Involvement of a lipid-linked intermediate in the transfer of galactose from UDP [(14)C]galactose to exogenous protein in castor bean endosperm homogenates.

Abstract:

:A crude organelle preparation from germinating castor bean endosperm catalysed the incorporation of galactose from UDP[(14)C]galactose into chloroform/methanol (2:1)-soluble glactolipids. At least two galactolipids were formed. Most of the [(14)C]galactose was present in a galactolipid synthesized by the microsomal membranes, the remainder was present in a second galactolipid synthesized by other cellular membranes, possibly Golgi-derived. The addition of asialo-agalacto-fetuin reduced incorporation of [(14)C]galactose into the microsomal galactolipid with a concomitant increase in microsomal [(14)C]galactoprotein. Asialo-agalacto-fetuin did not affect galactolipid or galactoprotein synthesis by nonmicrosomal fractions. The results suggest that the endoplasmic reticulum is a major site of protein galactosylation in castor bean endosperm cells, and that galactose transfer from UDP-galactose to protein occurs via a lipid-linked intermediate.

journal_name

Planta

journal_title

Planta

authors

Mellor RB,Lord JM

doi

10.1007/BF00384596

subject

Has Abstract

pub_date

1979-10-01 00:00:00

pages

89-96

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

147

pub_type

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