Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation.


:Tea seed is believed to be recalcitrant based on its sensitivity to chilling or drying stress. Reactive oxygen species (ROS) and alterations in cytosolic redox status have been implicated in intolerance to desiccation by recalcitrant seed, but there is little information available regarding how ROS are regulated in seeds susceptible to drying stress. We investigated changes in protein expression and activity in tea embryo in response to desiccation using physiological and proteomic methods. Results showed that desiccation treatment dramatically induced the accumulation of H(2)O(2) in tea embryos, accompanied by increased activities of antioxidant enzymes like ascorbate peroxidase (APX) and superoxide dismutase (SOD). Proteomic analyses also demonstrated that 23 proteins associated with defense response, metabolism and redox status were up-regulated following desiccation. Increase in antioxidants, ascorbic acid (AsA) and catalase (CAT) (H(2)O(2) scavengers) partially assuaged desiccation damage to tea seed, resulting in improved germination rates. Higher accumulation of H(2)O(2) aggravated desiccation damage to seeds leading to lower germination activity. We propose that desiccation causes an over-accumulation of ROS that are not efficiently scavenged by increased levels of antioxidant enzymes. High levels of ROS alter the redox status and are detrimental to seed viability. Reducing ROS to appropriate concentrations is an efficient way to reduce desiccation damage and improve germination rates of recalcitrant seeds.






Chen Q,Yang L,Ahmad P,Wan X,Hu X




Has Abstract


2011-03-01 00:00:00














  • [Light dependent carotenoid synthesis : VII. Cycloheximide or anaerobic conditions as reversible blocks of the sequence of light-induced reactions].

    abstract::Anaerobic conditions completely block the synthesis of carotenoids in Fusarium aquaeduciuum or Neurospora crassa. Even after sufficient illumination of the mycelia in the presence of air, the subsequent production of carotenoids in the dark is suppressed entirely when the fungi are transferred to an oxygen-free atmosp...


    pub_type: 杂志文章


    authors: Rau W

    更新日期:1971-09-01 00:00:00

  • Aspects of phytochrome decay in etiolated seedlings under continuous Illumination.

    abstract::The rate of total phytochrome decay in the dicotyledons Amaranthus caudatus, Mirabilis jalapa and Pisum sativum under continuous illumination with red, incandescent, and blue light depends on the PFR/Ptotal maintained by each source. Amaranthus is an exception to this in that there is a deviation from firstorder decay...


    pub_type: 杂志文章


    authors: Kendrick RE

    更新日期:1972-12-01 00:00:00

  • [The diffusion resistance of stomata: a comparison between the CO2-exchange of normal and stripped leaves].

    abstract::The lower epidermis from leaves of Primula palinuri can be stripped off. Light-saturation curves of the CO2-exchange were measured at 20°C and 300 ppm CO2. Whereas the normal leaf reaches light-saturation at 0.3 cal cm(-2) min(-1), even 0.6 cal cm(-2) min(-1) is not sufficient to saturate the stripped leaf. Transpirat...


    pub_type: 杂志文章


    authors: Bertsch A

    更新日期:1969-03-01 00:00:00

  • Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line.

    abstract::A heat-tolerant maize (Zea mays L.) line, ZPBL 1304, synthesizes a unique set of five heat-shock polypeptides of 45 kDa. Previous studies suggested that these polypeptides might play a role in the development of thermotolerance in maize (Ristic et al., 1996, J. Plant Physiol. 149:424-432; Ristic et al., 1998, J. Plant...


    pub_type: 杂志文章


    authors: Bhadula SK,Elthon TE,Habben JE,Helentjaris TG,Jiao S,Ristic Z

    更新日期:2001-02-01 00:00:00

  • Identification and characterization of phospholipase D and its association with drought susceptibilities in peanut (Arachis hypogaea).

    abstract::Preharvest aflatoxin contamination has been identified by the peanut industry as a serious issue in food safety and human health because of the carcinogenic toxicity. Drought stress is the most important environmental factor exacerbating Aspergillus infection and aflatoxin contamination in peanut. The development of d...


    pub_type: 杂志文章


    authors: Guo BZ,Xu G,Cao YG,Holbrook CC,Lynch RE

    更新日期:2006-02-01 00:00:00

  • Fluxes and compartmentation of potassium and chloride in the green alga Mougeotia.

    abstract::Some ionic relations of the filamentous green alga Mougeotia sp. have been analyzed under different light conditions. Data from influx and efflux measurements using (86)Rb(+) and (36)Cl(-) fit the model of three cellular compartments (cell wall, cytoplasm, vacuole) in series. This result is remarkable, since in a Moug...


    pub_type: 杂志文章


    authors: Wagner G

    更新日期:1974-06-01 00:00:00

  • The localization of enzymes in the cotyledons of Pisum arvense L. during germination.

    abstract::Enzyme activity is not uniformly distributed through the cotyledon of Pisum arvense. Initially the peripheral region, certain scattered cells of the storage tissue and the procambium show a high level of activity of succinic dehydrogenase, cytochrome oxidase, acid phosphatase and esterase. Activity of acid phosphatase...


    pub_type: 杂志文章


    authors: Flinn AM,Smith DL

    更新日期:1967-03-01 00:00:00

  • The evolutionary landscape of PRC1 core components in green lineage.

    abstract:MAIN CONCLUSION:The origin and evolution of plant PRC1 core components. Polycomb repressive complex1 (PRC1) plays critical roles in epigenetic silencing of homeotic genes and determination of cell fate. Animal PRC1 has been well investigated for a long time, whereas plant PRC1 was just confirmed in recent years. It is ...


    pub_type: 杂志文章,评审


    authors: Chen DH,Huang Y,Ruan Y,Shen WH

    更新日期:2016-04-01 00:00:00

  • Effects of seasonal temperature changes on DkMyb4 expression involved in proanthocyanidin regulation in two genotypes of persimmon (Diospyros kaki Thunb.) fruit.

    abstract::Persimmon fruits accumulate a large amount of proanthocyanidin (PA). Fruits of the mutant non-astringent (NA) type lose their ability to accumulate PA at an early stage of fruit development, whereas fruits of the normal astringent (A) type sustain PA accumulation until ripening. This allelotype is determined by the ge...


    pub_type: 杂志文章


    authors: Akagi T,Tsujimoto T,Ikegami A,Yonemori K

    更新日期:2011-05-01 00:00:00

  • Photosensitized inhibitor formation in isolated, aging chloroplasts.

    abstract::The possible mechanism which leads to inactivation phenomena at high intensities of blue light during the color-sensitive phase in the time course of the Hill reaction was investigated in more detail. The following results were obtained: a) The inhibition of the Hill reaction is also observed during but not prior to t...


    pub_type: 杂志文章


    authors: Harnischfeger G

    更新日期:1972-12-01 00:00:00

  • Carotenoid gene expression explains the difference of carotenoid accumulation in carrot root tissues.

    abstract::Main conclusion Variations in gene expression can partially explain the difference of carotenoid accumulation in secondary phloem and xylem of fleshy carrot roots. The carrot root is well divided into two different tissues separated by vascular cambium: the secondary phloem and xylem. The equilibrium between these two...


    pub_type: 杂志文章


    authors: Perrin F,Hartmann L,Dubois-Laurent C,Welsch R,Huet S,Hamama L,Briard M,Peltier D,Gagné S,Geoffriau E

    更新日期:2017-04-01 00:00:00

  • Application of 1H-NMR combined with qRT-PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib.

    abstract:MAIN CONCLUSION:Methyl jasmonate promotes the synthesis of rosmarinic acid in Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib, and it promotes the latter more strongly. Salvia miltiorrhiza Bunge (SMB) is a traditional Chinese medicinal material, its water-soluble phenolic acid component rosmarinic acid ...


    pub_type: 杂志文章


    authors: Hou Z,Li Y,Su F,Chen J,Zhang X,Xu L,Yang D,Liang Z

    更新日期:2020-11-27 00:00:00

  • Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae).

    abstract:MAIN CONCLUSION:Our study demonstrated that symplasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of cell d...


    pub_type: 杂志文章


    authors: Wróbel-Marek J,Kurczyńska E,Płachno BJ,Kozieradzka-Kiszkurno M

    更新日期:2017-03-01 00:00:00

  • NAC103, a NAC family transcription factor, regulates ABA response during seed germination and seedling growth in Arabidopsis.

    abstract:MAIN CONCLUSION:The Arabidopsis transcription factor NAC103 is up-regulated and its encoding protein is stabilized by ABA treatment, which positively regulates several ABA-responsive downstream genes during seed germination and seedlings growth. The Arabidopsis transcription factor NAC103 was previously found to be inv...


    pub_type: 杂志文章


    authors: Sun L,Liu LP,Wang YZ,Yang L,Wang MJ,Liu JX

    更新日期:2020-10-31 00:00:00

  • Assessment of nutrient remobilization through structural changes of palisade and spongy parenchyma in oilseed rape leaves during senescence.

    abstract:MAIN CONCLUSION:Differential palisade and spongy parenchyma structural changes in oilseed rape leaf were demonstrated. These dismantling processes were linked to early senescence events and associated to remobilization processes. During leaf senescence, an ordered cell dismantling process allows efficient nutrient remo...


    pub_type: 杂志文章


    authors: Sorin C,Musse M,Mariette F,Bouchereau A,Leport L

    更新日期:2015-02-01 00:00:00

  • The intron of the Arabidopsis thaliana COX5c gene is able to improve the drought tolerance conferred by the sunflower Hahb-4 transcription factor.

    abstract::Hahb-4 is a member of Helianthus annuus (sunflower) subfamily I of HD-Zip proteins. Transgenic Arabidopsis thaliana plants constitutively expressing this gene exhibit a strong tolerance of water stress in concert with morphological defects and a delay in development. In order to obtain a drought-tolerant phenotype wit...


    pub_type: 杂志文章


    authors: Cabello JV,Dezar CA,Manavella PA,Chan RL

    更新日期:2007-10-01 00:00:00

  • The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta.

    abstract::In the genus Petunia, distinct pollination syndromes may have evolved in association with bee-visitation (P. integrifolia spp.) or hawk moth-visitation (P. axillaris spp). We investigated the extent of congruence between floral fragrance and olfactory perception of the hawk moth Manduca sexta. Hawk moth pollinated P. ...


    pub_type: 杂志文章


    authors: Hoballah ME,Stuurman J,Turlings TC,Guerin PM,Connétable S,Kuhlemeier C

    更新日期:2005-09-01 00:00:00

  • Conyza species: distribution and evolution of multiple target-site herbicide resistances.

    abstract:MAIN CONCLUSION:Distribution of Conyza species is well correlated with human interference. Multiple herbicide resistance is caused by the attempt to overcome resistance to one mode of action by overuse of another. Conyza canadensis (CC) and Conyza bonariensis (CB) are troublesome weeds around the world. Extensive use o...


    pub_type: 杂志文章


    authors: Matzrafi M,Lazar TW,Sibony M,Rubin B

    更新日期:2015-07-01 00:00:00

  • Evaluation of the Escherichia coli threonine deaminase gene as a selectable marker for plant transformation.

    abstract::The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to alpha-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile. Mutations in TD that manifest insensitivity to Ile feedback inhibition result in intracellular accumulation of Ile. P...


    pub_type: 杂志文章


    authors: Ebmeier A,Allison L,Cerutti H,Clemente T

    更新日期:2004-03-01 00:00:00

  • Regulation and activation of phytoene synthase, a key enzyme in carotenoid biosynthesis, during photomorphogenesis.

    abstract::During photomorphogenesis in higher plants, a coordinated increase occurs in the chlorophyll and carotenoid contents. The carotenoid level is under phytochrome control, as reflected by the light regulation of the mRNA level of phytoene synthase (PSY), the first enzyme in the carotenoid biosynthetic pathway. We investi...


    pub_type: 杂志文章


    authors: Welsch R,Beyer P,Hugueney P,Kleinig H,von Lintig J

    更新日期:2000-11-01 00:00:00

  • [An attempt at a causal analysis of the geotropical reaction chain in the Chara rhizoid].

    abstract::In the initial phase of the geotropical reaction of the Chara rhizoid the growth difference postulated by Sievers (1967c) between the physically upper, slightly subapical flank and the lower one is demonstrated. In horizontal exposure the growth of the extreme cell apex is continued, while the growth of the lower flan...


    pub_type: 杂志文章


    authors: Sievers A,Schröter K

    更新日期:1971-12-01 00:00:00

  • Rates of synthesis and source of glycolate in intact chloroplasts.

    abstract::Intact chloroplasts capable of high rates of CO2 assimilation completely oxidized 3-phosphoglycerate and dihydroxyacetone phosphate to glycolate when CO2 concentrations were low. Bicarbonate was converted first into products of the Calvin cycle and then into glycolate. Under high oxygen and at high pH values CO2 fixat...


    pub_type: 杂志文章


    authors: Kirk MR,Heber U

    更新日期:1976-01-01 00:00:00

  • Chloroplast genome assembly of Handroanthus impetiginosus: comparative analysis and molecular evolution in Bignoniaceae.

    abstract:MAIN CONCLUSION:Bignoniaceae species have conserved chloroplast structure, with hotspots of nucleotide diversity. Several genes are under positive selection, and can be targets for evolutionary studies. Bignoniaceae is one of the most species-rich family of woody plants in Neotropical seasonally dry forests. Here we re...


    pub_type: 杂志文章


    authors: Sobreiro MB,Vieira LD,Nunes R,Novaes E,Coissac E,Silva-Junior OB,Grattapaglia D,Collevatti RG

    更新日期:2020-10-24 00:00:00

  • The role of carbon dioxide and oxygen in determining chlorophyll fluorescence quenching during leaf development.

    abstract::Chlorophyll fluorescence emission at 680 nm (F680) and the rate of CO2 fixation were measured simultaneously in sections along the length of wheat and maize leaves. These leaves possess a basal meristem and show a gradation in development towards the leaf tip. The redox state of the primary electron acceptor, Q, of ph...


    pub_type: 杂志文章


    authors: Ireland CR,Baker NR,Long SP

    更新日期:1985-09-01 00:00:00

  • The immuno-histochemical localization of lectin in pea seeds (Pisum sativum L.).

    abstract::The lectin from the garden pea (Pisum sativum L.) has been localized at the ultrastructural level by the unlabeled peroxidase-antiperoxidase procedure of L.A. Sternberger et al. (1970, J. Histochem. Cytochem 18, 315-333) in 24 h imbibed seeds. Upon examination by light microscopy and transmission electron microscopy, ...


    pub_type: 杂志文章


    authors: Van Driessche E,Smets G,Dejaegere R,Kanarek L

    更新日期:1981-12-01 00:00:00

  • Light-dependent hydrogen evolution by Scenedesmus.

    abstract::The effect of glucose and the uncoupler Cl-CCP upon hydrogen production was studied in adapted cells of Scenedesmus obliquus D3. Cl-CCP at 10(-5)M concentration completely inhibited the evolution of H2 in the dark and increased the apparent rate of H2 evolution in the light. At 10(-5)M Cl-CCP, photosynthesis and photo...


    pub_type: 杂志文章


    authors: Kaltwasser H,Stuart TS,Gaffron H

    更新日期:1969-12-01 00:00:00

  • Photosynthetic parameters of sexually different parts of Porphyra katadai var. hemiphylla (Bangiales, Rhodophyta) during dehydration and re-hydration.

    abstract::Physiological data from extreme habitat organisms during stresses are vital information for comprehending their survival. The intertidal seaweeds are exposed to a combination of environmental stresses, the most influential one being regular dehydration and re-hydration. Porphyra katadai var. hemiphylla is a unique int...


    pub_type: 杂志文章


    authors: Lin AP,Wang GC,Yang F,Pan GH

    更新日期:2009-03-01 00:00:00

  • Inhibition of photosynthesis of sunflower leaves by an endogenous solute and interdependence of different photosynthetic reactions.

    abstract::Photosynthesis of Helianthus annuus L. leaves was transiently inhibited and respiration was stimulated when a leaf was detached from the plant by cutting the petiole under water. These effects were caused by a solute which was released by cutting and was transported by the transpiration stream to the leaf blade. This ...


    pub_type: 杂志文章


    authors: Gsell W,Kiirats O,Hartung W,Heber U

    更新日期:1989-03-01 00:00:00

  • Phytochrome-mediated regulation of β-amylase mRNA level in mustard (Sinapis alba L.) cotyledons.

    abstract::Phytochrome, activated by continuous red light, increases the amount of total polyadenylated RNA during photomorphogenesis of mustard (Sinapis alba L.) cotyledons. In-vitro translation of total polyadenylated RNA in a reticulocyte translation system has shown that the activity of translatable β-amylase mRNA is increas...


    pub_type: 杂志文章


    authors: Sharma R,Schopfer P

    更新日期:1987-07-01 00:00:00

  • Temperature dependence of intracellular pH in higher plant cells : A (31)P nuclear magnetic resonance study on maize root tips.

    abstract::The recent introduction of (31)P nuclear magnetic resonance spectroscopy offers a new approach to the problem of obtaining a simultaneous and direct evaluation of both the cytoplasmic and vacuolar pH in higher plant cells (J. K. M. Roberts, P.M. Ray, N. Waderlardetzky and O. Sardetzky, 1980, Nature 283, 870-872; 1981,...


    pub_type: 杂志文章


    authors: Aducci P,Federico R,Carpinelli G,Podo F

    更新日期:1982-01-01 00:00:00