Partial molecular characterization of Sm8, a tegumental antigen of Schistosoma mansoni.

abstract：:Coccidian oocysts containing 16 sporocysts with 4 sporozoites in each were observed in a faecal sample from Sclerurus scansor collected in the Itatiaia National Park, southeastern region of Brazil. The oocysts are characterized by ellipsoidal shape measuring 42.5 x 32.8 mm, with smooth, thick double-layered wall of a ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Kawazoe U,Gouvêa H

更新日期：1999-03-01 00:00:00

abstract：:This report describes the isolation of a Leishmania chagasi strain from a bat (Carollia perspicillata), and its identification using biological methods and molecular characterization. The parasites were isolated in an artificial culture medium from a blood sample extracted from a bat heart. The isolate was then inocul...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： De Lima H,Rodríguez N,Barrios MA,Avila A,Cañizales I,Gutiérrez S

更新日期：2008-06-01 00:00:00

abstract：:This study investigated the rate of human papillomavirus (HPV) persistence, associated risk factors, and predictors of cytological alteration outcomes in a cohort of human immunodeficiency virus-infected pregnant women over an 18-month period. HPV was typed through L1 gene sequencing in cervical smears collected durin...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Meyrelles AR,Siqueira JD,Santos PP,Hofer CB,Luiz RR,Seuánez HN,Almeida G,Soares MA,Soares EA,Machado ES

更新日期：2016-02-01 00:00:00

abstract：:A study of crepuscular and night-biting mosquitoes was conducted at remote settlements along the Padauiri River, middle Negro River, state of Amazonas, Brazil. Collections were performed with human bait and a CDC-light trap on three consecutive days per month from June 2003-May 2004. In total, 1,203 h of collection we...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Suárez-Mutis MC,Fé NF,Alecrim W,Coura JR

更新日期：2009-02-01 00:00:00

abstract：:In a previous study, the Schistosoma mansoni Rho1 protein was able to complement Rho1 null mutant Saccharomyces cerevisiae cells at restrictive temperatures and under osmotic stress (low calcium concentration) better than the human homologue (RhoA). It is known that under osmotic stress, the S. cerevisiae Rho1 trigger...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Aguiar PH,Santos DN,Lobo FP,Santos TM,Macedo AM,Pena SD,Machado CR,Franco GR

更新日期：2006-09-01 00:00:00

abstract：:Corynebacterium striatum is a potentially pathogenic microorganism with the ability to produce outbreaks of nosocomial infections. Here, we document a nosocomial outbreak caused by multidrug-resistant (MDR) C. striatum in Rio de Janeiro, Brazil. C. striatum identification was confirmed by 16S rRNA and rpoB gene sequen...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Baio PV,Mota HF,Freitas AD,Gomes DL,Ramos JN,Sant'Anna LO,Souza MC,Camello TC,Hirata Junior R,Vieira VV,Mattos-Guaraldi AL

更新日期：2013-02-01 00:00:00

abstract：:In an endemic area of cutaneous leishmaniasis in Rio de Janeiro State where a mule had been found infected, a systematic search among equines was performed, resulting in the detection of Leishmania parasites in skin lesions of 30.8% of the animals, which included horses and mules. The eventual role of equines in the e...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Aguilar CM,Rangel EF,Deane LM

更新日期：1986-10-01 00:00:00

abstract：:In this transversal study, factors related to infection with and transmission of Schistosoma mansoni were explored. Based on stool examinations of two Kato-Katz smears of a single sample, the prevalences of schistosomiasis and geohelminths were established. In a multivariable analysis, sets of demographic, socio-econo...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Enk MJ,Lima AC,Barros Hda S,Massara CL,Coelho PM,Schall VT

更新日期：2010-07-01 00:00:00

abstract：:We investigated the cytokine profile of peripheral mononuclear cells from chronic osteomyelitis (OST) patients following in vitro stimulation with staphylococcal enterotoxin A (SEA). We demonstrate that stimulation with SEA induced prominent lymphocyte proliferation and high levels of tumour necrosis factor (TNF)-α, i...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ferreira GF,Moraes C,Silveira AM,Correa-Oliveira R,Teixeira-Carvalho A,Martins-Filho OA,Moreno EC,do Carmo LS,Fraga LA,Malaquias LC

更新日期：2012-05-01 00:00:00

abstract：:Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from an infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzym...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Schechter M

更新日期：1987-01-01 00:00:00

abstract：:The first steps in leishmaniasis are critical in determining the evolution of the disease. Major advances have recently been done in understanding this crucial moment. Fundamental research in parasite-vector interaction, parasite biology, insect saliva, and vertebrate host response have shed new light and uncovered a ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： de Almeida MC,Vilhena V,Barral A,Barral-Netto M

更新日期：2003-10-01 00:00:00

abstract：:Acute cases of schistosomiasis have been found on the coastal area of Pernambuco, Brazil, due to environmental disturbances and disorderly occupation of the urban areas. This study identifies and spatially marks the main foci of the snail host species, Biomphalaria glabrata on Itamaracá Island. The chaotic occupation ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Barbosa CS,Araújo KC,Antunes L,Favre T,Pieri OS

更新日期：2004-01-01 00:00:00

abstract：:Prosorhynchoides arcuatus (Linton, 1900) from the intestine of Pomatomus saltator (L.) from the Atlantic coast of the State of Rio de Janeiro is studied by scanning electron microscopy, with detailed description of tegumental spines. Comments on the synonymy of this species with Bucephalopsis callicotyle Kohn, 1962 ar...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cohen SC,Kohn A,Barth OM

更新日期：1995-01-01 00:00:00

abstract：:Triatoma sordida is a peridomestic Triatominae that could play an important role in the transmission of Trypanosoma cruzi, although its vectorial competence is not well known. The aim of this work was to evaluate two aspects of the vectorial competence: the feeding behaviour and defaecation patterns, and to compare th...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Crocco LB,Catalá SS

更新日期：1996-07-01 00:00:00

abstract：:Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Strelkova MV,Pacheco RS,Bulat SA,Rakitskaya TA

更新日期：1997-01-01 00:00:00

abstract：:Lutzomyia evandroi Costa Lima and Antunes, 1936 is found in Rio Grande do Norte, northeastern Brazil, in areas of visceral and mucocutaneous leishmaniasis and follows the same geographic distribution of L. longipalpis. The biological cycle, oviposition, morphological and behavioral characteristics of the species were ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： de Melo Ximenes Mde F,Maciel JC,Jerônimo SM

更新日期：2001-08-01 00:00:00

abstract：BACKGROUND:Lutzomyia umbratilis, the vector for Leishmania guyanensis in northern South America, has been found naturally infected with L. guyanensis only in areas north of the Negro and Amazon rivers. While populations of this sand fly species are also found in areas south of these rivers, these populations have never...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Soares RP,Nogueira PM,Secundino NF,Marialva EF,Ríos-Velásquez CM,Pessoa FAC

更新日期：2018-03-01 00:00:00

abstract：:Schistosoma mansoni is one of the three main causative agents of human schistosomiasis, a major health problem with a vast socio-economic impact. Recent advances in the proteomic analysis of schistosomes have revealed that peptidases are the main virulence factors involved in the pathogenesis of this disease. In this ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Silva LL,Marcet-Houben M,Zerlotini A,Gabaldón T,Oliveira G,Nahum LA

更新日期：2011-11-01 00:00:00

abstract：:We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Tang TH,López-Vélez R,Lanza M,Shelley AJ,Rubio JM,Luz SL

更新日期：2010-09-01 00:00:00

abstract：:The aim of the present study was to examine the parasite fauna present in rodent coprolites collected from Cueva Huenul 1 (CH1), northern Neuquén (Patagonia, Argentina), an archaeological site that provides stratified sequences of archaeological and palaeontological remains dating from the Late Pleistocene/Early Holoc...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Beltrame MO,Sardella NH,Fugassa MH,Barberena R

更新日期：2012-08-01 00:00:00

abstract：:Five field populations of Musca domestica L. collected in poultry farms were bioassayed in order to detect possible resistance to the larvicide cyromazine in Brazil. The concentrations used were 0, 0.5, 0.1, 0.2, 0.4, 1, 2, 4 and 8 ppm. Three populations (Petrópolis, RJ, Montes Claros, MG and Promissão, SP) were resis...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Pinto MC,do Prado AP

更新日期：2001-07-01 00:00:00

abstract：:Preparations of simian rotavirus SA11 maintained in different laboratories were compared with each other by polyacrylamide gel electrophoresis of genomic RNA. Differences in the migration of genome segments 4, 5 and 7 allowed the classification of eight virus preparations into four electrophoretic types. ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Pereira HG,Gouvea VS,Fialho AM

更新日期：1986-10-01 00:00:00

abstract：:To study the action of molluscicide nine ponds were selected: 3 of them lying in Maruim municipality, 29 km far from north Aracaju, the State capital, and 6 ponds in Itabaianinha municipality, 118 km far from south Aracaju. This study was carried out for 16 months. Environmental parameters observed were those thought ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Rosas E,Ribeiro EC

更新日期：1987-01-01 00:00:00

abstract：:We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequences. ESTs can be used to identify genes on the basis of their homology with sequences from other species deposit...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Franco GR,Simpson AJ,Pena SD

更新日期：1995-03-01 00:00:00

abstract：:Molecular characterization of one stable strain of Trypanosoma cruzi, the 21 SF, representative of the pattern of strains isolated from the endemic area of São Felipe, State of Bahia, Brazil, maintained for 15 years in laboratory by serial passages in mice and classified as biodeme Type II and zymodeme 2 has been inve...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Campos RF,Gonçalves MS,dos Reis EA,dos Reis MG,Andrade SG

更新日期：1999-01-01 00:00:00

abstract：:Cytokines are important in the cell-mediated response to Schistosoma mansoni eggs. We have found that Th2 cytokine responses (e.g. IL-4 and IL-5) are augmented after egg laying begins while Th1 responses (IL-2 and IFN-gamma) are down regulated in S. mansoni infected mice. Treatment of mice with anti-IL-5 monoclonal an...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cheever AW,Xu Y,Macedonia JG,Cox T,Hieny S,Sher A

更新日期：1992-01-01 00:00:00

abstract：:Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypa...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Penha LL,Hoffmann L,Souza SS,Martins AC,Bottaro T,Prosdocimi F,Faffe DS,Motta MC,Ürményi TP,Silva R

更新日期：2016-11-01 00:00:00

abstract：:This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument. Primers targeting the gene encoding the SSU rRNA were designed to amplify with high specificity DNA from Schistosoma mansoni, in a real time quantitative PCR ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Gomes AL,Melo FL,Werkhauser RP,Abath FG

更新日期：2006-09-01 00:00:00

abstract：BACKGROUND:The time of progression towards AIDS can vary greatly among seropositive patients, and may be associated with host genetic variation. The NR1I2 (PXR) gene, a ligand-activated transcription factor, regulates the transcription immune pathway genes and can therefore be targets of viral replication mechanisms in...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Medeiros RM,Menti CF,Benelli JL,Matte MC,Melo MG,Almeida SE,Fiegenbaum M

更新日期：2017-04-01 00:00:00

abstract：:The leishmanicidal activity of four batches of meglumine antimoniate, produced in Farmanguinhos-Fiocruz, Brazil (TAMs), was assessed and compared to Glucantime-Aventis Pharma Ltda. Using the amastigote-like in vitro model, the active concentrations of Sb v varied from 10microg/ml to 300microg/ml for L. (L.) chagasi an...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Morais-Teixeira Ed,Carvalho AS,Costa JC,Duarte SL,Mendonça JS,Boechat N,Rabello A

更新日期：2008-06-01 00:00:00