Isolation of functional extensor and flexor protoplasts fromPhaseolus coccineus L. pulvini: potassium induced swelling.

Abstract:

:Methods are described for the isolation of functional protoplasts from secondary pulvinus tissue (flexor and extensor) and from leaf mesophyll tissue of primary leaves ofPhaseolus coccineus L. Integrity of the protoplasts was shown by vital staining and their ability to evolve oxygen in the light. Extensor-cell protoplasts increased their volume for up to 60% upon addition of 10 mM KCl. This K(+)-induced swelling was accompanied by increased rates of proton extrusion.

journal_name

Planta

journal_title

Planta

authors

Erath F,Ruge WA,Mayer WE,Hampp R

doi

10.1007/BF00958956

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

447-52

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

173

pub_type

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