Abstract：
PURPOSE:A constituent of green tea, (-)-epigallocatechin-3-gallate (EGCG), has been known to possess anti-cancer properties. In this study, we investigated the time-course anticancer effects of EGCG on human ovarian cancer cells to provide insights into the molecular-level understanding of growth suppression mechanism involved in EGCG-mediated apoptosis and cell cycle arrest. METHODS:Three human ovarian cancer cell lines (p53 negative, SKOV-3 cells; mutant type p53, OVCAR-3 cells; and wild type p53, PA-1 cells) were used. The effect of EGCG treatment was studied via cell count assay, cell cycle analysis, FACS, Western blot, and macroarray assay. RESULTS:EGCG exerts a significant role in suppressing ovarian cancer cell growth. Also, EGCG showed growth inhibitory effects in each cell line in a dose-dependent fashion and induced apoptosis and cell cycle arrest. The cell cycle was arrested at the G(1) phase by EGCG in SKOV-3 and OVCAR-3 cells. In contrast, the cell cycle was arrested in the G(1)/S phase arrest in PA-1 cells. EGCG differentially regulated the expression of genes and proteins (Bax, p21, Retinoblastoma, cyclin D1, CDK4, Bcl-X(L)) more than 2-fold, showing a possible gene regulatory role of EGCG. The continual expression in p21WAF1 suggests that EGCG acts in the same way with p53 proteins to facilitate apoptosis after EGCG treatment. And Bax, PCNA, and Bcl-X are important in EGCG-mediated apoptosis. In contrast, CDK4 and Rb are not important in ovarian cancer cell growth inhibition. CONCLUSION:EGCG can inhibit ovarian cancer cell growth through induction of apoptosis and cell cycle arrest as well as regulation of cell cycle-related proteins. Thereby, the EGCG-mediated apoptosis can be applied to an advanced strategy in the development of a potential drug against ovarian cancer.
journal_name
Gynecol Oncoljournal_title
Gynecologic oncologyauthors
Huh SW,Bae SM,Kim YW,Lee JM,Namkoong SE,Lee IP,Kim SH,Kim CK,Ahn WSdoi
10.1016/j.ygyno.2004.05.031subject
Has Abstractpub_date
2004-09-01 00:00:00pages
760-8issue
3eissn
0090-8258issn
1095-6859pii
S0090825804003452journal_volume
94pub_type
杂志文章abstract：PURPOSE:The objective of this study was to determine the efficacy of high-dose-rate (HDR) brachytherapy in 36 medically inoperable patients with stage I endometrial cancer. METHODS AND MATERIALS:From October 1989 to August 1997, 36 patients presented with clinical stage I inoperable endometrial cancer. Surgery was pre...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1998.5148
更新日期：1998-11-01 00:00:00
abstract：:A patient with malignant struma ovarii who had elevated thyroglobulin levels at the time of her original surgery was diagnosed with recurrence on the basis of progressive thyroglobulin level elevation while on thyroid suppressive therapy. Subsequent treatment with therapeutic doses of iodine-137 resulted in normalizat...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1998.5056
更新日期：1998-09-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to profile cervical squamous cell carcinoma in elderly patients undergoing radiation therapy and to study the treatment outcome and side effects of therapy. MATERIALS AND METHODS:A retrospective analysis was carried out from the records of 380 patients with squamous cell carcino...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1999.5722
更新日期：2000-04-01 00:00:00
abstract：:An unusual case of Stage IB moderately well-differentiated endometrial adenocarcinoma that was confined to a polyp and recurred only in the abdominal wound is presented. Eighteen months following complete excision, local electron beam therapy, and the institution of hormonal therapy, the patient is alive and without o...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(86)90089-2
更新日期：1986-11-01 00:00:00
abstract：OBJECTIVES:Determinants of endometrial cancer grade have not been precisely defined, however, cell cycle control is considered to be integrally involved in endometrial cancer development. TP53 and MDM2 are essential components for cell cycle arrest and apoptosis. Polymorphisms in these genes cause TP53 inactivation and...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2008.12.036
更新日期：2009-04-01 00:00:00
abstract：:A low-molecular-weight glycoprotein containing sequences of the beta subunit of human chorionic gonadotropin (hCG) has been found in the urine of patients with carcinoma of the cervix using an immunoradiometric assay. This fragment has chromatographic and immunological identity with hCG beta core. This molecule was pr...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(90)90343-j
更新日期：1990-05-01 00:00:00
abstract：:This commentary is presenting opinions and advice on mentorship in the field of gynecologic oncology. Eleven academic gynecologic oncologists from across the country were interviewed and their thoughts about mentorship were recorded with recurring themes and ideas described in this commentary. Trust, respect, and mutu...
journal_title：Gynecologic oncology
pub_type： 社论
doi：10.1016/j.ygyno.2021.01.017
更新日期：2021-01-27 00:00:00
abstract：:Cyclin D1 is a critical gene controlling the G1 phase progression through the cell cycle. Alterations of cyclin D1 have been demonstrated in a variety of cancer types. We recently reported that increased cyclin D1 expression is associated with malignancy also in ovarian tumors. Three human ovarian cancer cell lines (S...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1998.5062
更新日期：1998-09-01 00:00:00
abstract：:Pelvic washings for cytologic analysis have become an accepted diagnostic procedure in the management of endometrial carcinoma. A retrospective study was performed in 163 consecutive patients with FIGO Stage I and II endometrial carcinoma to assess the significance and value of the washings. Abnormal results were obta...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/s0090-8258(87)80008-2
更新日期：1987-09-01 00:00:00
abstract：:Primary lymphoma of the cervix is very rare. A case report is presented and 24 cases published in the English literature are reviewed. Evaluation, staging, treatment, and prognosis of this rare tumor are discussed. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(87)90080-1
更新日期：1987-01-01 00:00:00
abstract：:Colposcopic cervical/vaginal examinations were performed on 10 healthy adult baboons. Five of the ten baboons had a "satisfactory" colposcopic cervical examination with columnar epithelium visible on the exocervix. However, only three of five with "satisfactory" colposcopic exams had large visible transformation zones...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(84)90216-6
更新日期：1984-03-01 00:00:00
abstract：OBJECTIVE:This study investigated the expression of cellular apoptosis susceptibility protein (CAS) in serous ovarian carcinoma by immunohistochemistry and compared it with topoisomerase IIalpha (topo IIalpha), bcl-2, bcl-x, the frequency of apoptotic bodies (ABI), mitotic activity, and c-erbB-2 with regard to clinicop...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2003.10.029
更新日期：2004-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the association between lymphopenia and survival in women with cervical cancer treated with primary chemoradiation. METHODS:A single institution, retrospective analysis of patients with stage IB2-IVA cervical cancer who received upfront chemoradiation from 1998 to 2013 was performed. Complete blo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2015.11.013
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:The presence of cardiac metastasis from cervical carcinoma is extremely rare. The diagnosis is made almost exclusively postmortem. There are few cases of premortem diagnosis, and it is believed that when cardiac metastasis are found in vivo, the prognosis is extremely poor. Due to the rarity of this conditio...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1006/gyno.1998.5009
更新日期：1998-06-01 00:00:00
abstract：BACKGROUND:Chemotherapy remains an essential part of the treatment of advanced ovarian cancer. Intraperitoneal (IP) administration has been demonstrated to provide a survival advantage over intravenous chemotherapy in three phase 3 studies. However, IP catheter complications have been a significant factor in aborting I...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2007.12.029
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVES:Endometriosis-associated ovarian carcinoma (EAOC) has recently received increasing attention due to its suggested biological behavior, distinctive from those of usual epithelial ovarian cancer. To elucidate some of the controversies on this intriguing entity, a series of patients with EAOC were compared to o...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.2001.6382
更新日期：2001-10-01 00:00:00
abstract：OBJECTIVE:Some authors have recommended the use of diagnostic laparoscopy as a pretreatment assessment step for conservative hormonal treatment in young women with endometrial cancer. The aim of this study was to determine the incidence of synchronous primary cancer of the endometrium and ovary in young women. METHODS...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2013.09.009
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVES:BRCA1 and BRCA2 germline mutations increase the risk of ovarian and breast cancer. Fallopian tube cancer has occasionally been observed in breast-ovarian cancer families. At our family cancer clinic we recently encountered two cases of Fallopian tube cancer within two families harboring a BRCA1 germline muta...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1999.5623
更新日期：2000-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to evaluate the impact of the 1999 national recommendations for ovarian cancer surgery in England to be performed by specialist surgeons in specialist centres. METHODS:A retrospective analysis of English cancer registry records, Hospital Episode Statistics (HES) data for all English ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2015.03.003
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of orally administered medroxyprogesterone acetate upon angiogenesis in the myometrium of patients with complex endometrial hyperplasia. METHODS:Microvessel counts in the myometrium of consecutive patients with complex endometrial hyperplasia, treated with oral medroxyprogesterone acet...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1998.5106
更新日期：1999-02-01 00:00:00
abstract：OBJECTIVE:To conduct a systematic review of the literature regarding the systemic treatment of advanced uterine sarcoma and provide an evidence-based summary of the available literature. METHODS:MEDLINE, EMBASE, and the Cochrane Library databases were searched. "Uterine sarcoma," "leiomyosarcoma," "mixed mesodermal tu...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2005.01.041
更新日期：2005-05-01 00:00:00
abstract：OBJECTIVE:While there is ample literature on prognostic factors for uterine cancer, currently there are nomeans to estimate an individual's risk for recurrence or to differentiate the risk of loco-regional recurrence from distant recurrence. We addressed this gap by developing nomograms to individualize the risk of rec...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.02.022
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVE:The objective of this study was to determine the efficacy of a low-dose oral granisetron plus intravenous dexamethasone prophylactic antiemetic regimen in patients receiving carboplatin-based chemotherapy. PATIENTS AND METHODS:Patients with gynecologic malignancies being treated with either single-agent carb...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1006/gyno.1998.5168
更新日期：1998-10-01 00:00:00
abstract：OBJECTIVE:The present study was undertaken to analyze the impact of epigenetic alterations with a main focus on nuclear area, aneuploidy, hyperploidy, and proliferation in 70 ovarian cancer specimens. METHODS:Morphometric changes and somatic chromosomal ploidy status were assessed by Feulgen spectrophotometry. DNA-hyp...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2010.12.332
更新日期：2011-04-01 00:00:00
abstract：OBJECTIVE:To evaluate the hospital-acquired condition (HAC) following oophorectomy and/or hysterectomy for gynecologic cancer patients based on clinical outcomes and costs. MATERIALS AND METHODS:Data were obtained from the Nationwide Inpatient Sample from 2005 to 2011. Chi-squared and Wilcoxon rank sum two-sample test...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.07.009
更新日期：2018-09-01 00:00:00
abstract：UNLABELLED:Surgical staging of apparent early stage adnexal carcinoma provides indispensable information. A significant number of patients are referred to tertiary centers with inadequate staging information. We report on our experience with late results of laparoscopic restaging procedure in uncompletely managed early...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.05.052
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVE:This study sought to develop and examine preliminary validity of the NCCN-FACT Ovarian Symptom Index-18 (NFOSI-18), a new ovarian cancer-specific symptom index comprised of symptoms rated as highest priority by both oncology clinical experts and women with advanced ovarian cancer. METHODS:Fifty-one women wit...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2010.09.025
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:To estimate the frequency of cervical cytologic/histologic discrepancies in a group of obstetric patients diagnosed as HIV infected by routine prenatal screening. Also, to determine if serum CD4 levels or sexually transmitted diseases (STDs) are associated with the occurrence of preinvasive cervical disease i...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1997.4683
更新日期：1997-06-01 00:00:00
abstract：:By serendipity we have had the opportunity to evaluate cis-platin-based chemotherapy in ovarian tumors of low malignant potential (LMP). Optimal (less than 1 cm residual disease) FIGO stage III ovarian carcinomas were randomly assigned to treatment with cisplatin plus cyclophosphamide with or without doxorubicin on a ...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0090-8258(91)90314-u
更新日期：1991-06-01 00:00:00
abstract：:To develop a mapping strategy for improved detection of p53 allelic loss associated with ovarian carcinoma, we utilized multiple intragenic polymorphisms and single-strand conformation polymorphism (SSCP) analysis. p53 allelotype distributions were defined for 80 ovarian cancer patients from germ-line DNA. All polymor...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1996.0122
更新日期：1996-05-01 00:00:00
