Abstract:
:The presence of selectable marker genes and vector backbone sequences has affected the safe assessment of transgenic plants. In this study, the ovary-drip method for directly generating vector- and selectable marker-free transgenic plants was described, by which maize was transformed with a linear GFP cassette (Ubi-GFP-nos). The key features of this method center on the complete removal of the styles and the subsequent application of a DNA solution directly to the ovaries. The movement of the exogenous DNA was monitored using fluorescein isothiocyanate-labeled DNA, which showed that the time taken by the exogenous DNA to enter the ovaries was shortened compared to that of the pollen-tube pathway. This led to an improved transformation frequency of 3.38% compared to 0.86% for the pollen-tube pathway as determined by PCR analysis. The use of 0.05% surfactant Silwet L-77 + 5% sucrose as a transformation solution further increased the transformation frequency to 6.47%. Southern blot analysis showed that the transgenic plants had low transgene copy number and simple integration pattern. Green fluorescence was observed in roots and immature embryos of transgenic plants by fluorescence microscopy. Progeny analysis showed that GFP insertions were inherited in T(1) generation. The ovary-drip method would become a favorable choice for directly generating vector- and marker-free transgenic maize expressing functional genes of agronomic interest.
journal_name
Plantajournal_title
Plantaauthors
Yang A,Su Q,An Ldoi
10.1007/s00425-008-0871-5subject
Has Abstractpub_date
2009-03-01 00:00:00pages
793-801issue
4eissn
0032-0935issn
1432-2048journal_volume
229pub_type
杂志文章相关文献
PLANTA文献大全abstract::At high potassium concentrations it is possible to isolate largely intact polysomes from fertilized and unfertilized Fucus vesiculosus eggs, and to study the distribution pattern of ribosomal particles in a sucrose gradient. The changes in the ribosomal pattern before and after fertilization reflect the time course of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388548
更新日期:1969-06-01 00:00:00
abstract::The diploid woodland strawberry, Fragaria vesca, is being recognized as a model for the more complex octoploid commercial strawberry, Fragaria × ananassa. F. vesca exhibits a short seed to seed cycle, can be easily transformed by Agrobacteria, and a draft genome sequence has been published. These features, together wi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1562-1
更新日期:2012-06-01 00:00:00
abstract::The circadian movement of the lamina of primary leaves of Phaseolus coccineus L. is mediated by antagonistic changes in the length of the extensor and flexor cells of the laminar pulvinus. The cortex of the pulvinus is a concentric structure composed of hexagonal disc-like cells, arranged in longitudinal rows around t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395147
更新日期:1985-03-01 00:00:00
abstract::Homeotic changes played a considerable role during the evolution of flowers, but how floral homeotic mutants initially survive in nature has remained enigmatic. To better understand the evolutionary potential of floral homeotic mutants, we established as a model system Stamenoid petals (Spe), a natural variant of Caps...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-1018-z
更新日期:2009-11-01 00:00:00
abstract::Two actin genes, actA from the hemibiotrophic anthracnose fungus, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. f. sp. malvae, and act1 from its host, Malva pusilla (Sm.) were cloned from a cDNA library developed from infected host tissue. The actin gene, actA, of C. gloeosporioides f. sp. malvae, which is simi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050752
更新日期:1999-10-01 00:00:00
abstract:MAIN CONCLUSION:4-coumarate-CoA ligase (VIT_02s0109g00250) and copper amine oxidase (VIT_17s0000g09100) played essential roles in contributing to the total soluble solid and total anthocyanin variations induced by bud sport in grape berries. Taste and color, which are important organoleptic qualities of grape berry, un...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03543-7
更新日期:2021-01-05 00:00:00
abstract::Cadmium contamination is a serious environmental problem for modern agriculture and human health. Salinity affects plant growth and development, and interactions between salt and cadmium have been reported. However, the molecular mechanisms of salinity-cadmium interactions are not fully understood. Here, we show that ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-1070-8
更新日期:2010-01-01 00:00:00
abstract::A 21-kDa calmodulin (CaM)-binding protein and a 19-kDa calmodulin-binding protein were detected in 0.1 M CaCl2 extracts of Angelica dahurica L. suspension-cultured cells and carrot (Daucus carota L.) suspension-cultured cells, respectively, using a biotinylated cauliflower CaM gel-overlay technique in the presence of ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00262636
更新日期:1996-04-01 00:00:00
abstract::In yeast and mammals, ATP-dependent chromatin remodelling complexes of the SWI/SNF family play critical roles in the regulation of transcription, cell proliferation, differentiation and development. Homologues of conserved subunits of SWI/SNF-type complexes, including Snf2-type ATPases and SWI3-type proteins, particip...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-0915-5
更新日期:2009-05-01 00:00:00
abstract::The aim of this work was to examine the consequences of the heterologous expression of a spinach ( Spinacia oleracea L.) sucrose transporter ( SoSUT1) in potato ( Solanum tuberosum L.). Many studies have indicated that reduction of the expression of this class of sucrose transporter has deleterious effects on plant gr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-003-0975-x
更新日期:2003-05-01 00:00:00
abstract::Red light (R) and gibberellins (GA) each induce a water potential decrease in the axes of lettuce (Lactuca sativa L.) embryos resulting in germination of intact "seeds" (achenes) or an increase in growth of the axes of isolated embryos. The fruit coat and endosperm are a substantial barrier to the penetration of exoge...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391184
更新日期:1981-06-01 00:00:00
abstract::cis-Zeatin riboside was identified in transfer-RNA hydrolysates from both normal and crown-gall tissue of Vinca rosea L. The trans-isomer was associated exclusively with the crowngall transfer-RNA. The importance of these observations is discussed in relation to biosynthesis of free cytokinins. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392990
更新日期:1983-10-01 00:00:00
abstract::Membranes from the buds of Pisum sativum L. contain a protein kinase which is activated 5- to 15-fold by micromolar levels of calcium. Best calcium activations were found with light-membrane fractions, and on density gradients these band at a similar position to the plasma membrane. Other heavier membranes, however, a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394571
更新日期:1984-07-01 00:00:00
abstract::Assimilates entering the developing rice caryopsis traverse a short-distance pathway between the terminal sieve elements of the pericarp vascular bundle and the aleurone layer. The ultrastructure of this pathway has been studied. Sieve elements in the pericarp vascular bundle are smaller than their companion cells.The...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387436
更新日期:1981-05-01 00:00:00
abstract::Five major soluble nuclear proteins associated with cell proliferation were identified in Allium cepa L. root cells. One of them, of 64 kDa, was revealed by Western blotting with anti-mammalian nucleolin antibodies. A polyclonal antibody raised against this protein, which we have named NopA64, localised it in the nucl...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050093
更新日期:1997-01-01 00:00:00
abstract::In addition to membrane translocation, measured in the dark, it was found that pre-illumination of the chloroplasts resulted in an enhancement of sulfate uptake by 25% and of sulfite uptake by 55% as soon as the concentration of the ion in the incubation medium exceeded 2 mmol l(-1). This amount which is additionally ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388167
更新日期:1977-01-01 00:00:00
abstract:MAIN CONCLUSION:Although grass pea is an environmentally successful robust legume with major traits of interest for food and nutrition security, the genetic potential of this orphan crop has long been neglected. Grass pea (Lathyrus sativus L.) is a Neolithic plant that has survived millennia of cultivation and has spre...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-018-03084-0
更新日期:2019-09-01 00:00:00
abstract::We have investigated the levels of cell-specific expression of wheat-germ agglutinin (WGA) during the development of embryos and in 3-d-old seedlings. Southern blot analysis of genomic DNA derived from hexaploid and diploid wheat (Triticum) species indicates that the isolated cDNA (complementary DNA) clone is specific...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395422
更新日期:1988-12-01 00:00:00
abstract::Plant HXXXD acyltransferase-catalyzed malonylation is an important modification reaction in elaborating the structural diversity of flavonoids and anthocyanins, and a universal adaptive mechanism to detoxify xenobiotics. Nicotiana tabacum malonyltransferase 1 (NtMaT1) is a member of anthocyanin acyltransferase subfami...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1660-8
更新日期:2012-09-01 00:00:00
abstract::Lipid synthesis was studied in successive leaf sections from the base to the tip of developing barley (Hordeum vulgare L.), maize (Zea mays L.), rye grass (Lolium perenne L.) and wheat (Triticum aestivum L.) leaves. The endogenous levels of acyl lipids and their constituent fatty acids from the same leaf sections were...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388640
更新日期:1978-01-01 00:00:00
abstract:MAIN CONCLUSION:Infection by apple latent spherical virus (ALSV) vectors that promote the expression of Arabidopsis thaliana FLOWERING LOCUS T ( AtFT ) or Gentiana triflora GtFT s accelerates flowering in gentian and lisianthus plants. Apple latent spherical virus (ALSV) has isometric virus particles (25 nm in diameter...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2498-2
更新日期:2016-07-01 00:00:00
abstract::A bioassay for the inhibition of flowering involving the in vitro culture of excised, partially-induced, apices of Viscaria candida is described. This bioassay has been used to detect flowering inhibition in extracts from Kalanchoe blossfeldiana. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387363
更新日期:1972-06-01 00:00:00
abstract::The denaturation of Euglena gracilis in aggressive acids has been investigated by means of the chlorophyll oxidation. A comparative study in different halogen acetic acids revealed that the reaction kinetics are determined by a reaction between the acid anion and the outer membrane. The denaturation of cell membranes ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398261
更新日期:1976-01-01 00:00:00
abstract::Sieve-tube plastids of Aristolochia (5 species investigated) contain several starch grains and always one large crystalloid. In Asarum (3 species investigated) starch has not been found in the sieve-tubes. Their plastids contain several cuneate crystalloids that are sometimes arranged around an invisible centre. Asaru...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388406
更新日期:1971-03-01 00:00:00
abstract::Cells of Chlamydomonas reinhardtii Dang. wild type and the cell-wall mutants cw2 and cw15 were grown synchronously. The two mutants secreted copious amounts of cell-wall-like glycoproteins into the culture medium in contrast to the wild type which released only minor quantities. Both the secreted proteins as well as t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00194001
更新日期:1990-01-01 00:00:00
abstract::Glycoproteins extracted from microsomes of in-vitro-cultured cells of Daucus carota L. cv. US-Harumakigosun were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by peroxidase-conjugated concanavalin A. The appearance of a glycoprotein with Mr 31 000 (GP 31) was correlated with the ab...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00401030
更新日期:1988-03-01 00:00:00
abstract:MAIN CONCLUSION:Xyloglucan endo-transglycosylase/hydrolase ( Ph XET/H) regulates Podophyllum seed germination via GA mediated up-accumulation of Ph XET protein and subsequent endosperm weakening. Xyloglucan endo-transglycosylase/hydrolase (XET/H) belong to glycosyl hydrolase family 16, which play an important role in e...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2520-8
更新日期:2016-08-01 00:00:00
abstract::A cDNA clone was isolated from tree peony (Paeonia suffruticosa) subtractive cDNA library of burst buds and characterized with regard to its sequence, expression in response to chilling treatment during the release of bud dormancy, and its function in transgenic Arabidopsis thaliana. The clone, designated as PsMPT, co...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0757-6
更新日期:2008-09-01 00:00:00
abstract::Polysomes in sieve elements of rice (Oryza sativa L.) were studied with the electron microscope. The polysomes were found on the rough endoplasmic reticulum (ER) present in immature sieve elements and also on the cisternae of aggregated ER in the parietal layer of mature, enucleate sieve elements. In the immature siev...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390834
更新日期:1980-11-01 00:00:00
abstract::Benzoic acids are precursors of xanthone biosynthesis which has been studied in cell cultures of Hypericum androsaemum (Hypericaceae) and Centaurium erythraea (Gentianaceae). In both cell cultures, methyl jasmonate induces the intracellular accumulation of a new xanthone. Under these inductive conditions, feeding expe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000394
更新日期:2001-01-01 00:00:00