Characterization of seminoma-derived placental-like alkaline phosphatase.

Abstract:

:Characteristics of placental-like alkaline phosphatase (PLAP-like enzyme) in seminoma was studied. By use of lectin affinity chromatography, PLAP-like enzyme in seminoma revealed extra sugar chains compared to placental alkaline phosphatase (PLAP), indicating heterogeneity of the carbohydrate moiety. However, the glycosylation patterns were found to be essentially similar between seminoma and normal testis. On isoelectric focusing, differences in migration patterns were revealed between seminoma-derived and normal testis-derived PLAP-like enzyme as well as between PLAP-like enzyme and PLAP. The differences in charge were mainly due to differences in sialylation of the molecules. The complex pattern of PLAP-like enzyme from seminoma on isoelectric focusing was not altered by neuraminidase treatment, indicating a considerable charge heterogeneity within the population of the enzyme molecules from the tumor.

journal_name

Urol Int

journal_title

Urologia internationalis

authors

Koshida K,Uchibayashi T,Hisazumi H

doi

10.1159/000282263

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

96-9

eissn

0042-1138

issn

1423-0399

journal_volume

47 Suppl 1

pub_type

杂志文章
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