Calcification in human osteoblasts cultured in medium conditioned by the prostatic cancer cell line PC-3 and prostatic acid phosphatase.

Abstract:

:A medium that had been conditioned by PC-3 cells stimulated the calcification of a human osteoblastic cell line, Tak-10, in a nonmitogenic culture. The calcification of the osteoblasts was stimulated maximally at a 25% concentration of the conditioned medium. Calcification activity was markedly enhanced by the addition of both prostatic acid phosphatase (PAP) and its substrate, alpha-glycerophosphate, to the medium; however, PAP added alone did not enhance this activity. These results suggest that human prostatic carcinoma cells produce a factor that stimulates the calcification of the human osteoblasts. Results have also suggested that PAP is a requisite for osteogenesis provided that its substrates are abundant in the medium.

journal_name

Urol Int

journal_title

Urologia internationalis

authors

Kimura G,Sugisaki Y,Masugi Y,Nakazawa N

doi

10.1159/000282291

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

25-30

issue

1

eissn

0042-1138

issn

1423-0399

journal_volume

48

pub_type

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