Sex-specific binding and inactivation of agglutination factor in Chlamydomonas eugametos.

Abstract:

:Gametes of opposite mating type (mt (+) and mt (-)) of the green alga Chlamydomonas eugametos agglutinate via their flagella as a prelude to sexual fusion. To quantitate sexual agglutination, an in vitro assay has been developed using (35)S-labeled flagella and the isolated mt (-)agglutination factor. It is shown that not only isolated flagella, but also the mt (-)agglutination factor rapidly bind to the flagella of intact gametes of the opposite mating type. This confirms the role of the mt (-)agglutination factor in determining the sexual agglutinability of mt (-)gametes. As a function of binding, the agglutinative power of the flagella of both mating types is destroyed by a temperature-sensitive process. Likewise, the mt (-)agglutination factor can be completely inactivated.

journal_name

Planta

journal_title

Planta

authors

Fijst HL,Ossendorp FA,van Egmond P,Kamps AM,Musgrave A,van den Ende H

doi

10.1007/BF00411141

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

529-35

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

160

pub_type

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