Leishmania infantum possesses a complex family of histone H2A genes: structural characterization and analysis of expression.

Abstract:

:We have studied the genomic organization and transcription of the histone H2A genes in the protozoan parasite Leishmania infantum. In the parasite genome 2 gene clusters exist, each containing 3 H2A gene copies. Sequence analyses showed the existence of significant sequence divergence among the H2A genes, mainly in their 5'- and 3'-untranslated regions (UTRs). Also, the existence of allelic alternatives has been evidenced. Based on the divergence in the 3'UTR regions, we have defined 3 classes of H2A transcripts, which are present at different levels in L. infantum promastigotes. However, transcription of the 3 classes of H2A genes occurs at similar levels, as measured by nuclear run-on assays, indicating that their abundance is regulated post-transcriptionally. Also, differences in regulation were observed among the H2A transcripts: the levels of transcripts with 3'-UTR type I and type III are affected by growth phase whereas transcripts with 3'-UTR type II, that are barely detected, remain constant. It is likely that the complexity, in both gene organization and differential expression exhibited by the L. infantum H2A genes, is imposed by the nature of the post-transcriptional mechanisms of regulation operating in this parasite.

journal_name

Parasitology

journal_title

Parasitology

authors

Soto M,Quijada L,Larreta R,Iborra S,Alonso C,Requena JM

doi

10.1017/s0031182003003445

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

95-105

issue

Pt 2

eissn

0031-1820

issn

1469-8161

journal_volume

127

pub_type

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