Abstract：
BACKGROUND:A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS:Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile women and sixteen infertile women with fallopian tubal occlusion from the Polish population. We also evaluated transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in these groups of women. RESULTS:There were significantly lower levels of HOXA11 transcripts (p = 0.003, p = 0.041) and protein (p = 0.004, p = 0.001) in women with endometriosis as compared to fertile women and infertile women with tubal occlusion. Moreover, we found significantly higher methylation levels of the CpG region in the first exon of HOXA11 in infertile women with endometriosis compared with fertile women (p < 0.001) and infertile women with tubal occlusion (p < 0.001). We also observed significantly increased levels of DNMT3A transcript in women with endometriosis than fertile women (p = 0.044) and infertile women with tubal occlusion (p = 0.047). However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women. CONCLUSIONS:We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility. Moreover, we found that DNA hypermethylation can be one of the possible molecular mechanisms causing a decrease in HOXA11 expression in the eutopic mid-secretory endometrium in infertile women with endometriosis.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Szczepańska M,Wirstlein P,Skrzypczak J,Jagodziński PPdoi
10.1186/1477-7827-10-1subject
Has Abstractpub_date
2012-01-10 00:00:00pages
1issn
1477-7827pii
1477-7827-10-1journal_volume
10pub_type
杂志文章abstract：BACKGROUND:It is not well established whether the increased number of leukocytes in the seminal fluid impairs the outcomes of assisted reproductive technology (ART). This investigation analysed the outcomes of the intracytoplasmic sperm injection (ICSI) and intracytoplasmic morphologically selected sperm injection (IMS...
journal_title：Reproductive biology and endocrinology : RB&E
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journal_title：Reproductive biology and endocrinology : RB&E
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journal_title：Reproductive biology and endocrinology : RB&E
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journal_title：Reproductive biology and endocrinology : RB&E
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journal_title：Reproductive biology and endocrinology : RB&E
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journal_title：Reproductive biology and endocrinology : RB&E
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
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更新日期：2003-12-02 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
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