Detection and characterization of membrane-bound selenoproteins in the kidney of the rat.

Abstract:

:The essential trace element selenium is of fundamental importance to human biology. Information on the membrane-bound selenoproteins was obtained by labeling of rats in vivo with (75)Se-selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS-PAGE, two-dimensional electrophoresis, and selenium detection by autoradiography. In this way, in addition to already known membrane-bound selenoproteins three novel selenoproteins in the molecular mass range of 38-40, 18, and 12-13 kDa were detected in the microsomal membranes.

journal_name

Ann N Y Acad Sci

authors

Kyriakopoulos A,Bertelsmann H,Graebert A,Hoppe B,Kuhbacher M,Behne D

doi

10.1111/j.1749-6632.2002.tb04652.x

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

289-92

eissn

0077-8923

issn

1749-6632

journal_volume

973

pub_type

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