Diversity in subcellular targeting of the PP2A B'eta subfamily members.

Abstract:

:Protein phosphatase 2A (PP2A) is a serine/threonine-specific phosphatase comprising a catalytic subunit (C), a scaffolding subunit (A), and a regulatory subunit (B). The B subunits are believed to be responsible for substrate specificity and localization of the PP2A complex. In plants, three families of B subunits exist, i.e. B (B55), B', and B''. Here, we report differential subcellular targeting within the Arabidopsis B'eta subfamily, which consists of the close homologs B'eta, B'theta, B'gamma and B'zeta. Phenotypes of corresponding knockouts were observed, and particularly revealed delayed flowering for the B'eta knockout. The B' subunits were linked to fluorescent tags and transiently expressed in various tissues of onion, tobacco and Arabidopsis. B'eta and B'gamma targeted the cytosol and nucleus. B'zeta localized to the cytoplasm and partly co-localized with mitochondrial markers when the N-terminus was free. Provided its C-terminus was free, the B'theta subunit targeted peroxisomes. The importance of the C-terminal end for peroxisomal targeting was further confirmed by truncation of the C-terminus. The results revealed that the closely related B' subunits are targeting different organelles in plants, and exemplify the usage of the peptide serine-serine-leucine as a PTS1 peroxisomal signaling peptide.

journal_name

Planta

journal_title

Planta

authors

Matre P,Meyer C,Lillo C

doi

10.1007/s00425-009-0998-z

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

935-45

issue

5

eissn

0032-0935

issn

1432-2048

journal_volume

230

pub_type

杂志文章

相关文献

PLANTA文献大全
  • In vivo photoinactivation of ribulose bisphosphate carboxylase in the gas-vacuolate cyanobacteriumMicrocystis aeruginosa.

    abstract::Irradiation of buoyant, gas-vacuolate cells of the cyanobacteriumMicrocystis aeruginosa by 5·10(4) Wm(-2) of blue light for 1 h caused a 5% loss of extractable ribulose bisphosphate carboxylase activity compared to dark and red-light controls. Ribulose bisphosphate carboxylase activity was unaffected by blue light in ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00571178

    authors: Stewart R,Codd GA

    更新日期:1980-01-01 00:00:00

  • Circular RNA architecture and differentiation during leaf bud to young leaf development in tea (Camellia sinensis).

    abstract:MAIN CONCLUSION:Circular RNA (circRNA) discovery, expression patterns and experimental validation in developing tea leaves indicates its correlation with circRNA-parental genes and potential roles in ceRNA interaction network. Circular RNAs (circRNAs) have recently emerged as a novel class of abundant endogenous stable...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2983-x

    authors: Tong W,Yu J,Hou Y,Li F,Zhou Q,Wei C,Bennetzen JL

    更新日期:2018-12-01 00:00:00

  • Cell-type-specific expression of a wheat-germ agglutinin gene in embryos and young seedlings of Triticum aestivum.

    abstract::We have investigated the levels of cell-specific expression of wheat-germ agglutinin (WGA) during the development of embryos and in 3-d-old seedlings. Southern blot analysis of genomic DNA derived from hexaploid and diploid wheat (Triticum) species indicates that the isolated cDNA (complementary DNA) clone is specific...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00395422

    authors: Raikhel NV,Bednarek SY,Wilkins TA

    更新日期:1988-12-01 00:00:00

  • Isolation and functional characterization of a floral repressor, BcFLC2, from Pak-choi (Brassica rapa ssp. chinensis).

    abstract:MAIN CONCLUSION:BcFLC2 functioned as a repressor of flowering by directly regulating BcTEM1, BcMAF2, BcSOC1 and BcSPL15 in Pak-choi. FLOWERING LOCUS C (FLC) plays an important role in regulating flowering time. Here, we functionally described an FLC homologous gene, BcFLC2, that negatively regulated flowering in Pak-ch...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2891-0

    authors: Huang F,Liu T,Wang J,Hou X

    更新日期:2018-08-01 00:00:00

  • Rapid induction of ethylene biosynthesis in cultured parsley cells by fungal elicitor and its relationship to the induction of phenylalanine ammonia-lyase.

    abstract::The biosynthesis of ethylene was examined in suspension-cultured cells of parsley (Petroselinum hortense) treated with an elicitor from cell walls of Phytophthora megasperma. Untreated cells contained 50 nmol g(-1) of the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), and produced ethylene at a rate ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394581

    authors: Chappell J,Hahlbrock K,Boller T

    更新日期:1984-07-01 00:00:00

  • Localization and uptake of 14C-IAA in relation to xylem regeneration in Coleus internodes.

    abstract::14C-IAA was applied to decapitated Coleus 5th internodes. Sections were radioautographed at various intervals after the application. The location of the label within cells and tissues is discussed. No accumulation of label occurred in the region where xylem cells regenerated. Label was particularly associated with xyl...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386501

    authors: Gee H

    更新日期:1972-03-01 00:00:00

  • Molecular cloning and characterization of CrNCED1, a gene encoding 9-cis-epoxycarotenoid dioxygenase in Citrus reshni, with functions in tolerance to multiple abiotic stresses.

    abstract::Abscisic acid (ABA) is an important stress phytohormone that plays an essential role in mediating the signaling networks associated with plant responses to various abiotic stresses. In the present study, we isolated a gene CrNCED1 encoding the rate-limiting enzyme of ABA synthesis, 9-cis-epoxycarotenoid dioxygenase (N...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1963-4

    authors: Xian L,Sun P,Hu S,Wu J,Liu JH

    更新日期:2014-01-01 00:00:00

  • Mass isolation of plant chromosomes and nuclei.

    abstract::A method is presented for mass isolation of metaphase chromosomes and nuclei from plant protoplasts. The isolation procedure was developed for both monocotyledonous and dicotyledonous species using wheat (Triticum monococcum) and poppy (Papaver somniferum) cell cultures. Metaphase chromosomes were isolated from partia...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00405195

    authors: Hadlaczky G,Bisztray G,Praznovszky T,Dudits D

    更新日期:1983-04-01 00:00:00

  • Distribution and accumulation of ultraviolet-radiation-absorbing compounds in leaves of tropical mangroves.

    abstract::Ultraviolet (UV)-absorbing phenolic compounds that have been shown to be protective against the damaging: effects of UV-B radiation (Tevini et al., 1991, Photochem. Photobiol. 53, 329-333) were found in the leaf epidermis of tropical mangrove tree species. These UV-absorbing phenolic compounds and leaf succulence func...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00216808

    authors: Lovelock CE,Clough BF,Woodrow IE

    更新日期:1992-09-01 00:00:00

  • The influence of inorganic nitrogen supply on carbohydrate and related metabolism in the blue-green alga, Anabaena cylindrica Lemm.

    abstract::Enzymes representative of, and related to, the pentose phosphate pathway, glycolysis, and the tricarboxylic acid cycle have been demonstrated in supernatant and lamellar fractions of Anabaena cylindrica cultured in the presence of atmospheric nitrogen, ammonia, nitrite, and nitrate. Nitrogen-fixing and ammonia-assimil...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390227

    authors: Batt T,Brown DH

    更新日期:1974-09-01 00:00:00

  • Expression of the ethylene response factor gene TSRF1 enhances abscisic acid responses during seedling development in tobacco.

    abstract::Ethylene response factor (ERF) proteins function as multiple regulators in the interaction of different stress-responsive pathways. During investigating the interaction of ethylene and abscisic acid (ABA) pathways, several GCC-box-binding repressors of ERF proteins have been reported to repress both ethylene- and ABA-...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-008-0779-0

    authors: Zhang H,Yang Y,Zhang Z,Chen J,Wang XC,Huang R

    更新日期:2008-10-01 00:00:00

  • A method for high-frequency intergeneric fusion of plant protoplasts.

    abstract::Protoplasts of Vicia hajastana Grossh. obtained from suspension-culture cells and Pisum sativum L. obtained from leaves adhered tightly to each other in concentrated solutions of high-molecular-weight polyethylene glycol (PEG). The adhesion occurred non-specifically between the free protoplasts from the same species a...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388618

    authors: Kao KN,Michayluk MR

    更新日期:1974-12-01 00:00:00

  • Role of hormones in controlling vascular differentiation and the mechanism of lateral root initiation.

    abstract::The vascular system in plants is induced and controlled by streams of inductive hormonal signals. Auxin produced in young leaves is the primary controlling signal in vascular differentiation. Its polar and non-polar transport pathways and major controlling mechanisms are clarified. Ethylene produced in differentiating...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-013-1927-8

    authors: Aloni R

    更新日期:2013-11-01 00:00:00

  • Purification and immunolocalization of an annexin-like protein in pea seedlings.

    abstract::As part of a study to identify potential targets of calcium action in plant cells, a 35-kDa, annexin-like protein was purified from pea (Pisum sativum L.) plumules by a method used to purify animal annexins. This protein, called p35, binds to a phosphatidylserine affinity column in a calcium-dependent manner and binds...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00201617

    authors: Clark GB,Dauwalder M,Roux SJ

    更新日期:1992-04-01 00:00:00

  • Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus : II. Auxotrophic and temperature-sensitive clones.

    abstract::The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated after screening a total of 29,000 clones. Two are auxotrophic fo...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385321

    authors: Gebhardt C,Schnebli V,King PJ

    更新日期:1981-10-01 00:00:00

  • Cristal mutations in Arabidopsis confer a genetically heritable, recessive, hyperhydric phenotype.

    abstract::A new class of recessive Arabidopsis mutants, designated cristal (cri) has been isolated which display several abnormalities reminiscent of hyperhydric symptoms. These characteristics include translucent and wrinkled cotyledons and leaves, abnormal chloroplast organization, a reduced amount of chlorophyll, a reduced d...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050102

    authors: Delarue M,Santoni V,Caboche M,Bellini C

    更新日期:1997-01-01 00:00:00

  • In vitro synthesis of the cyanelle proteins of Cyanophora paradoxa by isolated cyanelles and cyanelle RNA.

    abstract::Cyanelles isolated from the alga Cyanophora paradoxa Korschikoff synthesized cyanelle proteins in vitro. This synthesis was stimulated by light and totally inhibited by chloramphenicol. Cycloheximide had only a small inhibitory effect. Electrophoretic separation of the labelled soluble cyanelle proteins yielded at lea...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394488

    authors: Jehn U,Zetsche K

    更新日期:1988-01-01 00:00:00

  • Lipid metabolism in green leaves of developing monocotyledons.

    abstract::Lipid synthesis was studied in successive leaf sections from the base to the tip of developing barley (Hordeum vulgare L.), maize (Zea mays L.), rye grass (Lolium perenne L.) and wheat (Triticum aestivum L.) leaves. The endogenous levels of acyl lipids and their constituent fatty acids from the same leaf sections were...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388640

    authors: Bolton P,Harwood JL

    更新日期:1978-01-01 00:00:00

  • Scanning electron-microscopy of abscission-zone surfaces of Valencia-orange fruit.

    abstract::The scanning electron-microscope was used to obtain a more adequate view of the separation surfaces exposed when the stem was pulled from the fruit of Valencia orange. Separations were made at 0, 24 and 48 h after ethylene treatment of the fruit. The most noticeable differences are the lessened amount of tearing in pr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390215

    authors: Rogers BJ

    更新日期:1971-12-01 00:00:00

  • Ultrastructure of fusion products from soybean cell culture and sweet clover leaf protoplasts.

    abstract::Protoplasts from cultured cells of soybean (Glycine max L.) and from sweet clover (Melilotus officinalis L.) mesophyll cells were fused with polyethylene glycol and subsequently cultured for six days. The resulting fusion products as well as unfused protoplasts of each parental species regenerated cell walls and divid...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390842

    authors: Fowke LC,Rennie PJ,Kirkpatrick JW,Constabel F

    更新日期:1976-01-01 00:00:00

  • Sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of Vicia narbonensis L.

    abstract::In order to change the sugar status during seed development a yeast-derived invertase gene was expressed in cotyledons of Vicia narbonensis. As a result, sucrose decreased whereas hexoses accumulated. We analysed cell structure and cellular differentiation in cotyledons expressing the yeast-invertase. Transgenic cells...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000305

    authors: Neubohn B,Gubatz S,Wobus U,Weber H

    更新日期:2000-08-01 00:00:00

  • Can cell walls bending round xylem vessels control water flow?

    abstract::Vascular bundles of petioles below wilted leaves of Nymphoides peltata (S.G. Gmel. O. Kuntze) were frozen intact and freeze-fractured for electron microscopy. Cell walls in them appeared drawn in against the helical thickenings of xylem vessels. By contrast, walls round vessels which had been frozen in vascular bundle...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385984

    authors: Johnson RP

    更新日期:1977-01-01 00:00:00

  • Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana.

    abstract::In plants, chlorophyll is actively synthesized from glutamate in the developmental phase and is degraded into non-fluorescent chlorophyll catabolites during senescence. The chlorophyll metabolism must be strictly regulated because chlorophylls and their intermediate molecules generate reactive oxygen species. Many mec...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-010-1099-8

    authors: Nagane T,Tanaka A,Tanaka R

    更新日期:2010-03-01 00:00:00

  • Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and desiccation environments.

    abstract::The effects of O2, growth-regulators and desiccation on callus growth and somatic embryo (embryoid) development were investigated in cultures of immature embryos of two lines of Triticum aestivum L. Callus and embryoid formation were induced on media that contained N(6)-furfurylamin-opurine (kinetin) and either 2,4-di...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00396349

    authors: Carman JG

    更新日期:1988-09-01 00:00:00

  • Transcriptomic changes in wind-exposed poplar leaves are dependent on developmental stage.

    abstract::Responses of plant tissue to environmental challenges can vary among different plant parts and among plants of different ages. Investment into defense has been proposed to be influenced by fitness value and/or allocation of available resources. Here we show at first time at transcriptome level that plant defense is no...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-008-0777-2

    authors: Fluch S,Olmo CC,Tauber S,Stierschneider M,Kopecky D,Reichenauer TG,Matusíková I

    更新日期:2008-10-01 00:00:00

  • Calcium influx at the plasmalemma of Chara corallina.

    abstract::Influx of (45)Ca into internodal cells of Chara corallina has been measured, using short uptake times, and a wash in ice-cold La(3+)-containing pondwater after the labelling period to overcome the difficulty of distinguishing extracellular tracer from that in the cell. Over 5-15 min the uptake was linear with time, th...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392485

    authors: Macrobbie EA,Banfield J

    更新日期:1988-11-01 00:00:00

  • Atomic force microscopy and laser confocal scanning microscopy analysis of callose fibers developed from protoplasts of embryogenic cells of a conifer.

    abstract::Efficiency of novel fiber formation was much improved in protoplast culture of embryogenic cells (ECs) of a conifer, Larix leptolepis (Sieb. et Zucc.) Gord., by pre-culturing ECs in a medium containing a high concentration of glutamine (13.7 mM). The fibrillar substructures of large and elongated fibers of protoplasts...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-0065-3

    authors: Fukumoto T,Hayashi N,Sasamoto H

    更新日期:2005-12-01 00:00:00

  • Stimulation of precursor rRNA synthesis in the cotyledons of mustard seedlings by phytochrome.

    abstract::Cotyledons of mustard (Sinapis alba L.) seedlings irradiated with far-red light were shown to synthesize high molecular weight precursor rRNA (2.4×10(6)-2.9×10(6) dalton) at a considerably enhanced rate compared to dark-grown cotyledons. This result was obtained by pulse-labelling cotyledons isolated from 48 h old see...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384765

    authors: Thien W,Schopfer P

    更新日期:1975-01-01 00:00:00

  • Gas chromatographic-mass spectroscopic identification and quantification of arachidonic acid in wheat-germ oil.

    abstract::Gas chromatographic-mass spectroscopic evidence is presented for the presence of arachidonic acid ((all-Z)-5,8,11,14-eicosatetraenoic acid) in virgin wheat-germ oil. The amount of arachidonic acid in wheat-germ oil, determined as methyl ester using gas chromatographic detection, was quantified with reference to a stan...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00429462

    authors: Janistyn B

    更新日期:1982-08-01 00:00:00

  • Lipoxygenase-generated hydroperoxides account for the nonphysiological features of ethylene formation from 1-aminocyclopropane-1-carboxylic acid by microsomal membranes of carnations.

    abstract::Several lines of evidence indicate that the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by microsomal membranes from carnation flowers is attributable to hydroperoxides generated by membrane-associated lipoxygenase (EC 1.13.11.12). As the flowers senesce, the capability of isolated microsomal...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391036

    authors: Lynch DV,Sridhara S,Thompson JE

    更新日期:1985-05-01 00:00:00