Abstract：
:Seven monoclonal antibodies (MAs) generated against porcine zona pellucida glycoprotein, ZP3 (comprising both ZP3 alpha and ZP3 beta) were characterized for their specificities to ZP3 alpha (MA 7 and MA 28) or ZP3 beta (MA 1, MA 2, MA 10, MA 27 and MA 30) and their relative affinities in competitive ELISA. Among the seven MAs tested, MA 28 showed the highest affinity for ZP3 and ZP3 alpha and MA 30 for ZP3 beta. All the antibodies bound to the zona pellucida in an indirect immunofluorescence assay, but only four (MA 7, MA 28, MA 10 and MA 30) were able to inhibit the binding of boar sperm to the porcine oocyte. Reduction followed by carboxyamidomethylation of the antigen or its chemical deglycosylation reduces reactivity to MA 7 and MA 10, suggesting that these antibodies read conformational or discontinuous determinants. The epitope recognized by MA 28 is sequential or conformational, stabilized by disulfide bonds while MA 30 reads a sequential determinant. ZP3 alpha digested with alpha-chymotrypsin, trypsin and V8 protease, respectively, revealed fragments in the range of 27-20 kDa with MA 28 in immunoblots. Proteolytic digests of ZP3 beta show that MA 30 recognizes approximately 14 kDa fragment of an alpha-chymotrypsin digest and a approximately 6 kDa fragment of a tryptic digest. These studies will help in delineation of smaller determinants of ZP involved in sperm binding.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Bagavant H,Yurewicz EC,Sacco AG,Talwar GP,Gupta SKdoi
10.1016/0165-0378(93)90047-lsubject
Has Abstractpub_date
1993-04-01 00:00:00pages
265-79issue
3eissn
0165-0378issn
1872-7603pii
0165-0378(93)90047-Ljournal_volume
23pub_type
杂志文章abstract：:In vitro fertilization (IVF) pregnancies potentially have a higher rate of preterm delivery (PTD) than do spontaneously conceived gestations, and differences persist following adjustment for multiple gestation, maternal age, and parity. The reasons for this increased susceptibility to PTD remain incompletely elucidate...
journal_title：Journal of reproductive immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.jri.2015.05.004
更新日期：2015-11-01 00:00:00
abstract：:Natural killer (NK) cells comprise of ∼70% of the immune cell population in the maternal decidua and ∼15% of the mononuclear cells in the peripheral blood. The decidual NK cells capable of producing high levels of cytokines are functionally distinct from the peripheral NK cells that exhibit high cytotoxicity. The numb...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2019.102625
更新日期：2020-02-01 00:00:00
abstract：:A high resolution two dimensional electrophoretic technique (2-D), isoelectric focusing followed by polyacrylamide gel electrophoresis, for separation of neutral and acidic human spermatozoal proteins was established. Silver stained 2-D gels revealed over 260 proteins with Mr 20-200 kDa and pI between 4.5 and 7.8. Mem...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(90)90001-m
更新日期：1990-06-01 00:00:00
abstract：PROBLEM:Placental abruption is a potential life-threatening condition for both the fetus and the mother, being significantly more common in pregnancies with male fetuses. The pathogenesis of placental abruption remains unknown. However, some recent reports point toward a maternal immune response against the fetus as a ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2007.03.003
更新日期：2007-08-01 00:00:00
abstract：:The proportion of stromal cells expressing DR antigens of the HLA system or reacting with an anti-macrophage antibody was studied by immunohistochemical methods in human proliferative, secretory and post-menopausal endometria. DR+ cells showed a rounded morphology with short expansions and represented 13-25% of the st...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(90)90015-x
更新日期：1990-09-01 00:00:00
abstract：:The rabbit sperm membrane autoantigen, RSA-1, has been isolated from the kidneys of bilaterally vasectomized rabbits. Using detergent extraction the IgG-RSA-1 complex in the kidney was isolated first on an anti-IgG immunocolumn. The eluted IgG-RSA-1 complex was then applied to an anti-sperm autoantibody column which b...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(84)90006-8
更新日期：1984-02-01 00:00:00
abstract：:An iso-antigen has been isolated from tritium-labelled human spermatozoa by detergent solubilization, lectin affinity chromatography and indirect immunoprecipitation using human iso-antisera. Isolated material was characterized by SDS-polyacrylamide gel electrophoresis. With these iso-antisera it was possible to isola...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(83)90020-7
更新日期：1983-01-01 00:00:00
abstract：:A large clinical literature suggests that estradiol (E(2)) plays a critical role in immune function. To further explore the relationship between E(2) and immune function, we examined a variety of immunological parameters in a rhesus monkey model of menopause and hormone replacement therapy. Rhesus monkeys (Age, 13.7+/...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(00)00087-5
更新日期：2001-04-01 00:00:00
abstract：AIM:Th17 cells are a newly discovered T helper lymphocyte subpopulation, producing interleukin IL-17. Th17 cells are present in blood and peritoneal fluid (PF) at different stages of endometriosis. We aim to establish their potential importance in the pathogenesis and clinical features of the disease. METHODS:The perc...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2016.04.289
更新日期：2016-09-01 00:00:00
abstract：:We have previously shown that DBA/1 mice immunized with heterologous type II collagen showed remission of the subsequent collagen-induced arthritis (CIA) when pregnant, but experienced exacerbation postpartum. Measurement of anticollagen antibody (aCa) responses by ELISA in primiparous mice immunized at day 1 of pregn...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(88)90007-1
更新日期：1988-08-01 00:00:00
abstract：:Chemokines are a new family of cytokines specialised in attracting leukocytes, acting in physiological conditions and in pathological processes. A wide variety of cell types in response to exogenous irritants or endogenous mediators of the inflammatory reaction produce them. Pivotal parts of reproductive function are ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-0378(98)00031-x
更新日期：1998-07-01 00:00:00
abstract：:A large number of cells in the first trimester human placenta have been shown to express leucocyte-common antigen by the use of a monoclonal antibody in an immunoperoxidase technique. The distribution of endometrial granulocytes in the decidua of early pregnancy indicates that a large proportion of these cells bear th...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(83)90247-4
更新日期：1983-11-01 00:00:00
abstract：:Monoclonal antibodies (MAb) were raised against a testicular membrane fraction from 18-day post coitum (p.c.) rat testes. One antibody, designated 4B6.3E10 (mu, kappa), was obtained which specifically reacted with gonocytes in the fetal testis. No significant cross-reactivity with other tissues from the 18-day p.c. em...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(93)90030-l
更新日期：1993-01-01 00:00:00
abstract：:A successful pregnancy is characterised by an increase in Th2 cytokines and suppression of Th1 cytokine production. A Th1 to Th2 cytokine shift is also observed in the disease progression of HIV infection. Highly active antiretroviral therapy (HAART) suppresses HIV viremia, increases CD4+ cell counts and counteracts t...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2005.12.001
更新日期：2006-06-01 00:00:00
abstract：:For some time, reproductive immunologists have worked to understand the balance between maternal tolerance of the fetus, maternal health, and fetal protection which leads to successful pregnancy in mammalian species. We have always understood the potential importance of multiple factors, including nutrition, genetics,...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2017.09.005
更新日期：2017-09-01 00:00:00
abstract：PROBLEM:Markers of maternal inflammation may determine infant birth outcomes. METHOD OF STUDY:Maternal serum samples were collected at 28 weeks gestation (n = 1418) in the Seychelles Child Development Study Nutrition Cohort 2 and analyzed for immune markers by MSD multiplex assay, including cytokines from the Th1 (IFN...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2019.102623
更新日期：2020-02-01 00:00:00
abstract：:Alloimmunization therapy using the partner's leukocytes has been reported to be effective in preventing the failure of pregnancy in patients who have suffered RSA of unknown cause. After alloimmunization, several investigators have reported the presence of blocking factors (BF) in women with successful pregnancies in ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(99)00021-2
更新日期：1999-09-01 00:00:00
abstract：:Testicular macrophages (TMs) are important contributors to the response of the testis to infection, as well as the regulation of spermatogenesis, steroidogenesis and other homeostatic functions of the testis. The TMs are the largest population of immune cells in a region of tight immunoregulation, where both innate an...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2013.01.001
更新日期：2013-04-01 00:00:00
abstract：:The sequence of biochemical and molecular events associated with decidualization in the primate remain unclear. In the baboon, the sequential changes during this period in vivo are characterized by the downregulation of alpha-smooth actin followed by induction of cyclooxygenase-2 (COX-2) at the implantation site and t...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-0378(01)00141-3
更新日期：2002-05-01 00:00:00
abstract：:Controversy exists as to whether active immunotherapy with allogeneic lymphocyte transfusions (ALT) or passive immunotherapy with intravenous immunoglobulin (IvIg) improve the chance of live birth in women with unexplained recurrent miscarriages (RM). Meta-analyses of the placebo-controlled trials carried out as Cochr...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2003.09.003
更新日期：2004-06-01 00:00:00
abstract：:During a pregnancy, the mother accepts her semi-allogeneic fetus with no signs of immunological rejection. Therefore, some modulation of the maternal immune system must occur. Similarly, changes in the host's immune system occurs during infections with parasites. In a study conducted in an endemic area in Bolivia, it ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2019.08.001
更新日期：2019-11-01 00:00:00
abstract：:Recent discoveries suggest that T-regulatory lymphocytes (Treg) might play an important role in the pathophysiology of preterm labor. The aim of this study was to assess the relationship among the levels of maternal circulating Treg cells, uterine cervical length, and the risk of preterm labor. Sixty women with regula...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2014.04.001
更新日期：2014-12-01 00:00:00
abstract：:Tolerance to the developing fetus is thought to be accomplished through the action of several molecules that are able to modulate the maternal immune response. Among several mechanisms involved in pregnancy maintenance, progesterone-induced immunomodulation, asymmetric antibody (AAb) production, indoleamine 2,3-dioxyg...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2008.11.002
更新日期：2009-01-01 00:00:00
abstract：:Currently, different approaches are used to select oocytes for in vitro fertilization (IVF) procedures, but they do not assure a significant association with the pregnancy outcome. Since several studies have proposed the expression of HLA-G antigens in early embryos to be a possible marker of elevated implantation rat...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2007.02.005
更新日期：2007-06-01 00:00:00
abstract：:A quantitative immunohistochemical study of IgA-containing plasma cells in the human uterine cervix has shown that the number of such cells increases during the late secretory phase of the menstrual cycle but decreases in the early secretory phase of the cycle, in pregnancy, in cystic glandular hyperplasia of the endo...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(82)90020-1
更新日期：1982-02-01 00:00:00
abstract：:The tissue distribution, molecular weight, and biochemical nature of an antigen detected by a mouse monoclonal antibody designated 283D3 and raised against rabbit visceral yolk sac endodermal cells, has been investigated. The antigen is located on the luminal side of apical tubules and large sub-apical vesicles in rab...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(93)90046-k
更新日期：1993-04-01 00:00:00
abstract：:The placenta forms the interface between the mother and the fetus. During placental development cytotrophoblasts differentiate to form the syncytium or to invade the decidual wall to breach maternal vessels and establish the blood flow in the intervillous space. This process is still not well understood but it is prop...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2011.01.016
更新日期：2011-04-01 00:00:00
abstract：:Secretory leukocyte protease inhibitor (SLPI) and progranulin (PGRN) are secretory proteins with an anti-inflammatory property. Their involvement in cervical remodeling in pregnant uterus is not yet elucidated. Thus, this study aimed to explore the significance of SLPI and PGRN in the maintenance of pregnancy by inves...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2020.103241
更新日期：2021-02-01 00:00:00
abstract：:Alloreactive T levels of para-aortic lymph nodes (PALN) and spleen were determined on different days of pregnancy in BALB/c females by local and systemic graft-versus-host (GvH) assays. A significant increase in GvH reactivity was registered early in both allogeneic and syngeneic matings, operating not only towards pa...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(85)90036-1
更新日期：1985-11-01 00:00:00
abstract：:The state of pregnancy is an immunological enigma during which the body must prevent rejection of the antigenically foreign fetus while at the same time maintain sufficient maternal host defense mechanisms to combat infection. Although most studies on the immunology of pregnancy focus on immune suppression, several st...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(98)00076-x
更新日期：1998-11-01 00:00:00
