Synthesis of alpha-fetoprotein by rat ascites hepatoma cells.

Abstract:

:AH-66 rat ascites hepatoma cells incorporated [14C]leucine into the AFP fraction. In a cell-free system, hepatoma ribosomes were found to be active in AFP synthesis whereas the supernatant fraction from hepatoma had no specific effect on AFP production. The amount of [14C]leucine incorporated in AFP by membrane-bound polysomes was 20 to 90 times higher than that by free polysomes, suggesting that AFP is mainly synthesized on membrane-bound polysomes. DBcAMP inhibited the growth of hepatoma cells. However, the incorporation of [14C]leucine into the AFP fraction as well as into total proteins was stimulated by DBcAMP.

journal_name

Ann N Y Acad Sci

authors

Kanai K,Endo Y,Oda T,Kaneko Y

doi

10.1111/j.1749-6632.1975.tb25399.x

subject

Has Abstract

pub_date

1975-08-22 00:00:00

pages

29-36

eissn

0077-8923

issn

1749-6632

journal_volume

259

pub_type

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