Modulation of bradykinin-induced calcium signals by oxidative stress in PC12 cells.

abstract：:Recombinant glutathione S-transferase P (GST-P) was purified in a homogeneous state. Fatty acid analysis of the enzyme revealed that the final enzyme preparation endogenously bound fatty acids, mostly palmitic acid or stearic acid, which were difficult to dissociate from the complex. Temperature-dependent analysis by ...

journal_title：Archives of biochemistry and biophysics

authors： Nishihira J,Ishibashi T,Sakai M,Tsuda S,Hikichi K

更新日期：1993-04-01 00:00:00

abstract：:The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important in the detoxification of a number of endogenous and exogenous compounds, including 5-hydroxytryptamine and morphine. Studies were designed to investigate the extrahepatic expression of human UGTs using RT-PCR techniques and to determine th...

journal_title：Archives of biochemistry and biophysics

authors： King CD,Rios GR,Assouline JA,Tephly TR

更新日期：1999-05-01 00:00:00

abstract：:Flavopiridol has been shown to induce cell cycle arrest and apoptosis in various tumor cells in vitro and in vivo. Using immobilized flavopiridol, we identified glycogen phosphorylases (GP) from liver and brain as flavopiridol binding proteins from HeLa cell extract. Purified rabbit muscle GP also bound to the flavopi...

journal_title：Archives of biochemistry and biophysics

authors： Kaiser A,Nishi K,Gorin FA,Walsh DA,Bradbury EM,Schnier JB

更新日期：2001-02-15 00:00:00

abstract：:A10 smooth muscle cells, derived from embryonic rat thoracic aorta, responded to the atrial natriuretic factor (ANF) with increased levels of cyclic GMP. These cells possess high-affinity (apparent Kd = 50 pM) plasma membrane receptors for ANF. Internalization of ANF at 37 degrees C was indicated by the following: app...

journal_title：Archives of biochemistry and biophysics

authors： Napier MA,Arcuri KE,Vandlen RL

更新日期：1986-08-01 00:00:00

abstract：:Laser Raman spectroscopy has been applied to native and dithiothreitol-treated bovine cortical gamma II crystallin to examine the state of the thiol groups and the presence of a putative disulfide bridge. The data reveal significant differences in two key spectral regions. In the thiol stretching region (2500-2600 cm-...

journal_title：Archives of biochemistry and biophysics

authors： Pande J,McDermott MJ,Callender RH,Spector A

更新日期：1989-02-15 00:00:00

abstract：:This study showcases the potential of unnatural amino acids to enable non-natural functions when incorporated in the protein scaffold of heme metalloproteins. For this purpose, a genetically-engineered myoglobin (Mb) mutant was created by incorporating redox-active 3-amino-l-tyrosine (NH2Tyr) into its active site, rep...

journal_title：Archives of biochemistry and biophysics

authors： Chand S,Ray S,Wanigasekara E,Yadav P,Crawford JA,Armstrong DW,Rajeshwar K,Pierce BS

更新日期：2018-02-01 00:00:00

abstract：:MicroRNA-885 (miR-885) has been shown to act as vital regulator of tumorigenesis and its tumor-suppressive role has been investigated in several human cancers. However, the role of miR-885 in regulation of epithelial mesenchymal transition of liver cancer cells yet unknown. This study was undertaken to investigate the...

journal_title：Archives of biochemistry and biophysics

authors： Feng L,Zhang Y,Yang Q,Guo L,Yang F

更新日期：2020-10-30 00:00:00

abstract：:The intracellular concentration of many steroids and xenobiotics is influenced by the membrane protein P-glycoprotein (Pgp). It has been inferred that the intracellular retention of many drugs that upregulate Pgp or modulate Pgp function might also be affected by Pgp. However, the ability of Pgp to influence the trans...

journal_title：Archives of biochemistry and biophysics

authors： Schuetz EG,Yasuda K,Arimori K,Schuetz JD

更新日期：1998-02-15 00:00:00

abstract：:A cDNA from Arabidopsis thaliana resembling S-formylglutathione hydrolase (SFGH), an enzyme with putative roles in formaldehyde detoxification in animals and microorganisms, has been cloned and expressed in Escherichia coli. The purified recombinant Arabidopsis enzyme (AtSFGH) was a dimer composed of 31-kDa subunits. ...

journal_title：Archives of biochemistry and biophysics

authors： Kordic S,Cummins I,Edwards R

更新日期：2002-03-15 00:00:00

abstract：:This study characterizes the structural and functional significance of sulfhydryl residues in human plasma heparin cofactor II (HCII). For quantification of sulfhydryl groups, the extinction coefficient of HCII was redetermined and found to be 0.593 ml mg-1 cm-1 using second-derivative spectroscopy and multicomponent ...

journal_title：Archives of biochemistry and biophysics

authors： Church FC,Meade JB,Pratt CW

更新日期：1987-12-01 00:00:00

abstract：:The phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene. These carotenes are intermediates for the synthesis of spheroidene and spirilloxanthin, respectively. Two different mutation libraries for the crtI gene from R...

journal_title：Archives of biochemistry and biophysics

authors： Stickforth P,Sandmann G

更新日期：2011-01-01 00:00:00

abstract：:According to differences in mobility on SDS-polyacrylamide gel electrophoresis, calpastatins (inhibitor proteins of the calcium-dependent proteinase calpain) are classified into the tissue type (100-120 kDa) and the erythrocyte type (70 kDa), which lacks the amino-terminal domains (domains L and 1). We investigated th...

journal_title：Archives of biochemistry and biophysics

authors： Takano E,Nosaka T,Lee WJ,Nakamura K,Takahashi T,Funaki M,Okada H,Hatanaka M,Maki M

更新日期：1993-06-01 00:00:00

abstract：:Nitration of protein tyrosine residues to 3-nitrotyrosine (NO2Tyr) serves as both a marker and mediator of pathogenic reactions of nitric oxide (*NO), with peroxynitrite (ONOO-) and leukocyte peroxidase-derived nitrogen dioxide (*NO2) being proximal mediators of nitration reactions in vivo. Cytochrome c is a respirato...

journal_title：Archives of biochemistry and biophysics

authors： Castro L,Eiserich JP,Sweeney S,Radi R,Freeman BA

更新日期：2004-01-01 00:00:00

abstract：:FtsZ (Filamentous temperature sensitivity Z) cell division protein from Escherichia coli binds the fluorescence probe DAPI. Bundling of FtsZ was facilitated in the presence of DAPI, and the polymers in solution remained polymerized longer time than the protofilaments formed in the absence of DAPI. DAPI decreased both ...

journal_title：Archives of biochemistry and biophysics

authors： Nova E,Montecinos F,Brunet JE,Lagos R,Monasterio O

更新日期：2007-09-15 00:00:00

abstract：:Green tea (Camellia sinensis) is rich in catechins, of which (-)-epigallocatechin-3-gallate (EGCG) is the most abundant. Studies in animal models of carcinogenesis have shown that green tea and EGCG can inhibit tumorigenesis during the initiation, promotion and progression stages. Many potential mechanisms have been p...

journal_title：Archives of biochemistry and biophysics

authors： Lambert JD,Elias RJ

更新日期：2010-09-01 00:00:00

abstract：:1-O-Sinapoylglucose:choline sinapoyltransferase ("sinapine synthase") has been purified from immature seeds of Brassica napus by sequential hydroxylapatite absorption, ion-exchange chromatography, and gel filtration. The purified enzyme has an apparent molecular weight of 65 kDa on gel filtration and a subunit structu...

journal_title：Archives of biochemistry and biophysics

authors： Vogt T,Aebershold R,Ellis B

更新日期：1993-02-01 00:00:00

abstract：:Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

journal_title：Archives of biochemistry and biophysics

authors： Banerjee SK,Mudd JB

更新日期：1992-05-15 00:00:00

abstract：:1-O-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) induced phosphorylation of two proteins having molecular masses of approximately 20- and 40-kDa in washed rabbit platelets in a concentration- and time-dependent manner. Sequential stimulation with AGEPC did not induce additional protein phosphorylation, supportin...

journal_title：Archives of biochemistry and biophysics

authors： Sugatani J,Hanahan DJ

更新日期：1986-05-01 00:00:00

abstract：:Glycogen synthase was isolated from rat H4IIE hepatoma cells by the use of specific antibodies. Immunoprecipitates from cells grown in the presence of [35S]methionine contained two 35S-labeled polypeptides, designated GS1 and GS2, separable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Labeling of both...

journal_title：Archives of biochemistry and biophysics

authors： Armstrong DJ,Roach PJ

更新日期：1989-11-15 00:00:00

abstract：:Considerable metabolic energy is expended in ensuring that membranes possess a characteristic fatty acid composition. The nature of the specific requirement of the retina for high levels of docosahexaenoic acid (DHA) is as yet undefined. Previous work has speculated that DHA is required to maintain the fluid nature an...

journal_title：Archives of biochemistry and biophysics

authors： Treen M,Uauy RD,Jameson DM,Thomas VL,Hoffman DR

更新日期：1992-05-01 00:00:00

abstract：:Intensively treating type I diabetics with continuous subcutaneous insulin infusions or multiple daily insulin injections to normalize mean blood glucose concentrations significantly reduces the onset of secondary diabetic complications when compared to conventionally treated diabetics. Our studies focused on characte...

journal_title：Archives of biochemistry and biophysics

authors： Kramer KL,Giffin BF,Fox JW,Drake RL

更新日期：1999-08-15 00:00:00

abstract：:This paper presents previously unobserved signals in the 1H NMR spectra of oxidized and reduced [2Fe-2S]-ferredoxin from Anabaena 7120 detected at 400, 500, and 600 MHz. The signals shifted to low field exhibited longitudinal relaxation (T1) values in the range of 100-400 microseconds and line widths in the range of 1...

journal_title：Archives of biochemistry and biophysics

authors： Skjeldal L,Westler WM,Markley JL

更新日期：1990-05-01 00:00:00

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:...

journal_title：Archives of biochemistry and biophysics

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-01 00:00:00

abstract：:We previously showed that knockdown of the anaplerotic enzyme pyruvate carboxylase in the INS-1 832/13 insulinoma cell line inhibited glucose-stimulated insulin release and glucose carbon incorporation into lipids. We now show that knockdown of fatty acid synthase (FAS) mRNA and protein also inhibits glucose-stimulate...

journal_title：Archives of biochemistry and biophysics

authors： MacDonald MJ,Hasan NM,Dobrzyn A,Stoker SW,Ntambi JM,Liu X,Sampath H

更新日期：2013-04-01 00:00:00

abstract：:A chromatographic adsorbent (TED-Sepharose) capable of chelating various di- and trivalent metal ions was prepared by introducing tris(carboxymethyl)ethylenediamine into agarose. This gel was charged with Al3+, Ga3+, In3+, and Tl3+ to form immobilized-metal affinity adsorbents, and their adsorption behavior toward ser...

journal_title：Archives of biochemistry and biophysics

authors： Porath J,Olin B,Granstrand B

更新日期：1983-09-01 00:00:00

abstract：:The reaction by which the antioxidant capacity of alpha-tocopherol is sustained by recycling of the alpha-tocopheroxyl radical in membranes or lipoproteins by aqueous ascorbate has been studied by laser flash photolysis in model micellar and membrane systems. In bilayers of dimyristoylphosphatidylcholine at 35 degrees...

journal_title：Archives of biochemistry and biophysics

authors： Bisby RH,Parker AW

更新日期：1995-02-20 00:00:00

abstract：:Cutaneous malignant melanomas originate primarily within epidermal melanocytic cells. Melanoma cells share many characteristics with melanocyte precursors, suggesting that melanoma cells utilize the developmental programs of their normal counterpart for their own progression. The pigmentation system provides an advant...

journal_title：Archives of biochemistry and biophysics

authors： Liu J,Fukunaga-Kalabis M,Li L,Herlyn M

更新日期：2014-12-01 00:00:00

abstract：:The zinc-binding motif (HELLGH) of dipeptidyl peptidase III (DPP III) is different from the common zinc-binding motif (HExxH) of metallopeptidases. To clarify the importance of the zinc-binding motif part of DPP III for enzymatic activity, we measured the recovery of the enzyme activity of apo-Leu(453)-deleted dipepti...

journal_title：Archives of biochemistry and biophysics

authors： Hirose J,Kamigakiuchi H,Iwamoto H,Fujii H,Nakai M,Takenaka M,Kataoka R,Sugahara M,Yamamoto S,Fukasawa KM

更新日期：2004-11-01 00:00:00

abstract：:The fugu (pufferfish) genome has been sequenced, and a second genome assembly was released 17 May 2002. Exhaustive searches were made to identify all P450 genes and pseudogenes from the earlier release of 26 October 2001. P450 genes assembled as completely as possible from these data were used to do additional searche...

journal_title：Archives of biochemistry and biophysics

authors： Nelson DR

更新日期：2003-01-01 00:00:00

abstract：:The effect of transition from a respiring to a respiration-inhibited state on the rate of protein synthesis was investigated in glycolyzing, cultured rat heart cells. The rate was found to be significantly lower after blocking respiration, and it was further decreased by L-lactate. In contrast, pyruvate or phenazine m...

journal_title：Archives of biochemistry and biophysics

authors： Ravid K,Diamant P,Avi-Dor Y

更新日期：1985-01-01 00:00:00