Abstract:
:The sodium-ion coupled transporters for 5-hydroxytryptamine (5HT), noradrenaline and dopamine function to reduce extracellular levels of biogenic amines. Over the past fifteen years selective inhibitors of these transport systems have been developed including fluoxetine, citalopram, paroxetine, litoxetine (for 5HT), nisoxetine, desipramine, maprotiline (for noradrenaline) and GBR-12935 (for dopamine). Some of these inhibitors, including drugs selective for noradrenaline transport and particularly those selective for the 5HT transport system are currently widely used in the clinical management of affective disorders. Selective biogenic amine uptake inhibitors have, in addition, provided tools to undertake molecular pharmacological and biochemical studies of their respective transporters. By this means, the rat brain 5HT and dopamine transporters have been identified as polypeptides with relative molecular masses of 73,000 and 80,000, respectively, using affinity-chromatographic purification and photoaffinity-labelling techniques. Recently, the biogenic amine transporters have been cloned and a comparison of their predicted amino acid sequences reveals that these proteins share a considerable degree of similarity with notably 12-13 transmembrane spanning domains. Perspectives for future fundamental and clinical research on biogenic amine transport systems using molecular biological techniques are discussed.
journal_name
Life Scijournal_title
Life sciencesauthors
Graham D,Langer SZdoi
10.1016/0024-3205(92)90236-isubject
Has Abstractpub_date
1992-01-01 00:00:00pages
631-45issue
9eissn
0024-3205issn
1879-0631pii
0024-3205(92)90236-Ijournal_volume
51pub_type
杂志文章,评审相关文献
LIFE SCIENCES文献大全abstract::Bu-Zhong-Yi-Qi-Tang (BZYQT), a Chinese herbal medicine, inhibited the proliferation of human hepatoma cell lines (Hep3B, HepG2 and HA22T) dose-dependently. The IC50s of BZYQT on the proliferation of Hep3B, HepG2 and HA22T were 432.5+/-31.8 microg/ml, 455.4+/-24.2 microg/ml, and 2284.3+/-77.2 microg/ml respectively on ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01226-7
更新日期:2001-08-17 00:00:00
abstract::Noradrenaline is taken up in noradrenergic neurones via a desipramine-sensitive transport system (uptake1). There are reports which describe the activity of uptake1 being increased by extracellular ATP and ATP gamma S. This was attributed to a protein kinase-mediated mechanism. We reevaluated this phenomenon since a m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00665-2
更新日期:1994-01-01 00:00:00
abstract::The analgesic buprenorphine hydrochloride (Bph) induced apoptosis-like cell death in the caspase-3-deficient human breast cancer cell line, MCF-7. This apoptosis-like cell death activated key molecules in the mitochondrial apoptotic pathway: cytochrome c, caspase-9, caspase-7, and caspase-6. Bph caused the release of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.11.032
更新日期:2004-06-04 00:00:00
abstract::The effect of triiodothyronine (T3) on Na+,K(+)-ATPase activity of K562 human erythroleukemic cell was studied to understand why the erythrocyte sodium pump activity is decreased in hyperthyroidism. Na+,K(+)-ATPase activity of K562 cell lysates was assayed by measuring the release of inorganic phosphate (Pi) from ATP....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90107-z
更新日期:1992-01-01 00:00:00
abstract::Responses to [Mpr14]-ADM(14-50), a novel analog of adrenomedullin, were investigated in the hindlimb vascular bed of the cat under conditions of controlled blood flow. Intraarterial injections of [Mpr14]-rADM(14-50) in doses of 0.003-1 nmol caused dose-related decreases in hindlimb perfusion pressure. In terms of rela...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00258-5
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:To investigate the expression and time-dependent manner of bradykinin (BK) as well as its receptors (Bradykinin receptors 1 and 2, B1R and B2R) in spinal cord ischemia-reperfusion injury (SCII) in rat model. METHODS:Sprague-Dawley (SD) rats were subjected to 1 h of infra-renal abdominal aorta occlusion and r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.12.034
更新日期:2019-02-01 00:00:00
abstract::Rats implanted subcutaneously with an empty osmotic pump connected by a polyethylene catheter to a jugular vein for 5 to 10 days evinced a decreased splenocyte responsiveness to blastogenic stimulation in vitro to bacterial lipopolysaccharide, a known B cell stimulator, as well as to the plant mitogens pokeweed mitoge...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90291-v
更新日期:1992-01-01 00:00:00
abstract::The concentrations of the trace amines, m-tyramine, p-tyramine, phenylethylamine and tryptamine, were measured in the striatum of the brain and in the kidney of adult rats treated with alpha-monofluoromethyldopa (MFMD), an inhibitor of aromatic amino acid decarboxylase. While MFMD decreased the levels of all four amin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90371-7
更新日期:1987-02-09 00:00:00
abstract::The inhibitory activity of methylguanidine, guanidine and their precursors, creatine and creatinine, on both the neuronal constitutive and lung inducible isoforms of nitric oxide synthase were examined in this study. Methylguanidine and guanidine (0.01-3 mM) significantly (P<0.01) inhibited in a concentration-dependen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00673-5
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVE:To investigate the protective efficacies and potent mechanisms of combination therapy with semaglutide and rosiglitazone (RSG) on the high-glucose incubated human ARPE-19 cells and diabetic retinopathy (DR) model rats. MAIN METHODS:The CCK-8 methods were used to evaluate the protective effects of semaglutide...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.119013
更新日期:2021-01-05 00:00:00
abstract::Human, dog and rabbit corpus cavernosum type 5 phosphodiesterases (PDE5) were isolated and their characteristics were compared. The three enzymes showed Km values of 0.8, 2.1 and 2.3 uM, respectively. They exhibited similar pH-dependence with optimal pH being 7.5. They required Mg++ for activity and the activity was s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00989-4
更新日期:2001-03-16 00:00:00
abstract::5-Bromo-2'-deoxyuridine (BrdU) has been recently found to enhance adrenal glucocorticoid secretion in rats. Since BrdU-labeling is today the most employed technique for assessing cell proliferation, we have investigated whether this side-effect of BrdU may influence mitotic activity (as evaluated by metaphase-arrested...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00439-6
更新日期:1997-01-01 00:00:00
abstract::Initial acute behavioral studies in mice indicated that phencyclidine (PCP) produced marked motor impairment as measured by the inverted screen technique with an ED50 value of 4.1 muMole/kg (i.v.). Phenylcyclohexene (PC) was considerably less active with an ED50 value of 325 muMole/kg (i.v.). PCP was also shown to be ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90708-1
更新日期:1982-08-23 00:00:00
abstract:AIMS:Extensive evidence supports a role for voltage-gated calcium channels (VGCC) and TRPV1 receptors in pain transmission and modulation. We investigated the profile of analgesic interaction between Phα1β toxin (a VGCC blocker) and SB366791 (selective TRPV1 antagonist) in a model of acute pain induced by capsaicin. Ch...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.06.018
更新日期:2017-08-01 00:00:00
abstract:AIMS:3-Nitropropionic acid (3-NPA) is a naturally occurring fungal toxin that leads to ATP-depletion by inhibiting mitochondrial succinate dehydrogenase and produces chemical anoxia. The present study was conducted to identify the involvement of inhibitory system in 3-NPA-induced depression of spinal reflexes. METHODS...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.09.017
更新日期:2008-11-21 00:00:00
abstract::A number of potential functions of thioredoxin have been proposed in literature, including a role for DNA replication. The aim of our study was to investigate the effects of thioredoxin from Streptomyces aureofaciens (Trx S.a.) on plasmid DNA. Trx S.a. was incubated with plasmid forms and the incubation product(s) cha...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)01133-8
更新日期:1998-01-01 00:00:00
abstract::Specific 125I-CCK receptor binding was significantly increased in brain tissue taken from guinea pig or mouse following chronic (2-3 week) daily administration of haloperidol (2-3 mg/kg/day). Scatchard analysis indicated the increase in CCK binding was due to an increased receptor number (B max) with no change in affi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90224-2
更新日期:1983-02-21 00:00:00
abstract::Alpha adrenergic agonists and antagonists as clonidine, guanfacine, yohimbine, phenylephrine and prazosin inhibited the [3H]-QNB binding to rat brain cortex muscarinic acetylcholine receptor (mAChR, M-1 subtype), heart (M-2 subtype) and parotid gland homogenate (M-3 subtype) in a dose-dependent competitive fashion. Ki...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90436-u
更新日期:1990-01-01 00:00:00
abstract::The aim of this study was to examine the distribution of dopamine and norepinephrine in the proximal alimentary tract of the rat and to assess the contributions of sympathetic and vagal fibers to the tissue concentrations of both catecholamines. Tissues were extracted in perchloric acid and the catecholamines were sep...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90365-0
更新日期:1985-04-29 00:00:00
abstract::We investigated the effects of genistein and genistin on proliferation and apoptosis of human ovarian SK-OV-3 cells and explored the mechanism for these effects. SK-OV-3 cells were treated with genistein and genistin at various concentrations (ranging from 1 to 100 muM) either alone or in combination for 24 and 48 h. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.12.031
更新日期:2007-03-20 00:00:00
abstract::The aim of the present work was to study the effects of various durations of fasting (12, 24 and 72 hours) on brown adipose tissue (BAT) thermogenic parameters--cytrochrome-c-oxidase (COX) activity, GDP-binding activity and uncoupling protein (UCP1) content--and also on morphological features of different mitochondria...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00153-2
更新日期:1998-01-01 00:00:00
abstract:AIMS:To examine the effects of metoprolol on expression of myocardial inflammatory cytokines and myocardial function in rats following coronary microembolization (CME). MAIN METHODS:Male rats were randomly assigned to receive either sham-operation (control group), CME plus saline (CME group), or CME plus metoprolol (m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.03.012
更新日期:2011-06-06 00:00:00
abstract::Increasing understanding in tumor pathology reveals that tumor microenvironment (TME), which supports tumor progression and poses barriers for available therapies, takes a great responsibility in inefficient treatment and poor prognosis. In recent years, the versatile nanotechnology employed in TME regulation has made...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.03.044
更新日期:2018-05-15 00:00:00
abstract::Examination was made of the effects of 17 synthetic and naturally occurring flavonoids on human lymphocyte proliferation in the presence of concanavalin A as a mitogen. Twelve of the flavonoids examined were mono-hydroxy or methoxy derivatives. The mitogen-induced response of lymphocytes was evaluated from the extent ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90028-3
更新日期:1989-01-01 00:00:00
abstract:AIMS:The study aimed to investigate whether IL-23 is amplified in monocyte subsets of MP pneumonia and to determine its relevant pathway. MATERIALS AND METHODS:We firstly analyze the IL-23p19 expression in monocyte subgroups in MP pneumonia patients and healthy controls subjects by using flow cytometry. Then, we also ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118149
更新日期:2020-10-01 00:00:00
abstract::Gradual increases in the electrical stimulation of the inferior colliculus produces progressive aversive responses from vigilance, through freezing, until escape. These responses are probably mediated by excitatory amino acids (EAA) mechanisms as microinjection of glutamate into the inferior colliculus can trigger fre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02169-0
更新日期:2002-11-08 00:00:00
abstract::1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-O-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90129-9
更新日期:1987-04-20 00:00:00
abstract::CGS 10746B, a benzothiadiazepine, has a behavioral profile in mice and monkeys similar to the atypical antipsychotic clozapine. Unlike clozapine, CGS 10746B suppresses dopamine neuron firing rates and, when administered at behaviorally effective doses by the oral or intraperitoneal route, decreases neostriatal dopamin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90017-2
更新日期:1986-08-25 00:00:00
abstract::The effects of (-)-epigallocatechin-3-gallate (EGCG), the most abundant catechin of tea, on Ca(2+)-permeable non-selective cation currents (NSCC) and voltage-operated Ca(2+) channels (VOCC) have been investigated in cultured rat aortic smooth muscle cells using the whole-cell voltage-clamp technique. Under the Cs(+)/t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.04.005
更新日期:2007-05-16 00:00:00
abstract::Growth-related oncogene protein-alpha (GRO-alpha) is a member of the C-X-C chemokine family with a wide variety of biological activities. We studied the production of GRO-alpha by human umbilical vein endothelial cells (HUVEC) in response to the stimulation with soluble form of interleukin-6 receptor alpha (sIL-6R). s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01560-6
更新日期:2002-05-17 00:00:00