Abstract:
:C- kit encodes the membrane-bound tyrosine kinase KIT, whose expression has been identified in several types of human neoplasms. Recently, KIT has been reported to be a marker for chromophobe renal cell carcinoma (RCC) and renal angiomyolipoma. However, expression of this molecule has not been adequately studied in other renal tumors, particularly oncocytoma, which may morphologically resemble chromophobe RCC. In this study, we analyzed c- kit messenger RNA (mRNA) levels in 17 chromophobe RCCs and 20 renal oncocytomas obtained from complementary DNA (cDNA) microarrays. Furthermore, comprehensive immunohistochemical analysis of KIT protein using a monoclonal antibody was performed in 226 renal tumors including chromophobe RCC (n=40), oncocytoma (n=41), clear-cell RCC (n=40), renal angiomyolipoma (n=29), and papillary RCC (n=21) on tissue microarrays (TMAs) and was compared with immunostaining results from 25 chromophobe RCCs and 30 oncocytomas using standard sections. The staining intensity was semiquantitatively graded on a 3-tier scoring system. All chromophobe RCCs and oncocytomas showed significant overexpression of c- kit mRNA. The average increase of mRNA compared with normal kidney tissue was 7.4-fold for chromophobe RCCs and 7.4-fold for oncocytomas. Immunohistochemical expression of KIT was found in most chromophobe RCCs (95% in TMAs and 96% in conventional sections) and oncocytomas (88% in TMAs and 100% in conventional sections) but was infrequently observed in renal angiomyolipomas (17%), papillary RCCs (5%), and clear-cell RCCs (3%). Furthermore, the average KIT immunoreactivity in TMAs was stronger in chromophobe RCC (1.93) and oncocytoma (2.07) than in other subtypes of renal tumors tested, including angiomyolipomas (0.17), papillary RCCs (0.05), and clear-cell RCCs (0.03). In conclusion, we found a significant elevation of c- kit mRNA by cDNA expression microarrays and overexpression of KIT protein by immunohistochemistry not only in chromophobe RCCs but also in oncocytomas. In contrast, immunohistochemical expression of KIT was not detected in most other types of renal cell tumors evaluated. The differential expression of c- kit in these renal tumors may have diagnostic and therapeutic implications.
journal_name
Hum Patholjournal_title
Human pathologyauthors
Huo L,Sugimura J,Tretiakova MS,Patton KT,Gupta R,Popov B,Laskin WB,Yeldandi A,Teh BT,Yang XJdoi
10.1016/j.humpath.2005.01.011subject
Has Abstractpub_date
2005-03-01 00:00:00pages
262-8issue
3eissn
0046-8177issn
1532-8392pii
S0046817705000389journal_volume
36pub_type
杂志文章相关文献
HUMAN PATHOLOGY文献大全abstract::Pulmonary vein stenosis (PVS) is a rare disorder. Accurate diagnosis often requires anatomical examination. We report four children with pulmonary vein stenosis. Autopsy showed bilateral lesions in two patients who were thought clinically to have unilateral disease. A diagnosis of PVS was made at autopsy in the third ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(95)90011-x
更新日期:1995-08-01 00:00:00
abstract::The outcomes of patients with stage I non-small-cell lung cancer (NSCLC) vary greatly, with a 5-year survival rate of approximately 60%. This study evaluated a number of molecular markers that may aid in predicting prognosis in stage I NSCLC after surgical resection. Immunohistochemical (IHC) staining of p53, HER-2/ne...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2002.30183
更新日期:2002-01-01 00:00:00
abstract::Natural killer (NK)/T-cell lymphomas represent a rare type of lymphoma derived from either activated NK cells or, rarely, cytotoxic T cells. These lesions are most commonly extranodal and tend to present as destructive lesions within the midline facial structures. Other than the nasal cavity and paranasal sinuses, sev...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2006.01.032
更新日期:2006-06-01 00:00:00
abstract::Rosette-forming glioneuronal tumors of the fourth ventricle are rare brain tumors, and only 19 such lesions have been previously reported. This report presents the first case of a rosette-forming glioneuronal tumors arising from the spinal cord. A 44-year-old woman presented with a 15-year history of dissociated senso...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2008.11.010
更新日期:2009-06-01 00:00:00
abstract::Clinical stage I seminomas are effectively treated with surgery raising concerns as to when to give adjuvant radiation therapy given the risk of secondary malignancies. A recent randomized trial found tumor size and rete testis invasion to be the strongest predictors of relapse in clinical stage I seminomas. These 2 p...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2005.03.011
更新日期:2005-06-01 00:00:00
abstract::Basaloid carcinoma (BC) and large-cell neuroendocrine carcinoma (LCNEC) are 2 recently recognized variants of large-cell lung carcinomas that may overlap in their morphology, and are discriminated by expression of neuroendocrine markers in LCNEC. Because thyroid transcription factor 1 (TTF-1) is expressed in lung aden...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2001.27110
更新日期:2001-09-01 00:00:00
abstract::Abnormalities of the cell cycle are present in all neoplasms. Cyclin E, which regulates the G1/S phase transition of the cell cycle, plays an important role in many different cancers. To further investigate the role of cyclin E in invasive breast ductal carcinomas in South African women, representative sections from 1...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2001.21141
更新日期:2001-01-01 00:00:00
abstract::The natural history of pediatric nonalcoholic steatohepatitis is still unknown; however, there are differences between adult and pediatric presentation. Apoptosis may play an important role in pathophysiologic pathways involved in liver damage and progression. Our aim was to detect early apoptosis markers, activated c...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2008.04.022
更新日期:2008-12-01 00:00:00
abstract::AT-rich interactive domain 1A (ARID1A) is tumor suppressor gene that interacts with BRG1 adenosine triphosphatase to form a SWI/SNF chromatin remodeling protein complex. Inactivation of ARID1A has been described in several neoplasms, including epithelial ovarian and endometrial carcinomas, and has been correlated with...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2014.07.003
更新日期:2014-11-01 00:00:00
abstract::Most esophageal intraepithelial lymphocytes (IELs) express T-cell markers. Increased numbers of esophageal IELs have been shown in reflux esophagitis. The cytotoxic potential and activity of esophageal IELs have not as yet been examined. Our objectives were to determine whether esophageal IELs express the recently des...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(99)90114-4
更新日期:1999-04-01 00:00:00
abstract::Altered expression of oncogenes and tumor suppressor genes, the normal function of which is to regulate cell growth and differentiation, represents a central event in the pathogenesis of human cancer. Aberrant expression of these genes is often a result of a mutational event. In vitro amplification of DNA with the pol...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/0046-8177(94)90220-8
更新日期:1994-06-01 00:00:00
abstract::Acinar cell carcinoma (ACC) is a rare tumor that differentiates toward pancreatic acinar cells and shows evidence of pancreatic enzyme production. Mixed acinar-neuroendocrine carcinoma (MANC) is defined as having more than 30% of both acinar and neuroendocrine cell types as per immunohistochemistry analysis. Trypsin i...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2020.07.019
更新日期:2020-09-01 00:00:00
abstract::Heterotopic pancreatic parenchyma recapitulates the normal pancreas in extrapancreatic locations and, on rare occasions, can even give rise to pancreatic adenocarcinoma. The genetic signatures of pancreatic adenocarcinoma and its precursor lesions are well characterized. We explored the genetic alterations in precurso...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2015.09.023
更新日期:2016-02-01 00:00:00
abstract::In the present study the clinical and pathological evolution of a reactive-like B-cell lymphoproliferative disorder with an unusually high content of T cells is described. Immunogenotypic analysis showed that the same phenotypically atypical B-cell clone, characterized by the unusual presence of an immunoglobulin (Ig)...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(95)90070-5
更新日期:1995-03-01 00:00:00
abstract::The histologic findings in lymph nodes were used to identify eight patients, who are not in a high-risk group, with human immunodeficiency virus (HIV) infection. In order to determine the specificity of these findings, the histologic and clinical findings in these patients were compared with the histologic and clinica...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(88)80269-7
更新日期:1988-07-01 00:00:00
abstract::Columnar cell lesions of the breast are a spectrum of lesions in the terminal duct lobular units, which include columnar cell change, columnar cell hyperplasia, and columnar cell change or columnar cell hyperplasia with atypia. The latter was designated by the World Health Organization as flat epithelial atypia. We st...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2009.12.003
更新日期:2010-06-01 00:00:00
abstract::High cathepsin D (cath-D) concentration in breast cancer cytosol is associated with increased risk of metastasis. To specify the relative contribution of the different cells types responsible for cath-D level in cytosol, we validated semiquantitative cath-D immunoperoxidase staining on formalin-fixed, paraffin-embedde...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(94)90004-3
更新日期:1994-09-01 00:00:00
abstract::Fibrillar proteins with a role in cellular shape and motility are present in the cytoplasm of most animal cells. They vary greatly in size, organization, and reactivity according to cell type and can be separately identified by the use of recently developed monospecific antisera and indirect immunofluorescence stainin...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(81)80026-3
更新日期:1981-05-01 00:00:00
abstract::Myocardial apoptosis has previously been observed in human acute myocardial infarcts. We examined the time of appearance and extent of apoptosis in human acute myocardial infarcts, and compared these with necrotic cell death. Because nuclear internucleosomal DNA fragmentation is a hallmark of apoptosis, autopsied tiss...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(97)90039-3
更新日期:1997-04-01 00:00:00
abstract::Yes-associated protein, a downstream effector of the Hippo signaling pathway, has been linked to progression of non-small-cell lung carcinoma. The aim of this study was to investigate expression of Yes-associated protein in lung adenocarcinoma and squamous cell carcinoma. Associations of Yes-associated protein express...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2010.08.003
更新日期:2011-03-01 00:00:00
abstract::A primary neoplasm of the proximal humerus in a 68-year-old woman was unique histologically in that it contained both malignant cartilaginous and squamous cell components. The epithelial differentiation was confirmed by the demonstration of keratin by immunohistochemical techniques and of basement membrane, tonofilame...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(83)80227-5
更新日期:1986-03-01 00:00:00
abstract::The previously unreported association of granulomatous gastritis and mononeuritis multiplex occurring in the setting of a vasculitic syndrome is described. The two conditions are considered to be associated and to be immune mediated. The previously accepted concept of isolated granulomatous gastritis is disputed. ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(91)90018-k
更新日期:1991-10-01 00:00:00
abstract::We describe a method for the immunocytochemical assessment of estrogen receptor (ER) status on routinely processed, formalin-fixed tissue using a recently developed commercially available monoclonal antibody (Dako 1D5) with a microwave antigen retrieval technique. A series of 90 cases of human breast carcinoma was ana...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(95)90060-8
更新日期:1995-03-01 00:00:00
abstract::Current regulations require that departments of pathology have a structured and active program of quality assurance (QA) and quality improvement (QI), with the goals of enhancing patient safety, minimizing error, ensuring timely delivery of reports, and monitoring physician competence. Types of potential error may evo...
journal_title:Human pathology
pub_type: 指南,杂志文章
doi:10.1016/j.humpath.2006.03.010
更新日期:2006-08-01 00:00:00
abstract::Neuronal intranuclear inclusion disease (NIID) is a progressive, usually fatal degenerative neurologic condition characterized by eosinophilic, intranuclear inclusions in neurons of the central and peripheral nervous system. We report a boy with onset of disease manifestations at age 3 and death at age 9, who showed c...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(91)90296-2
更新日期:1991-07-01 00:00:00
abstract::The mucin profile of 24 endoscopic biopsies of heterotopic gastric mucosa (HGM) of the upper esophagus in adults and a control group of ten cases of Meckel's diverticula containing heterotopic gastric mucosa were studied with two combined histochemical methods: alcian blue pH 2.5/PAS and high iron diamine/alcian blue ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(88)80285-5
更新日期:1988-11-01 00:00:00
abstract::Follicular dendritic cells (FDCs) are a specialized type of antigen-presenting dendritic cells that are largely restricted to lymphoid follicles. They form dense three-dimensional meshwork patterns within benign follicles, which maintain the follicular architecture. The FDC function is to bind and retain antigens by l...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/j.humpath.2012.10.005
更新日期:2013-06-01 00:00:00
abstract::Hydrophilic polymers are commonly applied as surface coatings on vascular devices and have been shown to dissociate during endovascular use, causing hydrophilic polymer embolism (HPE). Adverse effects related to this phenomenon have been recognized and reported. The prevalence of this complication is unknown. We condu...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2014.09.011
更新日期:2015-02-01 00:00:00
abstract::Primary mediastinal seminomas are unusual tumors that can present in a pure form or as part of a mixed germ cell tumor. Contrary to testicular seminomas, little is known about the expression of novel immunohistochemical markers in mediastinal seminomas. This study investigates the immunohistochemical features of these...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2014.11.009
更新日期:2015-03-01 00:00:00
abstract::We developed a procedure based on computerized image analysis to establish objective criteria for the differential diagnosis between mesothelial hyperplasia and cancer in peritoneal tissue samples obtained at second-look operations for ovarian cancer. The tumor tissue after chemotherapy was classified as "nonresponsiv...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(93)90292-o
更新日期:1993-02-01 00:00:00