Abstract：
BACKGROUND:Gonadal differentiation in the mammalian fetus involves a complex dose-dependent genetic network. Initiation and progression of fetal ovarian and testicular pathways are accompanied by dynamic expression patterns of thousands of genes. We postulate these expression patterns are regulated by small non-coding RNAs called microRNAs (miRNAs). The aim of this study was to identify the expression of miRNAs in mammalian fetal gonads using sheep as a model. METHODS:We determined the expression of 128 miRNAs by real time PCR in early-gestational (gestational day (GD) 42) and mid-gestational (GD75) sheep ovaries and testes. Expression data were further examined and validated by bioinformatic analysis. RESULTS:Expression analysis revealed significant differences between ovaries and testes among 24 miRNAs at GD42, and 43 miRNAs at GD75. Bioinformatic analysis revealed that a number of differentially expressed miRNAs are predicted to target genes known to be important in mammalian gonadal development, including ESR1, CYP19A1, and SOX9. In situ hybridization revealed miR-22 localization within fetal testicular cords. As estrogen signaling is important in human and sheep ovarian development, these data indicate that miR-22 is involved in repressing estrogen signaling within fetal testes. CONCLUSIONS:Based on our results we postulate that gene expression networks underlying fetal gonadal development are regulated by miRNAs.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Torley KJ,da Silveira JC,Smith P,Anthony RV,Veeramachaneni DN,Winger QA,Bouma GJdoi
10.1186/1477-7827-9-2subject
Has Abstractpub_date
2011-01-11 00:00:00pages
2issn
1477-7827pii
1477-7827-9-2journal_volume
9pub_type
杂志文章abstract：BACKGROUND:The cell membrane water channel protein, aquaporins (AQPs), regulate cellular water transport and cell volume and play a key role in water homeostasis. Recently, AQPs are considered as important players in the field of reproduction. In previous studies, we have established the presence of AQP1 and 5 in porci...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0004-5
更新日期：2015-02-18 00:00:00
abstract：BACKGROUND:17alpha-Estradiol has been considered as the hormonally inactive isomer of 17beta-estradiol. Recently, nongenomic (smooth muscle relaxation) and genomic (light estrogenic activity) effects of 17alpha-estradiol have been reported, but no reports have yet determined its possible antiestrogenic activity. Theref...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-3-30
更新日期：2005-07-21 00:00:00
abstract：:The aim of this study was to analyse the multigenerational effects of para-nonylphenol (NP) and resveratrol (RES) on the body weight, organ weight and reproductive fitness of outbred CD-1 mice. The data indicate that in male mice, NP had an effect on the weight of selected reproductive organs and the kidneys in the pa...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-1-30
更新日期：2003-03-24 00:00:00
abstract：BACKGROUND:Decidualization is a prerequisite for successful implantation and the establishment of pregnancy. A critical role of impaired decidualization in subfertility has been established. In human endometrial stromal cells (hESCs), Krüppel-like factor 12 (KLF12) and Nur77 are novel regulators of decidualization. We ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-017-0243-8
更新日期：2017-03-31 00:00:00
abstract：BACKGROUND:Macrophage migration inhibitory factor (MIF) has special pro-inflammatory roles, affecting the functions of macrophages and lymphocytes and counter-regulating the effects of glucocorticoids on the immune response. The conspicuous expression of MIF during human implantation and early embryonic development als...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-8-95
更新日期：2010-08-04 00:00:00
abstract：:The present study evaluates the participation of the vagus nerve in pre-pubertal rats with unilateral ovariectomy on puberty onset, and on progesterone, testosterone and estradiol serum levels, and the compensatory responses of the ovary. Unilateral vagotomy did not modify the onset of puberty in unilaterally ovariect...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-5-24
更新日期：2007-06-13 00:00:00
abstract：OBJECTIVES:To report the occurrence of a rare case of a huge benign ovarian tumour (mucinous cystadenoma) in Jazan city, Saudi Arabia. PATIENTS:Our reported case was a middle-aged Saudi woman presented with marked abdominal distension and discomfort at the gynaecology clinic of Jazan General Hospital, Jazan city, Saud...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-8-24
更新日期：2010-03-11 00:00:00
abstract：:LINE1 retrotransposons are mobile DNA elements that copy and paste themselves into new sites in the genome. To ensure their evolutionary success, heritable new LINE-1 insertions accumulate in cells that can transmit genetic information to the next generation (i.e., germ cells and embryonic stem cells). It is our hypot...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/s12958-020-0564-x
更新日期：2020-01-21 00:00:00
abstract：BACKGROUND:This study was designed to determine whether small diameter needles for oocyte retrieval alter oocyte yields in patients undergoing IVF in comparison to standard large diameter needles. METHODS:We conducted a prospective pilot study of 21 consecutive favorable prognosis patients. In each patient one ovary w...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/1477-7827-11-22
更新日期：2013-03-20 00:00:00
abstract：BACKGROUND:With infertility populations rapidly aging, treatments improving pregnancy chances assume increasing clinical importance. Dehydroepiandrosterone (DHEA) has been reported to improve pregnancy rates and lower miscarriage rates in women with diminished ovarian function. This study was planned to evaluate whethe...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12958-015-0014-3
更新日期：2015-03-09 00:00:00
abstract：:Sexual health is strictly related with general health in both genders. In presence of a sexual dysfunction, the expert in sexual medicine aims to discover the specific weight of the physical and psychological factors can cause or con-cause the sexual problem. At the same time, a sexual dysfunction can represent a mark...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/s12958-019-0557-9
更新日期：2020-02-17 00:00:00
abstract：:Although much is known about the reproductive biology of pond-breeding frogs, there is comparatively little information about terrestrial-breeding anurans, a highly successful and diverse group. This study investigates the activation and in vitro fertilization of eggs of the Puerto Rican coqui frog obtained by hormona...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-2-60
更新日期：2004-08-05 00:00:00
abstract：BACKGROUND:Meta-analysis revealed that embryo placement 20 mm from the fundal endometrial surface resulted in higher pregnancy rate, ongoing pregnancy rate, and live birth rate compared with placement 10 mm from the fundal endometrial surface. Pregnancy and implantation rates according to relative position were higher ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12958-015-0072-6
更新日期：2015-07-29 00:00:00
abstract：BACKGROUND:The aim of this randomized controlled trial was to compare the efficacy of extended letrozole regimen with clomiphene citrate in women with unexplained infertility undergoing superovulation and intrauterine insemination (IUI). METHODS:Two hundred and fourteen patients with unexplained infertility were rando...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1186/1477-7827-9-84
更新日期：2011-06-21 00:00:00
abstract：:Cloning mammals by nuclear transfer is a powerful technique that is quickly advancing the development of genetically defined animal models. However, the overall efficiency of nuclear transfer is still very low and several hurdles remain before the power of this technique will be fully harnessed. Among these hurdles in...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-1-84
更新日期：2003-11-07 00:00:00
abstract：:Fertilin alpha (ADAM-1) and beta (ADAM-2) are integral membrane proteins of the ADAM family that form a fertilin complex involved in key steps of the sperm-oocyte membrane interaction. In the present work, we analyzed the presence of ADAM-1 and ADAM-2 mRNAs, the spermatozoa proteins' processing and their sub-cellular ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-9-96
更新日期：2011-06-30 00:00:00
abstract：BACKGROUND:The transformation of endometrium into decidua is essential for normal implantation of the blastocyst. However, the post-transcriptional regulation and the miRNAs involved in decidualization remain poorly understood. Here, we examined microRNA-181a (miR-181a) expression in decidualized human endometrial stro...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0019-y
更新日期：2015-03-26 00:00:00
abstract：BACKGROUND:Low functional ovarian reserve (LFOR) has been associated with hypoandrogenemia and increased embryo aneuploidy, while androgen supplementation has been reported to improve aneuploidy rates. We, therefore, assessed whether in infertile women undergoing in vitro fertilization (IVF) androgen concentrations are...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0034-z
更新日期：2015-05-06 00:00:00
abstract：BACKGROUND:The physiological state of the dominant follicle is important as it may be linked to the competence of the oocyte within. The objective of this study was to analyze, by transcriptomic analysis, the changes occurring in granulosa cells from dominant follicles at different phases of follicular growth. METHODS...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0010-7
更新日期：2015-03-08 00:00:00
abstract：BACKGROUND:Dehydroepinadrosterone (DHEA) supplementation improves pregnancy chances in women with diminished ovarian reserve (DOR), by possibly reducing aneuploidy. Since a large majority of spontaneous miscarriages are associated with aneuploidy, one can speculate that DHEA supplementation may also reduce miscarriage ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-108
更新日期：2009-10-07 00:00:00
abstract：BACKGROUND:Flow cytometric sorting can be used to separate sperm based on sex chromosome content. Differential fluorescence emitted by stained X- vs. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X- or Y-bearing sperm for use to influence the likelihood that the offspring will ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 临床试验,杂志文章
doi：10.1186/1477-7827-12-106
更新日期：2014-11-24 00:00:00
abstract：BACKGROUND:We characterized the spectrum and etiology of hypogonadism in a cohort of Prader-Willi syndrome (PWS) adolescents and adults. METHODS:Reproductive hormonal profiles and physical examination were performed on 19 males and 16 females ages 16-34 years with PWS. Gonadotropins, sex-steroids, inhibin B (INB) and ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-39
更新日期：2012-05-06 00:00:00
abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) has been recognized as a metabolic disorder, manifested by abdominal obesity, insulin resistance, dyslipidemia and hypertension. Pigment epithelium-derived factor (PEDF), a member of the serine protease inhibitor family, is a pleiotropic protein known for its antiangiogenic, ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-77
更新日期：2013-08-14 00:00:00
abstract：BACKGROUND:During the estrous cycle, the rat uterine endometrium undergoes many changes such as cell proliferation and apoptosis. If implantation occurs, stromal cells differentiate into decidual cells and near the end of pregnancy, a second wave of apoptosis occurs. This process called decidual regression, is tightly ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-80
更新日期：2009-08-05 00:00:00
abstract：:Recent reports indicate that functional mouse oocytes and sperm can be derived in vitro from somatic cell lines. We hypothesize that in adult human ovaries, mesenchymal cells in the tunica albuginea (TA) are bipotent progenitors with a commitment for both primitive granulosa and germ cells. We investigated ovaries of ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-2-20
更新日期：2004-04-28 00:00:00
abstract：:A recent reassessment of the phylogenetic affinities of cetaceans makes it timely to compare their placentation with that of the artiodactyls. We studied the placentae of two sympatric species of dolphin from the Amazon River Basin, representing two distinct families. The umbilical cord branched to supply a bilobed al...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-5-26
更新日期：2007-06-28 00:00:00
abstract：:The aim of our research was to examine the ability of density gradient preparation BoviPure and swim up method on bull sperm separation and in vitro embryo production (IVP) systems. Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concentration, membrane activ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-4-58
更新日期：2006-11-13 00:00:00
abstract：BACKGROUND:Considering that clinical trial studies are limited in polycystic ovary syndrome (PCOS) patients, and there is no consensus on an optimum endometrial preparation protocol for frozen embryo transfer (FET), the present study was designed as a randomized clinical trial to compare the reproductive outcomes follo...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-020-00675-z
更新日期：2020-11-20 00:00:00
abstract：:The adaptive immune system of placental mammals has evolved to tolerate the fetus. Rejection of the fetus by adaptive immune responses is therefore a rare event, with abortion being caused more frequently by inflammation in the placenta. This review will cover recent aspects of immune privilege and the innate immune s...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-1-121
更新日期：2003-12-02 00:00:00
abstract：BACKGROUND:Mammalian oocyte meiotic maturation involves a number of important processes, including spindle assembly and migration, cortical reorganization and polar body extrusion. Numerous proteins contribute to these processes, but it is unknown whether MKlp2 (mitotic kinesin-like protein 2; also called KIF20A), a mi...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-117
更新日期：2013-12-21 00:00:00
