Abstract:
:Previous studies in our laboratory indicated that immunization of male and female Wistar and Lewis rats with epididymal protein DE, resulted in the development of anti-DE antibodies in over 90% of the animals, with a significant and reversible reduction of fertility. In the present study, ELISA assays performed to analyze the evolution of the immune response indicated that antibody levels in the sera of immunized animals reached a maximum at 8 weeks after the initial injection and then gradually decreased, returning to control values by the end of the sixth month. Western blot experiments demonstrated that the immune sera specifically recognized DE in epididymal sperm extracts and epididymal cytosol, while no reaction was observed with different reproductive and essential organs. The immune sera were also capable of recognizing DE on the surface of both fresh and capacitated sperm as indicated by indirect immunofluorescence experiments. Finally, the exposure of sperm to immune sera prior to uterine insemination resulted in a significant (P < 0.05) reduction in the percentage of fertilized eggs compared to controls, with no effect on sperm motility and viability, nor on their ability to undergo capacitation. Together, these results support the participation of the raised antibodies as mediators of the antifertility effect and suggest a specific interference at the sperm-egg interaction level.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Perez Martinez S,Conesa D,Cuasnicú PSdoi
10.1016/0165-0378(95)00927-dsubject
Has Abstractpub_date
1995-05-01 00:00:00pages
31-45issue
1eissn
0165-0378issn
1872-7603pii
016503789500927Djournal_volume
29pub_type
杂志文章abstract::The present investigation was undertaken to study the association between placental apoptosis and pre-eclampsia, discriminating between pre-eclamptic pregnancies with appropriate-, and small-for-gestational-age (SGA), infants. Twenty pregnancies with pre-eclampsia and SGA (birth weight at or below -2 standard deviatio...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2003.10.001
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND:Elongation factor Tu GTP binding domain containing 2 (EFTUD2) is an alternative splicing factor that modulates cell differentiation and activation processes. EFTUD2 is known to modulate immune responses and mutation of the EFTUD2-gene lead to fetal malformation. Little is known about its expression and role ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2020.103149
更新日期:2020-08-01 00:00:00
abstract::Lymphohematopoietic cytokines play a significant role in many biological mechanisms including a number of reproductive processes such as ovulation, implantation, placentation, cervical dilation and parturition. Recent experiments have suggested that cytokines play a crucial role in the mechanisms of preterm labor and ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/s0165-0378(00)00064-4
更新日期:2000-07-01 00:00:00
abstract::A method has been developed to establish lines of transformed lymphocytes able to produce in vitro the same anti-sperm antibodies as those naturally occurring in immuno-infertile individuals. We utilized lymphocytes from a male donor whose serum contained anti-sperm antibodies of the IgG class up to the dilution 1:10,...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(95)00939-i
更新日期:1995-07-01 00:00:00
abstract::We conducted a large case-control study to assess the risk of preeclampsia with elevated sTNFp55 concentrations (markers of excessive TNF-alpha release) in Peruvian women. A total of 125 women with preeclampsia and 179 normotensive women were included in a study conducted during the period, June 1997 through January 1...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(99)00064-9
更新日期:2000-05-01 00:00:00
abstract::Decidual stromal cells (DSCs) are an important component of decidual tissues where they are in strict proximity with immune cells. Although previous research has indicated that DSCs participate in the regulation of immune cells during pregnancy, the crosstalk between DSCs and decidual NK cells (dNKs) has not been full...
journal_title:Journal of reproductive immunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jri.2015.01.004
更新日期:2015-06-01 00:00:00
abstract::The feasibility of producing epitope-specific antigens by mutation of the gene is demonstrated, the aim being to eliminate unwanted surface epitopes yet allowing the natural folding of the protein to maintain the desired epitope(s). The model protein is the beta subunit of human chorionic gonadotropin (hCG beta) which...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(96)00978-3
更新日期:1996-08-01 00:00:00
abstract::The rabbit sperm membrane autoantigen, RSA-1, has been isolated from the kidneys of bilaterally vasectomized rabbits. Using detergent extraction the IgG-RSA-1 complex in the kidney was isolated first on an anti-IgG immunocolumn. The eluted IgG-RSA-1 complex was then applied to an anti-sperm autoantibody column which b...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(84)90006-8
更新日期:1984-02-01 00:00:00
abstract::Supernatants from mixed lymphocyte reactions performed in the presence of pregnancy serum (PS) or normal human serum (NHS) were tested for interleukin-1 (IL-1) activity in a thymocyte assay. A lower response was obtained with PS-containing supernatants, suggesting that PS exerts its suppressive effect by inhibiting IL...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(88)90045-9
更新日期:1988-06-01 00:00:00
abstract::The aim of this study was to examine correlations between vaginal inflammatory cytokines (IL-1alpha, IL-1beta, IL-6 and IL-8) and pregnancy-related traits (gestational age, birth-weight, BMI, weight gain during pregnancy and vaginal pH). Differences in correlation coefficients were examined among bacterial vaginosis (...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2008.02.001
更新日期:2008-07-01 00:00:00
abstract::In our earlier study, we have reported the identification and characterization of a 57-kDa sperm membrane protein from human spermatozoa. The protein was found to be localized on the acrosome in acrosome intact spermatozoa and shifted to equatorial region after acrosomal induction. Further, we demonstrated that it pla...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2005.04.004
更新日期:2005-08-01 00:00:00
abstract::Glycodelin is a glycoprotein with 4 known glycoforms, namely glycodelin-S, glycodelin-A, glycodelin-F and glycodelin-C. The glycoforms are present in the female reproductive tract which the spermatozoa must pass through before fertilizing the oocyte. Thus the spermatozoa interact with each of these glycoforms in succe...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2009.04.012
更新日期:2009-12-01 00:00:00
abstract::Endometriosis is a prevalent gynaecological disorder with a still unclear pathogenesis. So far inflammatory mechanisms are associated with disease progression and critical reviews have discussed the so-called 'danger theory' related to endometriosis. Hence, we performed immunoassays to evaluate whether local inflammat...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2018.07.006
更新日期:2018-11-01 00:00:00
abstract::The sequence of biochemical and molecular events associated with decidualization in the primate remain unclear. In the baboon, the sequential changes during this period in vivo are characterized by the downregulation of alpha-smooth actin followed by induction of cyclooxygenase-2 (COX-2) at the implantation site and t...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/s0165-0378(01)00141-3
更新日期:2002-05-01 00:00:00
abstract::Pharmacologic inhibition of indoleamine 2,3 dioxygenase (IDO) activity during murine pregnancy results in rejection of allogeneic fetuses by the maternal immune system. Here, we show that IDO expression is restricted to perinuclear regions of primary trophoblast giant cells (TGCs) of fetal origin at mid-gestation (E10...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2003.11.003
更新日期:2004-04-01 00:00:00
abstract::Immunological abnormalities of cell-mediated and humoral immunity might be associated with the pathogenesis of endometriosis. This study has examined the effects of peritoneal fluid obtained from patients with endometriosis (ePF) on the phenotypic characteristics of macrophages and dendritic cells (DCs) derived from m...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.03.013
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare serum levels of leptin in women with unexplained infertility with fertile subjects. MATERIAL AND METHOD:Serum leptin levels of 27 infertile and 30 fertile women on day 3 of the menstrual cycle were assessed and compared in this prospective age and body mass index (BMI)...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.04.001
更新日期:2007-10-01 00:00:00
abstract::The present study has demonstrated that human extravillous trophoblast cells isolated from first trimester placentae can bind granulocyte-macrophage colony-stimulating factor (GM-CSF). We have used a technique which incorporates a phycoerythrin (PE)-conjugated GM-CSF in association with a monoclonal antibody against t...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(92)90004-n
更新日期:1992-06-01 00:00:00
abstract::The placenta forms the interface between the mother and the fetus. During placental development cytotrophoblasts differentiate to form the syncytium or to invade the decidual wall to breach maternal vessels and establish the blood flow in the intervillous space. This process is still not well understood but it is prop...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2011.01.016
更新日期:2011-04-01 00:00:00
abstract::Recurrent implantation failure (RIF) refers to cases in which women have had the failure of the embryo implantation after several in vitro fertilization (IVF). The success rate for IVF depends on many different factors. Implantation is a complex step in a successful pregnancy. Antiphospholipid antibodies (aPLs) and pl...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2020.103251
更新日期:2021-02-01 00:00:00
abstract::The mixed lymphocyte culture (MLC) of maternal and newborn (cord) cells is significantly weaker than that of father-newborn and control-newborn cultures. This hyporeactivity was found not to be due to an impaired function or tolerance of either the maternal or neonatal cells. We investigated the possibility that a spe...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(84)90041-x
更新日期:1984-01-01 00:00:00
abstract::During the past decade, accumulated evidence indicates an association between endometriosis and an alteration of humoral and cell-mediated immunity. While the role of L-carnitine in the regulation of energy metabolism is well established, it is only recently that L-carnitine has been recognized to modify the immune re...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2004.12.002
更新日期:2005-04-01 00:00:00
abstract::The objective of these experiments was to determine the effects of neonatal thymectomy on the induction of experimental autoimmune orchitis in inbred rats of the Fischer 344 and Lewis strains. It was found that thymectomy alone in Lewis rats, and thymectomy followed by immunization with testicular extract in both Lewi...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(79)90001-9
更新日期:1979-12-01 00:00:00
abstract::Galectin-1 (gal-1), a member of a family of conserved β-galactoside-binding proteins, has been shown to exert a key role during gestation. Though gal-1 is expressed at higher levels in the placenta from HELLP patients, it is still poorly understood whether systemic gal-1 levels also differ in HELLP patients. In the pr...
journal_title:Journal of reproductive immunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jri.2016.02.002
更新日期:2016-04-01 00:00:00
abstract::It has been proposed that PGE2 is an important immunosuppressant acting at the fetal-maternal interface during pregnancy. We have previously shown that horse conceptus-conditioned medium suppresses lymphocyte proliferation. This experiment was designed to determine if horse conceptus-derived immunosuppressive activity...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(92)90042-3
更新日期:1992-01-01 00:00:00
abstract::Chemokines are a new family of cytokines specialised in attracting leukocytes, acting in physiological conditions and in pathological processes. A wide variety of cell types in response to exogenous irritants or endogenous mediators of the inflammatory reaction produce them. Pivotal parts of reproductive function are ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/s0165-0378(98)00031-x
更新日期:1998-07-01 00:00:00
abstract::To analyze immunomodulating effects related to parity status, we studied trophoblast invasion grade, placental expression and systemic concentration of VEGF and its receptor Flt-1 in normal fertile (CBA/JxBALB/c) mice and abortion-prone (CBA/JxDBA/2) H-2(d)xH-2(k) mice. BALB/c or DBA/2 mated CBA/J females were, respec...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2010.03.004
更新日期:2010-06-01 00:00:00
abstract::Epstein-Barr virus (EBV)-transformed B cell lines that produce human antisperm antibodies were established using peripheral blood lymphocytes (PBLs) from infertile women with sperm immobilizing antibodies in their sera. We obtained three stable cell populations (designated B1, B2, D5) of transformed PBLs originating f...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(96)00999-0
更新日期:1996-12-01 00:00:00
abstract::The detrimental consequences of obesity on female fertility are well known, but the functional changes that occur in the ovary in response to elevated BMI are not clear. Obesity induces multiple components of a systemic inflammatory state that is a key pathway by which it initiates tissue dysfunction in adipose, liver...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2018.08.005
更新日期:2018-11-01 00:00:00
abstract::Seven monoclonal antibodies (MAs) generated against porcine zona pellucida glycoprotein, ZP3 (comprising both ZP3 alpha and ZP3 beta) were characterized for their specificities to ZP3 alpha (MA 7 and MA 28) or ZP3 beta (MA 1, MA 2, MA 10, MA 27 and MA 30) and their relative affinities in competitive ELISA. Among the s...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(93)90047-l
更新日期:1993-04-01 00:00:00