当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > 4',6-Diamidino-2-phenylindole (DAPI) induces bundling of Escherichia coli FtsZ polymers inhibiting the GTPase activity.

4',6-Diamidino-2-phenylindole (DAPI) induces bundling of Escherichia coli FtsZ polymers inhibiting the GTPase activity.

Abstract：

:FtsZ (Filamentous temperature sensitivity Z) cell division protein from Escherichia coli binds the fluorescence probe DAPI. Bundling of FtsZ was facilitated in the presence of DAPI, and the polymers in solution remained polymerized longer time than the protofilaments formed in the absence of DAPI. DAPI decreased both the maximal velocity of the GTPase activity and the Michaelis-Menten constant for GTP, indicating that behaves like an uncompetitive inhibitor of the GTPase activity favoring the GTP form of FtsZ in the polymers. The results presented in this work support a cooperative polymerization mechanism in which the binding of DAPI favors protofilament lateral interactions and the stability of the resulting polymers.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Nova E,Montecinos F,Brunet JE,Lagos R,Monasterio O

doi

10.1016/j.abb.2007.06.032

subject

Has Abstract

pub_date

2007-09-15 00:00:00

pages

315-9

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(07)00343-8

journal_volume

465

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Losartan, an antagonist of AT1 receptor for angiotensin II, attenuates lipopolysaccharide-induced acute lung injury in rat.

    abstract：:Angiotensin II (Ang II) plays an important role in inflammatory process. Acute lung injury (ALI), an inflammatory disorder of the lung, is commonly associated with endotoxemia; however, the mechanism that endotoxin (lipopolysaccharide, LPS) induces the inflammatory response in ALI is not well defined. Here, we showed,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.09.019

    authors： Liu L,Qiu HB,Yang Y,Wang L,Ding HM,Li HP

    更新日期：2009-01-01 00:00:00

  • Purification and properties of a mitochondrial NAD+ glycohydrolase.

    abstract：:A 60- to 70-fold purification of an NAD+ glycohydrolase from the inner membrane of rat liver mitochondria to apparent homogeneity on sodium dodecyl sulfate (SDS)-polyacrylamide slab gel is described. The minimum molecular weight of the enzyme on polyacrylamide gels in the presence of SDS is around 62,000. The enzyme s...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90220-5

    authors： Moser B,Winterhalter KH,Richter C

    更新日期：1983-07-01 00:00:00

  • Regulatory aspects of the glutamylation of methotrexate in cultured hepatoma cells.

    abstract：:The glutamylation of methotrexate has been evaluated in H35 hepatoma cells in vitro as a function of the conditions of culture. Glutamylation yields methotrexate polyglutamate with two to five additional glutamate residues and is a saturable process. The rate of glutamylation increases little above 10 microM extracell...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90337-5

    authors： Nimec Z,Galivan J

    更新日期：1983-10-15 00:00:00

  • Modulation of fibroblast motility by a cytosolic extract of Cyanobacteria.

    abstract：:Proliferation and migratory behavior of L929 murine fibroblasts were shown to be modified in the presence of a cytosolic extract of Phormidium sp. (Cyanobacteria). The addition of Phormidium extract to the growth medium (Dulbecco's modified Eagle's medium) supplemented with 0.5% newborn calf serum increased cell proli...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90011-7

    authors： Hodara ML,Bré MH,Bouzinba H,Pouphile M,Lefort-Tran M

    更新日期：1991-04-01 00:00:00

  • The effects of iron overload on mitochondrial function, mitochondrial dynamics, and ferroptosis in cardiomyocytes.

    abstract：:Excessive iron accumulation in the heart can lead to iron overload cardiomyopathy (IOC), the leading cause of death in hemochromatosis patients. Current understanding regarding the mechanism by which iron overload causes a deterioration in cardiac performance, mitochondrial dysfunction, and impaired mitochondrial dyna...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2019.108241

    authors： Sumneang N,Siri-Angkul N,Kumfu S,Chattipakorn SC,Chattipakorn N

    更新日期：2020-02-15 00:00:00

  • Nuclear Ah receptor from mouse hepatoma cells: effect of partial proteolysis on relative molecular mass and DNA-binding properties.

    abstract：:The nuclear Ah receptor from mouse hepatoma (Hepa-1c1c9) cells is a 176-kDa multimeric protein which is stable under conditions of up to 1 M KCl. Under denaturing conditions, the Hepa-1 nuclear receptor can be dissociated into a ligand-binding subunit of Mr approximately 91,000. The identity of subunits that compose t...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(90)90670-t

    authors： Prokipcak RD,Denison MS,Okey AB

    更新日期：1990-12-01 00:00:00

  • Purification and characterization of cytochrome P450 2E2 from hepatic microsomes of neonatal rabbits.

    abstract：:The alcohol-inducible P450 2E subfamily in the rabbit has two known members that differ in only 16 amino acid residues scattered throughout the polypeptide chain. P450 2E1 has been thoroughly characterized, and is known to have diverse inducers and substrates. Little is known, however, about the properties of P450 2E2...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90134-5

    authors： Ding XX,Pernecky SJ,Coon MJ

    更新日期：1991-12-01 00:00:00

  • A novel hepatopancreatic phospholipase A2 from Hexaplex trunculus with digestive and toxic activities.

    abstract：:A marine snail digestive phospholipase A2 (mSDPL) was purified from delipidated hepatopancreas. Unlike known digestive phospholipases A2, which are 14 kDa proteins, the purified mSDPL has a molecular mass of about 30 kDa. It has a specific activity of about 180 U/mg measured at 50 degrees C and pH 8.5 using phosphatid...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2009.11.020

    authors： Zarai Z,Bacha AB,Horchani H,Bezzine S,Zouari N,Gargouri Y,Mejdoub H

    更新日期：2010-02-15 00:00:00

  • The hidden face of food phenolic composition.

    abstract：:Plant polyphenols are extremely diverse, due to the occurrence of several basic structures, numerous substitutions and, for some groups, of polymers (tannins). Plant polyphenol composition depends on the plant species and organ, with some molecules specific of particular plant families while others are ubiquitous. The...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2010.03.018

    authors： Tarascou I,Souquet JM,Mazauric JP,Carrillo S,Coq S,Canon F,Fulcrand H,Cheynier V

    更新日期：2010-09-01 00:00:00

  • Thiols in oxidative phosphorylation: thiols in the F0 of ATP synthase essential for ATPase activity.

    abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90085-3

    authors： Yagi T,Hatefi Y

    更新日期：1987-04-01 00:00:00

  • A mechanism for phorbol ester-mediated regulation of the PAF receptor in human neutrophils.

    abstract：:Phorbol esters induce rapid downregulation of PAF receptors on human neutrophils. The molecular mechanism responsible for this observation has not been elucidated. In the present study, treatment of neutrophils with PMA (10(-7) M) for 10 min caused a complete loss of PAF receptors from the cell surface as determined b...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1375

    authors： Zhou W,Olson MS

    更新日期：1994-08-15 00:00:00

  • The molecular weight and oligomerization of rabbit thrombomodulin as assessed by sedimentation equilibrium.

    abstract：:Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90115-x

    authors： Winnard PT,Esmon CT,Laue TM

    更新日期：1989-02-15 00:00:00

  • Regulatory aspects of rat liver mitochondrial phospholipase A2: effects of calcium ions and calmodulin.

    abstract：:A comparative study was made of the metal ion requirement of rat liver mitochondrial phospholipase A2 in purified and membrane-associated forms. Membrane-bound enzyme was assayed using either exogenous or endogenous phosphatidylethanolamine. Although several divalent metal ions caused increased activity of the membran...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90346-1

    authors： De Winter JM,Korpancova J,van den Bosch H

    更新日期：1984-10-01 00:00:00

  • New analogs of 2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 with conformationally restricted side chains: evaluation of biological activity and structural determination of VDR-bound conformations.

    abstract：:We have successfully prepared E- and Z- isomers of 17-20 dehydro analogs of 2-methylene-19-nor-(20S)-1alpha,25-dihydroxyvitamin D3 (2MD). Both isomers bind to the recombinant rat vitamin D receptor (VDR) with high affinity. The Z-isomer (Vit-III 17-20Z) displays activity in vivo and in vitro that is similar to 2MD. Th...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2006.11.029

    authors： Vanhooke JL,Tadi BP,Benning MM,Plum LA,DeLuca HF

    更新日期：2007-04-15 00:00:00

  • Bacterial expression of functional membrane-bound thromboxane synthase having intact sequence and truncated N-terminal hydrophobic segment.

    abstract：:A full-length cDNA for human placental thromboxane synthase and a shortened cDNA lacking the sequence corresponding to the N-terminal 2-29 amino acids were expressed in Escherichia coli using a pCW expression vector. Both intact and truncated recombinant enzyme were found in the membrane fraction and were catalyticall...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1427

    authors： Xia Z,Tai HH

    更新日期：1995-08-20 00:00:00

  • Binding and stabilization of transthyretin by curcumin.

    abstract：:Biophysical evidences suggest that transthyretin (TTR) tetramer dissociation to the monomeric intermediate and subsequent polymerization leads to amyloid fibril formation, which is implicated in the pathogenesis of familial amyloid polyneuropathy (FAP) and senile systemic amyloidosis (SSA). Hence, inhibition of fibril...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2009.02.013

    authors： Pullakhandam R,Srinivas PN,Nair MK,Reddy GB

    更新日期：2009-05-15 00:00:00

  • Modification of an essential amino group of phosphoenolpyruvate carboxylase from maize leaves by pyridoxal phosphate and by pyridoxal phosphate-sensitized photooxidation.

    abstract：:Phosphoenolpyruvate carboxylase from maize leaves was inactivated by pyridoxal 5'-phosphate in the dark and in the light. A two-step reversible mechanism is proposed for inactivation in the dark, which involves the formation of a noncovalent complex prior to a Schiff base with amino groups of the enzyme. Spectral anal...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90309-7

    authors： Podesta FE,Iglesias AA,Andreo CS

    更新日期：1986-05-01 00:00:00

  • Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex.

    abstract：:Coenzyme Q (Q) is a redox active lipid that is an essential component of the electron transport chain. Here, we show that steady state levels of Coq3, Coq4, Coq6, Coq7 and Coq9 polypeptides in yeast mitochondria are dependent on the expression of each of the other COQ genes. Submitochondrial localization studies indic...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.02.016

    authors： Hsieh EJ,Gin P,Gulmezian M,Tran UC,Saiki R,Marbois BN,Clarke CF

    更新日期：2007-07-01 00:00:00

  • Gene structure and expression of the targeting subunit, RGL, of the muscle-specific glycogen-associated type 1 protein phosphatase, PP1G.

    abstract：:The type I phosphatase associated with glycogen, PP1G, plays an important role in glycogen metabolism. PP1G is targeted to glycogen by the R(GL) subunit, which regulates the function of the enzyme. We report the cloning and characterization of the gene as well as the pattern of expression of the R(GL) subunit from mou...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2001.2283

    authors： Lannér C,Suzuki Y,Bi C,Zhang H,Cooper LD,Bowker-Kinley MM,DePaoli-Roach AA

    更新日期：2001-04-01 00:00:00

  • Regulation of the Ca2+-dependent neutral proteinases from rabbit liver by an endogenous inhibitor.

    abstract：:An endogenous inhibitor of neutral Ca2+-dependent proteinases has been isolated from rabbit liver cytosol. The inhibitor is a heat-stable, 240-kDa, tetrameric protein. It is dissociated into its 60-kDa subunits by high concentrations of Ca2+ (0.1-1 mM), but not by lower concentrations in the physiological range. Inhib...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90568-x

    authors： Melloni E,Salamino F,Sparatore B,Michetti M,Pontremoli S,Horecker BL

    更新日期：1984-08-01 00:00:00

  • Purification and properties of versiconal cyclase from Aspergillus parasiticus.

    abstract：:Versiconal cyclase catalyzes the dehydration of versiconal to versicolorin B or versicolorin C [versicolorin B(C)]. The enzyme was purified from mycelia of Aspergillus parasiticus by DEAE-cellulose, hydroxylapatite, and Mono Q column chromatography. The protein contains two identical subunits of molecular weight 72,00...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90366-5

    authors： Lin BK,Anderson JA

    更新日期：1992-02-14 00:00:00

  • Mechanisms of inhibition of phenylalanine ammonia-lyase by phenol inhibitors and phenol/glycine synergistic inhibitors.

    abstract：:Phenylalanine ammonia-lyase (PAL) catalyzes the beta-elimination of ammonia from L-phenylalanine to trans-cinnamic acid. A study of inhibition of PAL by phenol, ortho-cresol, and meta-cresol gave mixed inhibition; para-cresol is not an inhibitor. The calculated values of K(i) and alphaK(i) are phenol, K(i)=2.1+/-0.5 m...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(03)00007-9

    authors： Alunni S,Cipiciani A,Fioroni G,Ottavi L

    更新日期：2003-04-15 00:00:00

  • The effect of essential fatty acid deficiency on the adrenergic activation of glycogenolysis in rat hepatocytes.

    abstract：:The fatty acid composition of total lipids and the adrenoceptor-mediated activation of glycogenolysis were studied in isolated hepatocytes from rats maintained on a control diet or on an essential fatty acid (EFA)-free diet. In cells from rats on the EFA-free diet there was a marked reduction in linoleic and arachidon...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(90)90608-2

    authors： Grojec MS,Ishac EJ,Kapocsi J,Kunos G

    更新日期：1990-11-15 00:00:00

  • Interaction of bovine glutamate dehydrogenase with isoquinoline alkaloids.

    abstract：:Representatives of four different classes of isoquinoline alkaloids (aporphines, benzylisoquinolines, tetrahydroisoquinolines, and phthalideisoquinolines) inhibit glutamate dehydrogenase activity in both reaction directions. Representatives of five other groups of isoquinoline alkaloids have been without any significa...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90144-3

    authors： Kovár J

    更新日期：1983-02-15 00:00:00

  • Kinetics of human alcohol dehydrogenase with ring-oxidized retinoids: effect of Tween 80.

    abstract：:Human alcohol dehydrogenases (ADH1 and ADH4) actively use retinoids oxidized at the cyclohexenyl ring (4-oxo-, 4-hydroxy-, and 3,4-didehydro-retinoids), which are functional compounds in several cells and tissues (i.e., in human skin). Remarkably, activities with 4-oxo-retinal and 4-hydroxy-retinol (kcat = 2050 min(-1...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2004.07.002

    authors： Martras S,Alvarez R,Gallego O,Domínguez M,de Lera AR,Farrés J,Parés X

    更新日期：2004-10-15 00:00:00

  • Involvement of cutaneous SR-B1 in skin lipid homeostasis.

    abstract：BACKGROUND:The main functions of the skin are to protect against environmental insults and prevent water loss, which are performed by the complex lipid- and protein matrix present in the outermost layers of the epithelium. The lipidome of these outer layers is mainly composed of ceramides, fatty acids, and cholesterol,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.03.005

    authors： Muresan XM,Narzt MS,Woodby B,Ferrara F,Gruber F,Valacchi G

    更新日期：2019-05-15 00:00:00

  • Examination of the role of Gln-158 in the mechanism of CO(2) hydration catalyzed by beta-carbonic anhydrase from Arabidopsis thaliana.

    abstract：:We have cloned and overexpressed a variant of Arabidopsis thaliana beta-carbonic anhydrase (Q158A) that deletes the functional equivalent of the backbone amide NH of Thr-199 in human alpha-carbonic anhydrase II. The latter residue is hypothesized to be important in catalyzing the rate of CO(2)(-) HCO (3)(-) interconve...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2004.02.033

    authors： Rowlett RS,Tu C,Murray PS,Chamberlin JE

    更新日期：2004-05-01 00:00:00

  • Holo- and apo-cystalysin from Treponema denticola: two different conformations.

    abstract：:Cystalysin, the key virulence factor in the bacterium Treponema denticola responsible for periodontis, is a pyridoxal 5'-phosphate (PLP) enzyme which catalyzes, in addition to alpha,beta-elimination of L-cysteine, racemization and transamination of both enantiomers of alanine. In this paper several indicators have bee...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2006.08.020

    authors： Cellini B,Montioli R,Bossi A,Bertoldi M,Laurents DV,Voltattorni CB

    更新日期：2006-11-01 00:00:00

  • Transmembrane domain interactions affect the stability of the extracellular domain of the human NTPDase 2.

    abstract：:Human NTPDase2 and chicken NTPDase8 are cell surface nucleotidases that contain two transmembrane domains (TMD) and five apyrase conserved regions (ACRs). ACR1 is located near the N-terminal TMD whereas ACR5 is located near the C-terminal TMD. The human NTPDase2 activity is decreased by low concentration of NP-40 and ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.02.011

    authors： Chiang WC,Knowles AF

    更新日期：2008-04-15 00:00:00

  • Mixed function oxidation and enzymes: kinetic and structural properties of an oxidatively modified alkaline phosphatase.

    abstract：:No major structural alteration of alkaline phosphatase can be observed in the early stages of enzyme oxidative inactivation by the ascorbate model system. Fluorescence changes of protein-bound 8-anilino-1-naphthalenesulfonic acid suggest, however, that localized modifications take place. Oxidized alkaline phosphatase ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90315-3

    authors： Mordente A,Martorana GE,Miggiano GA,Meucci E,Santini SA,Castelli A

    更新日期：1988-08-01 00:00:00