REF2 encodes an RNA-binding protein directly involved in yeast mRNA 3'-end formation.

abstract：:We have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here we show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for...

journal_title：Molecular and cellular biology

authors： Burton ZF,Killeen M,Sopta M,Ortolan LG,Greenblatt J

更新日期：1988-04-01 00:00:00

abstract：:The stem-loop in the 5' untranslated region (UTR) of collagen α1(I) and α2(I) mRNAs (5'SL) is the key element regulating their stability and translation. Stabilization of collagen mRNAs is the predominant mechanism for high collagen expression in fibrosis. LARP6 binds the 5'SL of α1(I) and α2(I) mRNAs with high affini...

journal_title：Molecular and cellular biology

authors： Challa AA,Stefanovic B

更新日期：2011-09-01 00:00:00

abstract：:The tyrosine kinase activity of the epidermal growth factor (EGF) receptor is regulated by a truncated receptor of 100 kilodaltons (kDa) that contains the EGF-binding site but not the kinase domain. The inhibition of kinase is not due to competition for available EGF or for the kinase substrate-binding site. Chemical ...

journal_title：Molecular and cellular biology

authors： Basu A,Raghunath M,Bishayee S,Das M

更新日期：1989-02-01 00:00:00

abstract：:The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis...

journal_title：Molecular and cellular biology

authors： Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SM

更新日期：1986-12-01 00:00:00

abstract：:Genomic instability, including the ability to undergo gene amplification, is a hallmark of neoplastic cells. Similar to normal cells, "nonpermissive" REF52 cells do not develop resistance to N-(phosphonacetyl)-L-aspartate (PALA), an inhibitor of the synthesis of pyrimidine nucleotides, through amplification of cad, th...

journal_title：Molecular and cellular biology

authors： Chernova OB,Chernov MV,Ishizaka Y,Agarwal ML,Stark GR

更新日期：1998-01-01 00:00:00

abstract：:ZO-1, ZO-2, and ZO-3 are closely related MAGUK family proteins that localize at the cytoplasmic surface of tight junctions (TJs). ZO-1 and ZO-2 are expressed in both epithelia and endothelia, whereas ZO-3 is exclusively expressed in epithelia. In spite of intensive studies of these TJ MAGUKs, our knowledge of their fu...

journal_title：Molecular and cellular biology

authors： Adachi M,Inoko A,Hata M,Furuse K,Umeda K,Itoh M,Tsukita S

更新日期：2006-12-01 00:00:00

abstract：:Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1) signa...

journal_title：Molecular and cellular biology

authors： Llobet-Navas D,Rodriguez-Barrueco R,de la Iglesia-Vicente J,Olivan M,Castro V,Saucedo-Cuevas L,Marshall N,Putcha P,Castillo-Martin M,Bardot E,Ezhkova E,Iavarone A,Cordon-Cardo C,Silva JM

更新日期：2014-12-01 00:00:00

abstract：:Hydroxyurea (HU) is a DNA replication inhibitor that negatively affects both the elongation and initiation phases of replication and triggers the "intra-S phase checkpoint." Previous work with budding yeast has shown that, during a short exposure to HU, MEC1/RAD53 prevent initiation at some late S phase origins. In th...

journal_title：Molecular and cellular biology

authors： Alvino GM,Collingwood D,Murphy JM,Delrow J,Brewer BJ,Raghuraman MK

更新日期：2007-09-01 00:00:00

abstract：:The alpha-myosin heavy-chain (alpha-MHC) gene is the major structural protein in the adult rodent myocardium. Its expression is restricted to the heart by a complex interplay of trans-acting factors and their cis-acting sites. However, to date, the factors that have been shown to regulate expression of this gene have ...

journal_title：Molecular and cellular biology

authors： Molkentin JD,Kalvakolanu DV,Markham BE

更新日期：1994-07-01 00:00:00

abstract：:We have examined the ability of the simian virus 40 72-base pair enhancer segment to simultaneously activate multiple transcription units with plasmids that contain one, two, or three simian virus 40-based transcription units in various arrangements. After transfection into CV1 cells, the expression of a marker gene, ...

journal_title：Molecular and cellular biology

authors： Kadesch T,Berg P

更新日期：1986-07-01 00:00:00

abstract：:Two-dimensional protein gels were used to systematically assess changes in gene expression in Friend erythroleukemia cells after exposure to inducers of differentiation. A rapid decrease in expression of the stress protein HSP70 was observed after exposure to inducers. The kinetics of this change suggest that it may b...

journal_title：Molecular and cellular biology

authors： Hensold JO,Housman DE

更新日期：1988-05-01 00:00:00

abstract：:By using a retrovirus-derived vector system, we generated derivatives of the human colon cancer cell line HT29 that stably overexpress a full-length cDNA encoding the beta 1 isoform of rat protein kinase C (PKC). Two of these cell lines, PKC6 and PKC7, displayed an 11- to 15-fold increase in PKC activity when compared...

journal_title：Molecular and cellular biology

authors： Choi PM,Tchou-Wong KM,Weinstein IB

更新日期：1990-09-01 00:00:00

abstract：:Adipocytes constitute a major part of the bone marrow stroma in vivo and may play an active role in lymphohematopoiesis. Earlier studies had shown that the bone marrow stromal cell clone BMS2 was capable of adipocyte differentiation in vitro, in addition to its well-defined ability to support B lymphopoiesis. We now d...

journal_title：Molecular and cellular biology

authors： Gimble JM,Dorheim MA,Cheng Q,Pekala P,Enerback S,Ellingsworth L,Kincade PW,Wang CS

更新日期：1989-11-01 00:00:00

abstract：:Interferon A (IFN-A) genes are differentially expressed after virus induction. The differential expression of individual IFN-A genes is modulated by the specific transcription activators IFN regulatory factor 3 (IRF3) and IRF-7 and the homeoprotein transcription repressor Pitx1. We now show that repression by Pitx1 do...

journal_title：Molecular and cellular biology

authors： Island ML,Mesplede T,Darracq N,Bandu MT,Christeff N,Djian P,Drouin J,Navarro S

更新日期：2002-10-01 00:00:00

abstract：:The Drosophila dopa decarboxylase gene, Ddc, is expressed in the hypoderm and in a small number of cells in the central nervous system (CNS). The unique Ddc primary transcript is alternatively spliced in these two tissues. We investigated whether Ddc splicing in the CNS is a general property of the CNS or a unique pro...

journal_title：Molecular and cellular biology

authors： Shen J,Beall CJ,Hirsh J

更新日期：1993-08-01 00:00:00

abstract：:The steady-state mRNA levels of the proliferating cell nuclear antigen (PCNA) gene are growth regulated. In a previous paper (L. Ottavio, C.-D. Chang, M. G. Rizzo, S. Travali, C. Casadevall, and R. Baserga, Mol. Cell. Biol. 10:303-309, 1990), we reported that introns (especially intron 4) participate in growth regulat...

journal_title：Molecular and cellular biology

authors： Chang CD,Ottavio L,Travali S,Lipson KE,Baserga R

更新日期：1990-07-01 00:00:00

abstract：:Treatment of cells with tumor-promoting phorbol esters results in the activation but then depletion of phorbol ester-responsive protein kinase C (PKC) isoforms. The ubiquitin-proteasome pathway has been implicated in regulating the levels of many cellular proteins, including those involved in cell cycle control. We re...

journal_title：Molecular and cellular biology

authors： Lu Z,Liu D,Hornia A,Devonish W,Pagano M,Foster DA

更新日期：1998-02-01 00:00:00

abstract：:Autophosphorylated growth factor receptors provide binding sites for the src homology 2 domains of intracellular signaling molecules. In response to epidermal growth factor (EGF), the activated EGF receptor binds to a complex containing the signaling protein GRB2 and the Ras guanine nucleotide-releasing factor Sos, le...

journal_title：Molecular and cellular biology

authors： Li W,Nishimura R,Kashishian A,Batzer AG,Kim WJ,Cooper JA,Schlessinger J

更新日期：1994-01-01 00:00:00

abstract：:Nuclear microinjection of c-H-ras DNA induced DNA synthesis in reversibly nonproliferating quiescent human cells. The proto-oncogene and oncogene forms were equally effective inducers. In contrast, c-H-ras DNA either alone or in combination with the adenovirus E1A gene did not cause terminally nondividing senescent ce...

journal_title：Molecular and cellular biology

authors： Lumpkin CK,Knepper JE,Butel JS,Smith JR,Pereira-Smith OM

更新日期：1986-08-01 00:00:00

abstract：:The establishment of epigenetic marks, such as methylation on histone tails, is mechanistically linked to RNA polymerase II within active genes. To explore the interplay between these modifications in transcribed noncoding as well as coding sequences, we analyzed epigenetic modification and chromatin structure at high...

journal_title：Molecular and cellular biology

authors： Kim A,Kiefer CM,Dean A

更新日期：2007-02-01 00:00:00

abstract：:Nucleolar morphogenesis is a poorly defined process. Here we report that the Saccharomyces cerevisiae nucleolar trimethyl guanosine synthase I (Tgs1p), which specifically selects the m(7)G cap structure of snRNAs and snoRNAs for m(2,2,7)G conversion, is required not only for efficient pre-mRNA splicing but also for pr...

journal_title：Molecular and cellular biology

authors： Colau G,Thiry M,Leduc V,Bordonné R,Lafontaine DL

更新日期：2004-09-01 00:00:00

abstract：:Activation of PERK following the accumulation of unfolded proteins in the endoplasmic reticulum (ER) promotes translation inhibition and cell cycle arrest. PERK function is essential for cell survival following exposure of cells to ER stress, but the mechanisms whereby PERK signaling promotes cell survival are not tho...

journal_title：Molecular and cellular biology

authors： Cullinan SB,Zhang D,Hannink M,Arvisais E,Kaufman RJ,Diehl JA

更新日期：2003-10-01 00:00:00

abstract：:mof6-1 was originally isolated as a recessive mutation in Saccharomyces cerevisiae which promoted increased efficiencies of programmed -1 ribosomal frameshifting and rendered cells unable to maintain the killer virus. Here, we demonstrate that mof6-1 is a unique allele of the histone deacetylase RPD3, that the deacety...

journal_title：Molecular and cellular biology

authors： Meskauskas A,Baxter JL,Carr EA,Yasenchak J,Gallagher JE,Baserga SJ,Dinman JD

更新日期：2003-03-01 00:00:00

abstract：:Unlike other eukaryotes, the protein-coding genes of Trypanosoma cruzi are arranged in large polycistronic gene clusters transcribed by polymerase II (Pol II). Thus, it is thought that trypanosomes rely solely on posttranscriptional processes to regulate gene expression. Here, we show that the glucosylated thymine DNA...

journal_title：Molecular and cellular biology

authors： Ekanayake DK,Minning T,Weatherly B,Gunasekera K,Nilsson D,Tarleton R,Ochsenreiter T,Sabatini R

更新日期：2011-04-01 00:00:00

abstract：:Expression of the recombinant human U1-70K protein in COS cells resulted in its rapid transport to the nucleus, even when binding to U1 RNA was debilitated. Deletion analysis of the U1-70K protein revealed the existence of two segments of the protein which were independently capable of nuclear localization. One nuclea...

journal_title：Molecular and cellular biology

authors： Romac JM,Graff DH,Keene JD

更新日期：1994-07-01 00:00:00

abstract：:Tenomodulin (Tnmd) is a member of a new family of type II transmembrane glycoproteins. It is predominantly expressed in tendons, ligaments, and eyes, whereas the only other family member, chondromodulin I (ChM-I), is highly expressed in cartilage and at lower levels in the eye and thymus. The C-terminal extracellular ...

journal_title：Molecular and cellular biology

authors： Docheva D,Hunziker EB,Fässler R,Brandau O

更新日期：2005-01-01 00:00:00

abstract：:alpha 1-Antitrypsin (AT), the major elastase inhibitor in mammalian serum, is produced primarily in the liver. We have characterized AT gene structure and expression in the mouse species Mus caroli, which expresses high levels of AT in the kidneys as well as in the liver. Analysis of cDNA and genomic clones showed tha...

journal_title：Molecular and cellular biology

authors： Latimer JJ,Berger FG,Baumann H

更新日期：1990-02-01 00:00:00

abstract：:F9 embryonic teratocarcinoma stem cells differentiate into an epithelial cell type called extraembryonic endoderm when treated with retinoic acid (RA), a derivative of retinol (vitamin A). This differentiation is presumably mediated through the actions of retinoid receptors, the RARs and RXRs. To delineate the functio...

journal_title：Molecular and cellular biology

authors： Boylan JF,Lufkin T,Achkar CC,Taneja R,Chambon P,Gudas LJ

更新日期：1995-02-01 00:00:00

abstract：:The PH domain-containing proteins Slm1 and Slm2 were previously identified as effectors of the phosphatidylinositol-4,5-bisphosphate (PI4,5P(2)) and TORC2 signaling pathways. Here, we demonstrate that Slm1 and Slm2 are also targets of sphingolipid signaling during the heat shock response. We show that upon depletion o...

journal_title：Molecular and cellular biology

authors： Daquinag A,Fadri M,Jung SY,Qin J,Kunz J

更新日期：2007-01-01 00:00:00

abstract：:Transcription factor-induced reprogramming of somatic cells to pluripotency is mediated via profound alterations in the epigenetic landscape. The histone variant macroH2A1 (mH2A1) is a barrier to the cellular reprogramming process. We demonstrate here that mH2A1 blocks reprogramming and contributes to the preservation...

journal_title：Molecular and cellular biology

authors： Pliatska M,Kapasa M,Kokkalis A,Polyzos A,Thanos D

更新日期：2018-04-30 00:00:00