestinal level may result in a reduction in the intestinal absorption of dietary FA and that polyphenolic dietary components may offer protection against oxidative stress-induced inhibition of intestinal FA absorption.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Couto MR,Gonçalves P,Catarino T,Araújo JR,Correia-Branco A,Martel Fdoi
10.1007/s10565-012-9228-8subject
Has Abstractpub_date
2012-12-01 00:00:00pages
369-81issue
6eissn
0742-2091issn
1573-6822journal_volume
28pub_type
杂志文章abstract：:In previous studies cadmium chloride (CdCl2) nonlethally inhibited Y-1 adrenal mouse adrenal tumour cell 20-dihydroxyprogesterone (20DHP) secretion, affecting unstimulated and stimulated steroidogenic pathway sites differently. We studied CdCl2 effects on unstimulated steroidogenesis using Y-1 cells incubated 0.5 h in...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00757184
更新日期：1994-02-01 00:00:00
abstract：:Manganese exposure is among the many environmental risk factors linked to the progression of neurodegenerative diseases, such as manganese-induced parkinsonism. In animal models, chronic exposure to manganese causes loss of cell viability, neurodegeneration, and functional deficits. Polyamines, such as spermine, have ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-018-09449-1
更新日期：2019-04-01 00:00:00
abstract：:Microcystin-leucine-arginine (MC-LR) was produced by toxic cyanobacteria, which has been shown to have potent hepatotoxicity. Our previous study has proved that MC-LR were able to promote intrahepatic biliary epithelial cell excessive proliferation. However, the underlying mechanism is not yet entirely clarified. Here...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09575-9
更新日期：2021-01-21 00:00:00
abstract：:Pulmonary alveolar macrophages exposed to very short chrysotile asbestos fibers present a typical cytotoxic response: extracellular releases of lactate dehydrogenase and beta-galactosidase, and a decrease in cellular ATP content. The objective of this study was to determine if nicotinamide and 3-aminobenzamide, two in...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00141284
更新日期：1988-03-01 00:00:00
abstract：:The response of fatty liver to stress conditions (t-butyl hydroperoxide [t-BH] or 36 h of fasting) was investigated by assessing intracellular glutathione (GSH) compartmentation and redox status, GSH peroxidase (GSH-Px) and reductase (GSSG-Rx) activities, lipid peroxidation (TBARs) and serum ALT levels in rats on a ch...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/b:cbto.0000013341.73139.fc
更新日期：2003-11-01 00:00:00
abstract：:Bisnaphthalimido compounds bis-intercalate to DNA via the major groove and are potentially potent cancer therapeutics. Previously, we incorporated natural polyamines as linkers connecting the two naphthalimido ring moieties to create a series of soluble bisnaphthalimidopropyl polyamines (BNIPPs). Here, extending earli...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9199-1
更新日期：2011-12-01 00:00:00
abstract：:Gap junctions (GJ) represent a cellular communication system known to influence neuronal differentiation and survival. To assess a putative role of this system for neural effects of tamoxifen (TAM) and raloxifene (RAL), we used the human teratocarcinoma cell line NTera2/D1, retinoic acid (RA)-dependent neuronal differ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-010-9165-3
更新日期：2010-12-01 00:00:00
abstract：:S-(chloroethyl)-cysteine (CEC) and S-(1,2-dichlorovinyl)-cysteine (DCVC) have been proposed as intermediates in the metabolic transformation of the carcinogens 1,2-dichloroethane and 1,1,2-trichloroethylene. We have tested the ability of CEC and DCVC to induce DNA repair and genotoxic effects at the chromosomal level ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117768
更新日期：1988-12-01 00:00:00
abstract：:Cd2+ provokes inositol trisphosphate production and releases stored Ca2+, apparently by binding to a zinc site in the external domain of an orphan receptor. One microM Cd2+ evokes an immediate spike in cytosolic free Ca2+, which is similar to that evoked by bradykinin. Platelet-derived growth factor (PDGF) also increa...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00757576
更新日期：1993-04-01 00:00:00
abstract：:We assessed the in vitro toxicity of various particles on three murine macrophage cell lines, (J774A.1, WR19M.1, RAW264.7). The cells were exposed to aqueous suspensions (0-100 microg/30 mm2 well) of urban particulate matter (SRM-1648, SRM-1649, EHC-93), fine particulate matter (PM2.5), titanium dioxide (SRM-154b), an...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/b:cbto.0000038461.02222.95
更新日期：2004-07-01 00:00:00
abstract：:Mechanisms of cell death induced by toxins probably involve one or more processes such as inhibition of protein synthesis and impairment of plasma membrane integrity leading to an increase in membrane permeability. Since one of the possible actions of mastoparan, a cationic tetradecapeptide from wasp venom, is to pert...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00135029
更新日期：1990-01-01 00:00:00
abstract：:Skin intoxication by bis(beta-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007491703271
更新日期：1998-02-01 00:00:00
abstract：:DNA formulated into aggregates with polycationic reagents are referred to by a variety of terms including non-viral vectors, synthetic vectors, lipoplexes, polyplexes and more recently nanoparticles. The capacity for delivery of multiple genes, genomic-sized constructs and siRNA delivery, with a diversity of possible ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-009-9141-y
更新日期：2010-02-01 00:00:00
abstract：:The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117825
更新日期：1987-03-01 00:00:00
abstract：:Presently, a large effort is being made worldwide to increase the sustainability of industrial development, while preserving not only the quality of the environment but also that of animal and human life. In this work, sea urchin early developmental stages were used as a model to test the effects of the organophosphat...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-008-9061-2
更新日期：2008-12-01 00:00:00
abstract：:In order to clarify the protective mechanism of sodium molybdate against the acute toxicity of cadmium chloride in rat, the effect of in vivo sodium molybdate pretreatment on the cytotoxic action of cadmium in isolated hepatocytes was studied. The cytosolic pH of hepatocytes isolated from untreated rats immediately de...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00124071
更新日期：1991-10-01 00:00:00
abstract：:DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) including the maintenance enzyme DNMT 1 and de novo methyltransferases DNMT 3a and DNMT 3b. Elevated levels of DNMTs have been found in cancer cells and in several types of human tumors. A polymorphism found in DNMT3b has been associated with i...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9035-9
更新日期：2008-06-01 00:00:00
abstract：:The mutation rates of tumor suppressor protein p53 gene (TP53) are high in lung adenocarcinoma and promote the development of acquired drug resistance. The present study evaluated the p53-dependent role in lung cancer cell sensitivity to PI3K-specific inhibitors, PI3K-associated inhibitors, PI3K-non-related inhibitors...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09523-7
更新日期：2020-10-01 00:00:00
abstract：:The histopathological response and cell culture characteristics of liver cells from the R16 (grc-) strain of rats, which carries an MHC-linked deletion, were examined one week after a single intraperitoneal injection of 200 mg/kg body weight diethylnitrosamine (DEN) and were compared with the response of liver cells f...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00249594
更新日期：1990-04-01 00:00:00
abstract：:The goal of cancer eradication has been overshadowed despite the continuous improvement in research and generation of novel cancer therapeutic drugs. One of the undeniable existing problems is drug resistance due to which the paradigm of killing all cancer cells is ineffective. Tumor microenvironment plays a crucial r...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-019-09461-z
更新日期：2019-10-01 00:00:00
abstract：:The article Is the clinical lipidomics a potential goldmine?, written by Linlin Zhang, Xianlin Han and Xiangdong Wang, was originally published electronically on the publisher's internet portal (currently SpringerLink) on 21 July 2018 with open access. With the author(s)' decision to step. ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s10565-019-09490-8
更新日期：2020-06-01 00:00:00
abstract：:Autophagy is a conserved lysosomal degradation process, and abnormal autophagy has been associated with various pathological processes, e.g., neurodegeneration, cancer, and pathogen infection. Small chemical modulators of autophagy show the potential to treat autophagy-associated diseases. Diterpenoids, nature product...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-021-09580-6
更新日期：2021-01-23 00:00:00
abstract：:Sister chromatid exchange rate was studied in 12 early diagnosed cases of nasopharyngeal carcinoma and in their paired controls. Exchange frequencies were also analyzed in 100 healthy subjects distributed in four regions of Hunan Province and correlated to nationality, age and sex. The incidence of sister chromatid ex...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117868
更新日期：1987-09-01 00:00:00
abstract：:In a number of adverse drug reactions leading to hepatotoxicity drug metabolism is thought to be involved by generation of reactive metabolites from nontoxic drugs. In this study, an in vitro assay was developed for measurement of the impact of metabolic activation of compound on the cytotoxicity toward a human hepati...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9018-x
更新日期：2008-01-01 00:00:00
abstract：:Cerebral infarction causes severe morbidity and mortality. Most patients with cerebral infarction should take antiplatelet drugs daily, so the effects of those drugs on the regeneration of the brain need to be investigated. Aspirin and clopidogrel are the most widely used antiplatelet drugs for the prevention of ische...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9412-y
更新日期：2018-06-01 00:00:00
abstract：:This review summarizes the strategy of cellular immortalization based on the principle of targeted oncogenesis in transgenic mice, used to establish models of transimmortalized renal proximal tubule cells, referred to as PKSV-PCT and PKSV-PR-cells, and collecting duct principal cells, referred to as mpkCCD(cl4) cells....
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-006-0169-y
更新日期：2007-07-01 00:00:00
abstract：:Cholestatic liver injury, a group of diseases characterized with dysregulated bile acid (BA) homeostasis, was partly resulted from BA circulation disorders, which is commonly associated with the damage of hepatocyte barrier function. However, the underlying hepatocyte barrier-protective molecular mechanisms of cholest...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09567-9
更新日期：2021-01-05 00:00:00
abstract：:We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00755775
更新日期：1994-12-01 00:00:00
abstract：:Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal species and haemocytes of the northern quahog...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9186-6
更新日期：2011-08-01 00:00:00
abstract：:Cationic lipids are widely used for gene transfer in vitro and show promise as vectors for in vivo gene therapy applications. However, there is limited understanding of the cellular mechanisms involved in nonviral gene transfer. We investigated two major steps that could be limiting barriers to cationic lipid-mediated...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007425803756
更新日期：1998-03-01 00:00:00
