Preparation of the N-(4'-hydroxy-[3'-125I]iodophenethyl)-6-(4-O-diethylstilbestryl)hexanam ide for diethylstilbestrol radioimmunoassays.

abstract：:Contrary to the long-held postulate of steroid-hormone binding globulin action, these protein carriers of steroids are major players in steroid actions in the body. This manuscript will focus on our work with sex hormone binding globulin (SHBG) and corticosteroid binding globulin (CBG) and demonstrate how they are act...

journal_title：Steroids

authors： Caldwell JD,Jirikowski GF

更新日期：2014-03-01 00:00:00

abstract：:The synthesis of (22E,24R)-5 alpha-ergosta-2,22-dien-6-one (2), a key intermediate to the preparation of 24-epibrassinolide (1), was achieved through the 3,6-diketone 8 in a 40% overall yield in five steps starting from ergosterol (3). ...

journal_title：Steroids

authors： Brosa C,Puig R,Comas X,Fernández C

更新日期：1996-09-01 00:00:00

abstract：:The properties of 5-ene-3 beta hydroxysteroid oxidoreductase (3 beta-HSD) from human placental homogenates were studied in vitro. The apparent Michaelis constants for 3 beta-HSD with the substrates pregnenolone (delta 5P) and dehydroepiandrosterone (DHA) were 170 nM and nM respectively. The optimal pH for both these s...

journal_title：Steroids

authors： Yoshida N

更新日期：1979-01-01 00:00:00

abstract：:The steroid receptor-positive human ovarian cancer (BG-1) was evaluated to determine its usefulness as a tumor model. This tumor grows in intact male and female nude mice without hormone supplements. Moreover, its growth was significantly accelerated in ovariectomized mice, and the increased growth rate could be rever...

journal_title：Steroids

authors： Zimniski SJ,Garola RE,Fendl K,Peterson CM

更新日期：1989-12-01 00:00:00

abstract：:This paper describes three simple and short methods for the conversion of cholic acid into cholylaldehyde with protected hydroxyl groups. The first method involves lithium aluminum hydride reduction of the tetrahydropyranyl ether of methyl cholate and oxidation of the resulting primary alcohol with pyridinium chloroch...

journal_title：Steroids

authors： Batta AK,Tint GS,Dayal B,Shefer S,Salen G

更新日期：1982-06-01 00:00:00

abstract：:Postmitochondrial supernatant (PMS) (1) has been prepared from the homogenate of rat seminal vesicles and the characteristics of the binding reaction of 5alpha-dihydrotestosterone (DHT) to the cytoplasmic androgen receptor have been studied using a charcoal adsorption procedure. At 0 degrees C apparent equilibrium of ...

journal_title：Steroids

authors： Zakár T,Tóth M

更新日期：1977-12-01 00:00:00

abstract：:The purpose of this study was to investigate the influence of plasma steroid-binding proteins on androgen metabolism in intact leukocytes prepared from normal male and female blood samples. Leukocyte preparations were incubated for 24 h at 37 degrees C with either labeled or unlabeled testosterone (T), 5 alpha-dihydro...

journal_title：Steroids

authors： Déchaud H,Goujon R,Claustrat F,Boucherat M,Pugeat M

更新日期：1995-02-01 00:00:00

abstract：:The steroid nucleus is an interesting scaffold for the development of new therapeutic agents. Within the goal of identifying anticancer agents, new pregnane derivatives were prepared by using a sequence of liquid and solid-phase reactions. After we dehydrated epi-allopregnanolone in one step with diethylaminosulfur tr...

journal_title：Steroids

authors： Jegham H,Maltais R,Dufour P,Roy J,Poirier D

更新日期：2012-11-01 00:00:00

abstract：:A radioimmunoassay of three deoxycorticoids, namely 11 beta,17 alpha-dihydroxy-4-pregnene-3,20-dione (21-deoxycortisol), 17 alpha,21-dihydroxy-4-pregnene-3,20-dione (11-deoxycortisol), and 21-hydroxy-4-pregnene-3,20-dione (11-deoxycorticosterone) which are important for differential diagnosis of congenital adrenal dis...

journal_title：Steroids

authors： Hill M,Lapcík O,Hampl R,Stárka L,Putz Z

更新日期：1995-09-01 00:00:00

abstract：:Human placental estradiol 17 beta-dehydrogenase (E.C. 1.1.1.62) was inactivated at pH 6.3 by 3-bromo [2'-14C] acetoxy-1,3,5(10) estratrien-17-one, a know substrate. The affinity-alkylated enzyme was then hydrolyzed by trypsin. Radioactive peptides were initially isolated by gel filtration and identified according to w...

journal_title：Steroids

authors： Murdock GL,Warren JC

更新日期：1982-02-01 00:00:00

abstract：:Preparative chemical methods for the synthesis of eight oxidative transformation products of ethinyl estradiol (EE) and norethindrone acetate (NA) are described. The prepared materials are useful as reference materials and standards for pharmaceutical analysis of EE and NA as bulk chemical or in formulated product. Al...

journal_title：Steroids

authors： Ekhato IV,Hurley T,Lovdahl M,Revitte TJ,Guo L,Huang Y,Clipper S,Colson C

更新日期：2002-03-01 00:00:00

abstract：:The catalytic cycloalumination of 2'-methylidene-2',3'-ethano-(5α)-cholestane with Et3Al catalyzed by Cp2ZrCl2 was performed for the first time to give spiro[2',3'-ethano-(5α)-cholestane-2',3″-aluminacyclopentane] in a ~75% yield and with high stereoselectivity (>98%). The obtained cyclic organoaluminum compound was t...

journal_title：Steroids

authors： D'yakonov VA,Tuktarova RA,Islamov II,Khalilov LM,Dzhemilev UM

更新日期：2013-12-11 00:00:00

abstract：:Fourteen esters (formate, acetate, propionate, butyrate, hexanoate, heptanoate, and benzoate) located at C-11 of 11 beta-hydroxyesterone and 11 beta-hydroxyestradiol-17 beta were synthesized and evaluated for uterotropic and gonadotropin release inhibition in rats, as well as their ability to displace (3H) estradiol-1...

journal_title：Steroids

authors： Segaloff A,Gabbard RB

更新日期：1984-01-01 00:00:00

abstract：:7 beta-Methyl-chenodeoxycholic acid (7-MeCDC, 3 alpha, 7 alpha-dihydroxy-7 beta-methyl-5 beta-cholan-24-oic acid), 7 alpha-methyl-ursodeoxycholic acid (7-MeUDC, 3 alpha, 7 beta-dihydroxy-7 alpha-methyl-5 beta-cholan-24-oic acid), 7 xi-methyl-lithocholic acid (7-MeLC, 3 alpha-hydroxy-7 xi-methyl-5 beta-cholan-24-oic ac...

journal_title：Steroids

authors： Hylemon PB,Moody DP,Cohen BI,Une M,Mosbach EH

更新日期：1984-10-01 00:00:00

abstract：:The leaky heme mutants G204, G216, and G214 are shown to accumulate exogenous sterols. Unlike hem mutants which have complete blocks in the heme pathway, these strains do not require ergosterol, methionine, or unsaturated fatty acids for growth. The addition of aminolevulinic acid to the growth medium inhibited sterol...

journal_title：Steroids

authors： Shinabarger DL,Keesler GA,Parks LW

更新日期：1989-03-01 00:00:00

abstract：:A mechanistic study on the nucleophilic substitution of a strictly geometric 21-bromo-3alpha-hydroxyl-3beta-methoxymethyl-5alpha-pregnan-20-one was described. Reaction of the alpha-bromoketone with excess lithium imidazole followed by the addition of extra bases including n-butyllithium, methyllithium, lithium piperid...

journal_title：Steroids

authors： Chen CY,Wong FF,Lee YH,Chou SY,Huang JJ,Yeh MY

更新日期：2006-11-01 00:00:00

abstract：:We assayed Type 1 (high K+) and Type 2 (high Na+) human breast cyst fluids for DHEA-S. When an antibody specific for the 3-sulfoconjugate end of DHEA-S was used, Type 1 cyst fluids (n = 18) showed a content of 114 +/- 68 micrograms/mL (mean +/- sigma) and Type 2 cyst fluids (n = 14) of 35 +/- 17 micrograms/mL (P less ...

journal_title：Steroids

authors： Chasalow FI,Blethen SL,Bradlow HL

更新日期：1988-09-01 00:00:00

abstract：:As demonstrated for pregnenolone, saturated ketones are conveniently alpha-hydroxymethylated by their transformation into a lithium enolate and by the reaction of the latter with formaldehydr. The 17-hydroxymethylpregnenolone prepared by this method in very good yield was readily converted to 17-hydroxymethylprogester...

journal_title：Steroids

authors： Mukherjee D,Engel CR

更新日期：1979-11-01 00:00:00

abstract：:Following i.v. administration of [4-14C]cortisol, various sulfate conjugated metabolites of cortisol in urine were identified and their respective excretion rates measured. The results obtained demonstrated the following: 1) sulfate conjugates as a group are excreted considerably slower than glucuronide conjugates; 2)...

journal_title：Steroids

authors： Kornel L,Miyabo S

更新日期：1975-06-01 00:00:00

abstract：:The synthesis of the esters of norethisterone (17 alpha-ethynyl-17 beta-hydroxyestr-4-en-3-one) with three bile acids and of the cholesteryl carbonate of norethisterone are described. :This article reports the synthesis of the esters of norethisterone (17 alpha-ethynl-17beta-hydroxyestr-4-en-3-one) with 3 bile acids ...

journal_title：Steroids

authors： Herz JE,Sandoval J

更新日期：1983-03-01 00:00:00

abstract：:By analyzing the steroidal content of fresh whole plants of Tacca subflabellata (Taccaceae), we isolated one sapogenin and eight glycosides with four kinds of steroidal skeletons including four new glycosides, named taccasubosides A-D (1-4), together with five known compounds. Among them, compound 1 is the first penta...

journal_title：Steroids

authors： Li L,Ni W,Li XR,Hua Y,Fang PL,Kong LM,Pan LL,Li Y,Chen CX,Liu HY

更新日期：2011-09-01 00:00:00

abstract：:It has been previously demonstrated that 1,25 dihydroxyvitamin D(3) (1,25-D(3)) exerts inhibitory effects in breast cancer cells. The aim of this study was to determine whether mitogen-activated protein kinase (MAPK) pathways are associated with 1,25-D(3)-induced cell death in breast cancer. We used three breast cell ...

journal_title：Steroids

authors： Brosseau CM,Pirianov G,Colston KW

更新日期：2010-12-12 00:00:00

abstract：:Cell cycle regulator p21(CIP1) has controversial biological effects in breast cancer since in spite of its role as cell cycle inhibitor and promoter of cellular senescence, it also induces cell proliferation and chemoteraphy resistance. We here explored the molecular mechanisms involved in progestin regulation of p21(...

journal_title：Steroids

authors： Diaz Flaqué MC,Vicario R,Proietti CJ,Izzo F,Schillaci R,Elizalde PV

更新日期：2013-06-01 00:00:00

abstract：:Data in animal models and surveys in humans have revealed psychiatric complications of long-term anabolic androgenic steroid abuse. However, the neurobiochemical mechanisms behind the observed behavioral changes are poorly understood. The aim of the present study was to investigate the effects of nandrolone decanoate ...

journal_title：Steroids

authors： Zotti M,Tucci P,Colaianna M,Morgese MG,Mhillaj E,Schiavone S,Scaccianoce S,Cuomo V,Trabace L

更新日期：2014-01-01 00:00:00

abstract：:During the periparturient period, dairy cows are subjected to physiological changes that may induce immunosuppression and an increased susceptibility of the animal to bacterial infections such as mastitis. The incidence of clinical environmental mastitis is high during the last period of gestation, at parturition and ...

journal_title：Steroids

authors： Winters KR,Meyer E,Van Merris VM,Van Den Broeck WL,Duchateau L,Burvenich C

更新日期：2003-05-01 00:00:00

abstract：:Two unnatural steroid sapogenins bearing a furospirostane side chain were prepared starting from the readily available spirostane sapogenins, tigogenin and diosgenin following a synthetic protocol that included: (i) introduction of a carbonyl group at position C-23, (ii) diacetoxyiodobenzene-induced F-ring contraction...

journal_title：Steroids

authors： Sánchez-Flores J,Romero-Ávila M,Rosado-Abón A,Flores-Álamo M,Iglesias-Arteaga MA

更新日期：2013-09-01 00:00:00

abstract：:The effects of progesterone, its agonists (progestin RU-5020, glucocorticoid RU-26988) and antagonist (antiprogesterone, anti-glucocorticoid RU-486) were tested on isolated fat cells in vitro. When added to the incubation medium, all four steroids decreased basal glucose oxidation. The inhibitory effect of the steroid...

journal_title：Steroids

authors： Sutter-Dub MT,Kaaya A,Sfaxi Al,Sodoyez-Goffaux F,Sodoyez JC,Sutter BC

更新日期：1988-11-01 00:00:00

abstract：:Significant changes in % free serum E2, T and P were observed during the annual cycle of the turtle. In contrast no significant changes in SHBG affinity constants (2.29-4.48 x 10(8) M-7) were found and binding capacity for estradiol 17B was between 3.47 and 3.96 x 10(-7) M except in preovulatory animals (2.00 +/- 0.25...

journal_title：Steroids

authors： Albert C,Callard IP

更新日期：1988-10-01 00:00:00

abstract：:A highly active inhibitor of the binding of estrone and estradiol-17beta to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compou...

journal_title：Steroids

authors： Benassayag C,Vallette G,Delorme J,Savu L,Nunez EA,Jayle MF

更新日期：1977-12-01 00:00:00

abstract：:The ring A monoglucuronides and monosulfates of 2-hydroxyestriol were synthesized from 2-hydroxyestriol 16,17-diacetate by means of the Koenigs-Knorr reaction with methyl alpha-acetobromoglucuronate and sulfation with sulfur trioxide-pyridine complex, respectively. The conjugated positions of these compounds were defi...

journal_title：Steroids

authors： Ohkubo T,Wakasawa T,Nambara T

更新日期：1990-03-01 00:00:00