Abstract:
BACKGROUND:Apoptosis has been identified after ischemia-reperfusion (IR) injury to the brain, heart, kidney, retina, and the adrenals. Intestinal IR injury causes villous and crypt damage, which has so far been attributed to cellular necrosis. This study was undertaken to investigate the possible role of apoptosis after reperfusion of cold-stored small bowel grafts in syngeneic rats. METHODS:Small intestinal grafts were stored at 4 degrees C for 24 hr in saline (n=6) or in modified University of Wisconsin solution (n=6), followed by reperfusion for 1 hr in syngeneic Lewis rats. Small bowel samples were obtained before storage, after preservation and after 1 hr of reperfusion. They were processed for light and electron microscopy and analyzed for cell death, with particular emphasis on apoptosis. RESULTS:Less than one apoptotic event was seen per 10 crypts in normal and stored bowels. An occasional normal and some denuded villous epithelial cells of stored bowels exhibited apoptosis. After isotransplantation and 1 hr of reperfusion, marked increase in apoptosis was seen in the crypts and denuded villous epithelial cells of both saline- and modified University of Wisconsin-stored bowels. Secondary necrosis was seen in apoptotic cells, as were dark cells. Only a few cells showed signs of primary ischemic necrosis. CONCLUSIONS:Apoptosis occurs after intestinal IR injury. Modulation of its genetic regulatory and biochemical effector machinery might alleviate or even prevent IR injury in small bowel transplanted after similar periods of storage.
journal_name
Transplantationjournal_title
Transplantationauthors
Shah KA,Shurey S,Green CJdoi
10.1097/00007890-199711270-00003subject
Has Abstractpub_date
1997-11-27 00:00:00pages
1393-7issue
10eissn
0041-1337issn
1534-6080journal_volume
64pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::The influence of race on the outcome of cadaver renal transplantation (CRT) continues to be controversial even in the cyclosporine era. The present study examines the effect of race in 343 adult CRT performed from 1/1/82 through 10/1/88 with regard to the incidence of delayed function (DF), graft survival (GS), and pa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199001000-00014
更新日期:1990-01-01 00:00:00
abstract:INTRODUCTION:Celsior (CEL) was formulated specifically for heart preservation. Recently some preliminary reports have suggested that CEL is also effective for liver preservation. In this study liver preservation with CEL was compared to colloid-free University of Wisconsin solution (MUW). METHODS:Arterialized rat live...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200010270-00002
更新日期:2000-10-27 00:00:00
abstract:BACKGROUND:We hypothesized that tissue-engineered contractile cardiomyocyte sheets without a scaffold would show histological and electrical integration with impaired myocardium, leading to the regeneration of infarcted myocardium. METHODS:Neonatal rat cardiomyocytes were cultured on Poly(N-isopropylacrylamide)-grafte...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000181163.69108.dd
更新日期:2005-12-15 00:00:00
abstract::To investigate the causes of impaired liver function (LF)* after BMT, 88 patients were included for analysis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, transplant methods, preconditioning regimens, and graft-versus-host disease (GVHD). Fifty of them (56.8%) developed abnormal LF after BMT and a...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-04-27 00:00:00
abstract::Two anti-H-2Ld sera were analyzed, BALB/c-H-2dm2 anti-BALB/cBy and (C3H X BALB/c-H-2dm2)F1 anti-BALB/cHe, the latter also containing anti-Qa antibodies. Their reaction patterns were compared with an anti-Qa serum (C3H X BALB/cBy)F1 anti-BALB/cHe. Four H-2 specificities could be detected by the anti-H-2Ld sera, two alr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198108000-00009
更新日期:1981-08-01 00:00:00
abstract:BACKGROUND:Tacrolimus (TAC) is the cornerstone of immunosuppressive regimen in liver transplantation (LT). Its pharmacokinetics is characterized by a high interpatient and intrapatient variability (IPV) leading to an unpredictable dose-response relationship. The aim of our study was to evaluate the impact of TAC IPV (I...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002052
更新日期:2018-03-01 00:00:00
abstract::The aim of this study was to assess the efficacy of an enteral diet fortified with arginine, RNA, and fish oil (Impact), alone and in combination with cyclosporine (CsA) and donor-specific transfusion (DST) on allograft survival in the ACI:Lewis rat cardiac transplant model. Animals were fed ad libitum with either sta...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-10-27 00:00:00
abstract:BACKGROUND:Kidney donation after cardiac death leads to vascular damage as a result of warm ischemia, affecting renovascular circulating volume. Novel ultrasound dilution techniques may be used to measure renovascular circulating volumes during hypothermic machine perfusion of donor kidneys. METHODS:Renovascular circu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31828a46f7
更新日期:2013-05-15 00:00:00
abstract:BACKGROUND:Natural antibodies that bind the carbohydrate antigen Galalpha1-3Galbeta1-4GlcNAc-R (alphaGal) mediate rigorous rejection of porcine xenografts and represent a major immunological hurdle to successful discordant xenotransplantation. However, little is known about how production of antibodies specific for alp...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200205270-00007
更新日期:2002-05-27 00:00:00
abstract:BACKGROUND:Prophylactic ganciclovir (GCV) is used in high-risk renal transplant patients to prevent acute cytomegalovirus (CMV) disease, but its impact on inflammation within the allograft itself remains undefined. METHODS:To study the effect of GCV prophylaxis on allograft inflammation, murine CMV (MCMV)-infected all...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31822c6e89
更新日期:2011-10-15 00:00:00
abstract::Pancreas transplantation utilizing portal venous and enteric exocrine drainage has potential benefits over the standard systemic venous and bladder exocrine drainage method. Unfortunately, technical difficulties are often experienced with the arterial anastomosis after the portal venous anastomosis is completed. We ha...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199907270-00029
更新日期:1999-07-27 00:00:00
abstract::Ultraviolet (UV)-irradiated mice were compared with unirradiated mice for their susceptibility to primary and transplanted tumors etiologically unrelated to UV radiation. Although UV-irradiated mice are unable to reject transplants of highly antigenic syngeneic tumors induced by UV light, the growth of syngeneic, non-...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197909000-00012
更新日期:1979-09-01 00:00:00
abstract:BACKGROUND:Acid sphingomyelinase knock-out (ASMKO) mice are a model of types A and B Niemann-Pick disease. In the present study, we evaluated whether bone marrow transplantation (BMT) carried out on newborn ASMKO mice could prevent or alter the Niemann-Pick disease phenotype. METHODS:Previous work from our laboratory ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199804150-00005
更新日期:1998-04-15 00:00:00
abstract::Between December 1983 and August 1985, 110 cadaver transplants were performed at our institution. All were started on cyclosporine (CsA) and prednisone (P) for immunosuppressive therapy. Of the 110 patients, 46 were converted from CsA to azathioprine (AZA) for a variety of reasons (cost, toxicity, patient preference, ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198702000-00012
更新日期:1987-02-01 00:00:00
abstract:BACKGROUND:The effect of height and sex on liver transplantation (LT) for hepatocellular carcinoma (HCC) remains unclear. METHODS:Using United Network for Organ Sharing (UNOS) data, 14 844 HCC patients listed for LT from 2005 to 2015 were identified. Cumulative incidence of waitlist events (LT and dropout for death or...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0000000000002975
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:Human cytomegalovirus (CMV) uses different strategies to escape from human host defense reactions. Previously we have observed that infection of endothelial cells with CMV in vitro leads to enhanced activity of endothelial ectonucleotidases. These ectoenzymes are responsible for hydrolysis of extracellular a...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000287598.25493.a5
更新日期:2007-11-27 00:00:00
abstract:BACKGROUND:Donor organ shortage represents a major problem in lung transplantation. Donation after cardiac death could help to expand the pool of organs, but the additional period of warm ischemia after cardiac arrest aggravates primary graft dysfunction. The pulmonary endothelium of the graft constitutes an important ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318226bc6b
更新日期:2011-08-27 00:00:00
abstract::We have previously demonstrated that liver sinusoidal endothelial cells (LSECs) that endocytose portally injected allogeneic cells exert immunosuppressive effects on T cells with indirect allospecificity. In this study, we have demonstrated that invariant natural killer T cells plays a significant role in such immunos...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31816a3372
更新日期:2008-04-15 00:00:00
abstract::A sensitive micromethod for generating and assaying allogeneically induced cytotoxic human lymphocytes in vitro is described. Responding lymphocytes are cultured with mitomycin-C treated allogeneic stimulating cells in wells of replicate microtrays for both one-way mixed leukocyte culture (MLC) and cell-mediated lymph...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197606000-00005
更新日期:1976-06-01 00:00:00
abstract::Islet transplantation is currently being explored as a treatment for patients with type 1 diabetes. At present, the number of patients becoming insulin-independent is rapidly increasing world-wide applying the transplantation protocol originally described by the group in Edmonton. A hallmark in this procedure is repea...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000157273.60147.7c
更新日期:2005-05-27 00:00:00
abstract::Living organ donation is of increasing importance to satisfy the demand of good quality organs for patients remaining for extended periods on the waiting list. While the benefit for the recipient is undeniable, the organ procurement represents an important burden to live donors in terms of invasiveness and long-term c...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000284585.12633.06
更新日期:2007-10-15 00:00:00
abstract:BACKGROUND:Recent studies have described submandibular gland allografts in animal models; however, the amount of saliva secretion or nervous regeneration in those animals have not been reported. Herein, we investigated saliva secretion from submandibular gland allografts in beagle dogs. METHODS:Using a vascularized su...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000258598.27904.d7
更新日期:2007-03-27 00:00:00
abstract::Natural anti-carbohydrate antibodies are central to hyperacute rejection in ABO-incompatible allotransplantation and in discordant xenotransplantation. ABO-incompatible rejection has been inhibited successfully using intravenous soluble carbohydrates as antibody inhibitors. The approach has been less successful previo...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-12-15 00:00:00
abstract:BACKGROUND:The authors investigated the relationship between therapeutic blood transfusion before renal transplantation and rejection rates in cyclosporine- and tacrolimus-treated patients. METHODS:In one center, 265 consecutive recipients were studied. Protocol induction was with azathioprine, prednisolone, and cyclo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000111756.83834.A7
更新日期:2004-02-15 00:00:00
abstract:INTRODUCTION:This study examines the efficacy and toxicity of sirolimus used as primary immunosuppression in combination with reduced dose tacrolimus (calcineurin inhibitor [CI]-sparing regimen) or mycophenolate mofetil (CI-free regimen) in high-risk cadaveric renal transplantation. METHODS:Seventy subjects were treat...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/01.tp.0000121504.69676.5e
更新日期:2004-04-27 00:00:00
abstract::Leflunomide is a compound recently shown to reduce T and B cell-mediated responses in a number of experimental rat, mouse, and human systems. To explore its potential as an immunosuppressant, we studied leflunomide in 128 Brown-Norway/Lewis cardiac transplants and in 48 unoperated Lewis rats. At doses ranging from 0.6...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-04-27 00:00:00
abstract::Pulmonary thrombus formation in the region of atrial anastomosis following lung transplantation has been reported by several authors. Such patients typically present immediately after surgery with significant hemodynamic compromise causing pulmonary edema and hypoxemia. We describe a patient who presented with bilater...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199609150-00028
更新日期:1996-09-15 00:00:00
abstract::A comparative study of 24 hr preservation at 4 degrees C of excised rat livers with Euro-Collins and hydroxyethyl starch-free University of Wisconsin (UWm) solutions has been conducted based on the assessment of (1) the cellular energy status determined by 31P NMR spectroscopy and (2) cellular injury estimated from th...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199210000-00006
更新日期:1992-10-01 00:00:00
abstract::The value of percutaneous core needle biopsy in the immunohistological evaluation of renal allograft dysfunction was studied in 72 consecutive biopsies performed in 42 patients. The phenotypes of infiltrating cells mediating graft destruction were identified with monoclonal antibodies and immunoperoxidase staining tec...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198701000-00022
更新日期:1987-01-01 00:00:00
abstract::To characterize further lymphomas which arise in chronically immunosuppressed allotransplant patients, we studied the clinical and histological features of seven cases of nonHodgkin's lymphoma and determined the immunological phenotype in four cases, which developed in a series of 182 patients who underwent cardiac tr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198204000-00002
更新日期:1982-04-01 00:00:00