Diversity among French Bacillus anthracis isolates.

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00

abstract：:Although rotavirus genotype P[6] is one of the three most common VP4 specificities associated with human infection, the relatively few sequence data available in public databases suggest that the genetic variability within P[6] might be presently unexplored. Thus far, two human P[6] lineages (M37-like and AU19-like) a...

journal_title：Journal of clinical microbiology

authors： Bányai K,Martella V,Jakab F,Melegh B,Szücs G

更新日期：2004-09-01 00:00:00

abstract：:This case report describes the fungal dermatitis caused by Neosartorya hiratsukae infection in a household hedgehog confirmed by microscopic examination of conidiophores and DNA analyses including the internal transcribed spacer region, partial beta-tubulin, and the calmodulin gene. It is the first report of a natural...

journal_title：Journal of clinical microbiology

authors： Han JI,Na KJ

更新日期：2008-09-01 00:00:00

abstract：:The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma samples was characterized for five patients at the baseline and during the first week of antiretroviral therapy by two c...

journal_title：Journal of clinical microbiology

authors： Tang YW,Huong JT,Lloyd RM Jr,Spearman P,Haas DW

更新日期：2000-12-01 00:00:00

abstract：:Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection by conventional cell culture. The sensitivity and specificity of the suspension-infection technique compared with those of conventional culture were 95.9 to 98.2% and 97.5 to 100%, respectively, de...

journal_title：Journal of clinical microbiology

authors： Luker G,Chow C,Richards DF,Johnson FB

更新日期：1991-07-01 00:00:00

abstract：:Strains of Candida albicans obtained from human immunodeficiency virus (HIV)-positive individuals prior to their first episode of oral thrush were already in a high-frequency mode of switching and were far more resistant to a number of antifungal drugs than commensal isolates from healthy individuals. Switching in the...

journal_title：Journal of clinical microbiology

authors： Vargas K,Messer SA,Pfaller M,Lockhart SR,Stapleton JT,Hellstein J,Soll DR

更新日期：2000-10-01 00:00:00

abstract：:We have developed a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis. For assay development, 23 drug-resistant isolates of M. tuberculosis having 29 different mu...

journal_title：Journal of clinical microbiology

authors： Ong DC,Yam WC,Siu GK,Lee AS

更新日期：2010-04-01 00:00:00

abstract：:Variability of disease manifestations has been noted in patients with Lyme disease. A contributing factor to this variation may be the number of spirochetes present in infected patients. We evaluated clinical and laboratory findings for patients with erythema migrans with regard to the number of Borrelia burgdorferi o...

journal_title：Journal of clinical microbiology

authors： Liveris D,Wang G,Girao G,Byrne DW,Nowakowski J,McKenna D,Nadelman R,Wormser GP,Schwartz I

更新日期：2002-04-01 00:00:00

abstract：:Genetic determinants of the invasive phenotype of Shigella spp. and enteroinvasive Escherichia coli (EIEC), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. Several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate t...

journal_title：Journal of clinical microbiology

authors： Venkatesan M,Buysse JM,Vandendries E,Kopecko DJ

更新日期：1988-02-01 00:00:00

abstract：:Vibrio cholerae serotype O1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the United States. Although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir o...

journal_title：Journal of clinical microbiology

authors： Johnston JM,McFarland LM,Bradford HB,Caraway CT

更新日期：1983-05-01 00:00:00

abstract：:Moxalactam is highly hydrolyzed by plasmid-mediated metallo-β-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing...

journal_title：Journal of clinical microbiology

authors： Pluquet E,Arlet G,Bingen E,Drieux L,Mammeri H

更新日期：2011-07-01 00:00:00

abstract：:In vitro susceptibility patterns of newer beta-lactamase-inhibiting antibiotics ampicillin-sulbactam (A/S) and amoxicillin-clavulanic acid (A/C) for 100 consecutive isolates of Acinetobacter baumannii obtained from various clinical samples were studied. The A/C MIC for 86% of the strains was more than 16/8 microgram/m...

journal_title：Journal of clinical microbiology

authors： Pandey A,Kapil A,Sood S,Goel V,Das B,Seth P

更新日期：1998-11-01 00:00:00

abstract：:Strains of the Beijing genotype family of Mycobacterium tuberculosis are a cause of particular concern because of their increasing dissemination in the world and their association with drug resistance. Phylogenetically, this family includes distinct ancient and modern sublineages. The modern strains, contrary to the a...

journal_title：Journal of clinical microbiology

authors： Ribeiro SC,Gomes LL,Amaral EP,Andrade MR,Almeida FM,Rezende AL,Lanes VR,Carvalho EC,Suffys PN,Mokrousov I,Lasunskaia EB

更新日期：2014-07-01 00:00:00

abstract：:We standardized and assessed the performance of an in-house microtiter assay for determining the susceptibilities of Mycobacterium tuberculosis clinical isolates to isoniazid based on mycobacteriophage amplification technology. Seventy isolates (43 resistant and 27 sensitive according to the BACTEC 460 radiometric met...

journal_title：Journal of clinical microbiology

authors： Galí N,Domínguez J,Blanco S,Prat C,Alcaide F,Coll P,Ausina V

更新日期：2006-01-01 00:00:00

abstract：:Bilophila wadsworthia is a common inhabitant of the human colon and has been associated with appendicitis and other local sites of inflammation in humans. Challenge-exposure or prevalence studies in laboratory and other animals have not been reported. B. wadsworthia is closely related phylogenetically to Desulfovibrio...

journal_title：Journal of clinical microbiology

authors： McOrist AL,Warhurst M,McOrist S,Bird AR

更新日期：2001-04-01 00:00:00

abstract：:A panel of new cytomegalovirus (CMV) assays for use on the Architect instrument has been developed, including a CMV avidity assay based on a new technology. The purpose of this study was to compare the performance characteristics of the fully automated CMV immunoglobulin M (IgM), IgG, and IgG avidity tests on the Arch...

journal_title：Journal of clinical microbiology

authors： Lagrou K,Bodeus M,Van Ranst M,Goubau P

更新日期：2009-06-01 00:00:00

abstract：:A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently de...

journal_title：Journal of clinical microbiology

authors： Kim W,Park HK,Hwang WJ,Shin HS

更新日期：2013-03-01 00:00:00

abstract：:An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary pathways of the isolate. Nucleotide and phylogenetic analyses of part of the VP1 genomic region reveal...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulou Z,Dedepsidis E,Pliaka V,Tsakogiannis D,Pratti A,Levidiotou-Stefanou S,Markoulatos P

更新日期：2010-05-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nu...

journal_title：Journal of clinical microbiology

authors： Deback C,Boutolleau D,Depienne C,Luyt CE,Bonnafous P,Gautheret-Dejean A,Garrigue I,Agut H

更新日期：2009-03-01 00:00:00

abstract：:Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...

journal_title：Journal of clinical microbiology

authors： Chagla AH,Borczyk AA,Facklam RR,Lovgren M

更新日期：1998-08-01 00:00:00

abstract：:Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

journal_title：Journal of clinical microbiology

authors： Vargas KG,Joly S

更新日期：2002-02-01 00:00:00

abstract：:Lyme disease prevails as the most commonly transmitted tick-borne infection in the United States, and serologic evaluation for antibodies to Borrelia burgdorferi remains the recommended modality for diagnosis. This review presents a brief historical perspective on the evolution of serologic assays for Lyme disease and...

journal_title：Journal of clinical microbiology

authors： Theel ES

更新日期：2016-05-01 00:00:00

abstract：:Bartonella henselae or Bartonella elizabethae DNA from EDTA-anticoagulated blood samples obtained from four dogs was amplified and sequenced. The results showed that B. elizabethae should be added to the list of Bartonella species (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are current...

journal_title：Journal of clinical microbiology

authors： Mexas AM,Hancock SI,Breitschwerdt EB

更新日期：2002-12-01 00:00:00

abstract：:Automation of the clinical microbiology laboratory has become more prominent as laboratories face higher specimen volumes and understaffing and are becoming more consolidated. One recent advancement is the use of digital image analysis to rapidly distinguish between chromogenic growth for screening bacterial cultures....

journal_title：Journal of clinical microbiology

authors： Faron ML,Buchan BW,Relich RF,Clark J,Ledeboer NA

更新日期：2020-03-25 00:00:00

abstract：:In contrast to cervical cancer, integration of human papillomavirus (HPV) DNA into the host genome has been considered a rare event in cancer precursor lesions (cervical intraepithelial neoplasia [CIN]). With our new real-time PCR method, we demonstrated that integrated HPV type 16 (HPV16) is already present in CIN le...

journal_title：Journal of clinical microbiology

authors： Peitsaro P,Johansson B,Syrjänen S

更新日期：2002-03-01 00:00:00

abstract：:Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. Th...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Weyant RS,Steigerwalt AG,Moss CW,Hollis DG,Weaver RE,Ashford D,Brenner DJ

更新日期：1995-01-01 00:00:00

abstract：:Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variation in RSV RNA load ...

journal_title：Journal of clinical microbiology

authors： van de Pol AC,Wolfs TF,van Loon AM,Tacke CE,Viveen MC,Jansen NJ,Kimpen JL,Rossen JW,Coenjaerts FE

更新日期：2010-10-01 00:00:00

abstract：:We validated a commercial enzyme-linked immunosorbent assay for the detection of anti-CagA antibodies in Brazilian patients with Helicobacter pylori infection. The test presented high sensitivity (97.4%) and specificity (88.9%) when employed in patients without gastric carcinoma. However, in gastric carcinoma patients...

journal_title：Journal of clinical microbiology

authors： Rocha AM,Rocha GA,Santos A,de Oliveira CA,Queiroz DM

更新日期：2003-01-01 00:00:00

abstract：:Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. T...

journal_title：Journal of clinical microbiology

authors： Giacomelli LR,Helbel C,Ogassawara RL,Barreto AM,Martins FM,Cardoso CL,Leite CQ

更新日期：2005-08-01 00:00:00

abstract：:The API 20S system (Analytab Products, Plainview, N.Y.) was evaluated for the rapid identification of multiply antibiotic-resistant aerobic diphtheroids. Sixty-eight clinical isolates of multiply resistant Centers for Disease Control group JK and group D2 corynebacteria had API 20S profiles which were clearly differen...

journal_title：Journal of clinical microbiology

authors： Kelly MC,Smith ID,Anstey RJ,Thornley JH,Rennie RP

更新日期：1984-02-01 00:00:00