Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1-AtMEK1 pathway by wounding.

Abstract:

:We have constructed a series of deletion mutants of Arabidopsis MAPK kinase kinase (AtMEKK1) and obtained a constitutively active mutant, AtMEKK1Delta166, which lacks in self-inhibitory sequence of N-terminal 166 amino acids but still has substrate specificity. AtMEKK1Delta166 predominantly phosphorylates AtMEK1, an Arabidopsis MAPKK, but not its double mutant (AtMEK1T218A/S224E), suggesting that Thr-218 and Ser-224 are the phosphorylation sites. In wounded seedlings, AtMEKK1 was activated and phosphorylated its downstream AtMEK1. Furthermore, analysis using anti-AtMEKK1 and anti-AtMEK1 antibodies revealed that the interaction between the two proteins was signal dependent. These results suggest the presence of AtMEKK1-AtMEK1 pathway induced by wounding.

journal_name

Planta

journal_title

Planta

authors

Hadiarto T,Nanmori T,Matsuoka D,Iwasaki T,Sato K,Fukami Y,Azuma T,Yasuda T

doi

10.1007/s00425-005-0126-7

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

708-13

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

223

pub_type

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