Abstract：
:Because metallothionein (MT) may undergo thiol-disulfide or other redox reactions under certain cellular conditions, the partially and completely oxidized products of the reactions of Cd7-MT-II with the electrophile 5,5'-dithiobis(2-nitrobenzoic acid), ESSE, and oxidized glutathione, GSSG, were characterized. Reaction with the stoichiometric quantity of ESSE (1 ESSE per MT thiolate) generates monomeric and polymeric MTs with three types of disulfide bonds: intra- and intermolecular CyS-SCy linkages and a small number (2-3/MT) of mixed disulfides, CyS-SE, involving thionitrobenzoate (ES-). Reaction with substoichiometric quantities of ESSE (0.02 or 0.1 per MT thiolate) causes the formation of intra- and intermolecular CyS-SCy disulfides, but no mixed disulfides. In the latter reactions, two equivalents of ES- are released per mole of ESSE, but the release is described by a single first-order rate constant (k = 3.0 +/- 0.5 sec-1). Substantial amounts of cadmium remained bound to the MT monomers and polymers after reaction with the substoichiometric quantities. Despite the Cd bound to the MT after reaction with 0.1 ESSE per MT thiolate, no 111Cd NMR signals were detected, indicating rapid equilibration of the remaining metal ions among the disrupted binding sites. Large excesses of the endogenous aliphatic disulfide, GSSG, displace Zn+2 from Zn7-MT slowly. The reaction is complete after 24 hours with 5000 microM GSSG, but only 25% complete after 72 hours with 250 microM GSSG. Approximately one Cd+2 is displaced rapidly from Cd7MT by 5000 microM GSSG and half as much by 250 microM GSSG, but no further reaction occurs. It is unlikely that GSSG oxidation of MTs would be physiologically significant.
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
Savas MM,Shaw CF 3rd,Petering DHdoi
10.1016/0162-0134(93)80028-8subject
Has Abstractpub_date
1993-12-01 00:00:00pages
235-49issue
4eissn
0162-0134issn
1873-3344pii
0162-0134(93)80028-8journal_volume
52pub_type
杂志文章abstract：:Development of antibiotic resistance in bacteria causes major challenges for our society and has prompted a great need for new and alternative treatment methods for infection. One promising approach is to target bacterial virulence using for example salicylidene acylhydrazides (hydrazones). Hydrazones coordinate metal...
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
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journal_title：Journal of inorganic biochemistry
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2017.04.020
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2016.02.030
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章,评审
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/0162-0134(88)85015-3
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2010.11.004
更新日期：2011-02-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2017.11.016
更新日期：2018-02-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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更新日期：2002-04-10 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
abstract：:Three vanadium complexes of chlorodipicolinic acid (4-chloro-2,6-dipicolinic acid) in oxidation states III, IV, and V were prepared and their properties characterized across the oxidation states. In addition, the series of hydroxylamido, methylhydroxylamido, dimethylhydroxylamido, and diethylhydroxylamido complexes we...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2008.12.015
更新日期：2009-04-01 00:00:00
abstract：:Hexacyanochromate ion, (Cr(CN)6)3-, was applied to ribonuclease T1 (RNase T1), which specifically cleaves RNA chains at guanylic acid residues. From kinetic studies, this anion was shown to bind to the active site of RNase T1 as a competitive inhibitor. Therefore, the line broadening effect of NMR resonances due to bi...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/0162-0134(86)80095-2
更新日期：1986-10-01 00:00:00
abstract：:Aluminum salts such as aluminum chlorohydrate (ACH) are known for use as an active antiperspirant agent that blocks the secretion of sweat. A local case report of hyperaluminemia in a woman using an aluminum-containing antiperspirant for 4 years raises the problem of transdermal absorption of aluminum (Al). Only a ver...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2012.02.013
更新日期：2012-05-01 00:00:00
abstract：:Transition metals, such as Cu(+2), Mn(+2), and Co(+2), have been seen to catalyze the bicarbonate enhanced oxidation of a variety of substrates by H(2)O(2). In several of these cases it has been demonstrated that CO(2), rather than bicarbonate, is the enhancing species. Mechanisms that are in accord with the data invo...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.jinorgbio.2006.01.030
更新日期：2006-04-01 00:00:00
abstract：:Phosphate buffer suspensions of unilamellar liposomes of phosphatidylcholine were irradiated with UVC (254 nm) and UVB (300 nm) light. The irradiation provoked lipid peroxidation and liposome lysis with release of entrapped glucose-6-phosphate. At the same intensity of absorbed light, the photochemical effect at 254 n...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/0162-0134(94)00048-f
更新日期：1995-07-01 00:00:00
