Abstract:
:Gametes of opposite mating type (mt (+) and mt (-)) of the green alga Chlamydomonas eugametos agglutinate via their flagella as a prelude to sexual fusion. To quantitate sexual agglutination, an in vitro assay has been developed using (35)S-labeled flagella and the isolated mt (-)agglutination factor. It is shown that not only isolated flagella, but also the mt (-)agglutination factor rapidly bind to the flagella of intact gametes of the opposite mating type. This confirms the role of the mt (-)agglutination factor in determining the sexual agglutinability of mt (-)gametes. As a function of binding, the agglutinative power of the flagella of both mating types is destroyed by a temperature-sensitive process. Likewise, the mt (-)agglutination factor can be completely inactivated.
journal_name
Plantajournal_title
Plantaauthors
Fijst HL,Ossendorp FA,van Egmond P,Kamps AM,Musgrave A,van den Ende Hdoi
10.1007/BF00411141subject
Has Abstractpub_date
1984-05-01 00:00:00pages
529-35issue
6eissn
0032-0935issn
1432-2048journal_volume
160pub_type
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