Faecal calprotectin and lactoferrin as markers of acute radiation proctitis: a pilot study of eight stool markers.

Abstract:

BACKGROUND:Non-invasive diagnostic tools to evaluate the severity of acute, radiation-induced proctitis are not readily available. The faecal excretion of eight markers of gut inflammation was therefore examined. Five proteins and three lipid derivates were analysed in sequential stool samples taken before and during radiation therapy. METHODS:Stool samples from 15 patients with prostate cancer scheduled for radiation therapy were examined. Pretreatment and in-treatment samples (2nd and 6th weeks) were measured by enzyme-linked immunosorbent assay (ELISA) (calprotectin, lactoferrin, transferrin, leukotriene B4, prostaglandin E2, thromboxane B2 and TNF alpha) or nephelometry (alpha 1-antitrypsin). RESULTS:Calprotectin and lactoferrin concentrations increased significantly during radiation treatment (P = 0.0005 and P = 0.019). Transferrin was detected in only 9 out of 45 samples. There were no changes in tumour necrosis factor alpha (TNF alpha), leukotriene B4, prostaglandin E2 and thromboxane B2 during treatment. alpha 1-antitrypsin could not be detected in any sample. CONCLUSIONS:This study indicates that faecal calprotectin and lactoferrin concentrations could be markers of acute, radiation-induced proctitis. Patient compliance and stability of the markers make this a promising method for clinical research. Eicosanoids could be measured in stool samples, but the concentrations did not increase with increasing radiation dose.

journal_name

Scand J Gastroenterol

authors

Larsen A,Hovdenak N,Karlsdottir A,Wentzel-Larsen T,Dahl O,Fagerhol MK

doi

10.1080/00365520410003614

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

1113-8

issue

11

eissn

0036-5521

issn

1502-7708

pii

TEXFF5YX6VK3X5BP

journal_volume

39

pub_type

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