Abstract:
:Recent in vivo studies suggest a role for relaxin-3 in feeding and stress. To further elucidate the function of relaxin-3 in the central nervous system, we have employed a complementary approach, based on RNA interference, to modulate relaxin-3 expression. We have designed, constructed, and characterized three microRNAs (miRNAs) targeting different regions of the relaxin-3 transcript. These were tested to determine the amount of miRNA required to achieve the greatest knockdown and for their ability to reduce the expression of relaxin-3 in transfected HEK293Tcells. All miRNA constructs significantly reduced relaxin-3-induced cAMP responses; however, miR499 was most effective. This should be a useful tool for in vitro studies and central targeting of relaxin-3 in vivo using viral delivery systems.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Callander GE,Thomas WG,Bathgate RAdoi
10.1111/j.1749-6632.2008.03816.xsubject
Has Abstractpub_date
2009-04-01 00:00:00pages
261-4eissn
0077-8923issn
1749-6632pii
NYAS03816journal_volume
1160pub_type
杂志文章abstract::Most of the plasma suppressive activity was associated with alpha 2M in both normal subjects and cancer patients. alpha 2M binding to physiological levels of proteases was associated with an increase in the ability to suppress lymphocyte reactivity in the TEEM test. alpha 2M binding to proteases released a peptide tha...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1983.tb18131.x
更新日期:1983-01-01 00:00:00
abstract::There are multiple lines of compelling evidence supporting the beneficial effect of exercise on the prevention and/or improvement of certain chronic diseases. However, exhaustive or intense exercise causes oxygen free radical generation and oxidative stress, which can lead to injuries and chronic fatigue as well as in...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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abstract::In epithelial and endothelial cell layers tight junctions form selective apicolateral paracellular barriers separating luminal and extracellular spaces from the underlying tissues. Within the tight junctions the tetraspan transmembrane proteins occludin, claudins, and tricellulin form anastomosing strands of protein c...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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abstract::Both hereditary and environmental factors influence the risk of cancer. Four risk categories, or oncodemes, can exist for a particular kind of cancer, depending upon the presence of neither, one, or both factors: (1) spontaneous, or background; (2) hereditary; (3) environmental; (4) interactive. In the second, mutatio...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1997.tb48593.x
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abstract::Significant advances have recently been made in understanding the regulation of Ca(2+)-ATPase by phospholamban and in modeling their structures. However, these insights would be furthered by determining the 3-D structure of both proteins within the membrane, thus revealing the structural basis for their interaction. T...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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abstract::tRNA prepared from cells of E. coli B that had been incubated with 0.5% DL-ethionine (Ethio sRNA) was found to accept methyl groups from 14CH3-S-adenosyl-methionine in the enzymatic reaction catalyzed in vitro by tRNA methyl transferases from untreated cells of the same organism. tRNA from cells that were not exposed ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:1975-08-08 00:00:00
abstract::This paper presents a review of the publicly available knowledge base concerning turbine blade tip heat transfer, from the early fundamental research which laid the foundations of our knowledge, to current experimental and numerical studies utilizing engine-scaled blade cascades and turbine rigs. Focus is placed on hi...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:2011-12-01 00:00:00
abstract::A type of unit discharge, termed "luxotonic," has been found in the striate cortex of unanesthetized squirrel monkeys and macaques.6, 21 The firing frequency of these units shows relatively little adaptation, continues indefinitely (hours), and reflects the level of diffuse illumination of the eye. The more numerous "...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1977.tb39723.x
更新日期:1977-01-01 00:00:00
abstract::1. The flow divider of the brachiocephalic branching of the rabbit aorta had both high and low shear stress regions, each of which was covered by endothelial cells with low and high permeability respectively, even in normolipidemic intact rabbits. When rabbits were placed on an atherogenic diet, low shear regions were...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1994.tb17311.x
更新日期:1995-01-17 00:00:00
abstract::The study of the morphological phenotypes in patients with different mutations of the PMP22 gene gives additional insights into the role of the protein in myelin function. The pathology in young patients is in some aspects different from the pathology in older patients, providing essential and additional information a...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
abstract::We reevaluated the exchange stoichiometry of the Na(+)/Ca(2+) exchange current by measuring its reversal potential. The exchange current was measured from the inside-out macropatch excised from intact sarcolemma of guinea pig ventricular myocytes. This method provides more accurate control of extracellular and cytopla...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2002.tb04730.x
更新日期:2002-11-01 00:00:00
abstract::The kinetics of the E(2) --> E(1) conformational change of unphosphorylated Na(+),K(+)-ATPase from rabbit kidney were investigated via the stopped-flow technique using the fluorescent label RH421 (pH 7.4, 24 degrees C). The enzyme was preequilibrated in a solution containing 25 mM histidine and 0.1 mM EDTA to initiall...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2003.tb07154.x
更新日期:2003-04-01 00:00:00
abstract::Normal human mitotic cells do not proliferate indefinitely in culture but undergo a limited number of divisions and progressively reach a state of irreversible growth arrest, a process termed replicative senescence. Hypoxia is a situation of reduced oxygen concentration that relates to many physiological and pathophys...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1404.025
更新日期:2007-11-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:1992-11-30 00:00:00
abstract::The rapid (IKr) and slow (IKs) delayed rectifier K+ currents are key regulators of cardiac repolarization. HERG encodes the Kr channel, and KVLQT1 and hminK encode subunits that coassemble to form Ks channels. Mutations in any one of these genes cause Romano-Ward syndrome, an autosomal dominant form of long QT syndrom...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:1999-04-30 00:00:00
abstract::Sixty years ago Hans Selye discovered that the neuroendocrine and immune systems interact during stress. The pathophysiological significance of neuroendocrine-immune interaction during injury has only been recognized recently. Today it is rapidly emerging that, in addition to defense against exogenous pathogenic agent...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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abstract::The recording of chemosensory event-related potentials (CSERPs) has been established as an objective method in the assessment of central odor processing in humans. In the present study CSERPs were used to investigate whether human body odor is genetically determined by the major histocompatibility complex (MHC), refer...
journal_title:Annals of the New York Academy of Sciences
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abstract::Major trauma, shock, sepsis, and other conditions can lead to the acute respiratory distress syndrome (ARDS), which may progress to the highly lethal multiple organ dysfunction syndrome (MODS). Although a number of therapeutic strategies have been initiated, their success has been limited largely due to an incomplete ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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abstract::The presented paper describes a multi-disciplinary cooling selection approach applied to major gas turbine engine hot section components, including turbine nozzles, blades, discs, combustors and support structures, which maintain blade tip clearances. The paper demonstrates benefits of close interaction between partic...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2001.tb05854.x
更新日期:2001-05-01 00:00:00
abstract::On occasion, emerging scientific fields intersect and great discoveries result. In the last decade, the discovery of regulatory T cells (T(reg)) in immunity has revolutionized our understanding of how the immune system is controlled. Intersecting the rapidly emerging field of T(reg) function, has been the discovery th...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1443.017
更新日期:2008-11-01 00:00:00
abstract::Astrakhan fever is a summer spotted fever resembling Mediterranean spotted fever, endemic in Astrakhan, a region of Russia located by the Caspian sea. Its agent is a spotted fever group rickettsia, member of the Rickettsia conorii complex, transmitted to humans by Rhipicephalus sanguineus and Rhipicephalus pumilio tic...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
abstract::The effects of visual field changes and ocular hypertension on visual evoked potentials were investigated by photopic ERG and by luminance and pattern-reversal EPs on 116 glaucomatous and on 7 normal eyes. The problem was approached by way of four investigations: Firstly, which nerve structures are affected by glaucom...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
abstract::In a prospective investigation of perioperative cardiac edema formation requiring a delayed sternal closure, we identified thrombin increase combined with a simultaneous decrease of factor XIII as a probable cause. After experimental studies additionally revealed that factor XIII could protect endothelial barrier func...
journal_title:Annals of the New York Academy of Sciences
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2001-01-01 00:00:00
abstract::Malignancies have developed several strategies to evade immune surveillance. We have investigated pancreatic cancer cell lines and pancreatic cancer surgical specimens to evaluate possibilities of tumor escape in the Fas system, and local immune suppression. Despite Fas expression the majority of cell lines was resist...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1999.tb09529.x
更新日期:1999-06-30 00:00:00
abstract::The osteoclast (OCL) is derived from the cells in monocyte-macrophage lineage. The earliest identifiable OCL precursor is the granulocyte-macrophage colony-forming unit (CFU-GM), which gives rise to granulocytes, monocytes, and OCL. CFU-GM-derived cells then differentiate to committed OCL precursors, which are post-mi...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:2006-04-01 00:00:00
abstract::Whether T cells promote bone loss following immobilization after spinal cord injury (SCI) remains undetermined. Therefore, wild-type (WT) and T cell-deficient (Tcrb-/- ) male mice underwent sham or contusion SCI to cause hindlimb paralysis. Femurs were isolated and distal and midshaft regions were evaluated by microco...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/nyas.14518
更新日期:2020-10-26 00:00:00
abstract::This paper is dedicated to the transient liquid crystal technique measurements for multiple view access by using a novel digital recording and image processing system. The transient liquid crystal technique is widely used for heat transfer investigations in turbomachinery. It has been applied in our laboratory in seve...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2001.tb05863.x
更新日期:2001-05-01 00:00:00
abstract::Antisense oligonucleotides (AON) are being developed for a wide array of therapeutic applications. Significant improvements in their serum stability, target affinity, and safety profile have been achieved with the development of chemically modified oligonucleotides. Here, we compared 2'-deoxy-2'-fluoro-beta-D-arabinon...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:2006-10-01 00:00:00