Effect of topoisomerase inhibitors on the in vitro HIV DNA integration reaction.

abstract：:Human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) plays a central role in the virus replication cycle. We found that HIV-1 RT was rapidly degraded when incubated with cell extracts obtained from human peripheral blood cells. The proteolytic activity responsible for the in vitro degradation of RT w...

journal_title：Biochemical and biophysical research communications

authors： Château MT,Robert-Hebmann V,Devaux C,Lazaro JB,Canard B,Coux O

更新日期：2001-07-27 00:00:00

abstract：:The current rapid progression in tissue engineering and local gene delivery system has enhanced our applications to periodontal tissue engineering. In this study, porous chitosan/collagen scaffolds were prepared through a freeze-drying process, and loaded with plasmid and adenoviral vector encoding human transforming ...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Cheng X,Wang J,Wang Y,Shi B,Huang C,Yang X,Liu T

更新日期：2006-05-26 00:00:00

abstract：:MicroRNA-125a exhibits an antiproliferative activity and is downregulated in several types of tumors, including hepatocellular carcinoma where it targets sirtuin-7, matrix metalloproteinase-11, and c-Raf. Another target of miR-125a is Lin28, a pluripotency factor that is generally undetectable in differentiated cells ...

journal_title：Biochemical and biophysical research communications

authors： Panella M,Mosca N,Di Palo A,Potenza N,Russo A

更新日期：2018-06-07 00:00:00

abstract：:The family of G-protein-activated inward-rectifiers K+ channels presently comprise at least 3 cloned members called GIRK1, GIRK2 and GIRK3. A close structural parent of GIRK channels has recently been described as being an ATP-sensitive K+ channel. This paper shows that Xenopus expression of this new channel that we c...

journal_title：Biochemical and biophysical research communications

authors： Duprat F,Lesage F,Guillemare E,Fink M,Hugnot JP,Bigay J,Lazdunski M,Romey G,Barhanin J

更新日期：1995-07-17 00:00:00

abstract：:Gaf-1/Rip11 encoded by the clone KIAA0857 participates in endosomal recycling through the interaction with both gamma-SNAP, a member of the soluble NSF attachment protein family, and a small GTPase, Rab11. Gaf-1/Rip11 and other Rab11-interacting proteins constitute a novel protein family that is involved in the endocy...

journal_title：Biochemical and biophysical research communications

authors： Kawase K,Shibata M,Kawashima H,Hatsuzawa K,Nagahama M,Tagaya M,Tani K

更新日期：2003-04-18 00:00:00

abstract：:Follistatin, activin and inhibin proteins are produced by granulosa cells, but the mechanisms controlling their production remain unclear. Here, we examined how the protein kinase A (PKA) and protein kinase C (PKC) pathways act and interact to regulate production of these proteins. Granulosa cells from immature rats w...

journal_title：Biochemical and biophysical research communications

authors： Miyanaga K,Erickson GF,DePaolo LV,Ling N,Shimasaki S

更新日期：1993-07-15 00:00:00

abstract：:Prostaglandins play a major role in the inflammatory and immune response. Macrophages constitutively produce prostaglandins by the enzyme cyclooxygenase-1 (cox) whereas inflammatory mediators and cytokines stimulate the inducible enzyme cox-2 for high output prostaglandin production. In this study, we investigated the...

journal_title：Biochemical and biophysical research communications

authors： Arias-Negrete S,Keller K,Chadee K

更新日期：1995-03-17 00:00:00

abstract：:We previously reported that moderate hypoxia and hypoxia-mimetic agents including cobalt chloride (CoCl(2)) induce differentiation of human acute myeloid leukemia (AML) cells through hypoxia-inducible factor-1 α (HIF-1 α), which interacts with and enhances transcriptional activity of CCAAT-enhancer binding factor alph...

journal_title：Biochemical and biophysical research communications

authors： Yan ZW,Hou JK,He W,Fan L,Huang Y

更新日期：2013-01-18 00:00:00

abstract：:The effect of musk xylene on contents of both cytochrome P-450IA1 and cytochrome P-450IA2 in rat liver was investigated using Western blotting analysis. Rats were treated i.p. for five consecutive days with either 50, 100 or 200 mg musk xylene/kg body weight. Musk xylene increased both total cytochrome P-450 and cytoc...

journal_title：Biochemical and biophysical research communications

authors： Iwata N,Minegishi K,Suzuki K,Ohno Y,Kawanishi T,Takahashi A

更新日期：1992-04-15 00:00:00

abstract：:The extracellular microenvironment plays a key role in regulation of cellular functions and growth control. We show here that membrane-type 1 matrix metalloproteinase (MT1-MMP) acts as a growth promoter in confluent culture. When MT1-MMP was silenced in HT1080 fibrosarcoma cells, cells created three dimensional (3D) f...

journal_title：Biochemical and biophysical research communications

authors： Takino T,Guo L,Domoto T,Sato H

更新日期：2013-07-05 00:00:00

abstract：:Protein kinase C phosphorylated both the 19/21-kDa regulatory light chains and heavy chains of bovine brain myosin. The major phosphorylation sites of the light chains were on their threonyl residues, while those for myosin light chain kinase were on their seryl residues. Whereas several non-muscle regular myosins hav...

journal_title：Biochemical and biophysical research communications

authors： Ikeda N,Yasuda S,Muguruma M,Matsumura S

更新日期：1990-06-29 00:00:00

abstract：:Extracellular superoxide dismutase (EC-SOD) is the major isozyme of SOD in arteries, but is also abundant in lungs. In particular, mouse lungs contain large amounts of EC-SOD compared to lungs in other mammals. This suggests that EC-SOD may have an amplified function in the mouse lung. This study describes the purific...

journal_title：Biochemical and biophysical research communications

authors： Fattman CL,Enghild JJ,Crapo JD,Schaefer LM,Valnickova Z,Oury TD

更新日期：2000-08-28 00:00:00

abstract：:Rat foetal hepatocytes in primary cultures were used as a model for the study of malic enzyme gene expression. Carbohydrates and glycolytic metabolites produced the precocious induction of the malic enzyme in foetal hepatocytes cultured in the absence of serum and hormones. Palmitate prevented this induction. Insulin ...

journal_title：Biochemical and biophysical research communications

authors： Fabregat I,Lorenzo M,Benito M

更新日期：1989-06-30 00:00:00

abstract：:Genomic alterations and abnormal expression of the fragile histidine triad (FHIT) gene in gastric carcinomas were examined to determine whether the FHIT gene is actually a frequent target for alteration during gastric carcinogenesis. To correlate DNA and RNA lesions of the FHIT gene with the effect on FHIT protein exp...

journal_title：Biochemical and biophysical research communications

authors： Lee SH,Kim WH,Kim HK,Woo KM,Nam HS,Kim HS,Kim JG,Cho MH

更新日期：2001-06-15 00:00:00

abstract：:Vitronectin immobilized onto polystyrene microtiter wells was demonstrated to specifically bind plasminogen in a concentration-dependent manner, yielding an estimated KD = 0.4 microM. Heparin only moderately interfered with the vitronectin-plasminogen interaction, whereas high concentrations of 6-amino-hexanoic acid i...

journal_title：Biochemical and biophysical research communications

authors： Preissner KT

更新日期：1990-05-16 00:00:00

abstract：:Recombinant scorpion anti-excitation peptide (rANEP) has previously been expressed using the pET32a system and purified via affinity chromatography. However, rANEP is expressed in BL21(DE3) cells as an inclusion body, and the affinity tag can not be removed. To overcome this problem, we used a variety of protein, DsbA...

journal_title：Biochemical and biophysical research communications

authors： Han T,Ming H,Deng L,Zhu H,Liu Z,Zhang J,Song Y

更新日期：2017-01-01 00:00:00

abstract：:Cancer cells show characteristic telomere dynamics. Their chromosomes usually have short telomeres and a high telomerase activity. The "telomere crisis model" proposed here suggests that these unique telomeric features are responsible for the progression of cancer. ...

journal_title：Biochemical and biophysical research communications

authors： Ishikawa F

更新日期：1997-01-03 00:00:00

abstract：:The subunit arrangement of wheat germ RNA polymerase II was examined using the cleavable cross-linking reagent dithiobis(succinimidyl propionate). Conditions were chosen such that the enzyme was active prior to treatment, and that most of the subunits were reactive towards the reagent. Our results indicate that the en...

journal_title：Biochemical and biophysical research communications

authors： Bateman E,Nicholson BH

更新日期：1984-06-15 00:00:00

abstract：:A sample of highly oriented Na-hyaluronate was prepared with a wet--spinning method and equilibrated to 75% relative humidity. Its proton NMR spectrum was recorded as a function of the angle theta between the hyaluronate helix axis and the external magnetic field. It appeared as a singlet with an angle-dependent line ...

journal_title：Biochemical and biophysical research communications

authors： Lahajnar G,Rupprecht A

更新日期：1986-11-26 00:00:00

abstract：:Parathyroid hormone (PTH) significantly affects osteoblast function by altering gene expression. We have identified neuron-derived orphan receptor-1 (NOR-1) as a PTH-induced primary gene in osteoblastic cells. NOR-1, Nurr1, and Nur77 comprise the NGFI-B nuclear orphan receptor family and Nurr1 and Nur77 are PTH-induce...

journal_title：Biochemical and biophysical research communications

authors： Pirih FQ,Nervina JM,Pham L,Aghaloo T,Tetradis S

更新日期：2003-06-20 00:00:00

abstract：:The activation of vitamin D requires 25-hydroxylation in the liver and 1alpha-hydroxylation in the kidney. However, it remains unclear which enzyme is relevant to vitamin D 25-hydroxylation. Recently, human CYP2R1 has been reported to be a potential candidate for a hepatic vitamin D 25-hydroxylase. Thus, vitamin D met...

journal_title：Biochemical and biophysical research communications

authors： Shinkyo R,Sakaki T,Kamakura M,Ohta M,Inouye K

更新日期：2004-11-05 00:00:00

abstract：:Treatment of platelet rich plasma (PRP) at pH 5 results in the precipitation of a protein kinase system. The protein kinase is associated with the platelet fraction and is capable of phosphorylation of several plasma proteins. Analysis of the 32P-labeled phosphoproteins by two dimensional gel electrophoresis showed th...

journal_title：Biochemical and biophysical research communications

authors： Galabru J,Krust B,Hovanessian AG

更新日期：1983-06-15 00:00:00

abstract：:The aggregation of α-synuclein is clearly related to the pathogenesis of Parkinson's disease. Therefore, detailed understanding of the mechanism of fibril formation is highly valuable for the development of clinical treatment and also of the diagnostic tools. Here, we have investigated the interaction of α-synuclein w...

journal_title：Biochemical and biophysical research communications

authors： Bae SY,Kim S,Hwang H,Kim HK,Yoon HC,Kim JH,Lee S,Kim TD

更新日期：2010-10-01 00:00:00

abstract：:COMPARE computer program suggested that benzamide riboside, BR, 3-(1-deoxy-beta-D-ribofuranosyl)benzamide, should have a similar mechanism of action as that of tiazofurin, an inhibitor of IMP dehydrogenase (IMPDH). This hypothesis was tested in K562 cells in culture. BR was cytotoxic to K562 cells with an IC50 of 2 mi...

journal_title：Biochemical and biophysical research communications

authors： Jayaram HN,Gharehbaghi K,Jayaram NH,Rieser J,Krohn K,Paull KD

更新日期：1992-08-14 00:00:00

abstract：:Acute kidney injury induced by renal ischemia-reperfusion (IR) is a prominent risk factor in the development towards renal fibrosis. Ras-related C3 botulinum toxin substrate 1(Rac1) has been involved in the pathophysiology of fibrotic disorders. But the role of Rac1 in the pathogenesis of IR-induced renal fibrosis is ...

journal_title：Biochemical and biophysical research communications

authors： Liang H,Huang J,Huang Q,Xie YC,Liu HZ,Wang HB

更新日期：2018-09-18 00:00:00

abstract：:In vitro, survival and function of rat pancreatic β-cells are optimally preserved in the presence of 10 mmol/l glucose (G10) and markedly altered by prolonged culture at either 2 mmol/l glucose (G2) or 30 mmol/l glucose (G30). The increase in islet cell apoptosis in G2 and G30 vs. G10 is preceded by parallel increases...

journal_title：Biochemical and biophysical research communications

authors： Duprez J,Jonas JC

更新日期：2011-11-18 00:00:00

abstract：:The gene encoding a red-shifted green fluorescent protein variant (EGFP) was introduced into a human immunodeficiency virus type 1 (HIV-1) molecular clone by replacing the nef gene. The EGFP-expressing HIV-1 replicated efficiently in established human T cells. The expression of EGFP in the virus-infected cells was con...

journal_title：Biochemical and biophysical research communications

authors： Lee AH,Han JM,Sung YC

更新日期：1997-04-07 00:00:00

abstract：:Glucuronidated and/or methylated metabolites of the proanthocyanidin (PA) monomer (-)-epicatechin are detected in both blood and brain following feeding of rodents with a monomeric grape seed PA extract shown to reduce symptoms in a mouse model of Alzheimer's disease. To generate metabolites for future mechanistic stu...

journal_title：Biochemical and biophysical research communications

authors： Blount JW,Ferruzzi M,Raftery D,Pasinetti GM,Dixon RA

更新日期：2012-01-06 00:00:00

abstract：:A porphyrinic sensor was used to monitor nitric oxide release from vascular smooth muscle in response to exposure to ultraviolet light. Aortic rings exposed to UV light relaxed with a time course that parallels this observed NO release. With repeated UV light treatments, the magnitude of the relaxations diminished, su...

journal_title：Biochemical and biophysical research communications

authors： Kubaszewski E,Peters A,McClain S,Bohr D,Malinski T

更新日期：1994-04-15 00:00:00

abstract：:Extra long PCR analysis of mitochondrial DNA (mtDNA) isolated from skeletal muscle of humans of different ages revealed three phenomena: (i) the amount of normal length mtDNA (16.5 kb) was progressively reduced with age, such that the cells of old age individuals (about 90 years) contained little, or undetectable amou...

journal_title：Biochemical and biophysical research communications

authors： Kovalenko SA,Kopsidas G,Kelso JM,Linnane AW

更新日期：1997-03-06 00:00:00