Abstract:
:Modified limiting dilution analysis (LDA) techniques were used to evaluate the mobilization of antigen-stimulated helper T lymphocytes (HTL) and cytotoxic T lymphocytes (CTL) following allogeneic heterotopic cardiac transplantation. These modified LDA techniques allow a quantitative comparison of T cells that have been stimulated by antigen in vivo versus unstimulated precursor T cells of the same antigen specificity. Endothelial changes associated with mononuclear cell infiltration of the transplant were studied using endothelia-specific monoclonal antibodies and immunohistochemistry. Early (day 3) infiltration of cardiac allografts was characterized by a prevalence of donor alloantigen-specific HTL over CTL. Immunohistology revealed that the day-3 infiltrate was associated with areas of differentiated vascular endothelium, located primarily in the subepicardial region. Though donor-specific precursor HTL and CTL were present in the peripheral lymphoid tissues and blood, very few of them had been stimulated at this early time. During the latter phases of the response (days 6-9), antigen-stimulated HTL and CTL were present in the rejecting heart with CTL dominating the response. Accumulation of large numbers of donor-specific CTL in the allograft correlated with extensive inflammatory endothelial development, myocyte destruction, and loss of graft function by day 9. Stimulated HTL and CTL were detectable in peripheral lymphoid tissues at days 6 and 9. In addition, a marked increase in the number of donor-specific precursor CTL, but not precursor HTL, was observed in the lymphoid tissues at the peak of the response. Depletion of class II MHC-restricted T cells by in vivo treatment with anti-CD4 mAb eliminated HTL activity in all lymphoid compartments assessed and markedly reduced the number of CTL infiltrating the allograft. In addition, no stimulated CTL were detectable in lymphoid tissues, and the number of precursor CTL was not increased. In anti-CD4-treated recipients, cardiac allografts remained functional with minimal histological evidence of rejection for at least 21 days. Though graft-associated inflammatory endothelia were absent in anti-CD4-treated recipients at day 6, endothelial differentiation was observed in day 21 allografts in anti-CD4-treated recipients. These observations indicate that inflammatory endothelial development may precede T cell infiltration and subsequent loss of the cardiac allograft function. Thus, CD4-positive HTL are required for (1) graft-associated inflammatory endothelial development; (2) CTL activation in peripheral lymphoid tissues; (3) CTL accumulation in allografted tissues; and (4) acute cardiac allograft rejection.
journal_name
Transplantationjournal_title
Transplantationauthors
Bishop DK,Shelby J,Eichwald EJsubject
Has Abstractpub_date
1992-04-01 00:00:00pages
849-57issue
4eissn
0041-1337issn
1534-6080journal_volume
53pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Osteoarthritis is the most prevalent joint disease and a frequent cause of joint pain, functional loss, and disability. Osteoarthritis often becomes chronic, and conventional treatments have demonstrated only modest clinical benefits without lesion reversal. Cell-based therapies have shown encouraging result...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318291a2da
更新日期:2013-06-27 00:00:00
abstract:BACKGROUND:The clinical significance of HLA-directed antibodies newly detected after transplantation (HT) is controversial. METHODS:Seventy-one HT recipients consented to enroll. Mean follow-up time was 28 months (range 6-48). Panel reactive antibody (PRA) analysis was performed on posttransplant sera (2 weeks, 1, 2, ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000180564.14050.49
更新日期:2005-10-27 00:00:00
abstract::Transplantation provides life-saving therapy to critically ill patients with end-stage organ failure. We found that the specialized knowledge of mental health clinicians and physicians, committed to the evaluation and management of transplant recipients, is essential for the optimal care of these patients who require ...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0b013e3181a754d4
更新日期:2009-06-27 00:00:00
abstract:BACKGROUND:Graft failure related to acute and chronic rejection remains an important problem in transplantation. An association has been reported between microchimerism and the development of tolerance. Since it has been established that cells of fetal origin can be found in maternal tissues long after parturition, and...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181705a0f
更新日期:2008-05-27 00:00:00
abstract::In an effort to determine the influence of immunosuppressive therapy and other clinical variables on posttransplant osteopenia, vertebral bone density was measured at least 6 months after transplantation in 65 adult primary renal transplant recipients receiving a variety of immunosuppressive regimens. Fifteen of the 6...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-12-15 00:00:00
abstract:BACKGROUND:The rate of acute rejection (AR) has decreased significantly, but whether this is associated with improvement in long-term graft survival is controversial. METHODS:We analyzed 1445 consecutive adult deceased donor kidney transplant recipients from 1985 to 2005, over two periods (1985-1995 vs. 1996-2005) to ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181c892dd
更新日期:2010-03-27 00:00:00
abstract:BACKGROUND:Finding the best combination of immunosuppression is an important challenge in kidney transplantation. Current short-term (1- and 3-year) allograft survival is quite good, making it difficult to determine differences in therapeutic regimens without large sample sizes. Using data from the United Network for O...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000079839.38844.09
更新日期:2003-08-15 00:00:00
abstract::The association of renal vein thrombosis and membranous glomerulopathy in native kidneys has been well documented. However, this association has only occasionally been described following renal transplantation. A review of 693 renal transplant recipients revealed 77 (11%) in whom persistent, heavy proteinuria (greater...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::KM2210, a conjugate of estradiol and chlorambucil (CBL), which was originally developed as an anti-breast cancer agent, inhibits proliferative response of human mononuclear cells to alloantigens in mixed lymphocyte culture in a dose-dependent manner, but has no effect on their response to phytohemagglutinin. Neither e...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199210000-00022
更新日期:1992-10-01 00:00:00
abstract:BACKGROUND:In nontransplant patients with chronic hepatitis C virus (HCV), HCV genotype has been linked with a differential response to antiviral therapy, risk of steatosis and fibrosis, as well as all-cause mortality, but the role of HCV genotypes in posttransplant disease progression is less clear. METHODS:Using the...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0000000000000413
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:This study aimed to determine factors associated with body composition changes shortly after liver transplantation (LTx), including the influence of immunosuppressive agents. The combined resting energy expenditure (REE) and handgrip strength provided a valuable assessment in data interpretation of body comp...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001202
更新日期:2016-08-01 00:00:00
abstract::Vascular endothelial cells (VEC) are clearly immunogenic and express antigens unique to vascular endothelial cells. The observation that peripheral blood monocytes also express this VEC antigen system made prospective testing feasible. Preformed antibody to the VEC/monocyte antigen system of the donor usually leads to...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198503000-00016
更新日期:1985-03-01 00:00:00
abstract:BACKGROUND:Renal dysfunction early after kidney transplantation has multiple causes including ischemia-reperfusion (I/R) injury and drug-induced nephrotoxicity. This study assesses the acute nephrotoxicity of tacrolimus (Tac) and sirolimus (Sir) in a rat renal isograft model. METHODS:Lewis renal isografts and uninephr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000128837.07640.ae
更新日期:2004-08-15 00:00:00
abstract:BACKGROUND:Apoptosis has been identified after ischemia-reperfusion (IR) injury to the brain, heart, kidney, retina, and the adrenals. Intestinal IR injury causes villous and crypt damage, which has so far been attributed to cellular necrosis. This study was undertaken to investigate the possible role of apoptosis afte...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199711270-00003
更新日期:1997-11-27 00:00:00
abstract::LEW rats (RT1l) that are lethally irradiated and repopulated with syngenic bone marrow accept WF (RT1u) cardiac allografts. If bone marrow repopulation is delayed for two days after irradiation and operation, grafts containing passenger leukocytes (nonperfused grafts) are generally rejected, but perfused grafts, which...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198404000-00013
更新日期:1984-04-01 00:00:00
abstract:BACKGROUND:We have previously shown that CD8 depletion or CD34 selection of peripheral blood stem cells (PBSC) reduced the incidence of acute graft-versus-host disease (GvHD) after nonmyeloablative stem-cell transplantation (NMSCT). In this study, we analyze the effect of CD8 depletion or CD34 selection of the graft on...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000093987.11389.F7
更新日期:2003-12-27 00:00:00
abstract::The mechanism (or mechanisms) underlying the indefinite survival of DBA/2 islet allografts in strongly histoincompatible (C57BL/6xA)F1 (B6AF1) mice, induced either by the combined use of Ficoll-prepared crude islets and treatment of recipients with antilymphocyte serum (ALS), or by the use of handpicked, purified isle...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198802000-00037
更新日期:1988-02-01 00:00:00
abstract::Of 2457 patients in the North American Pediatric Renal Transplant Cooperative Study registry who were followed for 5481 patient-years after the index transplantation, we observed 136 deaths, for an average annual rate of 24.8 deaths per 1000 patient-years. Death resulted primarily from infection (n = 55, 40%), cardiov...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:
更新日期:1994-02-27 00:00:00
abstract::Reports of early success with cryopreserved saphenous veins (CSV) as arterial conduits led us to develop cryopreserved iliac veins (CIV) as interposition grafts for portal vein reconstruction in living-related liver transplantation (LRLT). Despite encouraging short-term results, retrospective analysis of long-term cry...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/00007890-199609270-00007
更新日期:1996-09-27 00:00:00
abstract::Donor pancreas utilization rates for whole organ transplant have remained low and have decreased over time. To identify the reasons for nonuse of pancreas from donors who meet common baseline acceptance criteria, we examined Organ Procurement and Transplantation Network data from 2005 to 2007 and identified a subgroup...
journal_title:Transplantation
pub_type: 社论,评审
doi:10.1097/TP.0b013e3181e9fea4
更新日期:2010-09-15 00:00:00
abstract::First cadaver, unrelated kidney graft survival at one year from 50 Eurotransplant centers was analyzed and found to show marked changes in survival rates over the 20-year period from 1967 to 1986 inclusive. Full information on HLA-A, -B, and -DR matching and use of cyclosporine therapy was only available for the perio...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198811000-00012
更新日期:1988-11-01 00:00:00
abstract::When normal mice are exposed for short periods to ultraviolet light (UV), they support the progressive growth of transplanted syngeneic UV-induced tumors. Normal nonirradiated mice almost always reject these tumor implants. The UV-mediated suppression of the antitumor response can be adoptively transferred to normal s...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197708000-00005
更新日期:1977-08-01 00:00:00
abstract::Though the high immunosuppressive efficacy of cyclosporine has revolutionized clinical transplantation, renal, hepatic, and neural toxicities limit its therapeutic potential. One approach to this problem is to combine CsA therapy with immunosuppressive agents that act synergistically with it, thereby permitting lower ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199002000-00044
更新日期:1990-02-01 00:00:00
abstract::Discordant xenogeneic organ transplantation is a potential solution to the critical shortage of suitable donor organs. However, clinical application of xenotransplantation with physiologically suitable organs such as those from the pig, is currently limited by the lack of agents to prevent antibody and complement-medi...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-05-27 00:00:00
abstract:BACKGROUND:The objectives of this study are to investigate the impact of cytomegalovirus (CMV) donor (D)/recipient (R) serostatus on kidney transplant outcomes in recipients who received CMV prophylaxis and to investigate the association of individual antiviral agents (acyclovir, ganciclovir, and valganciclovir) with o...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181f7c053
更新日期:2010-11-27 00:00:00
abstract::After small bowel transplantation, not only rejection but also graft-versus-host reaction (GVHR) may occur. Donor T lymphocytes, transferred together with the graft, are a prerequisite for the development of GVHR. So far, however, little is known about the effector mechanisms in acute GVHR. It can be assumed that not ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199608150-00007
更新日期:1996-08-15 00:00:00
abstract::Numerous studies have reported successful allotransplantation of hepatocytes. However, none have shown long-term correction of a liver-related metabolic defect. In this study, we used a method of regional hepatocyte transplantation and subsequent induction of transplanted cell proliferation by regeneration response in...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199609150-00008
更新日期:1996-09-15 00:00:00
abstract::The generation of Gal knockout (KO) pigs is likely to be an important advance in xenotransplantation. However, recent reports suggesting that expression of Gal may not be completely eliminated raise the possibility of a continuing anti-Gal immune response. The authors used a Gal-mismatched skin graft model to study ce...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000116420.17173.e4
更新日期:2004-03-15 00:00:00
abstract:BACKGROUND:Post-lung transplant infection is one of the leading causes of morbidity and mortality. The cause and incidence are similar in many series; however, infections such as Mycobacterium tuberculosis are influenced by the epidemiologic situation. The authors present a prospective and observational study to define...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000147784.53188.dc
更新日期:2005-01-15 00:00:00
abstract::Most patients with secondary hypertension due to renal disease or on maintenance hemodialysis have lost the physiologic fall of blood pressure during sleep. To test the notion that kidney transplantation normalizes the blood pressure profile, we monitored ambulatory blood pressure over 24 hr in 45 patients (29 males a...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-05-15 00:00:00