Abstract:
:Gonadotropin-primed immature rats (GPIR) constitute a widely used model for the study of ovulation. Although the equivalence between the ovulatory process in immature and adult rats is generally assumed, the morphological and functional characteristics of ovulation in immature rats have been scarcely considered. We describe herein the morphological aspects of the ovulatory process in GPIR and their response to classical ovulation inhibitors, such as the inhibitor of prostaglandin (PG) synthesis indomethacin (INDO) and a progesterone (P) receptor (PR) antagonist (RU486). Immature Wistar rats were primed with equine chorionic gonadotropin (eCG) at 21, 23 or 25 days of age, injected with human chorionic gonadotropin (hCG) 48 h later, and sacrificed 16 h after hCG treatment, to assess follicle rupture and ovulation. Surprisingly, GPIR showed age-related ovulatory defects close similar to those in adult rats lacking P and PG actions. Rats primed with eCG at 21 or 23 days of age showed abnormally ruptured corpora lutea in which the cumulus-oocyte complex (COC) was trapped or had been released to the ovarian interstitum, invading the ovarian stroma and blood and lymphatic vessels. Supplementation of immature rats with exogenous P and/or PG of the E series did not significantly inhibit abnormal follicle rupture. Otherwise, ovulatory defects were practically absent in rats primed with eCG at 25 days of age. GPIR treated with INDO showed the same ovulatory alterations than vehicle-treated ones, although affecting to a higher proportion of follicles. Blocking P actions with RU486 increased the number of COC trapped inside corpora lutea and decreased ovulation. The presence of ovulatory defects in GPIR, suggests that the capacity of the immature ovary to undergo the coordinate changes leading to effective ovulation is not fully established in Wistar rats primed with eCG before 25 days of age.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Gaytan M,Bellido C,Morales C,Gonzalez-Padilla M,Sanchez-Criado JE,Gaytan Fdoi
10.1186/1477-7827-2-63keywords:
subject
Has Abstractpub_date
2004-09-03 00:00:00pages
63issn
1477-7827pii
1477-7827-2-63journal_volume
2pub_type
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-11-71
更新日期:2013-07-25 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
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更新日期:2015-07-29 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
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doi:10.1186/1477-7827-1-10
更新日期:2003-02-06 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验,收录出版
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journal_title:Reproductive biology and endocrinology : RB&E
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journal_title:Reproductive biology and endocrinology : RB&E
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更新日期:2012-12-05 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
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更新日期:2020-02-07 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
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更新日期:2009-04-19 00:00:00
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doi:10.1186/1477-7827-9-7
更新日期:2011-01-18 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
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更新日期:2005-06-08 00:00:00
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doi:10.1186/s12958-018-0368-4
更新日期:2018-05-31 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-10-20
更新日期:2012-03-21 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-018-0400-8
更新日期:2018-09-07 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
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doi:10.1186/s12958-016-0199-0
更新日期:2016-10-07 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-1-74
更新日期:2003-10-09 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
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更新日期:2005-09-07 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/1477-7827-2-31
更新日期:2004-06-16 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 临床试验,杂志文章
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更新日期:2013-06-19 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-015-0010-7
更新日期:2015-03-08 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
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更新日期:2012-09-06 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-018-0411-5
更新日期:2018-09-28 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
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更新日期:2003-03-24 00:00:00
abstract:BACKGROUND:We characterized the spectrum and etiology of hypogonadism in a cohort of Prader-Willi syndrome (PWS) adolescents and adults. METHODS:Reproductive hormonal profiles and physical examination were performed on 19 males and 16 females ages 16-34 years with PWS. Gonadotropins, sex-steroids, inhibin B (INB) and ...
journal_title:Reproductive biology and endocrinology : RB&E
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更新日期:2012-05-06 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
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更新日期:2013-03-20 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
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更新日期:2017-03-31 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
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更新日期:2004-07-05 00:00:00
abstract:BACKGROUND:Di(2-ethylhexyl) phthalate (DEHP) exposure reduces embryo implantations, increases embryonic loss, and decreases fetal body weights. However, whether it is associated with the alteration of luteal function remains unknown. Thus, our aim in this study was to explore the effect and mechanism of DEHP on luteal ...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
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更新日期:2015-03-03 00:00:00