Abstract:
:Methods are described for the isolation of functional protoplasts from secondary pulvinus tissue (flexor and extensor) and from leaf mesophyll tissue of primary leaves ofPhaseolus coccineus L. Integrity of the protoplasts was shown by vital staining and their ability to evolve oxygen in the light. Extensor-cell protoplasts increased their volume for up to 60% upon addition of 10 mM KCl. This K(+)-induced swelling was accompanied by increased rates of proton extrusion.
journal_name
Plantajournal_title
Plantaauthors
Erath F,Ruge WA,Mayer WE,Hampp Rdoi
10.1007/BF00958956subject
Has Abstractpub_date
1988-12-01 00:00:00pages
447-52issue
4eissn
0032-0935issn
1432-2048journal_volume
173pub_type
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