Abstract：
BACKGROUND:The transformation of endometrium into decidua is essential for normal implantation of the blastocyst. However, the post-transcriptional regulation and the miRNAs involved in decidualization remain poorly understood. Here, we examined microRNA-181a (miR-181a) expression in decidualized human endometrial stromal cell (hESC). In addition, we investigated the functional effect of miR-181a on hESC decidualization in vitro. METHODS:Quantitative real-time PCR (qRT-PCR) was used to detect the profile of miR-181a in decidualized hESC. qRT-PCR, enzyme-linked fluorescent assay, and immunofluorescence assay were performed to investigate decidualization marker genes' expression after enhancing or inhibition of miR-181a expression in hESC. Luciferase reporter assay, western blotting, qRT-PCR, and immunofluorescence assay were carried out to identify the relationship between miR-181a and Krüppel-like factor 12 (KLF12). RESULTS:miR-181a expression levels increased dramatically in hESC treated with 8-Br-cAMP and MPA. Increased miR-181a expression promoted hESC decidualization-related gene expression and morphological transformation; conversely, inhibition of miR-181a expression compromised hESC decidualization in vitro. Further analysis confirmed that miR-181a interacted with the 3' untranslated region of the transcription factor KLF12 and down-regulated KLF12 at the transcriptional and translational levels. KLF12 overexpression abolished miR-181a-induced decidualization. CONCLUSIONS:Our findings suggest that miR-181a plays a functionally important role in human endometrial stromal cell decidualization in vitro by inhibiting KLF12.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Zhang Q,Zhang H,Jiang Y,Xue B,Diao Z,Ding L,Zhen X,Sun H,Yan G,Hu Ydoi
10.1186/s12958-015-0019-ysubject
Has Abstractpub_date
2015-03-26 00:00:00pages
23issn
1477-7827pii
10.1186/s12958-015-0019-yjournal_volume
13pub_type
杂志文章abstract：BACKGROUND:The overall prevalence of infertility was estimated to be 3.5-16.7% in developing countries and 6.9-9.3% in developed countries. Furthermore, according to reports from some regions of sub-Saharan Africa, the prevalence rate is 30-40%. The consequences of infertility and how it affects the lives of women in p...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-12-87
更新日期：2014-09-08 00:00:00
abstract：BACKGROUND:Pregnant women have high serum concentrations of sex steroid hormones, which are major regulators of paracrine and autocrine responses for many maternal and placental functions. The main purpose of this study was to compare patients with preeclampsia and patients with uncomplicated pregnancies in terms of se...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-020-0569-5
更新日期：2020-02-18 00:00:00
abstract：:Trophoblast cells are unique because they are one of the few mammalian cell types that do not express major histocompatibility complex (MHC) class II antigens, either constitutively or after exposure to IFN-gamma. The absence of MHC class II antigen expression on trophoblast cells has been postulated to be one of the ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-2-52
更新日期：2004-07-05 00:00:00
abstract：BACKGROUND:We found previously that the expression of SET gene was up-regulated in polycystic ovaries. Evidences suggested that SET protein was essential for regulating both the promoter activity of CYP17A1 and the biological activity of P450c17. In this study, we explored whether SET regulated androgen production in p...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-9
更新日期：2013-02-19 00:00:00
abstract：:For the extrapolation of data obtained from experimental animals to the human situation, it is important to know the similarities and differences between human and animal species. Some important characteristics of nitric oxide synthase (NOS) in myometrium and vagina from human and rat were compared. NOS-activity was m...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-1-10
更新日期：2003-02-06 00:00:00
abstract：BACKGROUND:Serum leptin levels are augmented in obese infertile men and in men with azoospermia. They also correlate inversely with sperm concentration, motility and normal forms. The mechanisms underlying the adverse effects of excess leptin on male reproductive function remain unclear. The present study aimed to eval...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0368-4
更新日期：2018-05-31 00:00:00
abstract：BACKGROUND:Age at menarche is the reflection of cumulative pre-adolescent exposure of girls to either adverse environment such as food insecurity or affluent living conditions. Food insecurity could result in inadequate nutrient intake and stress, both of which are hypothesized to have opposing effects on the timing of...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-9-125
更新日期：2011-09-13 00:00:00
abstract：BACKGROUND:Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. Th...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/1477-7827-10-72
更新日期：2012-09-06 00:00:00
abstract：BACKGROUND:Male factor infertility is quite common as 30-50% of infertility cases are due to sperm defects. The high sperm DNA fragmentation is one of the causes of male factor infertility. Many factors cause sperm DNA fragmentation and could be testicular or post-testicular. The purpose of this study was to assess rel...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0369-3
更新日期：2018-05-19 00:00:00
abstract：BACKGROUND:Pregnancy-associated plasma protein-A2 (PAPP-A2) is consistently upregulated in the placentae of pregnancies complicated by preeclampsia and fetal growth restriction. The causes and significance of this upregulation remain unknown, but it has been hypothesized that it is a compensatory response to improve pl...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0376-4
更新日期：2018-06-12 00:00:00
abstract：BACKGROUND:Puberty onset is a complex, organized biological process with multilevel regulation, and its physiopathological mechanisms are yet to be elucidated. RFRP-3, the mammalian ortholog to gonadotropin-inhibitory hormone, is implicated in inhibiting the synthesis and release of gonadotropin in mammals. However, it...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-017-0254-5
更新日期：2017-05-02 00:00:00
abstract：BACKGROUND:Antagonists of growth hormone-releasing hormone (GHRH) are being developed for the treatment of various human cancers. METHODS:MTT assay was used to test the proliferation of SKOV3 and CaOV3. The splice variant expression of GHRH receptors was examined by RT-PCR. The expression of protein in signal pathway ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-8-54
更新日期：2010-05-28 00:00:00
abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) has been recognized as a metabolic disorder, manifested by abdominal obesity, insulin resistance, dyslipidemia and hypertension. Pigment epithelium-derived factor (PEDF), a member of the serine protease inhibitor family, is a pleiotropic protein known for its antiangiogenic, ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-77
更新日期：2013-08-14 00:00:00
abstract：BACKGROUND:High levels of dopamine (DA) were described in human ovary and recently evidence for DA receptors in granulosa and luteal cells has been provided, as well. However, neither the full repertoire of ovarian receptors for DA, nor their specific role, is established. Human granulosa cells (GCs) derived from women...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-5-40
更新日期：2007-10-25 00:00:00
abstract：BACKGROUND:The aim of this study was to explore the impact of endometrial thickness change after progesterone administration on pregnancy outcome in patients transferred with single frozen-thawed blastocyst. METHODS:This observational cohort study included a total of 3091 patients undergoing their first frozen-thawed ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-019-0545-0
更新日期：2019-11-25 00:00:00
abstract：BACKGROUND:Morphometric and morphokinetic evaluation of in vitro cultured human embryos allows evaluation without time restriction and reduces intra- and inter-observer variability. Even though these technologies have been reported to improve the quality of cleavage stage embryo evaluation during fresh culture, possibl...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-017-0299-5
更新日期：2017-10-03 00:00:00
abstract：BACKGROUND:Human uterine leiomyoma (fibroids; LYO) are the most common benign neoplasms in reproductive-aged women. Dysregulated extracellular matrix and irregular LYO reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression are thought to be mediated by aberrant microRNA (miR) expression. The relat...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-016-0180-y
更新日期：2016-08-17 00:00:00
abstract：BACKGROUND:The objectives of this study were to develop an easy and rapid method for measuring gene expression in a small number of cells by real-time PCR without RNA extraction and purification, and to use this method to determine more precisely IGF-I gene expression in the cumulus cells surrounding oocytes. METHODS:...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-3-59
更新日期：2005-10-27 00:00:00
abstract：BACKGROUND:In animals, low levels of vitamin D are associated with estrus cycle disturbances, but there are virtually no human data. We examined the association of 25-hydroxyvitamin D (25(OH)D) (a biomarker for vitamin D status) with menstrual cycle characteristics. METHODS:Women aged 35-44 were randomly selected from...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0012-5
更新日期：2015-03-11 00:00:00
abstract：:The corpus luteum is an endocrine gland whose limited lifespan is hormonally programmed. This debate article summarizes findings of our research group that challenge the principle that the end of function of the corpus luteum or luteal regression, once triggered, cannot be reversed. Overturning luteal regression by ph...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-4-53
更新日期：2006-10-30 00:00:00
abstract：:Gonadotropins extracted from the urine of post-menopausal women have traditionally been used to stimulate folliculogenesis in the treatment of infertility and in assisted reproductive technology (ART). Products, such as human menopausal gonadotropin (hMG), consist not only of a mixture of the hormones, follicle-stimul...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-12-95
更新日期：2014-10-03 00:00:00
abstract：BACKGROUND:In natural cycles, women conceive when intercourse takes place during a six-day period ending on the day of ovulation. The current practice in intrauterine insemination (IUI) cycles is to perform the IUI 24-36 hours after the hCG administration, when the ovulation is already imminent. In this study hCG was a...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-8-18
更新日期：2010-02-23 00:00:00
abstract：:Establishment and maintenance of pregnancy in the pig involves intricate communication between the developing conceptuses and the maternal endometrium. This process occurs during trophoblast elongation which is spaciotemporally associated with conceptus synthesis and release of IL1B concomitant with pregnancy-specific...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-8-39
更新日期：2010-04-28 00:00:00
abstract：BACKGROUND:Relaxin levels in seminal plasma have been associated with positive effects on sperm motility and quality, and thus having potential roles in male fertility. However, the origin of seminal relaxin, within the male reproductive tract, and the moment of its release in the vicinity of spermatozoa remain unclear...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0043-y
更新日期：2015-05-20 00:00:00
abstract：BACKGROUND:During the estrous cycle, the rat uterine endometrium undergoes many changes such as cell proliferation and apoptosis. If implantation occurs, stromal cells differentiate into decidual cells and near the end of pregnancy, a second wave of apoptosis occurs. This process called decidual regression, is tightly ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-80
更新日期：2009-08-05 00:00:00
abstract：BACKGROUND:Septate uterus, one of the most common forms of congenital uterine malformations, negatively affects female reproductive health. METHODS:In a retrospective cohort study, we evaluated the reproductive outcome after hysteroscopic septoplasty in 64 women with septate uterus and primary or secondary infertility...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-8-52
更新日期：2010-05-21 00:00:00
abstract：BACKGROUND:The aim of this study was to evaluate the effects of vitrification on morpho-functional parameters (blastomere/chromatin integrity and bioenergy/oxidative potential) of mouse preimplantation embryos. METHODS:In vivo produced mouse (4/16-cell, morulae and blastocyst-stage) embryos were randomly divided into ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-27
更新日期：2013-04-03 00:00:00
abstract：:Preimplantation embryo development involves four stages: fertilization, cell cleavage, morula and blastocyst formation. During these stages, maternal and zygotic epigenetic factors play crucial roles. The gene expression profile is changed dramatically, chromatin is modified and core histone elements undergo significa...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-7-59
更新日期：2009-06-05 00:00:00
abstract：BACKGROUND:Low functional ovarian reserve (LFOR) has been associated with hypoandrogenemia and increased embryo aneuploidy, while androgen supplementation has been reported to improve aneuploidy rates. We, therefore, assessed whether in infertile women undergoing in vitro fertilization (IVF) androgen concentrations are...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0034-z
更新日期：2015-05-06 00:00:00
abstract：BACKGROUND:A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS:Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from e...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-1
更新日期：2012-01-10 00:00:00
