Abstract：
:Radioactivity from I125-labeled human platelets was measured to estimate the extent of binding of platelet surface proteins to immobilized thrombin. 1-3% of the radioactivity was bound with 10-20% of this amount apparently irreversibly bound to the thrombin matrix. Site-specific chemical modification of thrombin with pyridoxal-5'-phosphate, N-bromosuccinimide or tetranitromethane resulted in a variable reduction of the amount of radiolabel bound. When thrombin modified with H-D-PheProArg-chloromethyl ketone (PPACK) was coupled to the matrix, there was no difference in the binding of platelet membrane proteins when compared to a control thrombin preparation while thrombin modified with tosyl-Lys-chloromethyl ketone (TLCK) coupled to the matrix did not bind radiolabel any more effectively than albumin which served as the control. However, when thrombin was modified with PPACK after coupling to the agarose matrix, ability to bind radiolabel was lost. Thrombin bound to platelets remained catalytically active when assayed with a peptide nitroanilide substrate. These results suggest tight binding between thrombin and platelets that is not only not dependent on active site integrity but leaves the bound thrombin catalytically competent.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Wu HF,White GC 2nd,Workman EF Jr,Jenzano JW,Lundblad RLdoi
10.1016/0049-3848(92)90271-bsubject
Has Abstractpub_date
1992-08-15 00:00:00pages
419-27issue
4eissn
0049-3848issn
1879-2472journal_volume
67pub_type
杂志文章abstract：:Hip joint replacement surgery, using acrylic cement for prosthesis fixation, is associated with intraoperative cardiorespiratory dysfunction, and a high frequency of postoperative proximal deep vein thrombosis (DVT). Levels of prothrombin fragments 1+2 (F1+2), tissue plasminogen activator antigen (t-PA), plasminogen a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90087-5
更新日期：1993-06-15 00:00:00
abstract：BACKGROUND:There is a perception in the orthopaedic and thromboembolism community that the incidence of deep vein thrombosis (DVT) has decreased in patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA). OBJECTIVES:To assess the incidence of DVT with warfarin thromboprophylaxis over time in ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2008.05.005
更新日期：2008-01-01 00:00:00
abstract：:Protein C has been measured by three different assays (antigenic, amidolytic and chronometric) in 27 end-stage renal insufficient patients before and after hemodialysis. Protein C levels have been compared with other coagulation inhibitors (antithrombin III, protein S) and fibrinolytic parameters. Baseline anticoagula...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90055-s
更新日期：1993-03-15 00:00:00
abstract：:Trimethylamine-N-oxide (TMAO), one of the products in choline metabolite, is recently reported to be associated with cardiovascular diseases (CVD) that mainly attribute to atherothrombosis. However, the mechanisms how TMAO functions in the pathogenesis of CVD and atherothrombosis remain elusive. Tissue factor (TF) has...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.02.028
更新日期：2019-05-01 00:00:00
abstract：:Disseminated intravascular coagulation (DIC) is a severe complication of septic shock. Polymorphonuclear neutrophils (PMNs) may play a key role in septic shock-induced DIC via the release of neutrophils extracellular traps (NETs). NETs capture invading pathogens, but also act as a pro-coagulant surface at the interfac...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2019.09.036
更新日期：2019-11-01 00:00:00
abstract：:A blinded randomized multicenter trial of two non-activated prothrombin complex concentrates was carried out to determine the clinical effectivity in the treatment of acute hemarthrosis in hemophiliac patients with inhibitors. The one product was prepared via DEAE Sephadex chromatography, while the second was fraction...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0049-3848(86)90115-5
更新日期：1986-06-15 00:00:00
abstract：:In the treatment of unstable coronary artery disease, direct thrombin inhibitors have shown no or only limited benefit as compared with heparin, despite theoretical advantages. One explanation may be that the direct thrombin inhibitors to a greater extent than heparin have an inhibiting effect on the generation and ac...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00029-8
更新日期：1999-07-15 00:00:00
abstract：BACKGROUND:Ultrasound has the potential to augment chemical thrombolysis. METHODS AND RESULTS:Thrombotic occlusions in the left circumflex artery (LCx) were induced in 27 dogs. Sixty minutes later, tissue-type plasminogen activator (t-PA) was given intravenously over 90 min. Thrombotic occlusions (n = 20) were treated...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(03)00335-9
更新日期：2003-05-01 00:00:00
abstract：:While the role of platelets in hemostasis is well characterized from a biological perspective, the biophysical interactions between platelets and their mechanical microenvironment are relatively unstudied. The field of cellular mechanics has developed a number of approaches to study the effects of extracellular matrix...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2013.12.037
更新日期：2014-04-01 00:00:00
abstract：:Anticoagulants used for platelet function studies in vitro may affect platelet responsiveness. In the present study we compared the influence of three different anticoagulants, sodium citrate, low molecular mass heparin, and recombinant hirudin, on platelet aggregation in whole blood in vitro, using impedance aggregom...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00114-x
更新日期：1997-07-01 00:00:00
abstract：INTRODUCTION:The activated partial thromboplastin time (APTT) is commonly used to monitor unfractionated heparin (UFH) but may not accurately measure the amount of heparin present. The anti-Xa assay is less susceptible to confounding factors and may be a better assay for this purpose. MATERIALS AND METHODS:The validit...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.07.006
更新日期：2017-09-01 00:00:00
abstract：:In spite of numerous studies on the involvement of fibrinogen in transendothelial migration of leukocytes and thereby inflammation, there is still no clear understanding of which fibrin(ogen) species can stimulate leukocyte transmigration. Although we have previously proposed that interaction of fibrin with the VLDL r...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.11.007
更新日期：2018-02-01 00:00:00
abstract：PURPOSE:The right ventricular outflow tract (RVOT) velocity time integral (VTI), an echocardiographic measure of stroke distance, correlates with cardiac index. We sought to determine the prognostic significance of low RVOT VTI on clinical outcomes among patients with acute pulmonary embolism (PE). MATERIALS AND METHO...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.06.049
更新日期：2020-11-01 00:00:00
abstract：:The effects of sodium salicylate (SS) on ADP-induced platelet aggregation and on a metabolism of calcium in platelets were studied, using gel-filtrated platelets (GFP). SS inhibited dose-responsively ADP-induced aggregation in the presence of fibrinogen and Ca2+. It was found that extracellular 45Ca2+ was rapidly take...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90232-4
更新日期：1985-08-01 00:00:00
abstract：:When human citrated plasma is dialysed against a phosphate buffer containing Ca++, citrate anions are removed, thrombin is generated and soluble fibrinogen derivatives (fibrin monomers and/or soluble fibrin polymers) are formed. These derivatives are able to combine with human or bovine elastin to form a very stable a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90061-7
更新日期：1986-07-15 00:00:00
abstract：INTRODUCTION:Monocyte- and microparticle (MP)-associated tissue factor (TF) is upregulated in diabetes. Lipopolysaccharide (LPS) induces expression of TF and alternatively spliced TF (asTF) and increases MP release from monocytes. Using LPS-stimulated TF-bearing human monocytes, we examined whether glibenclamide, a sul...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.07.007
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:Influencing factors for clopidogrel-mediated platelet inhibition have only been evaluated by one or two different test systems in the same population so far. Since previous studies revealed poor correlations between the various platelet function tests, the identification of influencing variables for clopidog...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2011.05.008
更新日期：2011-10-01 00:00:00
abstract：:In this paper, we demonstrated that the procoagulant action of Lonomia achelous (Cramer) is due in part to a component that activates prothrombin. The activation by crude venom and Fractions obtained by gel filtration on Sephadex G-75 is not dependent of phospholipid, Ca++ or Factor V. The activation of prothrombin by...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90187-f
更新日期：1992-05-01 00:00:00
abstract：:Anticoagulant fucoidan fractions of different molecular weight and sulfate content were prepared and investigated for their effects on platelet function in vitro. The fucoidan fractions were incubated with human platelet rich plasma (PRP) at concentrations of 5, 10 and 50 micrograms/ml. Platelet activation was subsequ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00037-6
更新日期：1997-03-15 00:00:00
abstract：:The extracellular nucleotides, ATP and ADP, as well as adenosine have been implicated in a great number of physiological functions. ADP is one of the major platelet recruiting factors, whereas ATP is considered to be a competitive inhibitor of ADP-induced platelet aggregation and adenosine is able to induce vasodilata...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.01.006
更新日期：2007-01-01 00:00:00
abstract：INTRODUCTION:Management of pregnant women at risk for venous thromboembolism (VTE) remains complex. Guidelines do not definitively fix optimal strategies due to limited trial data. Our objective was to build an easy-to-use tool allowing individualised, risk-adapted prophylaxis. MATERIALS AND METHODS:A Delphi exercise ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.01.012
更新日期：2010-08-01 00:00:00
abstract：BACKGROUND:It has been reported that statin therapy produces additional effects including impaired activation of blood coagulation. It is not clear whether statins can affect hemostasis in patients with acute coronary syndrome. The aim of this study was to investigate the effect of prior statin treatment on thrombin ge...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2009.06.021
更新日期：2010-05-01 00:00:00
abstract：IMPORTANCE:Unprovoked venous thromboembolism (VTE) can be the first manifestation of cancer. It is unclear if extensive screening for occult cancer including a comprehensive computed tomography (CT) scan of the abdomen/pelvis is cost-effective in this patient population. OBJECTIVE:To assess the health care related cos...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2017.01.007
更新日期：2017-03-01 00:00:00
abstract：:PAF (1 ug/kg) injected intravenously (i.v.) into anesthetized rabbits resulted in marked loss of circulating platelets and leukocytes. Administration of FR-900452 1-methyl-3-(1-(5-methylthiomethyl-6-oxo-3-(2-oxo-3-cyclopenten-1-y lidene)- 2-piperazinyl) ethyl)-2-indolinone, a specific PAF inhibitor, at a dose of 10 mg...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)90344-0
更新日期：1986-06-01 00:00:00
abstract：BACKGROUND:Although patients with idiopathic VTE are at higher than normal risk of asymptomatic atherosclerosis and of cardiovascular events, the impact of cardiovascular risk factors on VTE is poorly understood. OBJECTIVE:To assess the prevalence of the metabolic syndrome and of its components in patients with early-...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2010.12.005
更新日期：2011-03-01 00:00:00
abstract：:Concentration-response relationships of human platelet aggregation rates were analyzed for a variety of agonists and inhibitors. Their approximation by the Hill equation showed that the values of the Hill coefficient (h) were agonist-dependent and increased as follows: hADP = hL-EPINEPHRINE = hPAF = hPGH2 = hU46619 le...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90308-w
更新日期：1992-06-01 00:00:00
abstract：:The Coa-set Fibrin Monomer test (CFM-test) is a quantitative method for determination of soluble fibrin in plasma. The fibrin standard of the CFM-test is produced by bathroxobin conversion of purified fibrinogen to fibrin. Plasma treated with minute amounts of thrombin may be considered as a more physiological way of ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90139-f
更新日期：1993-11-15 00:00:00
abstract：:The effect of the combined oral administration of pentoxifylline (pof) and low dose acetylsalicylic acid (ASA) was evaluated with the help of the laser-induced thrombosis in rat mesenteric arterioles. Laser-induced thrombosis is inhibited in a dose-dependent way by both drugs. The administration of ASA, either simulta...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90299-4
更新日期：1987-04-15 00:00:00
abstract：:Use of low molecular weight heparins (LMWHs) is now generally accepted as an ideal method of prophylaxis against postoperative venous thromboembolism. However, the long-term effects on the development of postthrombotic deep vein insufficiency are not known. Every patient developing such complications consumes a vast a...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/0049-3848(95)00232-4
更新日期：1996-01-01 00:00:00
abstract：INTRODUCTION:Warfarin is the most widely prescribed vitamin K antagonist and in the United States and Europe more than 10 million people are currently in long-term oral anticoagulant treatment. This study aims to retrospectively validate a dynamic statistical model providing dosage suggestions to patients in warfarin t...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.12.042
更新日期：2014-03-01 00:00:00
