Role of undecan-2-one on ethanol-induced apoptosis in HepG2 cells.

Abstract:

:Based on the reduced expression of ethanol-oxidizing enzymes in human hepatocellular carcinoma (HepG2) cells, we analyzed the role of nonoxidative metabolites in ethanol-induced apoptosis in HepG2 cells. For this purpose, an analysis of volatile metabolites of ethanol using ion-mobility spectrometry and gas chromatography-mass spectrometry was performed. HepG2 cells exposed to 1 mmol/L ethanol exhibited significant synthesis of undecan-2-one compared to untreated cells. Undecan-2-one is a fatty acid ethyl ester metabolite synthesized through a nonoxidative pathway. Undecan-2-one had a dose-dependent cytotoxic effect on HepG2 cells as shown by release of lactate dehydrogenase (LDH). The most notable finding of this study was the potentiation of ethanol-induced apoptosis demonstrated by an increased apoptotic rate induced by undecan-2-one in ethanol-treated HepG2 cells. The data presented in this study contribute to the better understanding of the molecular mechanisms of ethanol exposure at low concentration in HepG2 cells, a human hepatocellular carcinoma-derived cell line.

journal_name

Cell Biol Toxicol

authors

Castaneda F,Zimmermann D,Nolte J,Baumbach JI

doi

10.1007/s10565-007-9009-y

subject

Has Abstract

pub_date

2007-11-01 00:00:00

pages

477-85

issue

6

eissn

0742-2091

issn

1573-6822

journal_volume

23

pub_type

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