Abstract:
:The gene encoding the major envelope glycoprotein of the HIV-SF2 isolate was engineered for the secretion of recombinant gp120 (rgp120SF2) from permanent Chinese hamster ovary (CHO) cell lines. Cellular production methods were scaled up and a method for purification of the secreted glycoprotein was devised. Mild purification conditions were selected in order to preserve the native structure of the protein. rgp120SF2 exhibits a molecular weight of 120 kDa in reduced or nonreduced SDS gels; thus the polypeptide chain is intact. Deglycosylated rgp120SF2 has the predicted molecular weight of the polypeptide backbone, 54 kDa. Gel-filtration HPLC in a nondenaturing buffer at neutral pH yields a molecular weight estimate of approximately 120 kDa. Purified rgp120 closely resembles authentic viral gp120 by several physical, chemical, and immunochemical tests. rgp120SF2 reacts strongly with human HIV-positive sera, monoclonal antibodies reactive with HIV-SF2 and HIV-MN viral envelope, and a human virus-neutralizing monoclonal antibody that maps to a conserved discontinuous epitope on HIV-1 gp120. Purified rgp120SF2 forms a 1:1 molecular complex with soluble recombinant human CD4 (rCD4) receptor, as demonstrated by gel-filtration HPLC; binding is high affinity (Kd approximately 2 x 10(-9) M).
journal_name
AIDS Res Hum Retrovirusesjournal_title
AIDS research and human retrovirusesauthors
Scandella CJ,Kilpatrick J,Lidster W,Parker C,Moore JP,Moore GK,Mann KA,Brown P,Coates S,Chapman Bdoi
10.1089/aid.1993.9.1233subject
Has Abstract,Author List Incompletepub_date
1993-12-01 00:00:00pages
1233-44issue
12eissn
0889-2229issn
1931-8405journal_volume
9pub_type
杂志文章abstract::Interferon-α (IFN-α) plays a vital role in combating viral infections especially in the early control after infection. However, the HIV infection has shown substantial level of suppression of IFN-α secretion during initial phase of infection. The reasons behind this impairment are still obscure. As plasmacytoid dendri...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1996.12.303
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1992.8.565
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journal_title:AIDS research and human retroviruses
pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2008-02-01 00:00:00
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1987.3.157
更新日期:1987-07-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1996.12.1161
更新日期:1996-08-10 00:00:00
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pub_type: 杂志文章,随机对照试验
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1995.11.1467
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1998.14.299
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2014.0124
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章,多中心研究
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1996.12.223
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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