Abstract：
:Cloning mammals by nuclear transfer is a powerful technique that is quickly advancing the development of genetically defined animal models. However, the overall efficiency of nuclear transfer is still very low and several hurdles remain before the power of this technique will be fully harnessed. Among these hurdles include an incomplete understanding of biologic processes that control epigenetic reprogramming of the donor genome following nuclear transfer. Incomplete epigenetic reprogramming is considered the major cause of the developmental failure of cloned embryos and is frequently associated with the disregulation of specific genes. At present, little is known about the developmental mechanism of reconstructed embryos. Therefore, screening strategies to design nuclear transfer protocols that will mimic the epigenetic remodeling occurring in normal embryos and identifying molecular parameters that can assess the developmental potential of pre-implantation embryos are becoming increasingly important. A crucial need at present is to understand the molecular events required for efficient reprogramming of donor genomes after nuclear transfer. This knowledge will help to identify the molecular basis of developmental defects seen in cloned embryos and provide methods for circumventing such problems associated with cloning the future application of this technology.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Li X,Li Z,Jouneau A,Zhou Q,Renard JPdoi
10.1186/1477-7827-1-84keywords:
subject
Has Abstractpub_date
2003-11-07 00:00:00pages
84issn
1477-7827pii
1477-7827-1-84journal_volume
1pub_type
杂志文章,评审abstract：BACKGROUND:The injection of estradiol valerate in female rats induces polycystic ovary syndrome, which is characterized by polycystic ovaries, anovulation, and hyperandrogenism. These characteristics have been associated with an increase in the ovarian concentration of norepinephrine, which occurs before establishing t...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0400-8
更新日期：2018-09-07 00:00:00
abstract：BACKGROUND:The G protein-coupled estrogen receptor (GPER) is thought to be involved in non-genomic estrogen responses as well as processes such as cell proliferation and migration. In this study, we analyzed GPER expression patterns from endometriosis samples and normal endometrial tissue samples and compared these exp...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-30
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journal_title：Reproductive biology and endocrinology : RB&E
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doi：10.1186/1477-7827-6-37
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abstract：BACKGROUND:Flow cytometric sorting can be used to separate sperm based on sex chromosome content. Differential fluorescence emitted by stained X- vs. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X- or Y-bearing sperm for use to influence the likelihood that the offspring will ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 临床试验,杂志文章
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更新日期：2014-11-24 00:00:00
abstract：BACKGROUND:Previous studies have demonstrated that progestin-primed ovarian stimulation (PPOS) protocol was a feasible and efficient method in in vitro fertilization (IVF) cycle. However, its application in women with advanced age has not been determined yet. The purpose of this study was to investigate its efficacy in...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-019-0518-3
更新日期：2019-11-09 00:00:00
abstract：:During rat estrous cycle, the endometrium proliferates in response to sex steroids and specific endometrial epithelial cells undergo apoptosis in absence of embryonic factors. The central executioner of apoptosis is a family of aspartic acid-specific cysteine proteases known as caspases. Smac/DIABLO is released from t...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-1-59
更新日期：2003-08-27 00:00:00
abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder associated with ovulatory dysfunction, abdominal obesity, hyperandrogenism, hypertension, and insulin resistance. METHODS:Our objectives in this study were (1) to estimate sympathetic-adrenal medullary (SAM) activity by measuring...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-3-44
更新日期：2005-09-07 00:00:00
abstract：BACKGROUND:In several species, considerably higher levels of estradiol-17 (E2) are synthesized in the CL. E2 has been suggested to participate in the regulation of luteal steroidogenesis and luteal cell morphology. In pregnant rats, several experiments have been carried out to examine the effects of inhibition of lutea...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-016-0153-1
更新日期：2016-04-12 00:00:00
abstract：BACKGROUND:Prostaglandins are important for female reproduction. Prostaglandin-E2 acts via four different receptor subtypes, EP1, EP2, EP3 and EP4 whereas prostaglandin-F2alpha acts through FP. The functions of prostaglandins depend on the expression of their receptors in different uterine cell types. Our aim was to in...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-3
更新日期：2012-01-18 00:00:00
abstract：:Although much is known about the reproductive biology of pond-breeding frogs, there is comparatively little information about terrestrial-breeding anurans, a highly successful and diverse group. This study investigates the activation and in vitro fertilization of eggs of the Puerto Rican coqui frog obtained by hormona...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-2-60
更新日期：2004-08-05 00:00:00
abstract：BACKGROUND:Brain-derived ectonucleoside triphosphate diphosphohydrolases (NTPDases) have been known as plasma membrane-incorporated enzymes with their ATP-hydrolyzing domain outside of the cell. As such, these enzymes are thought to regulate purinergic intercellular signaling by hydrolyzing ATP to ADP-AMP, thus regulat...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-7-63
更新日期：2009-06-16 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-103
更新日期：2009-09-25 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-020-00612-0
更新日期：2020-05-20 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0369-3
更新日期：2018-05-19 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-9-111
更新日期：2011-08-09 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-1-74
更新日期：2003-10-09 00:00:00
abstract：BACKGROUND:Women with Polycystic Ovary Syndrome (PCOS) present a heterogeneous reproductive and metabolic profile with an increased lifetime risk of Type 2 Diabetes (T2D). Early biomarkers of these metabolic disturbances in PCOS women have not been identified. The abundance of circulating insulin gene promotor cell-fre...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-019-0478-7
更新日期：2019-04-05 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-51
更新日期：2012-07-21 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-020-00682-0
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,多中心研究
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更新日期：2008-12-10 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-3-30
更新日期：2005-07-21 00:00:00
abstract：BACKGROUND:The aim of this study was to determine the relationship between a purported luteinizing hormone/chorionic gonadotropin (LHCGR) high function polymorphism (rs4539842/insLQ) and outcome to controlled ovarian hyperstimulation (COH). METHODS:This was a prospective study of 172 patients undergoing COH at the Fer...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-71
更新日期：2013-07-25 00:00:00
abstract：BACKGROUND:Coordinated differentiation of the endometrial compartments in the second half of the menstrual cycle is a prerequisite for the establishment of pregnancy. Endometrial stromal cells (ESC) decidualize under the influence of ovarian progesterone to accommodate implantation of the blastocyst and support establi...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-76
更新日期：2009-07-20 00:00:00
abstract：:Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced. High abortion and fetal mortality rates are commonly observed. These developmental defects have been attributed to incomple...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-1-98
更新日期：2003-11-13 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-2-81
更新日期：2004-12-10 00:00:00
abstract：BACKGROUND:hCG is a term referring to 4 independent molecules, each produced by separate cells and each having completely separate functions. These are hCG produced by villous syncytiotrophoblast cells, hyperglycosylated hCG produced by cytotrophoblast cells, free beta-subunit made by multiple primary non-trophoblastic...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
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更新日期：2010-08-24 00:00:00
abstract：BACKGROUND:The study was designed to test the hypothesis that granulosa cell (GC) gene expression response differs between recombinant FSH and human menopausal gonadotropin (hMG) stimulation regimens. METHODS:Females < 35 years-old undergoing IVF for tubal or male factor infertility were prospectively randomized to on...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/1477-7827-8-25
更新日期：2010-03-12 00:00:00
abstract：BACKGROUND:The aim of this study was to compare pregnancy rates in patients undergoing IVF/ICSI with embryo transfer after 4 and 5 days of culture in a closed incubation system with integrated time-lapse imaging. METHODS:Out of n = 2207 in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI) cycles perfo...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-017-0253-6
更新日期：2017-05-08 00:00:00
abstract：BACKGROUND:The placenta is an essential organ that provides nutrients and oxygen to the developing fetus and removes toxic waste products from the fetal circulation. Maintaining placental blood osmotic pressure and blood flow is crucial for viable offspring. The renin-angiotensin system (RAS) in the placenta is a key p...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0088-y
更新日期：2015-08-12 00:00:00
abstract：BACKGROUND:In young women with poor ovarian response, luteal-phase ovarian stimulation (LPOS) is a potential method for collecting competent oocytes. The aim of this study was to assess the efficacy of LPOS compared with follicular phase ovarian stimulation (FPOS) in young women with poor ovarian response (POR). METHO...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12958-020-00570-7
更新日期：2020-02-07 00:00:00
